Study on ploidy and genome sizes of Osmanthus fragrans and its related species

DUAN Yifan, LI Lan, YANG Xinxin, WANG Xianrong, ZHANG Min, ZHANG Cheng, CHAI Zihan

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (5) : 47-52.

PDF(2002 KB)
南京林业大学学报（自然科学版）
PDF(2002 KB)
JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (5) : 47-52. DOI: 10.12302/j.issn.1000-2006.202104027

Study on ploidy and genome sizes of Osmanthus fragrans and its related species

Author information +
History +

Abstract

【Objective】 This study aimed to provide new cytological evidence for the classification of Osmanthus fragrans and its related species (O. fortunei, O. heterophyllus and O. cooperi).【Method】 In this study, we used representative samples of Oryza sativa ‘Nipponbare’ to estimate the ploidy and genome sizes of 14 O. fragrans cultivars and three related species by using flow cytometry.【Result】 Average values of the O. fragrans Asiaticus Group, Luteus Group, Albus Group and Aurantiacus Group are 777.24, 760.85, 739.06 and 717.29 Mb, respectively. The largest genome size of the cultivar O. fragrans ‘Siji’ was 903.14 Mb. Genome sizes of O. cooperi and O. heterophyllus are 817.19 and 769.50 Mb, respectively; O. fortunei has the smallest genome size (729.11 Mb).【Conclusion】 Fourteen O. fragrans cultivars and three related species were diploid, but their genome sizes differed. The genome size of O. cooperi was close to that of O. fragrans ‘Xionghuang’, O. fragrans ‘Jinqiu’ and O. fragrans ‘Yuntian Cai.’ These results indicated a close relationship between O. cooperi and O. fragrans.

Key words

Osmanthus fragrans / close relationship / flow cytometry / ploidy identification / genome size

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations
DUAN Yifan , LI Lan , YANG Xinxin , et al . Study on ploidy and genome sizes of Osmanthus fragrans and its related species[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2021, 45(5): 47-52 https://doi.org/10.12302/j.issn.1000-2006.202104027

References

List( Publishing order | Descend order by publishing year | Descend order by cited within ) Chart analysis
[1]
尚富德, 韩远记, 袁王俊, 等. 木犀属及桂花品种分类研究进展[J]. 河南大学学报(自然科学版), 2012, 42(5):608-612.
SHANG F D, HAN Y J, YUAN W J, et al. Review on classification of Osmanthus and Osmanthus fragrans cultivars[J]. J Henan Univ (Nat Sci), 2012, 42(5):608-612.DOI: 10.15991/j.cnki.411100.2012.05.015.
https://doi.org/10.15991/j.cnki.411100.2012.05.015
Cited in this article [1]
[2]
向其柏, 刘玉莲. 中国桂花品种图志[M]. 杭州: 浙江科学技术出版社, 2008.
XIANG Q B, LIU Y L. Illustrated monograph of the sweet Osmanthus fragrans cultivars in China[M]. Hangzhou: Zhejiang Science & Technology Press, 2008.
Cited in this article [1]
[3]
陆瑞林. 中国植物志[M]. 北京: 科学出版社, 1992, 61:85-112.
LU R L. Flora of China. Beijing: Science Press, 1992, 61:85-112.
Cited in this article [2]
[4]
季春峰. 木犀属(Osmanthus)的系统分类学研究[D]. 南京:南京林业大学, 2004.
JI C F. Systematic taxonomy of the genus Osmanthus (Oleaceae)[D]. Nanjing:Nanjing Forestry University, 2004.
Cited in this article [1]
[5]
LOUREIRO J. Flora cochin chinensis[M]. Ulyssipone: Typis,et Expensis Academicis,1790.
Cited in this article [1]
[6]
胡绍庆, 张后勇, 吴光洪, 等. 桂花品种分类系统研究[J]. 浙江大学学报(农业与生命科学版), 2005, 31(4):445-448.
HU S Q, ZHANG H Y, WU G H, et al. Studies on resource and taxon of Osmanthus fagrans cultivars[J]. J Zhejiang Univ (Agric& Life Sci), 2005, 31(4):445-448.DOI: 10.3321/j.issn:1008-9209.2005.04.015.
https://doi.org/10.3321/j.issn:1008-9209.2005.04.015
Cited in this article [1]
[7]
郭世权. 木犀属分子系统学研究[D]. 江西: 江西农业大学, 2009.
GUO S Q. Molecular phylogeny of the genus Osmanthus[D]. Jiangxi: Jiangxi Agricultural University, 2009.
Cited in this article [1]
[8]
CHANG M C, QIU L Q, WEI Z, et al. Flora of China: vol. 15[M]. Beijing: Science Press; St. Louis, Beijing & Missouri Botanical Garden Press: 286-292.
Cited in this article [1]
[9]
许炳强, 夏念和, 王少平, 等. 中国木犀属植物叶表皮形态[J]. 武汉植物学研究, 2007, 25(1):1-10.
XU B Q, XIA N H, WANG S P, et al. Leaf epidermal morphology of Osmanthus(Oleaceae) from China[J]. J Wuhan Bot Res, 2007, 25(1):1-10.DOI: 10.3969/j.issn.2095-0837.2007.01.001.
https://doi.org/10.3969/j.issn.2095-0837.2007.01.001
Cited in this article [1]
[10]
卢龙斗, 常生辉, 高武军, 等. 木犀属植物叶片微形态结构[J]. 南京林业大学学报(自然科学版), 2008, 32(6):52-56.
LU L D, CHANG S H, GAO W J, et al. The structure of leaf micromorphology of Osmanthus[J]. J Nanjing For Univ (Nat Sci Ed), 2008, 32(6):52-56.DOI: 10.3969/j.issn.1000-2006.2008.06.012.
https://doi.org/10.3969/j.issn.1000-2006.2008.06.012
Cited in this article [1]
[11]
常生辉, 卢龙斗, 高武军, 等. 木犀属植物叶柄的比较解剖学研究[J]. 热带亚热带植物学报, 2008, 16(1):10-18.
CHANG S H, LU L D, GAO W J, et al. Comparative anatomical studies on petioles of Osmanthus[J]. J Trop Subtrop Bot, 2008, 16(1):10-18.DOI: 10.3969/j.issn.1005-3395.2008.01.002.
https://doi.org/10.3969/j.issn.1005-3395.2008.01.002
Cited in this article [1]
[12]
徐沂春. 桂花遗传多样性及不同性别花的花芽特性[D]. 杭州:浙江农林大学, 2013.
XU Y C. The genetic diversity and flower bud characteristics of different sex in Osmanthus fragrans[D]. Hangzhou:Zhejiang A & F University, 2013.
Cited in this article [1]
[13]
TAYLOR H. Cyto-taxonomy and phylogeny of the Oleaceae[J]. Brittonia, 1945, 5(4):337-367.DOI: 10.2307/2804889.
https://doi.org/10.2307/2804889
http://link.springer.com/10.2307/2804889
Cited in this article [3]
[14]
李玉阁, 袁王俊, 董美芳, 等. 桂花的核型研究[J]. 南京林业大学学报(自然科学版), 2004, 28(S1):87-90.
LI Y G, YUAN W J, DONG M F, et al. A study on karyotype of Osmanthus fragrans Lour.[J]. J Nanjing For Univ (Nat Sci Ed), 2004, 28(S1):87-90.DOI: 10.3969/j.issn.1000-2006.2004.z1.014.
https://doi.org/10.3969/j.issn.1000-2006.2004.z1.014
Cited in this article [1]
[15]
MIYASHITA T, ARAKI H, HOSHINO Y. Ploidy distribution and DNA content variations of Lonicera caerulea (Caprifoliaceae) in Japan[J]. J Plant Res, 2011, 124(1):1-9.DOI: 10.1007/s10265-010-0341-6.
https://doi.org/10.1007/s10265-010-0341-6
http://link.springer.com/10.1007/s10265-010-0341-6
Cited in this article [1]
[16]
ALONSO C, PÉREZ R, BAZAGA P, et al. Global DNA cytosine methylation as an evolving trait:phylogenetic signal and correlated evolution with genome size in angiosperms[J]. Front Genet, 2015, 6:4.DOI: 10.3389/fgene.2015.00004.
https://doi.org/10.3389/fgene.2015.00004
Cited in this article [1]
[17]
GROTKOPP E, REJMÁNEK,SANDERSON M J, et al. Evolution of genome size in pines (Pinus) and its life-history correlates:supertree analyses[J]. Evolution, 2004, 58(8):1705-1729.DOI: 10.1111/j.0014-3820.2004.tb00456.x.
https://doi.org/10.1111/j.0014-3820.2004.tb00456.x
http://www.blackwell-synergy.com/toc/evo/58/8
Cited in this article [1]
[18]
田新民, 周香艳, 弓娜. 流式细胞术在植物学研究中的应用:检测植物核DNA含量和倍性水平[J]. 中国农学通报, 2011, 27(9):21-27.
TIAN X M, ZHOU X Y, GONG N. Applications of flow cytometry in plant research: analysis of nuclear DNA content and ploidy level in plant cells[J]. Chin Agric Sci Bull, 2011, 27(9):21-27.
Cited in this article [2]
[19]
DOLEZEL J, GREILHUBER J, SUDA J. Estimation of nuclear DNA content in plants using flow cytometry[J]. Nat Protoc, 2007, 2(9):2233-2244.DOI: 10.1038/nprot.2007.310.
https://doi.org/10.1038/nprot.2007.310
https://doi.org/10.1038/nprot.2007.310
Cited in this article [1]
[20]
GALBRAITH D W, HARKINS K R, MADDOX J M, et al. Rapid flow cytometric analysis of the cell cycle in intact plant tissues[J]. Science, 1983, 220(4601):1049-1051.DOI: 10.1126/science.220.4601.1049.
https://doi.org/10.1126/science.220.4601.1049
https://www.sciencemag.org/lookup/doi/10.1126/science.220.4601.1049
Cited in this article [1]
[21]
潘跃芝, 张亦弛, 龚洵, 等. 4种人参属植物基因组大小的测定[J]. 植物分类与资源学报, 2014, 36(2):233-236.
PAN Y Z, ZHANG Y C, GONG X, et al. Estimation of genome size of four Panax species by flow cytometry[J]. Plant Divers Resour, 2014, 36(2):233-236.
Cited in this article [1]
[22]
LEITCH IJ, JOHNSTON E, PELLICER J, et al. Plant DNA C-values database release 7.1, April 2019[DB/OL].( 2019-04)[2020-06-07]. https://cvalues.science.kew.org/.
https://cvalues.science.kew.org/.
Cited in this article [1]
[23]
LEITCH I J, SOLTIS D E, SOLTIS P S, et al. Evolution of DNA amounts across land plants (embryophyta)[J]. Ann Bot, 2005, 95(1):207-217.DOI: 10.1093/aob/mci014.
https://doi.org/10.1093/aob/mci014
https://academic.oup.com/aob/article-lookup/doi/10.1093/aob/mci014
Cited in this article [1]
[24]
李祯. 樱属部分种倍性及基因组大小研究[D]. 南京:南京林业大学, 2015.
LI Z. Estimation of ploidy and genome size on several species in Cerasus[D]. Nanjing:Nanjing Forestry University, 2015.
Cited in this article [1]
[25]
韩莎, 李四军, 许晓东, 等. 利用流式细胞仪快速鉴定桑树细胞的染色体倍数性[J]. 蚕业科学, 2013, 39(6):1042-1048.
HAN S, LI S J, XU X D, et al. Quick identification of chromosomal ploidy of mulberry cells by flow cytometry[J]. Sci Seric, 2013, 39(6):1042-1048.DOI: 10.13441/j.cnki.cykx.2013.06.01.
https://doi.org/10.13441/j.cnki.cykx.2013.06.01
Cited in this article [1]
[26]
王荣邦, 张秀海, 吴忠义, 等. 菊花染色体倍性鉴定研究[J]. 安徽农业科学, 2010, 38(23):12778-12780,12789.
WANG R B, ZHANG X H, WU Z Y, et al. Common methods of cell chromosome ploidy identification for Chrysanthemum and its application in breeding[J]. J Anhui Agric Sci, 2010, 38(23):12778-12780,12789.DOI: 10.13989/j.cnki.0517-6611.2010.23.220.
https://doi.org/10.13989/j.cnki.0517-6611.2010.23.220
Cited in this article [1]
[27]
周浩. 六倍体小麦形成早期基因表达变化研究[D]. 长春:东北师范大学, 2015.
ZHOU H. Gene expression changes in early stages of allohexaploid wheat formation[D]. Changchun:Northeast Normal University, 2015.
Cited in this article [1]
[28]
刘幼琪, 刘信民, 陈建国, 等. 水稻籼粳杂种多倍体的研究[J]. 湖北大学学报(自然科学版), 2002, 24(1):72-76.
LIU Y Q, LIU X M, CHEN J G, et al. Studies on somatic chimaera in polyploid of indica × japonica hybrids induced by colchicine[J]. J Hubei Univ (Nat Sci Ed), 2002, 24(1):72-76.DOI: 10.3969/j.issn.1000-2375.2002.01.018.
https://doi.org/10.3969/j.issn.1000-2375.2002.01.018
Cited in this article [1]
[29]
BENNETT M. Nuclear DNA amounts in angiosperms[J]. Ann Bot, 1995, 76(2):113-176.DOI: 10.1006/anbo.1995.1085.
https://doi.org/10.1006/anbo.1995.1085
https://academic.oup.com/aob/article-lookup/doi/10.1006/anbo.1995.1085
Cited in this article [1]
[30]
吴丽萍, 唐岩, 李颖岳, 等. 枣和酸枣基因组大小测定[J]. 北京林业大学学报, 2013, 35(3):77-83.
WU L P, TANG Y, LI Y Y, et al. Estimation of genome size of Ziziphus jujuba and Z. acdiojujuba[J]. J Beijing For Univ, 2013, 35(3):77-83.DOI: 10.13332/j.1000-1522.2013.03.012.
https://doi.org/10.13332/j.1000-1522.2013.03.012
Cited in this article [1]

南京林业大学现代分析测试中心林峰老师在实验过程中给予帮助与支持;江苏省农业科学院及常州市芳芝林现代农业产业园区提供实验材料。

RIGHTS & PERMISSIONS

Copyright reserved © 2021.
PDF(2002 KB)

Accesses

Citation

Detail
Sections
Recommended
The full text is translated into English by AI, aiming to facilitate reading and comprehension. The core content is subject to the explanation in Chinese.

Author

Reader

Reviewer

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

/

〈 〉