Structure and variation analyses of chloroplast genomes in Carpinus

doi:10.12302/j.issn.1000-2006.202009007

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (2): 25-34.doi: 10.12302/j.issn.1000-2006.202009007

Previous Articles Next Articles

Structure and variation analyses of chloroplast genomes in Carpinus

ZHAO Runan¹(), CHU Xiaojie², LIU Wei¹, HE Qianqian¹, ZHU Zunling¹^,³^,^*()

1. Co-Innovation Center for the Sustainable Forestry in Southern China, College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China
2. College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China
3. College of Art and Design, Nanjing Forestry University, Nanjing 210037, China

Received:2020-09-02 Accepted:2020-11-16 Online:2021-03-30 Published:2021-04-09
Contact: ZHU Zunling E-mail:zhao-rn@njfu.edu.cn;zhuzunling@njfu.edu.cn

Abstract

Abstract:

【Objective】Chloroplasts are the organelles responsible for the photosynthesis in green plants. The chloroplast genome structure and gene composition of higher plants are relatively conservative, but can show different degrees of variation due to hybridization, evolution and gene introgression. It contains a significant amount of DNA information, which is used in species classification, phylogeny and origin. Carpinus is one of the most evolved genus in the Betulaceae family. Although the chloroplast genomes of more than ten species of Carpinus have been sequenced and assembled, only a few of these have been fully studied. Therefore, this study aimed to understand the chloroplast genome gene composition and structural characteristics of Carpinus, reveal the contraction and expansion of the inverted repeat (IR) region boundary, and lay a foundation for further studies on phylogenetic relationships, species identification, genome evolution and resource utilization and provide a reference for the development of Carpinus DNA barcoding. 【Method】GeSeq was used to annotate the chloroplast genome with Ostrya rehdariana as the reference genome. Then, the number of genes, genomic DNA base composition (GC) content, large single copy (LSC), small single copy (SSC) and two IR regions of the chloroplast genomes of 16 Carpinus species were obtained. The structure, boundary contraction and expansion, and sequence variation were then compared by using OGDRAW, IRscope and mVISTA programs. The phylogenetic relationship of Carpinus was analyzed with Quercus acutissima as an outgroup. 【Result】We found that the chloroplast genomes of 16 species were circular, double-stranded, which were tetrads containing one LSC and SSC and two IRs. The difference in genome size was small, and the maximum difference was only 1 902 bp. The differences in IR region length and GC content among species were small. The gene sequence length and composition were relatively conservative, with the number of rRNAs (8) as the most conservative; however, there was significant diversity at the four boundaries. There were differences in the non-coding regions, and the degree of variation was high; however, the difference in gene coding region was small and highly conservative. Among the four parts of the chloroplast genome, the LSC region had the highest degree of variation, while the IRa region had the lowest. There were significant differences in the coding regions of psbA, rps16, atpA, rps19, ndhF, ndhI and ycf1 genes. In addition, the non-coding regions of the intergenic regions of ycf3-trnS, trnS-rps4, trnH-psbA, psbZ-trnfM, matK-rps16, rps16-trnQ, trnQ-psbK, ccsA-ndhD, accD-psaI, ndhC-trnV, trnT-trnL, trnF-ndhJ, atpB-rbcL, trnT-psbD, trnE-trnT, trnD-trnY, and rpl32-trnL were significantly different. In addition, the coding region length of most genes was very conservative, and the variation of coding gene length of proteins containing introns originated from the change in intron length or coding region length. The phylogenetic tree supported a division of Carpinus and Distegocarpus sections. In addition, due to the significant geographical isolation,C. caroliniana and C. betulus showed a relatively distant phylogenetic relationship with other species of Carpinus. 【Conclusion】The chloroplast genome of Carpinus was highly conservative with little gene sequence differences; no large-scale inversion or gene rearrangement was detected. However, the IRs and SC boundaries had significant diversity. In addition, some genes with significant differences in coding or non-coding regions may provide a reference for developing new DNA barcodes of Carpinus species.

Key words: Carpinus, chloroplast genome, variation, evolution, phylogeny

CLC Number:

Q941

ZHAO Runan, CHU Xiaojie, LIU Wei, HE Qianqian, ZHU Zunling. Structure and variation analyses of chloroplast genomes in Carpinus[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(2): 25-34.

Figures/Tables 7

Table 1

Fig.1

Table 2

Fig.2

Fig.3

Table 3

Length of the coding region and the intron-containing gene in chloroplast genome of 16 species in Carpinusbp"

树种 species	rps16		atpF		rpoC1		ycf3		clpP		petB		petD		rpl16		rpl2		ndhB		ndhA
树种 species	编码区长 coding region length	基因全长 gene length	编码区长 coding region length	基因全长 gene length	编码区长 coding region length	基因全长 gene length	编码区长 coding region length	基因全长 gene length	编码区长 coding region length	基因全长 gene length	编码区长 coding region length	基因全长 gene length	编码区长 coding region length	基因全长 gene length	编码区长 coding region length	基因全长 gene length	编码区长 coding region length	基因全长 gene length	编码区长 coding region length	基因全长 gene length	编码区长 coding region length	基因全长 gene length
川黔千金榆 C. fangiana	258	1 132	555	1 304	2 058	2 871	507	2 002	591	2 014	648	1 372	483	1 103	408	1 481	825	1 513	1 539	2 225	1 095	2 292
千金榆 C. cordata	258	1 132	555	1 304	2 058	2 885	507	2 002	591	2 015	648	1 441	483	1 103	408	1 498	825	1 513	1 539	2 225	1 095	2 293
天台鹅耳枥 C. tientaiensis	258	1 132	555	1 305	2 058	2 870	507	2 008	591	2 013	-	-	483	1 103	408	1 484	825	1 513	1 539	2 225	1 095	2 292
普陀鹅耳枥 C. putoensis	258	1 132	555	1 306	2 058	2 869	507	2 008	591	2 015	-	-	483	1 103	408	1 316	825	1 513	1 539	2 225	1 095	2 287
太鲁阁鹅耳枥 C. hebestroma	240	1 132	558	1 307	2 052	2 879	510	2 002	591	2 013	648	1 445	483	1 103	408	1 483	864	1 513	1 539	2 225	1 092	2 283
紫脉鹅耳枥 C. purpurinervis	240	1 132	558	1 303	2 052	2 869	510	2 007	591	2 014	648	1 441	483	1 103	408	1 483	864	1 513	1 539	2 225	1 092	2 292
宝华鹅耳枥 C. oblongifolia	240	1 132	558	1 306	2 052	2 870	510	2 002	591	2 012	648	1 445	483	1 103	408	1 483	864	1 513	1 539	2 225	1 092	2 291
岩生鹅耳枥 C. rupestris	258	1 132	555	1 304	2 058	2 869	507	2 008	591	2 013	648	1 441	483	1 103	408	1 483	825	1 513	1 539	2 225	1 095	2 292
多脉鹅耳枥 C. polyneura	258	1 132	555	1 306	2 058	2 870	507	1 995	591	2 012	648	1 445	483	1 103	408	1 483	825	1 513	1 539	2 225	1 095	2 290
云南鹅耳枥 C. monbeigiana	258	1 132	555	1 305	2 058	2 870	507	2 002	591	2 011	648	1 445	483	1 103	408	1 483	825	1 513	1 539	2 225	1 095	2 298
昌化鹅耳枥 C. tschonoskii	240	1 132	558	1 307	2 052	2 871	510	2 002	591	2 012	648	1 445	483	1 103	408	1 483	864	1 513	1 539	2 225	1 092	2 289
川陕鹅耳枥 C. fargesiana	240	1 132	558	1 305	2 052	2 870	510	2 000	591	2 011	648	1 445	483	1 103	408	1 483	864	1 513	1 539	2 225	1 092	2 291
美洲鹅耳枥 C. caroliniana	240	1 132	558	1 308	2 052	2 871	510	2 001	591	2 010	648	1 441	483	1 103	408	1 489	864	1 513	1 539	2 225	1 092	2 303
欧洲鹅耳枥 C. betulus	240	1 132	558	1 306	2 052	2 871	510	2 001	591	2 015	648	1 449	483	1 103	408	1 478	864	1 513	1 539	2 225	1 092	2 289
疏花鹅耳枥 C. laxiflora	240	1 132	558	1 308	2 052	2 870	507	2 002	591	2 009	648	1 445	483	1 103	411	1 483	864	1 513	1 539	2 225	1 092	2 302
雷公鹅耳枥 C. viminea	240	1 132	558	1 304	2 052	2 869	510	2 008	591	2 013	648	1 441	483	1 103	408	1 483	864	1 513	1 539	2 225	1 092	2 293

Table 3

Fig.4

References 35

[1]	NEUHAUS H E, EMES M J. Nonphotosynthetic metabolism in plastids[J]. Annu Rev Plant Physiol Plant Mol Biol, 2000,51(1):111-140.DOI: 10.1146/annurev.arplant.51.1.111.
[2]	SHINOZAKI K, OHME M, TANAKA M, et al. The complete nucleotide sequence of the tobacco chloroplast genome[J]. Plant Mol Biol Report, 1986,4(3):111-148.DOI: 10.1007/BF02669253.
[3]	NOCK C J, WATERS D L, EDWARDS M A, et al. Chloroplast genome sequences from total DNA for plant identification[J]. Plant Biotechnol J, 2011,9(3):328-333.DOI: 10.1111/j.1467-7652.2010.00558.x.
[4]	RAVI V, KHURANA J P, TYAGI A K, et al. An update on chloroplast genomes[J]. Plant Syst Evol, 2008,271(1/2):101-122.DOI: 10.1007/s00606-007-0608-0.
[5]	HU Y, ZHANG Q, RAO G, et al. Occurrence of plastids in the sperm cells of Caprifoliaceae:biparental plastid inheritance in angiosperms is unilaterally derived from maternal inheritance[J]. Plant Cell Physiol, 2008,49(6):958-968.DOI: 10.1093/pcp/pcn069.
[6]	ZHANG Q SODMERGE N. Why does biparental plastid inheritance revive in angiosperms?[J]. J Plant Res, 2010,123(2):201-206.DOI: 10.1007/s10265-009-0291-z.
[7]	陈之端. 桦木科植物的系统发育和地理分布(续)[J]. 植物分类学报, 1994,32(2):101-153.
	CHEN Z D. Phylogeny and phytogeography of the Betulaceae (cont.)[J]. Acta Phytotaxon Sin, 1994,32(2):101-153.
[8]	李沛群, 郑斯绪. 中国植物志:第21卷[M]. 北京: 科学出版社, 1979: 84-85.
	LI P Q, ZHENG S X. Flora republicae popularis sinica: Vol. 21[M]. Beijing: Science Press, 1979: 84-85.
[9]	LI P C, SKVORTSOV A K. Flora of China: Vol. 4[M]. Beijing: Science Press, 1999: 289-300.
[10]	李素梅, 汪庆, 王淑安, 等. 江苏宝华山宝华鹅耳枥种群现状分析[J]. 植物资源与环境学报, 2020,29(1):52-58.
	LI S M, WANG Q, WANG S A, et al. Analysis on population status of Carpinus oblongifolia in Baohua Mountain of Jiangsu Province[J]. J Plant Resour Environ, 2020,29(1), 52-58 DOI: 10.3969/j.issn.1674-7895.2020.01.07.
[11]	FENG S, XIE X Y, WANG M C, et al. Characterization of the complete chloroplast genome of Carpinus putoensis[J]. Conserv Genet Resour, 2017,9(1):127-129.DOI: 10.1007/s12686-016-0604-1.
[12]	YANG Y Z, WANG M C, LU Z Q, et al. Characterization of the complete chloroplast genome of Carpinus tientaiensis[J]. Conserv Genet Resour, 2017,9(2):339-341.DOI: 10.1007/s12686-016-0668-y.
[13]	WANG G N, LI Y. The complete chloroplast genome of Carpinus hebestroma,a critically endangered species endemic to Taiwan[J]. Mitochondrial DNA Part B, 2018,3(2):693-694.DOI: 10.1080/23802359.2018.1481784.
[14]	WANG J R, WANG M H. Complete chloroplast genome sequence of Carpinus oblongifolia (Betulaceae) and phylogenetic analysis[J]. Mitochondrial DNA Part B, 2019,4(1):1304-1305.DOI: 10.1080/23802359.2019.1591216.
[15]	LEE M W, KIM S C, AHN J Y, et al. The complete chloroplast genome of Carpinus laxiflora (Betulaceae)[J]. Mitochondrial DNA Part B, 2019,4(1):1643-1644.DOI: 10.1080/23802359.2019.1604184.
[16]	LI Y, YANG Y Z, YU L, et al. Plastomes of nine hornbeams and phylogenetic implications[J]. Ecol Evol, 2018,8(17):8770-8778.DOI: 10.1002/ece3.4414.
[17]	杨霄月. 桦木科叶绿体基因组的系统发育分析[D]. 兰州:兰州大学, 2019.
	YANG X Y. Phylogenetic analysis of Betulaceae plastomes[D]. Lanzhou:Lanzhou University, 2019.
[18]	YANG X Y, WANG Z F, LUO W C, et al. Plastomes of Betulaceae and phylogenetic implications[J]. J Syst Evol, 2019,57(5):508-518.DOI: 10.1111/jse.12479.
[19]	GREINER S, LEHWARK P, BOCK R. OrganellarGenomeDRAW (OGDRAW) version 1.3.1:Expanded toolkit for the graphical visualization of organellar genomes[J]. Nucleic Acids Res, 2019,47(W1):W59-W64.DOI: 10.1093/nar/gkz238.
[20]	AMIRYOUSEFI A, HYVÖNEN J, POCZAI P. IRscope:an online program to visualize the junction sites of chloroplast genomes[J]. Bioinformatics, 2018,34(17):3030-3031.DOI: 10.1093/bioinformatics/bty220.
[21]	FRAZER K A, PACHTER L, POLIAKOV A, et al. VISTA:computational tools for comparative genomics[J]. Nucleic Acids Res, 2004,32(suppl_2):W273-W279.DOI: 10.1093/nar/gkh458.
[22]	KUMAR S, STECHER G, LI M, et al. MEGA X:Molecular evolutionary genetics analysis across computing platforms[J]. Mol Biol Evol, 2018,35(6):1547-1549.DOI: 10.1093/molbev/msy096.
[23]	CHUMLEY T W, PALMER J D, MOWER J P, et al. The complete chloroplast genome sequence of Pelargonium × hortorum:organization and evolution of the largest and most highly rearranged chloroplast genome of land plants[J]. Mol Biol Evol, 2006,23(11):2175-2190.DOI: 10.1093/molbev/msl089.
[24]	SERRANO M, WANG B, ARYAL B, et al. Export of salicylic acid from the chloroplast requires the multidrug and toxin extrusion-like transporter EDS5[J]. Plant Physiol, 2013,162(4):1815-1821.DOI: 10.1104/pp.113.218156.
[25]	GUISINGER M M, KUEHL J V, BOORE J L, et al. Extreme reconfiguration of plastid genomes in the angiosperm family Geraniaceae:rearrangements,repeats,and codon usage[J]. Mol Biol Evol, 2011,28(1):583-600.DOI: 10.1093/molbev/msq229.
[26]	HIRAO T, WATANABE A, KURITA M, et al. Complete nucleotide sequence of the Cryptomeria japonica D.Don.chloroplast genome and comparative chloroplast genomics: diversified genomic structure of coniferous species[J]. BMC Plant Biol, 2008,8(1):70.DOI: 10.1186/1471-2229-8-70.
[27]	PALMER J D, THOMPSON W F. Rearrangements in the chloroplast genomes of mung bean and pea[J]. PNAS, 1981,78(9):5533-5537.DOI: 10.1073/pnas.78.9.5533.
[28]	MAIER R M, NECKERMANN K, IGLOI G L, et al. Complete sequence of the maize chloroplast genome: gene content,hotspots of divergence and fine tuning of genetic information by transcript editing[J]. J Mol Biol, 1995,251(5):614-628.DOI: 10.1006/jmbi.1995.0460.
[29]	DEMPEWOLF H, KANE N C, OSTEVIK K L, et al. Establishing genomic tools and resources for Guizotia abyssinica (L.f.) Cass: the development of a library of expressed sequence tags,microsatellite loci,and the sequencing of its chloroplast genome[J]. Mol Ecol Resour, 2010,10(6):1048-1058.DOI: 10.1111/j.1755-0998.2010.02859.x.
[30]	童毅华, 彭权森, 夏念和. 香港桦木科一新种:香港鹅耳枥[J]. 热带亚热带植物学报, 2014(2):121-124.
	TONG Y H, PANG Q S, XIA N H. Carpinus insularis(Betulaceae): a new species from Hong Kong,China[J]. J Trop Subtrop Bot, 2014(2):121-124.DOI: 10.3969/j.issn.1005-3395.2014.02.002.
[31]	LU Z Q, LIU S Y, YANG X Y, et al. Carpinus langaoensis (Betulaceae),a new hornbeam species from the Daba Mountains in Shaanxi,China[J]. Phytotaxa, 2017,295(2):185.DOI: 10.11646/phytotaxa.295.2.6.
[32]	LU Z, LI Y, YANG X, et al. Carpinus tibetana(Betulaceae): a new species from southeast Tibet,China[J]. PhytoKeys, 2018(98):1-13.DOI: 10.3897/phytokeys.98.23639.
[33]	LU Z. Carpinus gigabracteatus: a new species from Southeast Yunnan,China[J]. PhytoKeys, 2020,145:47-56.DOI: 10.3897/phytokeys.145.49488.
[34]	CHEN Z D, MANCHESTER S R, SUN H Y. Phylogeny and evolution of the Betulaceae as inferred from DNA sequences,morphology,and paleobotany[J] . Am J Bot, 1999,86(8):1168-1181.DOI: 10.2307/2656981.
[35]	鲁志强. 中国桦木科榛亚科的物种界定研究[D]. 兰州:兰州大学, 2017.
	LU Z Q. Species delimitation in the subfamily coryloideae of Betulaceae in China[D]. Lanzhou:Lanzhou University, 2017.

Metrics

Comments

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

种名 species	GenBank登录号 GenBank No.	种名 species	GenBank登录号 GenBank No.
川黔千金榆C. fangiana	MG386371.1	多脉鹅耳枥C. polyneura	NC_039998.1
千金榆C. cordata	NC_036723.1	云南鹅耳枥C. monbeigiana	NC_039997.1
天台鹅耳枥C. tientaiensis	NC_034910.1	昌化鹅耳枥C. tschonoskii	NC_039938.1
普陀鹅耳枥C. putoensis	NC_033503.1	川陕鹅耳枥C. fargesiana	NC_039937.1
太鲁阁鹅耳枥C. hebestroma	NC_038131.1	美洲鹅耳枥C. caroliniana	NC_039935.1
紫脉鹅耳枥C. purpurinervis	NC_038093.1	欧洲鹅耳枥C. betulus	NC_039934.1
宝华鹅耳枥C. oblongifolia	MG720817.1	疏花鹅耳枥C. laxiflora	MK425701.1
岩生鹅耳枥C. rupestris	NC_039999.1	雷公鹅耳枥C. viminea	NC_039939.1

树种 speices	基因组大小/bp genome size	GC含量/% GC content	rRNA	tRNA	蛋白编码基因数 protein coding gene	总基因数 total genes	LSS/ bp	SSC/ bp	IR_a/bp	IR_b/bp
川黔千金榆 C. fangiana	158 787	36.50	8	31	86	126	88 168	18 537	26 041	26 041
千金榆 C. cordata	159 157	36.50	8	30	86	125	88 525	18 556	26 038	26 038
天台鹅耳枥 C. tientaiensis	160 104	36.40	8	29	86	124	89 445	18 588	26 035	26 036
普陀鹅耳枥 C. putoensis	159 673	36.40	8	29	85	122	89 019	18 565	26 043	26 046
太鲁阁鹅耳枥 C. hebestroma	159 231	36.50	8	30	85	123	88 168	18 872	26 095	26 096
紫脉鹅耳枥 C. purpurinervis	159 145	36.50	8	29	86	123	88 186	18 800	26 079	26 079
宝华鹅耳枥 C. oblongifolia	159 086	36.50	8	30	86	124	88 190	18 763	26 066	26 067
岩生鹅耳枥 C. rupestris	158 888	36.40	8	29	86	123	87 972	18 779	26 068	26 069
多脉鹅耳枥 C. polyneura	159 400	36.40	8	30	86	124	88 516	18 764	26 060	26 060
云南鹅耳枥 C. monbeigiana	159 450	36.40	8	30	86	124	88 553	18 775	26 061	26 061
昌化鹅耳枥 C. tschonoskii	159 505	36.40	8	30	86	124	88 616	18 758	26 065	26 066
川陕鹅耳枥 C. fargesiana	159 484	36.40	8	30	86	124	88 519	18 814	26 075	26 076
美洲鹅耳枥 C. caroliniana	160 151	36.30	8	31	86	125	88 555	18 517	26 542	26 543
欧洲鹅耳枥 C. betulus	160 583	36.40	8	29	86	123	88 282	17 184	27 567	27 568
疏花鹅耳枥 C. laxiflora	159 225	36.50	8	30	86	124	88 359	18 764	26 066	26 066
雷公鹅耳枥 C. viminea	158 681	36.50	8	29	86	123	87 808	18 808	26 032	26 033

Structure and variation analyses of chloroplast genomes in Carpinus

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 7

References 35

Related Articles 15

Recommended Articles

Metrics

Comments

Author

Reader

Reviewer

[1]	LIU Jie, ZHANG Lang, ZHANG Qingping. The evolution and driving mechanism of urban green space system: a case study of Xuchang City, Henan Province, China [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(3): 275-284.
[2]	YI Xiangui, LI Meng, WANG Xianrong. A review on the taxonomy study of Prunus subgen. Cerasus (Mill) A. Gray [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(3): 46-57.
[3]	LU Xudong, DONG Yuran, LI Yao, MAO Lingfeng. Community assembly mechanism for different planting ages of Chinese fir artificial forests in subtropical China [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(1): 67-73.
[4]	OU Yang, OUYANG Fangqun, SUN Meng, WANG Chao, WANG Junhui, AN Sanping, WANG Lifang, XU Na, WANG Meng. Young growth rhythm, annual and density interaction effects and selection strategies of Picea abies clones [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(6): 95-104.
[5]	FAN Boqing, LIU Dongyun, WANG Siyuan, Muhammad·Amir·Siddique . Spatio-temporal evolution of surface temperature in urban green space: a case study within the Sixth Ring Road in Beijing [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(5): 197-204.
[6]	CAO Linqing, ZHONG Qiuping, ZOU Yuling, TIAN Feng, HE Yichang. Leaf structure variations and relationship with environmental factors among germplasm resources of Vernicia fordii [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(4): 95-102.
[7]	CUI Zhihua, LYU Yanjie, YANG Xinyu. Analysis on the gradient evolution of soundscape between urban and rural areas in Nanjing Metropolitan area [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(3): 199-206.
[8]	WANG Wenyue, ZHANG Zhen, JIN Guoqing, SUN Linshan, QIU Yongbin, ZHOU Zhichun, YANG Tao. Family variation and selection of growth traits of eight-year-old Cupressus funebris in two sites [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(2): 42-48.
[9]	LIU Yamei, LIU Shengquan, ZHOU Liang, HU Jianjun, ZHAO Zicheng, ZHENG Xiangli. Anatomical characteristics and radial variations in eight poplar clones/cultivars [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(1): 234-240.
[10]	LUO Jianxun, LIU Furong, SONG Peng, LAI Shihui. Cryptomeria japonica ‘Fupang’: a new cultivar of C. japonica [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(1): 241-242.
[11]	CHU Chenchen, SUN Mingsheng, WU Yuhan, YAN Zhenyu, LI Ting, FENG Yangfan, GUO Ying, YIN Tongming, XUE Liangjiao. Pan-genome and genomic variation analyses of Populus [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(6): 251-260.
[12]	HE Xudong, SUI Dezong, WANG Hongling, HUANG Ruifang, ZHENG Jiwei, WANG Baosong. Research progresses of willow genetic breeding in China [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(6): 51-63.
[13]	JIA Qingbin, LIU Geng, ZHAO Jiali, LI Kuiyou, SUN Wensheng. Variation analyses of growth traits in half-sib families of Korean pine and superior families selection [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(4): 109-116.
[14]	WANG Bixia, DU Xiaoqi, DENG Yan, CAI Xiaomei, SU Guangcan. Seasonal variations of the nutrients and phenols from olive leaves in main cultivation varieties at Liangshan, Sichuan Province [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(4): 169-176.
[15]	SONG Shuang, SHI Mengxi, HU Shanshan, WANG Shaohan, XU Dawei. Evolutions and driving mechanisms of urban blue-green spaces in northeast China: a case study with the urban central district of Harbin City [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(4): 221-229.