Study on tissue culture and rapid propagation of Acer truncatum Bunge

doi:10.12302/j.issn.1000-2006.202009049

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (2): 220-224.doi: 10.12302/j.issn.1000-2006.202009049

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Study on tissue culture and rapid propagation of Acer truncatum Bunge

MA Qiuyue¹(), LI Qianzhong¹, LI Shushun¹, ZHU Lu¹, YAN Kunyuan¹, LI Shuxian², ZHANG Bin², WEN Jing¹^,^*()

1. Institute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
2. Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China

Received:2020-09-22 Accepted:2020-11-22 Online:2021-03-30 Published:2021-04-09
Contact: WEN Jing E-mail:yue.870808@163.com;jingwen@jaas.ac.cn

Abstract

Abstract:

【Objective】To establish a rapid propagation system, disinfection method and optimal medium were screened, which will lay the foundation for research on the breeding of Acer truncatum seeding. 【Method】The effects of different sterilization treatment mehods, basic media (1/2 MS, MS, 1/2 NN69, NN69), and plant hormones (IBA) on axillary buds and rooting were investigated using stems with axillary buds of A. truncatum. 【Result】When the stem with axillary buds was sterilized with 75% (volume fraction) alcohol for 30 s and 0.1% (mass fraction) HgCl₂ for 180 s, the survival rate was the highest. The NN69 medium supplemented with 0.2 mg/L IBA showed the highest axillary bud induction rate (97.2%) with the shortest time (10-15 d). The 1/2 NN69 medium supplemented with 0.4 mg/L IBA was suitable for plantlet rooting, with a rooting rate of 87.8%. 【Conclusion】The optimal sterilization method for stem segments of A. truncatum with axillary buds was 75% alcohol for 30 s and 0.1% HgCl₂ for 180 s. The optimal medium for axillary bud induction was NN69 with 0.2 mg/L IBA, and the optimal rooting medium was 1/2 NN69 with 0.4 mg/L IBA. The successful cultivation substrate was prepared by mixing peat, humus,and roseite at a volume ratio of 7:2:1, and the survival rate was 95.0% when the plantlets were moved to the nursery. The results of this study can provide a basis for large-scale propagation of A. truncatum.

Key words: Acer truncatum, tissue culture, rooting, rapid propagation

CLC Number:

S718

MA Qiuyue, LI Qianzhong, LI Shushun, ZHU Lu, YAN Kunyuan, LI Shuxian, ZHANG Bin, WEN Jing. Study on tissue culture and rapid propagation of Acer truncatum Bunge[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(2): 220-224.

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References 24

[1]	任红剑, 丰震, 乔谦, 等. 元宝枫叶片形态特征的地理变异[J]. 西北林学院学报, 2018,33(1):113-119.
	REN H J, FENG Z, QIAO Q, et al. Geographic variation trend of leaf morphology in Acer truncatum[J]. J Northwest For Univ, 2018,33(1):113-119.DOI: 10.3969/j.issn.1001-7461.2018.01.18.
[2]	MA Q Y, SUN T L, LI S S, et al. The Acer truncatum genome provides insights into nervonic acid biosynjournal[J]. Plant J, 2020,104(3):662-678.DOI: 10.1111/tpj.14954.
[3]	黄相中, 李聪, 古昆, 等. 元宝枫叶提取物的稳定性及抗氧化活性研究[J]. 云南大学学报(自然科学版), 2003,25(5):442-445,448.
	HUANG X Z, LI C, GU K, et al. Studies on antioxidative activity and stability of the extracts of Acer truncatum leave[J]. J Yunnan Univ (Nat Sci Ed), 2003,25(5):442-445,448.DOI: 10.3321/j.issn:0258-7971.2003.05.017.
[4]	王性炎, 樊金栓, 王姝清. 中国含神经酸植物开发利用研究[J]. 中国油脂, 2006,31(3):69-71.
	WANG X Y, FAN J S, WANG S Q. Development situation and outlook of nervonic acid plants in China[J]. China Oils Fats, 2006,31(3):69-71.DOI: 10.3321/j.issn:1003-7969.2006.03.025.
[5]	LI Q, CHEN J, YU X, et al. A mini review of nervonic acid:source,production,and biological functions[J]. Food Chem, 2019,301:125286. DOI: 10.1016/j.foodchem.2019.125286.
[6]	MEDEMA S, MOCKING R J T, KOETER M W J, et al. Levels of red blood cell fatty acids in patients with psychosis,their unaffected siblings,and healthy controls[J]. Schizophr Bull, 2016,42(2):358-368.DOI: 10.1093/schbul/sbv133.
[7]	KAGEYAMA Y, KASAHARA T, NAKAMURA T, et al. Plasma nervonic acid is a potential biomarker for major depressive disorder:a pilot study[J]. Int J Neuropsychopharmacol, 2018,21(3):207-215.DOI: 10.1093/ijnp/pyx089.
[8]	于震宇. 元宝枫愈伤组织培养及其黄酮类化合物积累的研究[D]. 杨凌:西北农林科技大学, 2004.
	YU Z Y. Studies on callus culture and flavonoid formation of Acer Truncatum Bunge[D]. Yangling:Northwest & University, 2004.
[9]	李艳菊, 陶加洪, 王兰珍, 等. 元宝枫组织培养研究[J]. 北京林业大学学报, 2005,27(3):104-107.
	LI Y J, TAO J H, WANG L Z, et al. Tissue culture of Acer truncatum[J]. J Beijing For Univ, 2005,27(3):104-107.DOI: 10.3321/j.issn:1000-1522.2005.03.022.
[10]	袁云香, 商澎. 小果卫矛组织培养与快速繁殖[J]. 植物生理学报, 2020,56(6):1243-1247.
	YUAN Y X, SHANG P. Tissue culture and rapid propagation of Euonymus microcarpus[J]. Plant Physiology Journal, 2020,56(6):1243-1247. DOI: 10.13592/j.cnki.ppj.2020.0100.
[11]	刘占彬, 袁庆华. 多花黑麦草种子外植体组织培养灭菌方法研究[J]. 草地学报, 2009,17(4):474-479.
	LIU Z B, YUAN Q H. Study on the sterilization methods in tissue culture of Italian ryegrass seed explant[J]. Acta Agrectir Sin, 2009,17(4):474-479.DOI: 10.11733/j.issn.1007-0435.2009.04.012.
[12]	张帆, 叶露, 董姬妃, 等. 高盆樱桃的组培快繁研究[J]. 南京林业大学学报(自然科学版), 2018,42(3):111-116.
	ZHANG F, YE L, DONG J F, et al. Study on tissue culture and rapid propagation of Cerasus cerasoides var.cerasoides[J]. J Nanjing For Univ (Nat Sci Ed), 2018,42(3):111-116.DOI: 10.3969/j.issn.1000-2006.201706047.
[13]	官锦燕, 谭嘉娜, 罗剑飘, 等. 牛樟的组织培养和植株再生[J]. 南京林业大学学报(自然科学版), 2016,40(4):63-68.
	GUAN J Y, TAN J N, LUO J P, et al. Tissue culture and plant regeneration of Cinnamomum kanehirae Hay[J]. J Nanjing For Univ (Nat Sci Ed), 2016,40(4):63-68.DOI: 10.3969/j.issn.1000-2006.2016.04.010.
[14]	张静, 牛喆, 范卫芳, 等. 狭叶黄芩组织培养再生体系的建立[J]. 植物研究, 2020,40(1):50-57.
	ZHANG J, NIU Z, FAN W F, et al. Establishing tissue culture and regeneration system of Scutellaria regeliana[J]. Bull Bot Res, 2020,40(1):50-57.
[15]	苏梦云. 美国枫香茎段组织培养与植株再生[J]. 林业科学研究, 2005,18(1):98-101.
	SU M Y. Tissue culture of stem segments and plantlet regenation of Liquidambar styraciflua[J]. For Res, 2005,18(1):98-101.
[16]	史港影, 南程慧, 伊贤贵, 等. 雪落樱再生体系的建立[J]. 南京林业大学学报(自然科学版), 2014(S1):20-24.
	SHI G Y, NAN C H, YI X G, et al. Establishment of regeneration system in Cerasus xueluoensis[J]. J Nanjing For Univ (Nat Sci Ed), 2014(S1):20-24. DOI: 10.3969/j.issn.1000-2006.2014.S1.005.
[17]	牟海飞, 刘洁云, 黄永才, 等. 火龙果离体培养茎段灭菌及芽诱导增殖研究[J]. 西南农业学报, 2017,30(6):1439-1444.
	MOU H F, LIU J Y, HUANG Y C, et al. Study on stem disinfection,bud induction and proliferation of Hylocereus undatus in vitro culture[J]. Southwest China J Agric Sci, 2017,30(6):1439-1444.DOI: 10.16213/j.cnki.scjas.2017.6.034.
[18]	李艳敏, 孟月娥, 赵秀山, 等. 金叶复叶槭组织培养技术研究[J]. 河南农业科学, 2008,37(7):98-99.
	LI Y M, MENG Y E, ZHAO X S, et al. Studies on techniques of tissue culture and rapid propagation of Acer negundo ‘Aureomarginatum’[J]. J Henan Agric Sci, 2008,37(7):98-99.DOI: 10.3969/j.issn.1004-3268.2008.07.031.
[19]	李岩岩. 茶条槭组织培养与快速繁殖技术研究[J]. 山东林业科技, 2010,40(2):48-50.
	LI Y Y. Studies on techniques of tissue culture and rapid propagation of Acer ginnala[J]. J Shandong For Sci Technol, 2010,40(2):48-50.DOI: 10.3969/j.issn.1002-2724.2010.02.015.
[20]	顾地周, 丛小力, 姜云天, 等. 色木槭的组织培养与快速繁殖[J]. 植物生理学通讯, 2008,44(2):314.
	GU D Z, CONG X L, JIANG Y T, et al. Tissue culture and rapid propagation of Acer mono Maxim[J]. Plant Physiol Commun, 2008,44(2):314.
[21]	王盼盼. 黑木相思不同优良无性系组培快繁技术比较研究[D]. 福州: 福建农林大学, 2011.
	WANG P P. A comparative study on the techniques of tissue culture and rapid propagation in different fine clones of Acacia melanoxylon[D]. Fuzhou:Fujian Agriculture and Forestry University, 2011.
[22]	李玲莉, 郭素娟, 李吉跃. 北美柔枝松组织培养研究[J]. 北京林业大学学报, 2010,32(3):209-212.
	LI L L, GUO S J, LI J Y. Tissue culture protocol of limber pine of north America[J]. J Beijing For Univ, 2010,32(3):209-212.
[23]	陈茜, 范佳露, 王宝腾, 等. 落叶型冬青雌、雄株茎段的组织培养技术[J]. 南京林业大学学报(自然科学版), 2017,41(6):181-186.
	CHEN X, FAN J L, WANG B T, et al. Micropropagation of Ilex verticillata axillary buds from male and female seedlings[J]. J Nanjing For Univ (Nat Sci Ed), 2017,41(6):181-186.DOI: 10.3969/j.issn.1000-2006.201704002.
[24]	宋跃朋, 陈盼飞, 卜琛皞, 等. 高产优质毛白杨良种组培繁育体系构建[J]. 北京林业大学学报, 2019,41(7):121-127.
	SONG Y P, CHEN P F, PU C G, et al. Construction of tissue culture breeding system of high yield and quality species in Populus tomentosa[J]. J Beijing For Univ, 2019,41(7):121-127. DOI: 10.13332/j.1000-1522.20180428.

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处理 treatment	处理时间/s treatment time		污染率/% pollution ratio	死亡率/% death ratio	成活率/% survival ratio
处理 treatment	75% C₂H₅OH	0.1% HgCl₂	污染率/% pollution ratio	死亡率/% death ratio	成活率/% survival ratio
1	10	120	93.3±0.0 aA	2.6±0.5 dCD	4.1±0.5 ff
2	10	180	80.7±1.7 bB	3.3±0.0 bcdBC	15.9±1.7 eE
3	10	240	56.7±0.0 cC	2.6±0.5 dCD	40.7±0.5 dD
4	20	120	60.0±2.2 cC	3.0±0.5 cdBC	37.0±2.5 dD
5	20	180	45.6±1.5 dD	3.3±0.0 bcdBC	51.1±1.5 cC
6	20	240	37.8±1.5 eE	3.7±0.5 bcdBC	58.5±1.2 bB
7	30	120	44.4±3.0 dD	4.1±0.5 bcBC	51.5±2.7 cC
8	30	180	11.7±0.5 ff	4.4±0.0 bB	83.9±0.5 aA
9	30	240	9.3±0.5 ff	28.9±0.7 aA	61.9±1.2 bB

处理 treatment	采集时期 collected periods	采集地 collected plot	污染率/% pollution ratio	死亡率/% death ratio	成活率/% survival ratio
1	5月	室外大田	8.9±1.5 eD	1.5±0.5 bA	89.6±0.7 aA
2	5月	温室盆栽	7.8±1.5 eD	1.9±0.5 abA	90.4±0.8 aA
3	7月	室外大田	25.6±1.5 cB	3.3±0.7 aA	71.1±0.7 cC
4	7月	温室盆栽	18.9±0.3 dC	3.0±0.5 abA	78.1±0.9 bB
5	9月	室外大田	94.4±1.5 aA	2.2±0.7 abA	3.3±0.5 dD
6	9月	温室盆栽	31.1±1.5 bB	3.0±0.5 abA	65.9±3.5 eE

处理 treatment	培养基 culture mediums	IBA质量浓度/ (mg·L^-1) IBA mass content	出芽率/% induction ratio	腋芽数量 (>0.5 cm) cluster bud number	腋芽展叶时间/d axillary bud spreading time
1	1/2 MS	0	77.8±3.7 deG	1.5	30~35
2	1/2 MS	0.2	86.7±1.1 cBCDE	1.6	25~30
3	1/2 MS	0.4	85.4±0.7 cDEF	1.7	25~30
4	MS	0	75.6±0.7 eG	1.4	30~35
5	MS	0.2	92.2±0.7 bAB	1.8	25~30
6	MS	0.4	87.8±0.7 cBCD	1.7	25~30
7	NN69	0	88.3±0.0 bcABC	1.7	25~30
8	NN69	0.2	97.2±1.9 aA	1.9	10~15
9	NN69	0.4	93.9±0.7 abAB	1.9	10~15
10	1/2 NN69	0	81.1±4.1 cdFG	1.6	25~30
11	1/2 NN69	0.2	92.2±0.7 bAB	1.8	20~25
12	1/2 NN69	0.4	92.8±0.7 bAB	1.8	20~25

处理 treatment	培养基 culture medium	IBA/ (mg·L^-1)	生根率/% rooting ratio	根数/条 root number	根系生长状况 status of roots
1	1/2MS	0.2	47.8±1.5 deC	2.1±0.1 fE	长势稍差
2	1/2MS	0.4	66.7±1.5 deC	3.5±0.1 cC	长势一般
3	1/2MS	0.6	50.4±0.5 dC	2.1±0.1 ff	长势稍差
4	MS	0.2	41.9±2.7 efDE	2.1±0.2 ff	长势稍差
5	MS	0.4	50.0±0.7 dC	3.2±0.2 eCD	长势一般
6	MS	0.6	44.8±0.5 eCD	3.5±0.0 cdC	长势一般
7	NN69	0.2	33.3±4.4 gF	1.7±0.0 gF	长势稍差
8	NN69	0.4	47.4±1.0 deC	2.3±0.0 fE	长势稍差
9	NN69	0.6	36.7±0.7 gEF	1.6±0.1 gf	长势稍差
10	1/2NN69	0.2	70.7±1.2 bB	4.3±0.2 bB	长势最好
11	1/2NN69	0.4	87.8±0.7 aA	5.2±0.1 aA	长势最好
12	1/2NN69	0.6	65.9±0.5 cB	3.8±0.2 cC	长势稍差

Study on tissue culture and rapid propagation of Acer truncatum Bunge

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