PDF(16093 KB)
Diversity of microbial community in rhizosphere of genetically modified Populus simonii × P. nigra
WANG Yang, WANG Wei, JIANG Jing, GU Chenrui, YANG Yunli
JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2023, Vol. 47 ›› Issue (1) : 199-208.
PDF(16093 KB)
PDF(16093 KB)
Diversity of microbial community in rhizosphere of genetically modified Populus simonii × P. nigra
【Objective】 The composition of bacterial and fungal communities in rhizosphere soil is not only affected by plant species and varying growth periods, but also by the introduction of foreign genes. The contents of betaine or late-embryogenesis-abundant proteins could be increased in transgenic poplar with betA and TaLEA genes, which were obtained by genetic engineering technology. Drought resistance and salt tolerance were also enhanced in these transgenic poplars. PsnWRKY70 is a negative regulator of the salt stress response signal transduction network. Salt tolerance of WRKY70 inhibitory expression line was significantly higher than that of the line wild type (WT). To clarify the drought resistance and salt tolerance of transgenic poplar with betA, TaLEA and WRKY70, the composition of rhizosphere soil bacterial and fungal communities of transgenic poplar was analyzed to provide a reference for its environmental and ecological security evaluation. 【Method】 In this study, rhizosphere soil was collected from a two-year-old poplar with modified TaLEA, betA and WRKY70 genes, and WT plants were used as controls. The 16S rRNA and ITS sequences of microorganisms in the rhizosphere soil were sequenced using the Illumina-MiSeq highthroughput sequencing platform. The population structures and community compositions of the bacteria and fungi in the rhizosphere soil were then determined and analyzed. The purpose of this study was to understand how transgenic poplar influences the composition of soil microbial communities before the subsequent environmental release. 【Result】 For the composition of bacterial communities, Alpha diversity showed that the Simpson index of betA transgenic poplar was significantly lower than that of the WT, the Shannon and Simpson indexes of WRKY70 transgenic poplar were also significantly lower than that of WT. The fungal indices of the transgenic poplars were significantly lower than those of the WT. The Venn diagram, principal component analysis, and cluster heat map showed that there was little difference in the microbial community structure between TaLEA and WT plants. The microbial communities of transgenic betA and WRKY70 poplars were similar. Based on the composition and abundance analysis at the genus level in the rhizosphere soil collected from transgenic poplars, the abundance of beneficial bacteria, such as Rhodanobacter and Gemmatimonas, increased to varying degrees. The abundance of the beneficial mycorrhizal fungi Inocybe in TaLEA and betA plants was significantly higher than that in the other two samples. The abundance of Sphaerosporella, Hebeloma and Laccaria significantly increased only in TaLEA plants. Conversely, the abundance of pathogenic fungi, such as Didymella and Cadophora, decreased by more than 85% compared with the WT. 【Conclusion】 The diversity of rhizosphere microorganisms was influenced by transgenic poplars, which may be beneficial to plant growth and enhance stress resistance. However, more research is needed to prove whether these microorganisms play a vital role.
transgenic Populus simonii × P. nigra / rhizosphere / bacteria / fungi / community diversity
| [1] |
田颖川, 李太元, 莽克强, 等. 抗虫转基因欧洲黑杨的培育[J]. 生物工程学报, 1993, 9(4):291-297,395.
|
| [2] |
卢孟柱, 胡建军. 我国转基因杨树的研究及应用现状[J]. 林业科技开发, 2006, 20(6):1-4.
|
| [3] |
|
| [4] |
吕威, 卢楠, 侯荣轩, 等. 12年生转基因毛白杨外源基因转移及其对土壤微生物的影响[J]. 东北林业大学学报, 2018, 46(9):1-6.
|
| [5] |
孙伟博, 魏朝琼, 马晓星, 等. 3类转基因‘南林895’杨田间试验的安全性评估[J]. 林业科学, 2020, 56(10):53-62.
|
| [6] |
胡建军, 张蕴哲, 卢孟柱, 等. 欧洲黑杨转基因稳定性及对土壤微生物的影响[J]. 林业科学, 2004, 40(5):105-109.
|
| [7] |
马晓星, 孙伟博, 魏辉, 等. 转AtSnRK2C基因杨树田间试验及生物安全性分析[J]. 分子植物育种, 2019, 17(11):3570-3578.
|
| [8] |
吕威, 孙宇涵, 张华新, 等. 转基因三倍体毛白杨花粉活力及枯落物外源基因检测[J]. 北京林业大学学报, 2019, 41(7):91-100.
|
| [9] |
汤沂, 向东, 裘芳, 等. 转基因植物的环境及食品安全性研究[J]. 现代食品, 2019(15):126-127,130.
|
| [10] |
陈静怡, 刘瑞华, 王丽丽, 等. 磷高效转基因水稻连续种植对土壤微生物功能多样性的影响[J]. 天津农学院学报, 2019, 26(2):1-7.
|
| [11] |
|
| [12] |
|
| [13] |
|
| [14] |
|
| [15] |
朱文旭, 丁昌俊, 张伟溪, 等. 8年生转基因库安托杨外源基因转移及对土壤微生物数量影响的检测[J]. 林业科学研究, 2017, 30(2):349-353.
|
| [16] |
周培军, 李玲玲, 李红岩, 等. 转Bt基因‘南林895’杨时空表达及生物安全分析[J]. 分子植物育种, 2022, 20(5):1568-1580.
|
| [17] |
侯英杰, 苏晓华, 焦如珍, 等. 转基因银腺杂种杨对土壤微生物的影响[J]. 林业科学, 2009, 45(5):148-152.
|
| [18] |
吕秀华. 转基因银中杨ABJ系列对土壤微生物类群的影响[J]. 基因组学与应用生物学, 2017, 36(5):1991-1996.
|
| [19] |
吕秀华. 转基因银中杨对根际土壤微生物的影响[J]. 基因组学与应用生物学, 2018, 37(5):1965-1970.
|
| [20] |
马晓星, 孙伟博, 魏辉, 等. 转PeTLP基因‘南林895’杨对土壤微生物的影响及外源基因分子检测[J]. 浙江林业科技, 2018, 38(4):28-37.
|
| [21] |
杨传平, 刘桂丰, 梁宏伟, 张慧. 耐盐基因Bet-A转化小黑杨的研究[J]. 林业科学, 2001, 37(6):34-38.
|
| [22] |
|
| [23] |
杜金友, 陈晓阳, 李伟, 等. 林木抗旱的渗透调节及其基因工程研究进展[J]. 西北植物学报, 2004, 24(6):1154-1159.
|
| [24] |
刘桂丰, 杨传平, 蔡智军, 等. 转bet A基因小黑杨的耐盐性分析及优良转基因株系的选择[J]. 林业科学, 2006, 42(7):33-36.
|
| [25] |
孙延爽, 邢宝月, 杨光, 等. NaHCO3胁迫对转TaLEA基因山新杨生长及光合、叶绿素荧光特性的影响[J]. 北京林业大学学报, 2017, 39(10):33-41.
|
| [26] |
|
| [27] |
李志新, 赵曦阳, 杨成君, 等. 转TaLEA基因小黑杨株系变异及生长稳定性分析[J]. 北京林业大学学报, 2013, 35(2):57-62.
|
| [28] |
葛梦妍, 顾宸瑞, 陈坤, 等. 转betA基因小黑杨生长及适应性[J]. 东北林业大学学报, 2021, 49(4):12-16,23.
|
| [29] |
|
| [30] |
There is considerable interest in understanding the biological mechanisms that regulate carbon exchanges between the land and atmosphere, and how these exchanges respond to climate change. An understanding of soil microbial ecology is central to our ability to assess terrestrial carbon cycle-climate feedbacks, but the complexity of the soil microbial community and the many ways that it can be affected by climate and other global changes hampers our ability to draw firm conclusions on this topic. In this paper, we argue that to understand the potential negative and positive contributions of soil microbes to land-atmosphere carbon exchange and global warming requires explicit consideration of both direct and indirect impacts of climate change on microorganisms. Moreover, we argue that this requires consideration of complex interactions and feedbacks that occur between microbes, plants and their physical environment in the context of climate change, and the influence of other global changes which have the capacity to amplify climate-driven effects on soil microbes. Overall, we emphasize the urgent need for greater understanding of how soil microbial ecology contributes to land-atmosphere carbon exchange in the context of climate change, and identify some challenges for the future. In particular, we highlight the need for a multifactor experimental approach to understand how soil microbes and their activities respond to climate change and consequences for carbon cycle feedbacks.
|
| [31] |
徐征. 农业转基因生物对土壤生态系统功能影响的研究进展[J]. 中国农学通报, 2004, 20(4):47-50,72.
|
| [32] |
|
| [33] |
|
| [34] |
|
| [35] |
陈晓雯, 林胜, 尤民生, 等. 转基因水稻对土壤微生物群落结构及功能的影响[J]. 生物安全学报, 2011, 20(2):151-159.
|
| [36] |
Microbial community dynamics were characterized following combined oxidation and biodegradation treatment trains for mixtures of 1,4-dioxane and chlorinated volatile organic compounds (CVOCs) in laboratory microcosms. Bioremediation is generally inhibited by co-contaminate CVOCs; with only a few specific bacterial taxa reported to metabolize or cometabolize 1,4-dioxane being unaffected. Chemical oxidation by hydrogen peroxide (HO) as a non-selective treatment demonstrated 50-80% 1,4-dioxane removal regardless of the initial CVOC concentrations. Post-oxidation bioaugmentation with 1,4-dioxane metabolizer Pseudonocardia dioxanivorans CB1190 removed the remaining 1,4-dioxane. The intrinsic microbial population, biodiversity, richness, and biomarker gene abundances decreased immediately after the brief oxidation phase, but recovery of cultivable microbiomes and a more diverse community were observed during the subsequent 9-week biodegradation phase. Results generated from the Illumina Miseq sequencing and bioinformatics analyses established that generally oxidative stress tolerant genus Ralstonia was abundant after the oxidation step, and Cupriavidus, Pseudolabrys, Afipia, and Sphingomonas were identified as dominant genera after aerobic incubation. Multidimensional analysis elucidated the separation of microbial populations as a function of time under all conditions, suggesting that temporal succession is a determining factor that is independent of 1,4-dioxane and CVOCs mixtures. Network analysis highlighted the potential interspecies competition or commensalism, and dynamics of microbiomes during the biodegradation phase, in line with the shifts of predominant genera and various developing directions during different steps of the treatment train. Collectively, this study demonstrated that chemical oxidation followed by bioaugmentation is effective for treating 1,4-dioxane, even in the presence of high levels of CVOC mixtures and residual peroxide, a disinfectant, and enhanced our understanding of microbial ecological impacts of the treatment train. These results will be valuable for predicting treatment synergies that lead to cost savings and improved remedial outcomes in short-term active remediation as well as long-term changes to the environmental microbial communities.Copyright © 2018 Elsevier Ltd. All rights reserved.
|
| [37] |
赖宝春, 戴瑞卿, 吴振强, 等. 辣椒健康植株与患枯萎病植株根际土壤细菌群落多样性的比较研究[J]. 福建农业学报, 2019, 34(9):1073-1080.
|
| [38] |
|
| [39] |
|
| [40] |
|
| [41] |
|
| [42] |
Microbial community DNA was extracted from activated sludge samples taken from a chemical bioflocculation process and a chemical coagulation process in Shanghai, China. 16S rDNA of ammonia-oxidizing bacteria (AOB)was amplified by nested polymerase chain reaction and fingerprinted by denaturing gradient gel electrophoresis for microbial structure analysis. The Shannon diversity index of each sample was determined. The results indicated that the microbial structure of AOB in chemical bioflocculation process was comparable at two operational conditions. The ammonia-oxidizing bacterial communities were similar in three channels of the chemical bioflocculation process and in three serial tanks in the chemical coagulation process at the same condition. The diversity of microbial structures in the chemical bioflocculation process was higher than in the chemical coagulation process, in which the microbial structure was similar to that in the influent. Although the microbial study provides insights to the nitrification removal, higher microbial diversity of AOB does not necessarily mean higher ammonia oxidization. Molecular analysis should be combined with chemical assays to optimize operational conditions.
|
| [43] |
|
| [44] |
|
| [45] |
周琳, 杨柳燕, 蔡友铭, 等. 崇明西红花根际土壤和球茎微生物多样性分析[J]. 核农学报, 2020, 34(11):2452-2459.
为研究崇明西红花栽培地根际土壤和球茎中微生物多样性,采用Illumina MiSeq高通量测序技术对其微生物群落组成进行了比对分析。结果表明,西红花根际土壤和球茎中细菌和真菌在门类水平上菌群类别差异不显著,但在丰富度和多样性方面根际土壤明显高于球茎;在属和种水平上差异显著;在种水平上,根际土壤或球茎均有各自特有的细菌或真菌,且具有较高的相对丰度。西红花致病真菌瓶霉(Phialophora)和背芽突霉(Cadophora)在崇明西红花球茎大量存在。因此,推测西红花病害发生,除与土壤菌群相关外,与其内生细菌和真菌也紧密相关。本研究结果初步分析了崇明栽培地西红花根际土壤和球茎中微生物多样性及群落结构组成,为进一步筛选合适的崇明西红花栽培地土壤和种球杀菌剂提供了理论依据。
|
| [46] |
靳微, 杨预展, 孙海菁, 等. 弗吉尼亚栎母树林外生菌根的真菌多样性[J]. 林业科学, 2020, 56(1):120-132.
|
| [47] |
袁志林, 潘雪玉, 靳微. 林木共生菌系统及其作用机制:以杨树为例[J]. 生态学报, 2019, 39(1):381-397.
|
| [48] |
李敏, 闫伟. 海拔对乌拉山油松根围真菌群落结构的影响[J]. 菌物学报, 2019, 38(11):1992-2006.
|
| [49] |
魏松坡, 宋怡静, 贾黎明, 等. 太行山片麻岩区栓皮栎外生菌根真菌多样性[J]. 菌物学报, 2018, 37(4):422-433.
|
| [50] |
张彤彤, 耿增超, 许晨阳, 等. 秦岭辛家山林区落叶松外生菌根真菌多样性[J]. 微生物学报, 2018, 58(3):443-454.
|
/
| 〈 |
|
〉 |
