Community structure of phoD phosphate solubilizing bacteria in rhizosphere soil of different blueberry cultivars

WU Yejiao, GAO Yuan, CAO Chengliang, JIANG Yuji, LYU Lianfei, WU Wenlong, JIANG Jihong, ZHU Hong, LI Rongpeng

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (2) : 95-102.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (2) : 95-102. DOI: 10.12302/j.issn.1000-2006.202007050

Community structure of phoD phosphate solubilizing bacteria in rhizosphere soil of different blueberry cultivars

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Abstract

【Objective】The objective was to study the effects of different rabbiteye blueberry (Vaccinium ashei Reade, RB) cultivars on the composition of phoD soil phosphate solubilizing bacteria. 【Method】 Using the high-throughput sequencing of phoD (encoding an alkaline phosphatase gene) in rhizosphere soils of 12 rabbiteye blueberry cultivars, the diversity and composition of soil phoD phosphate-solubilizing bacterial communities were analyzed, and interactions between rhizosphere phosphate solubilizing bacteria, blueberry cultivars, and soil properties were analyzed. 【Result】Among the 12 rabbiteye blueberry cultivars, ‘Centurion’ (RB4) showed the highest community diversity; the core rhizosphere phoD bacterial community comprised the α-proteobacteria rhizobiales and rhodospirillales, the β-proteobacteria burkholderiales, the γ-proteobacteria xanthomonadales, oceanospirillales, pseudomonadales, and the actinobacteria streptomycetales. The phoD bacterial communities in the blueberry rhizosphere can be divided into two blueberry groups, RBⅠ and RBⅡ, and the relative abundance of α-proteobacteria in the RBⅠ group was significantly higher. The soil total phosphorus (TP) and phoD bacterial community composition were the main influencing factors of AP. 【Conclusion】Different blueberry cultivars and soil properties had significant effects on the abundance of rhizosphere phoD phosphate-solubilizing bacteria, however, the community diversity did not differ significantly from that of the control. These results provide an effective data support for further elucidation of the symbiotic relationship between plants and phosphate-solubilizing bacteria in the rhizosphere under acidic soil conditions.

Key words

blueberry / rhizosphere / soil phosphate solubilizing bacteria / alkaline phosphatase gene (phoD) / available P

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WU Yejiao , GAO Yuan , CAO Chengliang , et al . Community structure of phoD phosphate solubilizing bacteria in rhizosphere soil of different blueberry cultivars[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2022, 46(2): 95-102 https://doi.org/10.12302/j.issn.1000-2006.202007050

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