Community structure of phoD phosphate solubilizing bacteria in rhizosphere soil of different blueberry cultivars

doi:10.12302/j.issn.1000-2006.202007050

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (2): 95-102.doi: 10.12302/j.issn.1000-2006.202007050

Community structure of phoD phosphate solubilizing bacteria in rhizosphere soil of different blueberry cultivars

WU Yejiao¹(), GAO Yuan¹, CAO Chengliang¹, JIANG Yuji³, LYU Lianfei², WU Wenlong², JIANG Jihong¹, ZHU Hong²^,^*(), LI Rongpeng¹^,^*()

1. Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou 221116, China
2. Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210018, China
3. State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China

Received:2020-07-05 Accepted:2021-04-29 Online:2022-03-30 Published:2022-04-08
Contact: ZHU Hong,LI Rongpeng E-mail:wyj512x@163.com;gogen@yeah.net;lirongpeng@jsnu.edu.cn

Abstract

Abstract:

【Objective】The objective was to study the effects of different rabbiteye blueberry (Vaccinium ashei Reade, RB) cultivars on the composition of phoD soil phosphate solubilizing bacteria. 【Method】 Using the high-throughput sequencing of phoD (encoding an alkaline phosphatase gene) in rhizosphere soils of 12 rabbiteye blueberry cultivars, the diversity and composition of soil phoD phosphate-solubilizing bacterial communities were analyzed, and interactions between rhizosphere phosphate solubilizing bacteria, blueberry cultivars, and soil properties were analyzed. 【Result】Among the 12 rabbiteye blueberry cultivars, ‘Centurion’ (RB4) showed the highest community diversity; the core rhizosphere phoD bacterial community comprised the α-proteobacteria rhizobiales and rhodospirillales, the β-proteobacteria burkholderiales, the γ-proteobacteria xanthomonadales, oceanospirillales, pseudomonadales, and the actinobacteria streptomycetales. The phoD bacterial communities in the blueberry rhizosphere can be divided into two blueberry groups, RBⅠ and RBⅡ, and the relative abundance of α-proteobacteria in the RBⅠ group was significantly higher. The soil total phosphorus (TP) and phoD bacterial community composition were the main influencing factors of AP. 【Conclusion】Different blueberry cultivars and soil properties had significant effects on the abundance of rhizosphere phoD phosphate-solubilizing bacteria, however, the community diversity did not differ significantly from that of the control. These results provide an effective data support for further elucidation of the symbiotic relationship between plants and phosphate-solubilizing bacteria in the rhizosphere under acidic soil conditions.

Key words: blueberry, rhizosphere, soil phosphate solubilizing bacteria, alkaline phosphatase gene (phoD), available P

CLC Number:

S663

WU Yejiao, GAO Yuan, CAO Chengliang, JIANG Yuji, LYU Lianfei, WU Wenlong, JIANG Jihong, ZHU Hong, LI Rongpeng. Community structure of phoD phosphate solubilizing bacteria in rhizosphere soil of different blueberry cultivars[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(2): 95-102.

Figures/Tables 6

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Community structure of phoD phosphate solubilizing bacteria in rhizosphere soil of different blueberry cultivars

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