JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (3): 87-92.doi: 10.12302/j.issn.1000-2006.202005006
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CHEN Xingbin1(), XU Haining1, XIAO Fuming1,*(
), SUN Shiwu2, LOU Yongfeng1, ZOU Yuanxi3, XU Xiaoqiang1
Received:
2020-05-06
Revised:
2020-09-22
Online:
2021-05-30
Published:
2021-05-31
Contact:
XIAO Fuming
E-mail:xingbinsd@163.com;jxxiaofuming@163.com
CLC Number:
CHEN Xingbin, XU Haining, XIAO Fuming, SUN Shiwu, LOU Yongfeng, ZOU Yuanxi, XU Xiaoqiang. Genetic diversity and paternity analyses in a 1.5th generation seed orchard of Chenshan red-heart Chinese fir[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(3): 87-92.
Table 1
Genetic diversity estimates for the parent population and the progeny population"
位点 locus | Na | Ne | I | Ho | He |
---|---|---|---|---|---|
H7 | 3(5) | 1.814 (1.863) | 0.768 (0.779) | 0.405 (0.422) | 0.455 (0.464) |
H8 | 3(4) | 2.128 (2.150) | 0.825 (0.847) | 0.526 (0.534) | 0.537 (0.535) |
H16 | 4(5) | 1.958 (1.882) | 0.915 (0.947) | 0.447 (0.500) | 0.496 (0.469) |
H33 | 3(5) | 1.833 (1.681) | 0.723 (0.707) | 0.378 (0.385) | 0.461 (0.405) |
H76 | 6(11) | 2.488 (2.420) | 1.121 (1.101) | 0.833 (0.599) | 0.606 (0.587) |
H97 | 6(8) | 3.389 (3.411) | 1.419 (1.434) | 0.730 (0.628) | 0.715 (0.708) |
H193 | 4(8) | 2.180 (1.953) | 0.939 (0.864) | 0.552 (0.492) | 0.548 (0.489) |
H201 | 5(5) | 2.039 (1.982) | 0.943 (0.946) | 0.649 (0.482) | 0.517 (0.496) |
H286 | 5(8) | 2.009 (2.256) | 0.943 (1.067) | 0.556 (0.563) | 0.509 (0.558) |
LX-13 | 3(5) | 1.866 (1.680) | 0.734 (0.706) | 0.405 (0.390) | 0.471 (0.405) |
LX-18 | 7(9) | 3.457 (3.916) | 1.405 (1.504) | 0.784 (0.734) | 0.721 (0.745) |
LX-55 | 4(5) | 2.796 (2.48) | 1.173 (1.151) | 0.583 (0.567) | 0.651 (0.597) |
平均mean | 4.41 (6.50) | 2.330 (2.306) | 0.992 (1.004) | 0.571 (0.525) | 0.557 (0.538) |
Table 2
Outcrossing rate of 14 families"
家系号 family No. | 分析 子代数 offspring amount analyzed | tm | ts | tm-ts |
---|---|---|---|---|
5 | 31 | 1.200(0.002) | 1.200(0.002) | 0.000(0.000) |
7 | 33 | 1.200(0.002) | 1.163(0.000) | 0.037(0.000) |
8 | 33 | 1.002(0.001) | 1.115(0.000) | -0.113(0.000) |
10 | 34 | 1.200(0.002) | 1.056(0.000) | 0.144(0.000) |
12 | 32 | 1.200(0.002) | 1.050(0.000) | 0.150(0.000) |
14 | 35 | 1.114(0.025) | 1.105(0.045) | 0.009(0.055) |
16 | 33 | 1.200(0.002) | 1.077(0.001) | 0.123(0.000) |
19 | 30 | 1.200(0.002) | 1.056(0.002) | 0.144(0.000) |
27 | 30 | 1.200(0.002) | 1.200(0.002) | 0.000(0.000) |
29 | 34 | 1.200(0.002) | 1.200(0.002) | 0.000(0.000) |
41 | 33 | 1.092(0.000) | 1.087(0.000) | 0.005(0.000) |
45 | 35 | 1.200(0.002) | 1.057(0.001) | 0.143(0.000) |
48 | 33 | 1.200(0.002) | 1.075(0.001) | 0.125(0.000) |
51 | 33 | 0.938(0.000) | 1.065(0.000) | -0.127(0.000) |
Table 3
The analysis of the paternal origin of 14 families"
家系号 family No. | 分析子 代数 offspring amount analyzed | 确定父本 的子代数 offspring number determined male parent | 确定父本 的子代数 比率/% rate of offspring determined male parent | 父本个数 number of male parent | 异交率/% outcrossing rate |
---|---|---|---|---|---|
5 | 31 | 19 | 61.3 | 9 | 100 |
7 | 33 | 27 | 81.8 | 11 | 100 |
8 | 33 | 30 | 90.9 | 7 | 100 |
10 | 34 | 30 | 88.2 | 8 | 100 |
12 | 32 | 26 | 81.3 | 11 | 100 |
14 | 35 | 19 | 54.3 | 9 | 100 |
16 | 33 | 18 | 54.5 | 13 | 100 |
19 | 30 | 21 | 70.0 | 8 | 100 |
27 | 30 | 17 | 56.7 | 7 | 100 |
29 | 34 | 24 | 70.6 | 11 | 100 |
41 | 33 | 31 | 93.9 | 11 | 100 |
45 | 35 | 19 | 54.3 | 10 | 100 |
48 | 33 | 20 | 60.6 | 13 | 100 |
51 | 33 | 24 | 72.7 | 13 | 100 |
[1] | GONZAGA J, MANOEL R, SOUSA A, et al. Pollen contamination and nonrandom mating in a Eucalyptus camaldulensis Dehnh seedling seed orchard[J]. Silvae Genet, 2016,65:1-11. DOI: org/10.1515/sg-2016-0001. |
[2] |
HANSEN O K, KJAER E D. Paternity analysis with microsatellites in a Danish Abies nordmanniana clonal seed orchard reveals dysfunctions[J]. Can J For Res, 2006,36(4):1054-1058.DOI: 10.1139/x05-299.
doi: 10.1139/x05-299 |
[3] |
FERNANDES L, ROCHETA M, CORDEIRO J, et al. Genetic variation,mating patterns and gene flow in a Pinus pinaster Aiton clonal seed orchard[J]. Ann For Sci, 2008,65(7):706.DOI: 10.1051/forest:2008049.
doi: 10.1051/forest:2008049 |
[4] |
PLOMION C, LEPROVOST G, POT D, et al. Pollen contamination in a maritime pine polycross seed orchard and certification of improved seeds using chloroplast microsatellites[J]. Can J For Res, 2001,31(10):1816-1825.DOI: 10.1139/x01-115.
doi: 10.1139/x01-115 |
[5] |
DUREL C E, BERTIN P, KREMER A. Relationship between inbreeding depression and inbreeding coefficient in maritime pine (Pinus pinaster)[J]. Theor Appl Genet, 1996,92(3):347-356.DOI: 10.1007/bf00223678.
doi: 10.1007/BF00223678 |
[6] |
DERING M, MISIORNY A, CHALUPKA W. Inter-year variation in selfing,background pollination,and paternal contribution in a Norway spruce clonal seed orchard[J]. Can J For Res, 2014,44(7):760-767.DOI: 10.1139/cjfr-2014-0061.
doi: 10.1139/cjfr-2014-0061 |
[7] | 曾志光, 杨先锋, 肖复明, 等. 陈山红心杉材性变异及其基因资源利用的研究[J]. 江西林业科技, 2001,29(3):1-6,39. |
ZENG Z G, YANG X F, XIAO F M, et al. Study on variability of timber characters and gene resources utilization for Chenshan red-heart Chinese fir[J]. Jiangxi For Sci Technol, 2001,29(3):1-6,39.DOI: 10.16259/j.cnki.36-1342/s.2001.03.001. | |
[8] | 葛艺早, 刘文飞, 吴建平, 等. 不同施肥处理对杉木种子园种子品质的影响[J]. 森林与环境学报, 2016,36(4):442-448. |
GE Y Z, LIU W F, WU J P, et al. Effects of different fertilization treatments on quality of the seeds in a third generation seed orchard of Cunninghamia lanceolata[J]. J For Environ, 2016,36(4):442-448.DOI: 10.13324/j.cnki.jfcf.2016.04.010. | |
[9] | 郑仁华. 杉木种子园自由授粉子代遗传变异及优良遗传型选择[J]. 南京林业大学学报(自然科学版), 2006,30(1):8-12. |
ZHENG R H. Genetic variations of seed orchard open-pollinated progenies and selection of superior genotypes of Chinese fir[J]. J Nanjing For Univ (Nat Sci Ed), 2006,30(1):8-12.DOI: 10.3969/j.issn.1000-2006.2006.01.002. | |
[10] | 陈兴彬, 何龙燕, 肖复明, 等. 基于转录组测序的陈山红心杉EST-SSR开发及应用[J]. 中南林业科技大学学报, 2020,40(8):120-127. |
CHEN X B, HE L Y, XIAO F M, et al. Development and application of EST-SSR markers in Chenshan red-heart Chinese fir based on transcriptome sequencing[J]. J Central South Univ For Technol, 2020,40(8):120-127.DOI: 10.14067/j.cnki.1673-923x.2020.08.015. | |
[11] |
RITLAND K. Extensions of models for the estimation of mating systems using n independent loci[J]. Heredity, 2002,88(4):221-228.DOI: 10.1038/sj.hdy.6800029.
doi: 10.1038/sj.hdy.6800029 |
[12] |
SUN M, RITLAND K. Mating system of yellow starthistle (Centaurea solstitialis),a successful colonizer in north America[J]. Heredity, 1998,80(2):225-232.DOI: 10.1046/j.1365-2540.1998.00290.x.
doi: 10.1046/j.1365-2540.1998.00290.x |
[13] |
KALINOWSKI S T, TAPER M L, MARSHALL T C. Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment[J]. Mol Ecol, 2007,16(5):1099-1106.DOI: 10.1111/j.1365-294X.2007.03089.x.
doi: 10.1111/j.1365-294X.2007.03089.x |
[14] | 乔东亚, 王鹏, 王淑安, 等. 基于SNP标记的紫薇遗传多样性分析[J]. 南京林业大学学报(自然科学版), 2020,44(4):21-28. |
QIAO D Y, WANG P, WANG S A, et al. Genetic diversity analysis of Lagerstroemia germplasm resources based on SNP markers[J]. J Nanjing For Univ (Nat Sci Ed), 2020,44(4):21-28. DOI: 10.3969/j.issn.1000-2006.202003075. | |
[15] | 艾畅, 徐立安, 赖焕林, 等. 马尾松种子园的遗传多样性与父本分析[J]. 林业科学, 2006,42(11):146-150. |
AI C, XU L A, LAI H L, et al. Genetic diversity and paternity analysis of a seed orchard in Pinus massoniana[J]. Sci Silvae Sin, 2006,42(11):146-150.DOI: 10.3321/j.issn:1001-7488.2006.11.026. | |
[16] | 于大德, 袁定昌, 张登荣, 等. 华北落叶松种子园不同世代间遗传多样性变化[J]. 植物遗传资源学报, 2014,15(5):940-947. |
YU D D, YUAN D C, ZHANG D R, et al. Genetic diversity of Larix principis-rupprechtii Mayr.seed orchard among generations[J]. J Plant Genet Resour, 2014,15(5):940-947.DOI: 10.13430/j.cnki.jpgr.2014.05.004. | |
[17] | 赵奋成, 郭文冰, 林昌明, 等. 不同近交程度对湿地松结实与生长的影响[J]. 南京林业大学学报(自然科学版), 2019,43(1):9-17. |
ZHAO F C, GUO W B, LIN C M, et al. Effects of different inbreeding levels on seed characteristics and growth of slash pine[J]. J Nanjing For Univ (Nat Sci Ed), 2019,43(1):9-17.DOI: 10.3969/j.issn.1000-2006.201712043. | |
[18] | 谭小梅, 周志春, 金国庆, 等. 马尾松二代无性系种子园遗传多样性和交配系统分析[J]. 林业科学, 2012,48(2):69-74. |
TAN X M, ZHOU Z C, JIN G Q, et al. Genetic diversity and mating system analysis of Pinus massoniana in a second-generation clonal seed orchard[J]. Sci Silvae Sin, 2012,48(2):69-74. | |
[19] |
MORIGUCHI Y, YAMAZAKI Y, TAIRA H, et al. Mating patterns in an indoor miniature Cryptomeria japonica seed orchard as revealed by microsatellite markers[J]. New For, 2010,39(3):261-273.DOI: 10.1007/s11056-009-9169-0.
doi: 10.1007/s11056-009-9169-0 |
[20] | 叶培忠, 陈岳武, 蒋恕, 等. 杉木种子生活力变异的研究[J]. 南京林业大学学报, 1981,5(3):22-32. |
YE P Z, CHEN Y W, JIANG S, et al. A preliminary study on the variation of seed vigor of Chinese fir[J]. J Nanjing For Univ, 1981,5(3):22-32. | |
[21] |
GONZÁLEZ-MARTÍNEZ S C, GERBER S, CERVERA M T, et al. Selfing and sibship structure in a two-cohort stand of maritime pine (Pinus pinaster Ait.) using nuclear SSR markers[J]. Ann For Sci, 2003,60(2):115-121.DOI: 10.1051/forest:2003003.
doi: 10.1051/forest:2003003 |
[22] |
DE-LUCAS A I, ROBLEDO-ARNUNCIO J J, HIDALGO E, et al. Mating system and pollen gene flow in Mediterranean maritime pine[J]. Heredity (Edinb), 2008,100(4):390-399.DOI: 10.1038/sj.hdy.6801090.
doi: 10.1038/sj.hdy.6801090 |
[23] |
GRATTAPAGLIA D, AMARAL DIENER P S, SANTOS G A. Performance of microsatellites for parentage assignment following mass controlled pollination in a clonal seed orchard of loblolly pine (Pinus taeda L.)[J]. Tree Genet Genomes, 2014,10(6):1631-1643.DOI: 10.1007/s11295-014-0784-3.
doi: 10.1007/s11295-014-0784-3 |
[24] |
DERING M, MISIORNY A, CHALUPKA W. Inter-year variation in selfing,background pollination,and paternal contribution in a Norway spruce clonal seed orchard[J]. Can J For Res, 2014,44(7):760-767.DOI: 10.1139/cjfr-2014-0061.
doi: 10.1139/cjfr-2014-0061 |
[25] |
CHEN X B, SUN X M, DONG L M, et al. Mating patterns and pollen dispersal in a Japanese larch (Larix kaempferi) clonal seed orchard:a case study[J]. Sci China Life Sci, 2018,61(9):1011-1023.DOI: 10.1007/s11427-018-9305-7.
doi: 10.1007/s11427-018-9305-7 |
[26] |
SHIMONO A, WANG X R, TORIMARU T, et al. Spatial variation in local pollen flow and mating success in a Picea abies clone archive and their implications for a novel “breeding without breeding” strategy[J]. Tree Genet Genomes, 2011,7(3):499-509.DOI: 10.1007/s11295-010-0351-5.
doi: 10.1007/s11295-010-0351-5 |
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