Study on tissue culture and rapid propagation of Acer truncatum Bunge

doi:10.12302/j.issn.1000-2006.202009049

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (2): 220-224.doi: 10.12302/j.issn.1000-2006.202009049

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Study on tissue culture and rapid propagation of Acer truncatum Bunge

MA Qiuyue¹(), LI Qianzhong¹, LI Shushun¹, ZHU Lu¹, YAN Kunyuan¹, LI Shuxian², ZHANG Bin², WEN Jing¹^,^*()

1. Institute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
2. Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China

Received:2020-09-22 Accepted:2020-11-22 Online:2021-03-30 Published:2021-04-09
Contact: WEN Jing E-mail:yue.870808@163.com;jingwen@jaas.ac.cn

Abstract

Abstract:

【Objective】To establish a rapid propagation system, disinfection method and optimal medium were screened, which will lay the foundation for research on the breeding of Acer truncatum seeding. 【Method】The effects of different sterilization treatment mehods, basic media (1/2 MS, MS, 1/2 NN69, NN69), and plant hormones (IBA) on axillary buds and rooting were investigated using stems with axillary buds of A. truncatum. 【Result】When the stem with axillary buds was sterilized with 75% (volume fraction) alcohol for 30 s and 0.1% (mass fraction) HgCl₂ for 180 s, the survival rate was the highest. The NN69 medium supplemented with 0.2 mg/L IBA showed the highest axillary bud induction rate (97.2%) with the shortest time (10-15 d). The 1/2 NN69 medium supplemented with 0.4 mg/L IBA was suitable for plantlet rooting, with a rooting rate of 87.8%. 【Conclusion】The optimal sterilization method for stem segments of A. truncatum with axillary buds was 75% alcohol for 30 s and 0.1% HgCl₂ for 180 s. The optimal medium for axillary bud induction was NN69 with 0.2 mg/L IBA, and the optimal rooting medium was 1/2 NN69 with 0.4 mg/L IBA. The successful cultivation substrate was prepared by mixing peat, humus,and roseite at a volume ratio of 7:2:1, and the survival rate was 95.0% when the plantlets were moved to the nursery. The results of this study can provide a basis for large-scale propagation of A. truncatum.

Key words: Acer truncatum, tissue culture, rooting, rapid propagation

CLC Number:

S718

MA Qiuyue, LI Qianzhong, LI Shushun, ZHU Lu, YAN Kunyuan, LI Shuxian, ZHANG Bin, WEN Jing. Study on tissue culture and rapid propagation of Acer truncatum Bunge[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(2): 220-224.

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References 24

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处理 treatment	处理时间/s treatment time		污染率/% pollution ratio	死亡率/% death ratio	成活率/% survival ratio
处理 treatment	75% C₂H₅OH	0.1% HgCl₂	污染率/% pollution ratio	死亡率/% death ratio	成活率/% survival ratio
1	10	120	93.3±0.0 aA	2.6±0.5 dCD	4.1±0.5 ff
2	10	180	80.7±1.7 bB	3.3±0.0 bcdBC	15.9±1.7 eE
3	10	240	56.7±0.0 cC	2.6±0.5 dCD	40.7±0.5 dD
4	20	120	60.0±2.2 cC	3.0±0.5 cdBC	37.0±2.5 dD
5	20	180	45.6±1.5 dD	3.3±0.0 bcdBC	51.1±1.5 cC
6	20	240	37.8±1.5 eE	3.7±0.5 bcdBC	58.5±1.2 bB
7	30	120	44.4±3.0 dD	4.1±0.5 bcBC	51.5±2.7 cC
8	30	180	11.7±0.5 ff	4.4±0.0 bB	83.9±0.5 aA
9	30	240	9.3±0.5 ff	28.9±0.7 aA	61.9±1.2 bB

处理 treatment	采集时期 collected periods	采集地 collected plot	污染率/% pollution ratio	死亡率/% death ratio	成活率/% survival ratio
1	5月	室外大田	8.9±1.5 eD	1.5±0.5 bA	89.6±0.7 aA
2	5月	温室盆栽	7.8±1.5 eD	1.9±0.5 abA	90.4±0.8 aA
3	7月	室外大田	25.6±1.5 cB	3.3±0.7 aA	71.1±0.7 cC
4	7月	温室盆栽	18.9±0.3 dC	3.0±0.5 abA	78.1±0.9 bB
5	9月	室外大田	94.4±1.5 aA	2.2±0.7 abA	3.3±0.5 dD
6	9月	温室盆栽	31.1±1.5 bB	3.0±0.5 abA	65.9±3.5 eE

处理 treatment	培养基 culture mediums	IBA质量浓度/ (mg·L^-1) IBA mass content	出芽率/% induction ratio	腋芽数量 (>0.5 cm) cluster bud number	腋芽展叶时间/d axillary bud spreading time
1	1/2 MS	0	77.8±3.7 deG	1.5	30~35
2	1/2 MS	0.2	86.7±1.1 cBCDE	1.6	25~30
3	1/2 MS	0.4	85.4±0.7 cDEF	1.7	25~30
4	MS	0	75.6±0.7 eG	1.4	30~35
5	MS	0.2	92.2±0.7 bAB	1.8	25~30
6	MS	0.4	87.8±0.7 cBCD	1.7	25~30
7	NN69	0	88.3±0.0 bcABC	1.7	25~30
8	NN69	0.2	97.2±1.9 aA	1.9	10~15
9	NN69	0.4	93.9±0.7 abAB	1.9	10~15
10	1/2 NN69	0	81.1±4.1 cdFG	1.6	25~30
11	1/2 NN69	0.2	92.2±0.7 bAB	1.8	20~25
12	1/2 NN69	0.4	92.8±0.7 bAB	1.8	20~25

处理 treatment	培养基 culture medium	IBA/ (mg·L^-1)	生根率/% rooting ratio	根数/条 root number	根系生长状况 status of roots
1	1/2MS	0.2	47.8±1.5 deC	2.1±0.1 fE	长势稍差
2	1/2MS	0.4	66.7±1.5 deC	3.5±0.1 cC	长势一般
3	1/2MS	0.6	50.4±0.5 dC	2.1±0.1 ff	长势稍差
4	MS	0.2	41.9±2.7 efDE	2.1±0.2 ff	长势稍差
5	MS	0.4	50.0±0.7 dC	3.2±0.2 eCD	长势一般
6	MS	0.6	44.8±0.5 eCD	3.5±0.0 cdC	长势一般
7	NN69	0.2	33.3±4.4 gF	1.7±0.0 gF	长势稍差
8	NN69	0.4	47.4±1.0 deC	2.3±0.0 fE	长势稍差
9	NN69	0.6	36.7±0.7 gEF	1.6±0.1 gf	长势稍差
10	1/2NN69	0.2	70.7±1.2 bB	4.3±0.2 bB	长势最好
11	1/2NN69	0.4	87.8±0.7 aA	5.2±0.1 aA	长势最好
12	1/2NN69	0.6	65.9±0.5 cB	3.8±0.2 cC	长势稍差

Study on tissue culture and rapid propagation of Acer truncatum Bunge

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