Cloning and expression characteristics of PdMODD genes in Populus × euramericana

ZHANG Tengqian, ZHANG Weixi, DING Changjun, ZHANG Jing, HU Zanmin, SU Xiaohua

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (2) : 43-50.

PDF(4500 KB)
南京林业大学学报（自然科学版）
PDF(4500 KB)
JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (2) : 43-50. DOI: 10.12302/j.issn.1000-2006.202007015

Cloning and expression characteristics of PdMODD genes in Populus × euramericana

Author information +
History +

Abstract

【Objective】To explore the function of PdMODD in the growth and development of Populus × euramericana, we analyzed their sequence characteristics, subcellular localization and expression patterns in response to salt and drought stress, which will provide a theoretical basis for revealing the molecular mechanisms of stress resistance and breeding new poplar varieties. 【Method】Based on the rice MODD amino acid sequence, Podel.08G114100, Podel.10G158900 and Podel.17G098500 were selected using BLAST in the genome of Populus deltoides WV94 v2.1. Using these gene sequences as references and Populus × euramericana ‘Bofeng 3 hao’ cDNA as template, three genes (PdMODD1,PdMODD2 and PdMODD3) were cloned. The structural characteristics of the PdMODD protein and its genetic relationship with its homologous proteins were analyzed using bioinformatics methods. The subcellular localization of PdMODD genes was analyzed using particle bombardment. The expression characteristics of PdMODD genes in different tissues and their response to different stresses were analyzed using qRT-PCR. 【Results】The open reading frames of PDMODD1-PDMODD3 genes were 1 065, 1 065 and 1 074 bp, encoding 354, 354 and 357 amino acids, respectively, all of which were unstable hydrophilic proteins located in the nucleus.PdMODD genes are all members of the NINJA family and contain three conserved domains, one of which is the transcriptional repression motif EAR. Phylogenetic analysis demonstrated that PdMODD1 protein was closely related to PtAFP2 (Populus trichocarpa) and located in the same branch as AtAFP1 (Arabidopsis thaliana) and MeAFP2 (Manihot esculenta). PdMODD2 protein had a close relationship with JcAFP2 (Jatropha curcas) and converged into a branch with AtAFP2; PdMODD3 was closely related to PtAFP3-1 and located in the same branch as QsAFP3 (Quercus suber) and AtAFP4. The subcellular localization results showed that PdMODD genes were localized in the nucleus. The results of tissue specificity analysis showed that PdMODD genes were highly expressed in the leaves and the least expressed in the roots. The expression patterns of stress response showed that under NaCl and PEG stress, PdMODD1, PdMODD2 and PdMODD3 were significantly upregulated in the root, stem and leaf tissues. 【Conclusion】PdMODD1-PdMODD3 are all members of the NINJA family, with the conserved transcriptional repression motif EAR and tissue specificity. They were highly expressed in the leaves, significantly induced by NaCl and PEG stress, and were in a branch with AtAFP1, AtAFP2 and AtAFP4, which had negative regulatory effects on ABA and salt stress. It is hypothesized that PdMODD1, PdMODD2 and PdMODD3 may play an important regulatory role in response to P. euramericana ‘Bofeng 3 hao’ salt and drought stress.

Key words

Populus × euramaricana ‘Bofeng 3 hao’ / PdMODD / bioinformatics analysis / expression pattern analysis

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations
ZHANG Tengqian , ZHANG Weixi , DING Changjun , et al . Cloning and expression characteristics of PdMODD genes in Populus × euramericana[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2021, 45(2): 43-50 https://doi.org/10.12302/j.issn.1000-2006.202007015

References

List( Publishing order | Descend order by publishing year | Descend order by cited within ) Chart analysis
[1]
KAGALE S, ROZWADOWSKI K. EAR motif-mediated transcriptional repression in plants[J]. Epigenetics, 2011,6(2):141-146.DOI: 10.4161/epi.6.2.13627.
Cited in this article [2]
[2]
张健飞, 权瑞党, 黄荣峰. EAR转录抑制子结构及功能的研究[J]. 中国农业科技导报, 2011,13(4):53-57.
ZHANG J F, QUAN R D, HUANG R F. Studies on structure and function of repressors with EAR motif[J]. J Agric Sci Technol, 2011,13(4):53-57.DOI: 10.3969/j.issn.1008-0864.2011.04.08.
Cited in this article [2]
[3]
TANG N, MA S, ZONG W, et al. MODD mediates deactivation and degradation of OsbZIP46 to negatively regulate ABA signaling and drought resistance in rice[J]. Plant Cell, 2016,28(9):2161-2177.DOI: 10.1105/tpc.16.00171.
Cited in this article [4]
[4]
GARCIA M E, LYNCH T, PEETERS J, et al. A small plant-specific protein family of ABI five binding proteins (AFPs) regulates stress response in germinating Arabidopsis seeds and seedlings[J]. Plant Mol Biol, 2008,67(6):643-658.DOI: 10.1007/s11103-008-9344-2.
Cited in this article [4]
[5]
PAUWELS L, BARBERO G F, GEERINCK J, et al. NINJA connects the co-repressor TOPLESS to jasmonate signalling[J]. Nature, 2010,464(7289):788-791.DOI: 10.1038/nature08854.
Cited in this article [1]
[6]
KAZAN K, MANNERS J M. JAZ repressors and the orchestration of phytohormone crosstalk[J]. Trends Plant Sci, 2012,17(1):22-31.DOI: 10.1016/j.tplants.2011.10.006.
Cited in this article [1]
[7]
DE GEYTER N, GHOLAMI A, GOORMACHTIG S, et al. Transcriptional machineries in jasmonate-elicited plant secondary metabolism[J]. Trends Plant Sci, 2012,17(6):349-359.DOI: 10.1016/j.tplants.2012.03.001.
Cited in this article [1]
[8]
MA S, TANG N, LI X, et al. Reversible histone H2B monoubiquitination fine-tunes abscisic acid signaling and drought response in rice[J]. Mol Plant, 2019,12(2):263-277.DOI: 10.1016/j.molp.2018.12.005.
Cited in this article [1]
[9]
XIANG Y, TANG N, DU H, et al. Characterization of OsbZIP23 as a key player of the basic leucine zipper transcription factor family for conferring abscisic acid sensitivity and salinity and drought tolerance in rice[J]. Plant Physiol, 2008,148(4):1938-1952.DOI: 10.1104/pp.108.128199.
Cited in this article [1]
[10]
LU G J, GAO C X, ZHENG X N, et al. Identification of OsbZIP72 as a positive regulator of ABA response and drought tolerance in rice[J]. Planta, 2009,229(3):605-615.DOI: 10.1007/s00425-008-0857-3.
Cited in this article [1]
[11]
王倩姿, 王玉, 孙志梅, 等. 腐植酸类物质的施用对盐碱地的改良效果[J]. 应用生态学报, 2019,30(4):1227-1234.
WANG Q Z, WANG Y, SUN Z M, et al. Amelioration effect of humic acid on saline-alkali soil[J]. Chin J Appl Ecol, 2019,30(4):1227-1234.DOI: 10.13287/j.1001-9332.201904.001.
[12]
徐淑平, 卫志明. 基因枪的使用方法介绍[J]. 植物生理学通讯, 1998,34(1):41-43.
XU S P, WEI Z M. Introduction to method of microprojectile bombardment and its application[J]. Plant Physiol Commun, 1998,34(1):41-43.DOI: 10.13592/j.cnki.ppj.1998.01.014.
Cited in this article [1]
[13]
LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method[J]. Methods (San Diego Calif), 2001,25(4):402-408.DOI: 10.1006/meth.2001.1262.
Cited in this article [1]
[14]
刘中原, 姜波, 吕佳欣, 等. 刚毛柽柳Th2CysPrx基因的互作蛋白及其表达模式分析[J]. 南京林业大学学报(自然科学版), 2019,43(2):86-92.
LIU Z Y, JIANG B, LYU J X, et al. Interacting proteins of Tamarix hispida Th2CysPrx and their expression pattern analysis[J]. J Nanjing For Univ (Nat Sci Ed), 2019,43(2):86-92.DOI: 10.3969/j.issn.1000-2006.201806018.
Cited in this article [1]
[15]
杜娟, 柴友荣. 植物转录抑制子的结构特征及其作用机理[J]. 植物学通报, 2008,25(3):344-353.
DU J, CHAI Y R. Structural features and action mechanisms of plant transcriptional repressors[J]. Chin Bull Bot, 2008,25(3):344-353.DOI: 10.3969/j.issn.1674-3466.2008.03.012.
Cited in this article [1]
[16]
KAGALE S, LINKS M G, ROZWADOWSKI K. Genome-wide analysis of ethylene-responsive element binding factor-associated amphiphilic repression motif-containing transcriptional regulators in Arabidopsis[J]. Plant Physiol, 2010,152(3):1109-1134.DOI: 10.1104/pp.109.151704.
Cited in this article [1]
[17]
WEIGEL R R, PFITZNER U M, GATZ C. Interaction of NIMIN1 with NPR1 modulates PR gene expression in Arabidopsis[J]. Plant Cell, 2005,17(4):1279-1291.DOI: 10.1105/tpc.104.027441.
Cited in this article [1]
[18]
CHERN M, CANLAS P E, FITZGERALD H A, et al. Rice NRR,a negative regulator of disease resistance,interacts with Arabidopsis NPR1 and rice NH1[J]. Plant J, 2005,43(5):623-635.DOI: 10.1111/j.1365-313x.2005.02485.x.
Cited in this article [1]
[19]
OHTA M, MATSUI K, HIRATSU K, et al. Repression domains of class II ERF transcriptional repressors share an essential motif for active repression[J]. Plant Cell, 2001,13(8):1959-1968.DOI: 10.1105/tpc.010127.
Cited in this article [1]
[20]
LIU W, KAREMERA N J U, WU T, et al. The ethylene response factor AtERF4 negatively regulates the iron deficiency response in Arabidopsis thaliana[J]. PLoS One, 2017,12(10):e0186580.DOI: 10.1371/journal.pone.0186580.
Cited in this article [1]
[21]
YAISH M W, EL-KEREAMY A, ZHU T, et al. The APETALA-2-like transcription factor OsAP2-39 controls key interactions between abscisic acid and gibberellin in rice[J]. PLoS Genet, 2010,6(9):e1001098.DOI: 10.1371/journal.pgen.1001098.
Cited in this article [1]
[22]
WAN L, ZHANG J, ZHANG H, et al. Transcriptional activation of OsDERF1 in OsERF3 and OsAP2-39 negatively modulates ethylene synjournal and drought tolerance in rice[J]. PLoS One, 2011,6(9):e25216.DOI: 10.1371/journal.pone.0025216.
Cited in this article [1]
[23]
ZHANG H W, ZHANG J F, QUAN R D, et al. EAR motif mutation of rice OsERF3 alters the regulation of ethylene biosynjournal and drought tolerance[J]. Planta, 2013,237(6):1443-1451.DOI: 10.1007/s00425-013-1852-x.
Cited in this article [1]
[24]
PAN I C, LI C W, SU R C, et al. Ectopic expression of an EAR motif deletion mutant of SlERF3 enhances tolerance to salt stress and Ralstonia solanacearum in tomato[J]. Planta, 2010,232(5):1075-1086.DOI: 10.1007/s00425-010-1235-5.
Cited in this article [1]
[25]
HUANG J, YANG X, WANG M M, et al. A novel rice C2H2-type zinc finger protein lacking DLN-box/EAR-motif plays a role in salt tolerance[J]. Biochim et Biophys Acta (BBA)-Gene Struct Expr, 2007,1769(4):220-227.DOI: 10.1016/j.bbaexp.2007.02.006.
Cited in this article [1]
[26]
LOPEZ-MOLINA L, MONGRAND S, KINOSHITA N, et al. AFP is a novel negative regulator of ABA signaling that promotes ABI5 protein degradation[J]. Genes Dev, 2003,17(3):410-418.DOI: 10.1101/gad.1055803.
Cited in this article [2]
[27]
OHNISHI N, HIMI E, YAMASAKI Y, et al. Differential expression of three ABA-insensitive five binding protein (AFP)-like genes in wheat[J]. Genes Genet Syst, 2008,83(2):167-177.DOI: 10.1266/ggs.83.167.
Cited in this article [1]
[28]
WU J, SENG S, CARIANOPOL C, et al. Cloning and characterization of a novel Gladiolus hybridus AFP family gene (GhAFP-like) related to corm dormancy[J]. Biochem Biophys Res Commun, 2016,471(1):198-204.DOI: 10.1016/j.bbrc.2016.01.146.
Cited in this article [1]
[29]
HUANG M D, WU W L. Overexpression of TMAC2,a novel negative regulator of abscisic acid and salinity responses,has pleiotropic effects in Arabidopsis thaliana[J]. Plant Mol Biol, 2007,63(4):557-569.DOI: 10.1007/s11103-006-9109-8.
Cited in this article [1]
[30]
CAO M J, ZHANG Y L, LIU X, et al. Combining chemical and genetic approaches to increase drought resistance in plants[J]. Nat Commun, 2017,8(1):1183.DOI: 10.1038/s41467-017-01239-3.
Cited in this article [1]

RIGHTS & PERMISSIONS

Copyright reserved © 2021
PDF(4500 KB)

Accesses

Citation

Detail
Sections
Recommended
The full text is translated into English by AI, aiming to facilitate reading and comprehension. The core content is subject to the explanation in Chinese.

Author

Reader

Reviewer

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

/

〈 〉