Application of CRISPR/Cas technique in woody plant improvement

HOU Jing, MAO Jinyan, ZHAI Hui, WANG Jie, YIN Tongming

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (6) : 24-30.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (6) : 24-30. DOI: 10.12302/j.issn.1000-2006.202010017

Application of CRISPR/Cas technique in woody plant improvement

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Abstract

Long breeding periods and scarce germplasm resources have become the main bottlenecks in woody plant improvement. Recently, the CRISPR/Cas system has been developed into a precision site-directed editing technology that has been widely used in the breeding of woody plants such as Populus spp., Malus spp., Citrus spp., Vitis vinifera and Cassava spp.. The yield, quality, biotic- and abiotic-stress resistance, and other traits of woody plants have been significantly improved by genome editing, achieving the fixation of excellent genotypes within a single generation. This approach has accelerated the breeding process of woody plants and improves their breeding efficiency. Here, we summarize the application of CRISPR/Cas technology to woody plants, analyzing existing problems and future development trends. This review aims to provide a useful reference for gene function research and improvement of woody plants.

Key words

CRISPR/Cas / genome editing / molecular breeding / woody plants / breed improvement

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HOU Jing , MAO Jinyan , ZHAI Hui , et al . Application of CRISPR/Cas technique in woody plant improvement[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2021, 45(6): 24-30 https://doi.org/10.12302/j.issn.1000-2006.202010017

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