Responses of the non-structural carbohydrates and rhizosphere soil enzymes of Clematis fruticosa to nitrogen deposition and inoculation mycorrhizal fungi

doi:10.12302/j.issn.1000-2006.202103006

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (1): 171-178.doi: 10.12302/j.issn.1000-2006.202103006

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Responses of the non-structural carbohydrates and rhizosphere soil enzymes of Clematis fruticosa to nitrogen deposition and inoculation mycorrhizal fungi

ZHANG Xiaorong¹(), DUAN Guangde¹, HAO Longfei¹^,^*(), LIU Tingyan¹, ZHANG You², ZHANG Shengxi¹

1. College of Forestry, Inner Mongolia Agricultural University, Hohhot 010019, China
2. Inner Mongolia Forestry Workstation, Hohhot 010020, China

Received:2021-03-02 Accepted:2021-09-27 Online:2022-01-30 Published:2022-02-09
Contact: HAO Longfei E-mail:ZHANGXR9703@163.com;haolongfei_00@126.com

Abstract

Abstract:

【Objective】The responses of plant rhizosphere soil enzymes and non-structural carbohydrates (NSC) were investigated in relation to nitrogen deposition and inoculation with mycorrhizal fungi. Strategies for the rhizosphere micro-ecological environment and plant growth were explored in the context of changes to global nitrogen deposition.【Method】 One year old Clematis fruticosa mycorrhizal and non-mycorrhizal seedlings (serial No.-M) were subjected to microcosm experiments. The inoculation treatments included single inoculation (Rhizophagus intraradices, serial No. +R; Funneliformis mosseae, serial No. +F) and mixed inoculation (R. intraradices and F. mosseae 1:1 mixture fungi agents, serial No. +RF). Four nitrogen deposition treatments were established: no nitrogen [CK, 0 g/(m²·a)], low nitrogen [LN, 3 g/(m²·a)], medium nitrogen [MN, 6 g/(m²·a) ] and high nitrogen [HN, 9 g/(m²·a)]. The NSC [i.e., soluble sugar (SS) and starch (ST)], and rhizosphere soil enzymes [i.e., β-1, 4-glucosidase (BG), leucine aminopeptidase (LAP), β-1,4-N-acetylglucosaminidase (NAG), acid phosphatase (ACP), and alkaline phosphatase (ALP)] of seedlings were determined following the inoculation and nitrogen deposition treatments. One-way analysis of variance, and two-factor interaction and correlation analyses were used to determine the effect of different inoculation treatments with increasing nitrogen deposition in terms of the carbon, nitrogen and phosphorus related enzymatic activities in the seedlings rhizosphere. Additionally, the allocation of NSCs in seedlings organs were explored in response to the inoculation with mycorrhizal fungi and nitrogen deposition.【Result】① In addition to BG activity in the soil rhizosphere of C. fruticosa seedlings, the nitrogen deposition, inoculation with mycorrhizal fungi, and their interactions notably affected the nitrogen and phosphorus related enzymes. Under HN treatment, the NAG activity in the soil rhizosphere had notably decreased through the inoculation treatments. The ACP and ALP activities related to the soil rhizosphere phosphorus had notably increased under the -M and HN treatments. The ALP activity peaked in the HN treatment under the +R and +F treatments. ② The NSC content of C. fruticosa seedlings was notably affected by nitrogen deposition, inoculation with mycorrhizal fungi, and their interactions. Under the nitrogen deposition treatments, the contents of SS, ST and NSC of seedlings in the inoculation treatments were higher than those without the inoculation treatment; the SS, ST and NSC contents peaked in the +F treatment. ③ The seedling NSC content in the -M treatment in ascending order was: stem < root < leaf, while that for the inoculation treatments (i.e., LN, MN, and HN) was: root < stem < leaf. Under the HN treatment, the ST and NSC contents in roots, and the SS and NSC contents in the stems and leaves of seedlings peaked in the +F treatment. ④ The SS, ST and NSC contents were notably negatively correlated with soil nitrogen-related NAG activity; however, they were notably positively correlated with soil phosphorus and carbon-related enzymes under nitrogen deposition and inoculation with mycorrhizal fungi. The correlation coefficient was the maximum between the SS and ST contents and the ALP activities related to phosphorus, while it was at a minimum between the SS and ST contents and the BG activities related to carbon under the nitrogen deposition and inoculation treatments.【Conclusion】The effect of nitrogen and phosphorus-related enzymatic activities in the soil rhizosphere of C. fruticosa seedlings under nitrogen deposition and inoculation treatments was higher than carbon-related enzymatic activities. Nitrogen deposition notably enhanced the phosphatase activity in the soil rhizosphere of mycorrhizal seedlings. Inoculation treatments increased the NSC content of seedlings under nitrogen deposition, the most significant effect being observed for the mycorrhizal fungi of F. mosseae. The distribution of NSC in seedling roots had increased notably under a high nitrogen environment.

Key words: Clematis fruticosa, arbuscular mycorrhizal fungi, nitrogen deposition, soil enzyme activity, non-structural carbohydrates

CLC Number:

S718
S154.36

ZHANG Xiaorong, DUAN Guangde, HAO Longfei, LIU Tingyan, ZHANG You, ZHANG Shengxi. Responses of the non-structural carbohydrates and rhizosphere soil enzymes of Clematis fruticosa to nitrogen deposition and inoculation mycorrhizal fungi[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(1): 171-178.

Figures/Tables 3

Table 1

Fig.1

Table 2

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接菌处理 inoculation mycorrhizal fungi	模拟氮沉降 nitrogen deposition	β-1,4 葡萄糖苷酶活性 β-1, 4- glucosidase (BG) activity	亮氨酸氨基肽酶活性 leucine aminopeptidase (LAP) activity	β-1,4-N-乙酰- 氨基葡糖苷酶活性 β-1,4-N-acetylglu- cosaminidase (NAG) activity	酸性磷酸酶活性 acid phosphatase (ACP) activity	碱性磷酸酶活性 alkaline phosphatase (ALP) activity
-M	CK	1.70±0.56 Bc	0.08±0.08 Cb	61.53±4.29 Aa	12.61±1.23 Bc	21.91±2.16 Bb
	LN	2.16±0.20 Bb	3.23±0.69 Bb	54.50±6.63 Aa	13.47±1.53 Bb	22.11±0.75 Bc
	MN	4.26±0.73 ABa	7.69±0.23 Aa	34.56±5.35 Ba	12.49±0.62 Bb	21.35±1.70 Bb
	HN	6.10±1.48 Aa	6.09±1.07 Aa	25.77±4.45 Ba	24.14±0.95 Aa	30.47±1.43 Ac
+R	CK	6.68±0.85 Aab	5.56±0.93 ABa	34.40±7.15 Bb	12.12±1.22 Bc	32.81±0.92 Aa
	LN	5.37±1.85 Aab	7.74±0.48 Aa	60.07±2.75 Aa	25.50±3.67 Aa	32.21±0.59 Ab
	MN	4.83±0.79 Aa	3.01±0.59 Bc	23.57±4.92 Ba	18.00±0.22 ABb	31.72±0.42 Aa
	HN	3.72±0.66 Aa	5.23±1.69 ABa	2.80±1.00 Cb	17.96±2.40 ABb	33.43±0.73 Abc
+F	CK	4.54±0.69 Abc	5.70±1.09 Ba	3.44±1.21 Bc	28.66±0.49 ABa	35.23±0.73 Ba
	LN	7.02±0.47 Aab	8.25±0.29 Aa	8.60±1.37 Ab	27.60±1.52 Ba	32.00±0.20 Cb
	MN	5.51±1.93 Aa	7.22±0.15 ABa	6.78±0.90 ABb	32.03±1.24 Aa	34.63±1.46 BCa
	HN	4.18±0.76 Aa	8.48±0.60 Aa	8.14±0.51 Ab	26.22±1.03 Ba	39.40±0.74 Aa
+RF	CK	8.75±1.75 Aa	5.20±0.36 Aa	1.92±0.19 Cc	22.81±1.81 ABb	32.48±0.72 Ba
	LN	8.75±1.75 Aa	4.00±0.25 Bb	2.91±0.50 BCb	24.24±0.78 Aa	37.18±0.54 Aa
	MN	8.18±1.52 Aa	5.42±0.10 Ab	4.18±0.68 Bb	26.49±3.26 Aa	33.65±1.44 Ba
	HN	7.52±1.82 Aa	5.76±0.29 Aa	6.35±0.26 Ab	17.16±1.43 Bb	35.90±1.10 ABb

指标 index	β-1,4 葡萄糖苷酶 β-1, 4- glucosidase (BG)	亮氨酸氨基肽酶 leucine aminopeptidase (LAP)	β-1,4-N-乙酰- 氨基葡糖苷酶 β-1,4-N- acetylglucosaminidase (NAG)	酸性磷酸酶 acid phosphatase (ACP)	碱性磷酸酶 alkaline phosphatase (ALP)
可溶性糖含量 soluble sugar (SS)	0.368^*	0.436^**	-0.659^**	0.655^**	0.834^**
淀粉含量 starch (ST)	0.286^*	0.443^**	-0.595^**	0.642^**	0.825^**
非结构性碳水化合物含量 non-structural carbohydrates (NSC)	0.323^*	0.430^**	-0.649^**	0.650^**	0.835^**

Responses of the non-structural carbohydrates and rhizosphere soil enzymes of Clematis fruticosa to nitrogen deposition and inoculation mycorrhizal fungi

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