Effects of seawater on growth and physiology of Betula microphylla var. paludosa cutting seedlings

doi:10.12302/j.issn.1000-2006.202004043

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (5): 102-108.doi: 10.12302/j.issn.1000-2006.202004043

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Effects of seawater on growth and physiology of Betula microphylla var. paludosa cutting seedlings

SHE Jianwei(), ZHANG Kang, ZHENG Xu, ZHAO Xiaojun, CHENG Fang, TANG Luozhong^*()

Co-Innovation Center for the Sustainable Forestry in Southern China,College of Forestry,Nanjing Forestry University,Nanjing 210037,China

Received:2020-04-21 Accepted:2020-10-12 Online:2021-09-30 Published:2021-09-30
Contact: TANG Luozhong E-mail:2389730488@qq.com;luozhongtang@njfu.edu.cn

Abstract

Abstract:

【Objective】 The aim of this research is to study the growth and physiological responses of Betula microphylla var. paludosa exposed to salt stress in seawater and provide the basis for the selection of seawater-tolerant tree species. 【Method】 Seawater diluted to different salinities (salt content) was used for root irrigation and leaf spray treatment of 1.5-year-old B. microphylla var. paludosa cutting seedlings. The root irrigating salinity was 0% (CK), 0.2%, 0.4% and 0.6% (mass fraction, same as below), respectively. The salinity of spray treatment on the leaves was 0 (CK), 3.07, 6.14, and 15.35 g/L, respectively. We observed morphological changes in the cutting seedlings and measured their growth and physiological indexes. 【Result】 When the salinity of the soil seawater treatment was below 0.6%, and the salinity of the leaf seawater treatment was below 6.14 g/L, the cutting seedlings suffered less salt damage and survived completely. Low salinity (0.2%) of seawater treatment on the soil could increase the content of chlorophyll and soluble protein in leaves to a certain extent and promote the growth of cutting seedlings. Both seawater treatments on the soil and the leaves could lead to an increase in the relative conductivity of leaves, but the increase in seawater treatment on the leaves had a more significant effect than that on the soil. The malondialdehyde (MDA) content of leaves under seawater treatment on the roots was lower than that of the control treatment (CK), and the MDA content of leaves under the seawater treatment on the leaves was higher than that of CK, but the difference between them was not significant. In addition to the high salinity (15.35 g/L) of seawater treatment on the leaves, the activity of superoxide dismutase in the leaves of other seawater treatments was lower than that of CK. After seawater treatment of the soil and leaves, the peroxidase activity of cutting seedling leaves was higher than that of CK in the early stage and lower than that of CK in the later stage. 【Conclusion】 B. microphylla var. paludosa could withstand soil seawater treatment with a salinity of 0.6% or leaf seawater treatment with a salinity of 6.14 g/L, showing a strong tolerance to seawater, and had substantial application potential in saline land greening.

Key words: Betula microphylla var. paludosa, salt stress, seawater treatment, seedling growth, physiology

CLC Number:

S718

SHE Jianwei, ZHANG Kang, ZHENG Xu, ZHAO Xiaojun, CHENG Fang, TANG Luozhong. Effects of seawater on growth and physiology of Betula microphylla var. paludosa cutting seedlings[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(5): 102-108.

Figures/Tables 4

Table 1

Table 2

Fig.1

Table 3

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处理时间/d treatment time	土壤盐度/% soil salinity				叶片盐度/(g·L^-1) leaf salinity
处理时间/d treatment time	CK	0.20	0.40	0.60	3.07	6.14	15.35
2	正常	正常	正常	正常	正常	正常	正常
6	正常	正常	正常	正常	正常	约1/4的叶片叶缘枯萎	约3/4的叶片叶缘枯萎
14	正常	正常	正常	正常	正常	约1/4的叶片叶缘枯萎,轻微落叶	约4/5的叶片枯萎,严重落叶, 植株下部萌发出较小的新叶
30	正常	正常	下部有少量叶片枯萎脱落	下部有较多叶片枯萎脱落	正常	下部部分老叶脱落, 萌发少量新叶	留有少量老叶,新叶较多但比较小,部分苗木顶梢枯萎

海水处理 treatment	盐度 salinity	存活率/ % survival rate	苗高增量/cm height increment	地径增量/mm ground diameter increment	单株生物量/g biomass
土壤浇灌 soil irrigating	CK	100.0	7.10±0.90 a	0.76±0.14 a	8.62±1.85 a
	0.2%	100.0	6.00±0.82 a	0.80±0.10 a	9.67±1.72 a
	0.4%	100.0	3.50±0.41 b	0.67±0.05 a	7.63±1.45 a
	0.6%	100.0	3.33±0.82 b	0.42±0.04 b	7.04±1.08 a
叶片喷雾 leaf spary	CK	100.0	7.10±0.90 a	0.76±0.14 a	8.62±1.85 a
	3.07 g/L	100.0	6.20±0.86 ab	0.64±0.13 ab	7.08±1.43 a
	6.14 g/L	100.0	6.04±1.87 ab	0.50±0.14 ab	7.52±1.26 a
	15.35 g/L	87.5	4.90±0.73 b	0.45±0.10 b	6.77±0.07 a

指标 index	叶绿素含量 chlorophyll content	可溶性蛋白含量 soluble protein content	相对电导率 relative conductivity	MDA含量 MDA content	SOD活性 SOD activity	POD活性 POD activity
叶绿素含量chlorophyll content	1.000
可溶性蛋白含量soluble protein content	0.265	1.000
相对电导率relative conductivity	0.019	0.092	1.000
MDA含量MDA content	-0.478^**	-0.392^*	0.064	1.000
SOD活性SOD activity	0.059	-0.188	0.479^**	0.430^*	1.000
POD活性POD activity	0.280	-0.055	0.204	-0.209	0.055	1.000

Effects of seawater on growth and physiology of Betula microphylla var. paludosa cutting seedlings

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