Contents of the total saponins and total flavonoids in different organs of Sapindus mukorossi

doi:10.12302/j.issn.1000-2006.202006060

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (4): 83-89.doi: 10.12302/j.issn.1000-2006.202006060

Contents of the total saponins and total flavonoids in different organs of Sapindus mukorossi

XU Yuanyuan¹(), ZHOU Siwei¹, CHEN Zhong¹, ZHAO Guochun¹, LIU Jiming¹, WANG Lixian¹, WANG Xi¹, JIA Liming¹^,^*(), ZHANG Duanguang²

1. Key Laboratory of Silviculture and Conservation of the Ministry of Education, National Energy R&D Center for Non-food Biomass, College of forestry, Beijing Forestry University, Beijing 100083, China
2. Yuanhua Forestry Biotechnology Co., Ltd., Jianning 354500, China

Received:2020-06-30 Accepted:2020-08-08 Online:2021-07-30 Published:2021-07-30
Contact: JIA Liming E-mail:yuanyuanxu_2016@163.com;jlm@bjfu.edu.cn

Abstract

Abstract:

【Objective】Our aim was to study the contents of total saponins and total flavonoids in different organs, and their accumulation characteristics, in the leaves of soapberry (Sapindus mukorossi). We also aimed to analyze the correlations between them. This was done to provide a reference for the comprehensive and efficient utilization of S. mukorossi.【Method】The contents of total saponins and flavonoids in the flowers, fruits, branches, roots and leaves at eight growth stages of S. mukorossi were determined by spectrophotometry. 【Result】The total saponin and total flavonoid contents varied among the different organs. The levels of the total saponins in different organs (0.98%-13.26%) were significantly higher than those of the total flavonoids (0.31%-1.74%). The total saponin content in the pericarp was the highest (13.26%), followed by that in the flowers (5.68%-6.21%) and leaves (4.11%, the average of eight growth stages). The order of total flavonoid content in each organ was as follows: leaf (1.74%, the average of eight growth stages), flower bud (0.88%), flower (0.74%), root (0.53%), branch (0.44%) and pericarp (0.31%). The accumulation rules of the total saponins and total flavonoids in the leaves were different at different stages of the annual growth cycle. Overall, the total saponin content increased gradually and the total flavonoid content fluctuated. Furthermore, there was a negative correlation between the total saponin and total flavonoid content. 【Conclusion】The distribution of the total saponins and total flavonoids in different organs, and their accumulation characteristics in leaves, are different. Moreover, here may be an indirect competitive relationship between the accumulation of saponins and flavonoids. In addition to the pericarp, flowers and leaves are the main organs for the accumulation of saponins and flavonoids, which have a certain application value that can be reasonably collected and utilized.

Key words: soapberry (Sapindus mukorossi), organ, saponins, flavonoids

CLC Number:

S567

XU Yuanyuan, ZHOU Siwei, CHEN Zhong, ZHAO Guochun, LIU Jiming, WANG Lixian, WANG Xi, JIA Liming, ZHANG Duanguang. Contents of the total saponins and total flavonoids in different organs of Sapindus mukorossi[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(4): 83-89.

Figures/Tables 4

References 42

[1]	黎阳, 张铁军, 刘素香, 等. 人参化学成分和药理研究进展[J]. 中草药, 2009, 40(1):164-166.
	LI Y, ZHANG T J, LIU S X, et al. Advances in chemical constituents and pharmacology of Ginseng[J]. Chin Tradit Herb Drugs, 2009, 40(1):164-166. DOI: 10.3321/j.issn:0253-2670.2009.01.049. doi: 10.3321/j.issn:0253-2670.2009.01.049
[2]	李行诺, 翟文丰, 周孟宇, 等. 黄柏化学成分研究[J]. 浙江工业大学学报, 2012, 40(3):244-246.
	LI X N, ZHAI W F, ZHOU M Y, et al. Chemical constituents from Phellodendron chinense[J]. Zhejiang Univ Technol, 2012, 40(3):244-246. DOI: 10.3969/j.issn.1006-4303.2012.03.002. doi: 10.3969/j.issn.1006-4303.2012.03.002
[3]	PAN X, DU L, TAO J, et al. Dynamic changes of flavonoids in Abelmoschus manihot different organs at different growth periods by UPLC-MS/MS[J]. J Chromatogr B Analyt Technol Biomed Life Sci, 2017, 1059:21-26. DOI: 10.1016/j.jchromb.2017.05.020. doi: 10.1016/j.jchromb.2017.05.020
[4]	米丽雪, 上官新晨, 施利仙, 等. 青钱柳营养器官总黄酮含量测定及分布规律研究[J]. 江西农业大学学报(自然科学版), 2009, 31(5):896-900.
	MI L X, SHANGGUAN X C, SHI L X, et al. Studies on the determination and distributon of total flavono ids of Cyclocarya paliurus vegetative organs[J]. Acta Agric Univ Jiangxiensis (Nat Sci Ed), 2009, 31(5):896-900. DOI: 10.3969/j.issn.1000-2286.2009.05.024. doi: 10.3969/j.issn.1000-2286.2009.05.024
[5]	LI J, HU Z. Accumulation and dynamic trends of triterpenoid saponin in vegetative organs of Achyranthus bidentata[J]. J Integr Plant Biol, 2009, 51(2):122-129. DOI: 10.1111/j.1744-7909.2008.00764.x. doi: 10.1111/j.1744-7909.2008.00764.x
[6]	赵昶灵, 陈中坚, 余育启, 等. 三七紫、绿茎植株营养器官在不同生长阶段的花色苷和皂苷含量及其相关性[J]. 中药材, 2016, 39(9):1923-1929.
	ZHAO C L, CHEN Z J, YU Y Q, et al. Contents and the correlations of the total anthocyanins and sa-ponins of the vegetative organs of Panax notoginseng with purple and green aerial stems at different growth stages[J]. J Chin Med Mater, 2016, 39(9):1923-1929. DOI: 10.13863/j.issn1001-4454.2016.09.001. doi: 10.13863/j.issn1001-4454.2016.09.001
[7]	高玲玲, 刘文哲, 张兴旺. 皂苷元在远志不同部位的含量分布及其动态变化研究[J]. 分子植物育种, 2019, 17(15):5131-5135.
	GAO L L, LIU W Z, ZHANG X W. Study on the content distribution and dynamic changes of senegenin in different parts of Polygala tenuifolia[J]. Molecular Plant Breeding, 2019, 17(15):5131-5135.
[8]	谭玲玲, 蔡霞, 胡正海. 北柴胡各器官中总黄酮动态变化研究[J]. 中草药, 2008(2):286-287.
	TAN L L, CAI X, HU Z H, et al. Dynamic changes of total flavonoids in organs of Bupleurum chinense[J]. Chin Tradit Herb Drugs, 2008(2):286-287.
[9]	李娜, 陆嘉惠, 秦忠立, 等. 光果甘草营养器官不同季节总黄酮消长规律的研究[J]. 西北植物学报, 2012, 32(1):162-165.
	LI N, LU J H, QIN Z L, et al. Changes of total flavonoids content in vegetative organs of Glycyrrhiza glabra L.in different seasons[J]. Acta Bot Boreali-Occidentalia Sin, 2012, 32(1):162-165. DOI: 10.3969/j.issn.1000-4025.2012.01.026. doi: 10.3969/j.issn.1000-4025.2012.01.026
[10]	蔡爽, 阮成江, 杜维, 等. 沙棘叶片、果肉和种子中黄酮类成分的差异[J]. 植物资源与环境学报, 2019, 28(4):58-67.
	CAI S, RUAN C J, DU W, et al. Difference in flavonoid constituents in leaf,pulp,and seed of Hippophae rhamnoides[J]. J Plant Resour Environ, 2019, 28(4):58-67.DOI: 10.3969/j.issn.1674-7895.2019.04.07. doi: 10.3969/j.issn.1674-7895.2019.04.07
[11]	张兰兰, 张欣欣, 孙阎. 三花龙胆不同器官可溶性糖和龙胆苦苷含量的动态研究[J]. 中国农学通报, 2017, 33(9):78-83.
	ZHANG L L, ZHANG X X, SUN Y. Dynamic study on soluble sugar and gentiopicroside content in different organs of Gentiana triflora Pall.[J]. Chin Agric Sci Bull, 2017, 33(9):78-83.
[12]	练冬梅, 姚运法, 赖正锋, 等. 不同黄秋葵花的花青素和总黄酮含量分析[J]. 热带农业科学, 2018, 38(9):73-75.
	LIAN D M, YAO Y F, LAI Z F, et al. Determination of the contents of anthocyanin and total flavonoids in different okra flowers[J]. Chin J Trop Agric, 2018, 38(9):73-75. DOI: 10.12008/j.issn.1009-2196.2018.09.015. doi: 10.12008/j.issn.1009-2196.2018.09.015
[13]	中国植物志编辑委员会. 中国植物志: 第47卷第1分册[M]. 北京: 科学出版社, 1985.
[14]	刘济铭, 孙操稳, 何秋阳, 等. 国内外无患子属种质资源研究进展[J]. 世界林业研究, 2017, 30(6):12-18.
	LIU J M, SUN C W, HE Q Y, et al. Research progress in Sapindus L. germplasm resources[J]. World For Res, 2017, 30(6):12-18. DOI: 10.13348/j.cnki.sjlyyj.2017.0071.y. doi: 10.13348/j.cnki.sjlyyj.2017.0071.y
[15]	李时珍. 本草纲目[M]. 北京: 人民卫生出版社, 1975.
[16]	谢媛媛. 毛瓣无患子果皮、怀菊和石榴花生物活性成分的研究[D]. 沈阳: 沈阳药科大学, 2009.
	XIE Y Y. Studies on bio-active constituents of three natural medicines-pericarps of Sapindus rarak,flowers of Chrysanthemum morifolium Ramat.‘Huaiju’ cv Nov and Punica granatum[D]. Shenyang:Shenyang Pharmaceutical University, 2009.
[17]	刘济铭, 陈仲, 孙操稳, 等. 无患子属种质资源种实性状变异及综合评价[J]. 林业科学, 2019, 55(6):44-54.
	LIU J M, CHEN Z, SUN C W, et al. Variation in fruit and seed properties and comprehensive assessment of germplasm resources of the genus Sapindus[J]. Sci Silvae Sin, 2019, 55(6):44-54. DOI: 10.11707/j.1001-7488.20190606. doi: 10.11707/j.1001-7488.20190606
[18]	郑玉琳, 刘济铭, 史双龙, 等. 无患子果实成熟过程及其油脂、皂苷动态变化[J]. 南京林业大学学报(自然科学版), 2021, 45(4):76-82.
	ZHENG Y L, LIU J M, SHI S L, et al. Maturation process and the dynamics of oil and saponin in Sapindus mukorossi[J]. J Nanjing For Univ( Nat Sci Ed), 2021, 45(4) : 76-82.DOI: 10.12302/ j.issn.1000-2006.202103047. doi: 10.12302/ j.issn.1000-2006.202103047
[19]	UPADHYAY A, SINGH D K. Pharmacological effects of Sapindus mukorossi[J]. Rev Inst Med Trop Sao Paulo, 2012, 54(5):273-280. DOI: 10.1590/s0036-46652012000500007. doi: 10.1590/s0036-46652012000500007
[20]	NI W, HUA Y, LIU H Y, et al. Tirucallane-type triterpenoid sapo-nins from the roots of Sapindus mukorossi[J]. Chem Pharm Bull, 2006, 54(10):1443-1446. DOI: 10.1248/cpb.54.1443. doi: 10.1248/cpb.54.1443
[21]	WANG Y H, CAO X L, QIN B Y, et al. Four new tirucallane-type triterpenoids from Sapindus mukorossi Gaertn. flowers induced neurite outgrowth in PC12 cells related to insulin-like growth factor 1 receptor/phosphoinositide 3-kinase/extracellular regulated protein kinase signaling pathway[J]. Phytochem Lett, 2019, 34:5-12. DOI: 10.1016/j.phytol.2019.08.011. doi: 10.1016/j.phytol.2019.08.011
[22]	ZIKOVA N I, KRIVENCHUK P E. Chemical study of flavonoids from the leaves of Sapindus mukorossi Gaerth[J]. FarmatsevtychnyǏ Zhurnal, 1970, 25(5):43-45.
[23]	SHAH M, PARVEEN Z, KHAN M R. Evaluation of antioxidant, anti-inflammatory, analgesic and antipyretic activities of the stem bark of Sapindus mukorossi[J]. BMC Complement Altern Med, 2017, 17(1):526. DOI: 10.1186/s12906-017-2042-3. doi: 10.1186/s12906-017-2042-3
[24]	SUN C W, WANG J W, DUAN J, et al. Association of fruit and seed traits of Sapindus mukorossi germplasm with environmental factors in Southern China[J]. Forests, 2017, 8(12):491. DOI: 10.3390/f8120491. doi: 10.3390/f8120491
[25]	魏伯平, 张咏梅, 曹致中. 9种豆科牧草总黄酮和总皂苷积累规律的研究[J]. 草地学报, 2012, 20(1):88-95.
	WEI B P, ZHANG Y M, CAO Z Z. Accumulation patterns of total flavonoids and saponins in nine legume species[J]. Acta Agrectir Sin, 2012, 20(1):88-95. DOI: 10.11733/j.issn.1007-0435.2012.01.015. doi: 10.11733/j.issn.1007-0435.2012.01.015
[26]	CHUNGI M, MILLER D A. Natural herbicide potential of alfalfa residue on selected weed species[J]. Agron J, 1995, 87(5):920-925. DOI: 10.2134/agronj1995.00021962008700050024x. doi: 10.2134/agronj1995.00021962008700050024x
[27]	SAHA S, WALIA S, KUMAR J, et al. Screening for feeding deterrent and insect growth regulatory activity of triterpenic saponins from Diploknema butyracea and Sapindus mukorossi[J]. J Agric Food Chem, 2010, 58(1):434-440. DOI: 10.1021/jf902439m. doi: 10.1021/jf902439m
[28]	EDDAYA T, BOUGHDAD A, SIBILLE E, et al. Biological activity of Sapindus mukorossi Gaerten (Sapindaceae) aqueous extract against Thysanoplusia orichalcea (Lepidoptera: Noctuidae)[J]. Ind Crop Prod, 2013, 50:325-332. DOI: 10.1016/j.indcrop.2013.07.045. doi: 10.1016/j.indcrop.2013.07.045
[29]	PILBEAM D J. The flavonoids: advances in research[J]. FEBS Lett, 1983, 158(1):192.DOI: 10.1016/0014-5793(83)80717-0. doi: 10.1016/0014-5793(83)80717-0
[30]	AMBASHT N K, AGRAWAL M. Physiological responses of field growth Zea may L. plants to enhanced UV-B radiation[J]. Biotronics, 1995, 24(2):15-23.
[31]	MCCLURE J W. Physiology and functions of flavonoids[M]. Boston MA:Springer US, 1975:970-1055.DOI: 10.1007/978-1-4899-2909-9_18. doi: 10.1007/978-1-4899-2909-9_18
[32]	WANG W J, LI X Y, ZU Y G. Dynamic feature of flavonoids content in different organs of larch (Larix gmelinii)[J]. J For Res, 2005, 16(2):89-92. DOI: 10.1007/BF02857896. doi: 10.1007/BF02857896
[33]	TOH D F, NEW L S, KOH H L, et al. Ultra-high performance liquid chromatography/time-of-flight mass spectrometry (UHPLC/TOFMS) for time-dependent profiling of raw and steamed Panax notoginseng[J]. J Pharm Biomed Anal, 2010, 52(1):43-50. DOI: 10.1016/j.jpba.2009.12.005. doi: 10.1016/j.jpba.2009.12.005
[34]	XIA P G, GUO H B, RU M, et al. Accumulation of saponins in Panax notoginseng during its growing seasons[J]. Ind Crop Prod, 2017, 104:287-292. DOI: 10.1016/j.indcrop.2017.04.045. doi: 10.1016/j.indcrop.2017.04.045
[35]	庄向平, 虞杏英, 杨更生, 等. 银杏叶中黄酮含量的测定和提取方法[J]. 中草药, 1992, 23(3):122-124, 168.
	ZHUANG X P, YU X Y, YANG G S, et al. Determination of total flavonoids in the leaves of Ginkgo (Ginkgo bilcba) and studies on its extraction process[J]. Chin Tradit Herb Drugs, 1992, 23(3):122-124, 168.
[36]	朱冬雪, 顾采琴, 周伯春. 喜树不同生长发育期及不同器官中黄酮含量的变化[J]. 广东林业科技, 2012, 28(1):61-64.
	ZHU D X, GU C Q, ZHOU B C. Changes of flavonoid content in different organs of Camptotheca acuminata at different growth stages[J]. Guangdong For Sci Technol, 2012, 28(1):61-64.
[37]	ZHAO Y J, LI C. Biosynjournal of plant triterpenoid saponins in microbial cell factories[J]. J Agric Food Chem, 2018, 66(46):12155-12165. DOI: 10.1021/acs.jafc.8b04657. doi: 10.1021/acs.jafc.8b04657
[38]	FALCONE F M L, RIUS S P, CASATI P. Flavonoids: biosynjournal, biological functions, and biotechnological applications[J]. Front Plant Sci, 2012, 3:222-222. DOI: 10.3389/fpls.2012.00222. doi: 10.3389/fpls.2012.00222
[39]	韩建萍, 梁宗锁, 王敬民. 矿质元素与根类中草药根系生长发育及有效成分累积的关系[J]. 植物生理学通讯, 2003, 39(1):78-82.
	HAN J P, LIANG Z S, WANG J M. The relationship between mineral element and the root growth and accumulation of effective ingredient in root of traditional herbs[J]. Plant Physiol Commun, 2003, 39(1):78-82. DOI: 10.13592/j.cnki.ppj.2003.01.026. doi: 10.13592/j.cnki.ppj.2003.01.026
[40]	ZHAO G C, GAO Y H, GAO S L, et al. The phenological growth stages of Sapindus mukorossi according to BBCH scale[J]. Forests, 2019, 10(6):462. DOI: 10.3390/f10060462. doi: 10.3390/f10060462
[41]	蔡佳杰, 曹泳, 蔡邦红. 一种无患子茶的加工方法: CN104413175A[P]. 2015-03-18.
[42]	高媛, 贾黎明, 苏淑钗, 等. 无患子物候及开花结果特性[J]. 东北林业大学学报, 2015, 43(6):34-40, 123.
	GAO Y, JIA L M, SU S C, et al. Phenology and blossom-fruiting characteristics of Sapindus mukorossi[J]. J Northeast For Univ, 2015, 43(6):34-40, 123. DOI: 10.13759/j.cnki.dlxb.20150522.062. doi: 10.13759/j.cnki.dlxb.20150522.062

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器官 organs	L8	F1	B	R	P	SC	SK
L8	1.000
F1	-1.000^**/-0.566	1.000
B	0.790/-0.999^*	-0.795/0.532	1.000
R	0.563/-0.981	-0.569/0.716	0.951/0.972	1.000
P	0.097/-0.979	-0.090/0.723	-0.533/0.970	-0.768/1.000^**	1.000
SC	0.795/NA	-0.799/NA	1.000^**/NA	0.949/NA	-0.527/NA	1.000
SK	-0.986/NA	0.985/NA	-0.677/NA	-0.418/NA	-0.261/NA	-0.683/NA	1.000

Contents of the total saponins and total flavonoids in different organs of Sapindus mukorossi

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