Expression characteristics and biological functions of the sex determination gene Bx-sex-1 in Bursaphelenchus xylophilus

WEI Pengfei, LI Yongxia, FENG Yuqian, LIU Zhenkai, ZHANG Xingyao

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (4) : 15-20.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (4) : 15-20. DOI: 10.12302/j.issn.1000-2006.202104004

Expression characteristics and biological functions of the sex determination gene Bx-sex-1 in Bursaphelenchus xylophilus

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Abstract

【Objective】 This study aimed to determine the expression characteristics and biological functions of Bx-sex-1, a sex determination gene in Bursaphelenchus xylophilus. 【Method】 The phylogenetic analysis was performed using the neighbor joining (NJ) method, based on the Jones-Taylor-Thornton (JTT) model in the molecular evolutionary genetic analysis (Mega 6). After collecting pine wood nematodes at different developmental stages using synchronous culture, cDNA was synthesized from the extracted RNA, and the relative expression level of Bx-sex-1 was studied using real-time quantitative PCR (qRT-PCR), where the copy number of Bx-sex-1 in the female and male genomes was analyzed through the absolute quantitative analysis. B. xylophilus adults were socked with double-strand RNA (dsRNA), and the hatching rate and the female to male sex ratio of the progeny were observed. 【Result】 The phylogenetic tree showed that the pine wood nematode Bx-sex-1 was a clade with two other plant parasitic nematodes, and had evolved into a different clade from the free and animal parasitic nematodes, respectively. The Bx-sex-1 gene exhibited the highest relative expression level in the egg period, with the lowest expression in the L2 period that increased gradually up to the adult period. Both female and male genomes had a single copy of the gene. Compared with untreated B. xylophilus, the hatching rate of the eggs and the sex ratio decreased in the experimental samples. 【Conclusion】 The Bx-sex-1 gene of B. xylophilus may play an important role in egg development and sex determination.

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Bursaphelenchus xylophilus / sex determination gene / RNA interference(RNAi) / sex ratio of female to male

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WEI Pengfei , LI Yongxia , FENG Yuqian , et al . Expression characteristics and biological functions of the sex determination gene Bx-sex-1 in Bursaphelenchus xylophilus[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2022, 46(4): 15-20 https://doi.org/10.12302/j.issn.1000-2006.202104004

References

List( Publishing order | Descend order by publishing year | Descend order by cited within ) Chart analysis
[1]
YI C K, BYUN B H, PARK J D, et al. First finding of the pine wood nematode, Bursaphelenchus xylophilus (Steiner & Buhrer) Nickle and its insect vector in Korea[J]. Research Reports of the Forestry Research Institute, 1989(38): 141-149.
Cited in this article [1]
[2]
FUTAI K. Pine wilt in Japan: from first incidence to the present[C]// ZHAO B G, FUTAI K, SUTHERLAND J R, et al. Pine wilt disease. Japan: Springer, 2008:5-12. DOI: 10.1007/978-4-431-75655-2_2.
Cited in this article [1]
[3]
丁晓磊, 张悦, 林司曦, 等. 基于高通量测序技术的松材线虫研究进展[J]. 南京林业大学学报(自然科学版), 2022, 46(4):1-7.
DING X L, ZHANG Y, LIN S X, et al. An overview of high-throughput sequencing techniques applied on Bursaphelenchus xylophilus[J]. J Nanjing For Univ(Nat Sci Ed): 2022, 46(4):1-7.DOI:10.12302/j.issn.1000-2006.202204039.
Cited in this article [1]
[4]
KIKUCHI T, AIKAWA T. Estimation of virulence of Bursaphelenchus xylophilus (Nematoda:Aphelenchoididae) based on its reproductive ability[J]. Nematology, 2007, 9(3): 371-377. DOI:10.1163/156854107781352007.
Cited in this article [2]
[5]
HASEGAWA K, MOTA M M, FUTAI K, et al. Chromosome structure and behaviour in Bursaphelenchus xylophilus (Nematoda: Parasitaphelenchidae) germ cells and early embryo[J]. Nematology, 2006, 8(3): 425-434. DOI:10.1163/156854106778493475.
Cited in this article [3]
[6]
HASEGAWA K, MOTA M, FUTAI K, et al. Developmental biology and cytogenetics of Bursaphelenchus xylophilus[G]//Pine wilt disease: a worldwide threat to forest ecosystems. Dordrecht:Springer, 2008: 91-100.DOI:10.1007/978-1-4020-8455-3_8.
Cited in this article [1]
[7]
KIYOHARA T. Sexual attraction in Bursaphelenchus xylophilus[J]. Jpn J Nematol, 1982, 11:7-12.DOI:10.14855/jjn1972.11.7.
Cited in this article [1]
[8]
GAO M G, LI Y X, ZHANG W, et al. Bx-daf-22 contributes to mate attraction in the gonochoristic nematode Bursaphelenchus xylophilus[J]. Int J Mol Sci, 2019, 20(17): 4316.DOI:10.3390/ijms20174316.
Cited in this article [1]
[9]
BRIDGES C B. Non-disjunction as proof of the chromosome theory of heredity (concluded)[J]. Genetics, 1916, 1(2): 107-163. DOI:10.1093/genetics/1.2.107.
Cited in this article [1]
[10]
MADL J E, HERMAN R K. Polyploids and sex determination in Caenorhabditis elegans[J]. Genetics, 1979, 93(2): 393-402.DOI:10.1093/genetics/93.2.393.
Cited in this article [1]
[11]
GLADDEN J M, FARBOUD B, MEYER B J. Revisiting the X:A signal that specifies Caenorhabditis elegans sexual fate[J]. Genetics, 2007, 177(3):1639-1654. DOI:10.1534/genetics.107.078071.
Cited in this article [3]
[12]
CARMI I, KOPCZYNSKI J B, MEYER B J. The nuclear hormone receptor SEX-1 is an X-chromosome signal that determines nematode sex[J]. Nature, 1998, 396(6707):168-173.DOI:10.1038/24164.
Cited in this article [3]
[13]
ZHOU L F, MA X X, ZHU N J, et al. The role of Mab-3 in spermatogenesis and ontogenesis of pinewood nematode, Bursaphelenchus xylophilus[J]. Pest Manag Sci, 2021, 77(1):138-147.DOI:10.1002/ps.6001.
Cited in this article [1]
[14]
李江梅. 松材线虫tra-1基因克隆及功能分析[D]. 泰安: 山东农业大学, 2019.
LI J M. Cloning and function analysis of tra-1 gene of Bursaphelenchus xylophilus[D]. Tai’an: Shandong Agricultural University, 2019.
Cited in this article [1]
[15]
HIDEAKI I, KAZUYOSHI F. A simple method for disinfection of pine wood nematode eggs using their adhesiveness[J]. Jpn J Nematol, 1985, 15: 64.DOI:10.14855/jjn1972.15.64.
Cited in this article [1]
[16]
FUTAI K, TAKEUCHI Y, SHINYA R. A technique for separating the developmental stages of the propagative form of the pine wood nematode, Bursaphelenchus xylophilus[J]. Nematology, 2009, 11(2):305-307.DOI:10.1163/156854108x399164.
Cited in this article [1]
[17]
牛玉娜. 五指山猪KIT基因拷贝数检测暨猪PSME1基因与免疫性状关系的研究[D]. 武汉: 华中农业大学, 2007.
NIU Y N. The copy number detection of KIT gene in Wuzhishan pig and association analysis between porcine PSME1 gene and immunity traits[D]. Wuhan: Huazhong Agricultural University, 2007.
Cited in this article [1]
[18]
王殿东. 松材线虫休眠相关基因daf-3, daf-9, daf-12, daf-16的克隆及其功能研究[D]. 长春: 吉林大学, 2012.
WANG D D. Cloning and function research of dormancy related gene daf-3, daf-9, daf-12, daf-16 in pine wood nematode, Bursaphelenchus xylophilus[D]. Changchun: Jilin University, 2012.
Cited in this article [1]
[19]
MAMIYA Y. Population increase of Bursaphelenchus lignicolus in wood of pine seedlings in relation with the time after inoculation[J]. Forestry Society, 1974: 249-251.
Cited in this article [1]
[20]
HAAG E S. The evolution of nematode sex determination: C. elegans as a reference point for comparative biology[C]// The C. elegans Research Community. Worm book, 2005.
Cited in this article [1]
[21]
REECE-HOYES J S, SHINGLES J, DUPUY D, et al. Insight into transcription factor gene duplication from Caenorhabditis elegans promoterome-driven expression patterns[J]. BMC Genomics, 2007, 8: 27.DOI:10.1186/1471-2164-8-27.
Cited in this article [1]
[22]
HARGITAI B, KUTNYÁNSZKY V, BLAUWKAMP T A, et al. Xol-1, the master sex-switch gene in C.elegans, is a transcriptional target of the terminal sex-determining factor TRA-1[J]. Development, 2009, 136(23): 3881-3887. DOI:10.1242/dev.034637.
Cited in this article [1]
[23]
CSANKOVSZKI G, COLLETTE K, SPAHL K, et al. Three distinct condensin complexes control C.elegans chromosome dynamics[J]. Curr Biol, 2009, 19(1):9-19.DOI:10.1016/j.cub.2008.12.006.
Cited in this article [1]
[24]
LEHNER B, CALIXTO A, CROMBIE C, et al. Loss of LIN-35, the Caenorhabditis elegans ortholog of the tumor suppressor p105Rb, results in enhanced RNA interference[J]. Genome Biol, 2006, 7(1): R4. DOI:10.1186/gb-2006-7-1-r4.
Cited in this article [1]
[25]
HAAG E S, WANG S P, KIMBLE J. Rapid coevolution of the nematode sex-determining genes fem-3 and tra-2[J]. Curr Biol, 2002, 12(23):2035-2041. DOI:10.1016/s0960-9822(02)01333-7.
Cited in this article [1]
[26]
HAAG E S, KIMBLE J. Regulatory elements required for development of Caenorhabditis elegans hermaphrodites are conserved in the tra-2 homologue of C. remanei, a male/female sister species[J]. Genetics, 2000, 155(1): 105-116.DOI:10.1093/genetics/155.1.105.
Cited in this article [1]
[27]
KIKUCHI T, COTTON J A, DALZELL J J, et al. Genomic insights into the origin of parasitism in the emerging plant pathogen Bursaphelenchus xylophilus[J]. PLoS Pathog, 2011, 7(9): e1002219.DOI:10.1371/journal.ppat.1002219.
Cited in this article [1]
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