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Effects of salicylic acid on germination of Cyclobalanopsis glauca seeds under NaHCO3 stress in Karst area of northwest Guangxi
DENG Ping, ZHAO Ying, WANG Xia, CHEN Qiuyou, WU Min
JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (4) : 114-122.
PDF(1888 KB)
PDF(1888 KB)
Effects of salicylic acid on germination of Cyclobalanopsis glauca seeds under NaHCO3 stress in Karst area of northwest Guangxi
【Objective】This study aimed to alleviate NaHCO3 stress in Cyclobalanopsis glauca by investigating the effects of exogenous application of salicylic acid (SA) on the seed germination and physiological characteristics, under bicarbonate stress. 【Method】Changes in germination rates and indexes, vigor indexes, salt tolerance indexes, relative salt damage rates, malondialdehyde (MDA) content, antioxidant superoxide dismutase (SOD), peroxidase (POD) and cata-lase (CAT) activities as well as osmotic adjustment substances agents (proline, soluble sugar and soluble protein) content were assessed in C. glauca seeds under NaHCO3 (0, 24, 48, 72, 96, 120 and 144 mmol/L) stress and exogenous application of salicylic acid (SA: 0 and 0.50 mmol/L) treatment. 【Result】①Germination parameters and salt tole-rance were lower, and salt damage rates were significantly higher under NaHCO3 stress, without SA, in C. glauca seeds compared to those of the control. However, seeds had higher germination parameters, a higher salt tolerance index, and lower relative salt damage rates under NaHCO3 stress with SA compared to those without SA. The seed germination parameters and a salt tolerance index were always higher under 0-72 mmol/L NaHCO3 stress with SA treatment compared with the control group. The germination parameters and a salt tolerance index were maximal, and the relative salt damage rate was minimal under 72 mmol/L NaHCO3 stress with 0.50 mmol/L of SA treatment. ②The POD and CAT activities of seeds without SA initially increased, then decreased with increasing NaHCO3 concentrations, and SOD activity was significantly lower than that of the control group, whereas the MDA content rapidly increased. Antioxidant enzyme activities and MDA contents were significantly higher and lower, respectively, under NaHCO3 stress with, than without SA. The activities of SOD and POD were maximal in 72 mmol/L of NaHCO3 with 0.50 mmol/L of SA. ③The contents of osmotic adjustment agents were high under NaHCO3 stress without SA, and increased under NaHCO3 stress with SA compared with the control. The contents of osmotic adjustment agents were maximal under 72 mmol/L of NaHCO3 with 0.50 mmol/L of SA.【Conclusion】 The effects of 72 mmol/L NaHCO3 stress on C. glauca seeds sowed and bred in the Karst areas were alleviated after immersion in 0.50 mmol/L exogenous SA.
Cyclobalanopsis glauca / seed germination / salicylic acid / NaHCO3 / Karst area
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