Effects of the four genes expressions and photosynthetic parameters on seedlings growth of different birch clones

doi:10.12302/j.issn.1000-2006.202103037

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (2): 88-94.doi: 10.12302/j.issn.1000-2006.202103037

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Effects of the four genes expressions and photosynthetic parameters on seedlings growth of different birch clones

NA Xiaoying(), LIU Gang, LIU Guifeng, WANG Xiuwei()

Key Laboratory of Sustainable Forest Ecosystem Management, Ministry of Education, College of Forestry, Northeast Forestry University, Harbin 150040, China

Received:2021-03-22 Accepted:2021-08-20 Online:2022-03-30 Published:2022-04-08
Contact: WANG Xiuwei E-mail:nxy_myself@163.com;wxgreat@nefu.edu.cn

Abstract

Abstract:

【Objective】 We analyzed the relationship among RBCS1A, RBCS3B, GO and RCA gene expression in one-year-old white birch clone seedlings and photosynthetic rate, transpiration rate, stomatal conductance, instantaneous water use efficiency, intercellular CO₂ concentration, carboxylation efficiency, seedling height, and ground diameter of seedlings. Moreover, the effects of gene expression and photosynthetic parameters on the initial growth of birch clone seedlings were preliminarily explored. 【Method】 Six mature trees in a diallel cross progeny forest of white birch were cultured and propagated as six clones, and growth, photosynthetic parameters and expression of four photosynthesis-related genes in one-year-old white birch clone seedlings were determined and analyzed. 【Result】 There were significant differences in growth, photosynthetic parameters, and relative gene expression in seedlings of six different genotypes of white birch clones (P < 0.05). A significant correlation between photosynthetic parameters was observed, and the net photosynthetic rate was significantly positively correlated with instantaneous water use efficiency, transpiration rates, stomatal conductance, and carboxylation efficiency (P < 0.05); the relative expression of RBCS1A was significantly positively correlated with RBCS3B. The relative expression of RBCS1A was significantly positively correlated with RCA, and the relative expression of the GO gene was significantly negatively correlated with RCA (P < 0.05). A significant correlation between the relative gene expression and the plants’ transpiration rate, net photosynthetic rate, instantaneous water use efficiency, and stomatal conductance was observed (P < 0.05); however, the difference between the relative gene expression and photosynthetic parameters was not significantly correlated with the plant height and ground diameter (P < 0.05). 【Conclusion】 The expression levels of four genes and six photosynthetic parameters of different one-year-old white birch clone seedlings were significantly different and showed strong correlations, however, the correlation between the seedling height and ground diameter was not significant. This showed that in the early stage of birch seedling growth, the expression of the four examined genes and six photosynthetic parameters did not significantly affect the growth.

Key words: Betula platyphylla Suk. clone, seedling, growth, gene expression, photosynthetic parameters

CLC Number:

S718
S722.3

NA Xiaoying, LIU Gang, LIU Guifeng, WANG Xiuwei. Effects of the four genes expressions and photosynthetic parameters on seedlings growth of different birch clones[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(2): 88-94.

Figures/Tables 6

Table 1

Table 2

Fig.1

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引物名 primer name	引物序列 primer sequence	温度/℃ temperature	产物长度/bp product length
BpGO-F	GGAGATTACCAATGTCAGCGAGTAT	68.1	77
BpGO-R	ACGCATAGTAGTCATACACCATCTTG	68.1	77
BpRCA-F	TGTCCAAGAGCAAGAGAATGTGAA	68.2	91
BpRCA-R	AATAGCATCCTGGTTAGCATCTCC	68.1	91
BpRBCS1A-F	CCAGAAGACCAACAACGACATTAC	67.6	126
BpRBCS1A-R	GAACTAAGTGGTGGAAGGTAGGAA	67.5	126
BpRBCS3B-F	GCAAGCTCAACAATGACATTACCT	67.7	97
BpRBCS3B-R	GTCTCAAACTTCTTCAATCCAACTGG	67.9	97
BpUBC-F	GGTTTCTGGGTTACAGTAAAGTTTCAG	67.7	76
BpUBC-R	CTTGAAGCCTGCATGTGGTC	67.8	76
BpSAND-F	CACAGAAGAGCCTTACGAGTCAT	67.9	135
BpSAND-R	CCTCCAAGCAAAGATGTCATATCAAAC	68.1	135

Effects of the four genes expressions and photosynthetic parameters on seedlings growth of different birch clones

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温度/℃ temperature	时间/s time	循环数 number of cycles
95	60	1
95	15	45
60	60	45
95	60	1
55	30	1
95	30	1