Study on tissue culture and rapid propagation of Acer truncatum Bunge

MA Qiuyue, LI Qianzhong, LI Shushun, ZHU Lu, YAN Kunyuan, LI Shuxian, ZHANG Bin, WEN Jing

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (2) : 220-224.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (2) : 220-224. DOI: 10.12302/j.issn.1000-2006.202009049

Study on tissue culture and rapid propagation of Acer truncatum Bunge

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Abstract

【Objective】To establish a rapid propagation system, disinfection method and optimal medium were screened, which will lay the foundation for research on the breeding of Acer truncatum seeding. 【Method】The effects of different sterilization treatment mehods, basic media (1/2 MS, MS, 1/2 NN69, NN69), and plant hormones (IBA) on axillary buds and rooting were investigated using stems with axillary buds of A. truncatum. 【Result】When the stem with axillary buds was sterilized with 75% (volume fraction) alcohol for 30 s and 0.1% (mass fraction) HgCl2 for 180 s, the survival rate was the highest. The NN69 medium supplemented with 0.2 mg/L IBA showed the highest axillary bud induction rate (97.2%) with the shortest time (10-15 d). The 1/2 NN69 medium supplemented with 0.4 mg/L IBA was suitable for plantlet rooting, with a rooting rate of 87.8%. 【Conclusion】The optimal sterilization method for stem segments of A. truncatum with axillary buds was 75% alcohol for 30 s and 0.1% HgCl2 for 180 s. The optimal medium for axillary bud induction was NN69 with 0.2 mg/L IBA, and the optimal rooting medium was 1/2 NN69 with 0.4 mg/L IBA. The successful cultivation substrate was prepared by mixing peat, humus,and roseite at a volume ratio of 7:2:1, and the survival rate was 95.0% when the plantlets were moved to the nursery. The results of this study can provide a basis for large-scale propagation of A. truncatum.

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Acer truncatum / tissue culture / rooting / rapid propagation

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MA Qiuyue , LI Qianzhong , LI Shushun , et al . Study on tissue culture and rapid propagation of Acer truncatum Bunge[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2021, 45(2): 220-224 https://doi.org/10.12302/j.issn.1000-2006.202009049

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