Study on ploidy and genome sizes of Osmanthus fragrans and its related species

doi:10.12302/j.issn.1000-2006.202104027

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (5): 47-52.doi: 10.12302/j.issn.1000-2006.202104027

Previous Articles Next Articles

Study on ploidy and genome sizes of Osmanthus fragrans and its related species

DUAN Yifan¹^,²(), LI Lan¹, YANG Xinxin¹, WANG Xianrong¹^,², ZHANG Min¹^,², ZHANG Cheng¹, CHAI Zihan¹

1. Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, China
2. Cangnan Forestry Bureau of Zhejiang Province, Cangnan 325800,China

Received:2021-04-18 Accepted:2021-06-10 Online:2021-09-30 Published:2021-09-30

Abstract

Abstract:

【Objective】 This study aimed to provide new cytological evidence for the classification of Osmanthus fragrans and its related species (O. fortunei, O. heterophyllus and O. cooperi).【Method】 In this study, we used representative samples of Oryza sativa ‘Nipponbare’ to estimate the ploidy and genome sizes of 14 O. fragrans cultivars and three related species by using flow cytometry.【Result】 Average values of the O. fragrans Asiaticus Group, Luteus Group, Albus Group and Aurantiacus Group are 777.24, 760.85, 739.06 and 717.29 Mb, respectively. The largest genome size of the cultivar O. fragrans ‘Siji’ was 903.14 Mb. Genome sizes of O. cooperi and O. heterophyllus are 817.19 and 769.50 Mb, respectively; O. fortunei has the smallest genome size (729.11 Mb).【Conclusion】 Fourteen O. fragrans cultivars and three related species were diploid, but their genome sizes differed. The genome size of O. cooperi was close to that of O. fragrans ‘Xionghuang’, O. fragrans ‘Jinqiu’ and O. fragrans ‘Yuntian Cai.’ These results indicated a close relationship between O. cooperi and O. fragrans.

Key words: Osmanthus fragrans, close relationship, flow cytometry, ploidy identification, genome size

CLC Number:

S685
S718

DUAN Yifan, LI Lan, YANG Xinxin, WANG Xianrong, ZHANG Min, ZHANG Cheng, CHAI Zihan. Study on ploidy and genome sizes of Osmanthus fragrans and its related species[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(5): 47-52.

Figures/Tables 4

Table 1

Fig.1

Fig.2

Table 2

References 30

[1]	尚富德, 韩远记, 袁王俊, 等. 木犀属及桂花品种分类研究进展[J]. 河南大学学报(自然科学版), 2012, 42(5):608-612.
	SHANG F D, HAN Y J, YUAN W J, et al. Review on classification of Osmanthus and Osmanthus fragrans cultivars[J]. J Henan Univ (Nat Sci), 2012, 42(5):608-612.DOI: 10.15991/j.cnki.411100.2012.05.015. doi: 10.15991/j.cnki.411100.2012.05.015
[2]	向其柏, 刘玉莲. 中国桂花品种图志[M]. 杭州: 浙江科学技术出版社, 2008.
	XIANG Q B, LIU Y L. Illustrated monograph of the sweet Osmanthus fragrans cultivars in China[M]. Hangzhou: Zhejiang Science & Technology Press, 2008.
[3]	陆瑞林. 中国植物志[M]. 北京: 科学出版社, 1992, 61:85-112.
	LU R L. Flora of China. Beijing: Science Press, 1992, 61:85-112.
[4]	季春峰. 木犀属(Osmanthus)的系统分类学研究[D]. 南京:南京林业大学, 2004.
	JI C F. Systematic taxonomy of the genus Osmanthus (Oleaceae)[D]. Nanjing:Nanjing Forestry University, 2004.
[5]	LOUREIRO J. Flora cochin chinensis[M]. Ulyssipone: Typis,et Expensis Academicis,1790.
[6]	胡绍庆, 张后勇, 吴光洪, 等. 桂花品种分类系统研究[J]. 浙江大学学报(农业与生命科学版), 2005, 31(4):445-448.
	HU S Q, ZHANG H Y, WU G H, et al. Studies on resource and taxon of Osmanthus fagrans cultivars[J]. J Zhejiang Univ (Agric& Life Sci), 2005, 31(4):445-448.DOI: 10.3321/j.issn:1008-9209.2005.04.015. doi: 10.3321/j.issn:1008-9209.2005.04.015
[7]	郭世权. 木犀属分子系统学研究[D]. 江西: 江西农业大学, 2009.
	GUO S Q. Molecular phylogeny of the genus Osmanthus[D]. Jiangxi: Jiangxi Agricultural University, 2009.
[8]	CHANG M C, QIU L Q, WEI Z, et al. Flora of China: vol. 15[M]. Beijing: Science Press; St. Louis, Beijing & Missouri Botanical Garden Press: 286-292.
[9]	许炳强, 夏念和, 王少平, 等. 中国木犀属植物叶表皮形态[J]. 武汉植物学研究, 2007, 25(1):1-10.
	XU B Q, XIA N H, WANG S P, et al. Leaf epidermal morphology of Osmanthus(Oleaceae) from China[J]. J Wuhan Bot Res, 2007, 25(1):1-10.DOI: 10.3969/j.issn.2095-0837.2007.01.001. doi: 10.3969/j.issn.2095-0837.2007.01.001
[10]	卢龙斗, 常生辉, 高武军, 等. 木犀属植物叶片微形态结构[J]. 南京林业大学学报(自然科学版), 2008, 32(6):52-56.
	LU L D, CHANG S H, GAO W J, et al. The structure of leaf micromorphology of Osmanthus[J]. J Nanjing For Univ (Nat Sci Ed), 2008, 32(6):52-56.DOI: 10.3969/j.issn.1000-2006.2008.06.012. doi: 10.3969/j.issn.1000-2006.2008.06.012
[11]	常生辉, 卢龙斗, 高武军, 等. 木犀属植物叶柄的比较解剖学研究[J]. 热带亚热带植物学报, 2008, 16(1):10-18.
	CHANG S H, LU L D, GAO W J, et al. Comparative anatomical studies on petioles of Osmanthus[J]. J Trop Subtrop Bot, 2008, 16(1):10-18.DOI: 10.3969/j.issn.1005-3395.2008.01.002. doi: 10.3969/j.issn.1005-3395.2008.01.002
[12]	徐沂春. 桂花遗传多样性及不同性别花的花芽特性[D]. 杭州:浙江农林大学, 2013.
	XU Y C. The genetic diversity and flower bud characteristics of different sex in Osmanthus fragrans[D]. Hangzhou:Zhejiang A & F University, 2013.
[13]	TAYLOR H. Cyto-taxonomy and phylogeny of the Oleaceae[J]. Brittonia, 1945, 5(4):337-367.DOI: 10.2307/2804889. doi: 10.2307/2804889
[14]	李玉阁, 袁王俊, 董美芳, 等. 桂花的核型研究[J]. 南京林业大学学报(自然科学版), 2004, 28(S1):87-90.
	LI Y G, YUAN W J, DONG M F, et al. A study on karyotype of Osmanthus fragrans Lour.[J]. J Nanjing For Univ (Nat Sci Ed), 2004, 28(S1):87-90.DOI: 10.3969/j.issn.1000-2006.2004.z1.014. doi: 10.3969/j.issn.1000-2006.2004.z1.014
[15]	MIYASHITA T, ARAKI H, HOSHINO Y. Ploidy distribution and DNA content variations of Lonicera caerulea (Caprifoliaceae) in Japan[J]. J Plant Res, 2011, 124(1):1-9.DOI: 10.1007/s10265-010-0341-6. doi: 10.1007/s10265-010-0341-6
[16]	ALONSO C, PÉREZ R, BAZAGA P, et al. Global DNA cytosine methylation as an evolving trait:phylogenetic signal and correlated evolution with genome size in angiosperms[J]. Front Genet, 2015, 6:4.DOI: 10.3389/fgene.2015.00004. doi: 10.3389/fgene.2015.00004
[17]	GROTKOPP E, REJMÁNEK,SANDERSON M J, et al. Evolution of genome size in pines (Pinus) and its life-history correlates:supertree analyses[J]. Evolution, 2004, 58(8):1705-1729.DOI: 10.1111/j.0014-3820.2004.tb00456.x. doi: 10.1111/j.0014-3820.2004.tb00456.x
[18]	田新民, 周香艳, 弓娜. 流式细胞术在植物学研究中的应用:检测植物核DNA含量和倍性水平[J]. 中国农学通报, 2011, 27(9):21-27.
	TIAN X M, ZHOU X Y, GONG N. Applications of flow cytometry in plant research: analysis of nuclear DNA content and ploidy level in plant cells[J]. Chin Agric Sci Bull, 2011, 27(9):21-27.
[19]	DOLEZEL J, GREILHUBER J, SUDA J. Estimation of nuclear DNA content in plants using flow cytometry[J]. Nat Protoc, 2007, 2(9):2233-2244.DOI: 10.1038/nprot.2007.310. doi: 10.1038/nprot.2007.310
[20]	GALBRAITH D W, HARKINS K R, MADDOX J M, et al. Rapid flow cytometric analysis of the cell cycle in intact plant tissues[J]. Science, 1983, 220(4601):1049-1051.DOI: 10.1126/science.220.4601.1049. doi: 10.1126/science.220.4601.1049
[21]	潘跃芝, 张亦弛, 龚洵, 等. 4种人参属植物基因组大小的测定[J]. 植物分类与资源学报, 2014, 36(2):233-236.
	PAN Y Z, ZHANG Y C, GONG X, et al. Estimation of genome size of four Panax species by flow cytometry[J]. Plant Divers Resour, 2014, 36(2):233-236.
[22]	LEITCH IJ, JOHNSTON E, PELLICER J, et al. Plant DNA C-values database release 7.1, April 2019[DB/OL].( 2019-04)[2020-06-07]. https://cvalues.science.kew.org/.
[23]	LEITCH I J, SOLTIS D E, SOLTIS P S, et al. Evolution of DNA amounts across land plants (embryophyta)[J]. Ann Bot, 2005, 95(1):207-217.DOI: 10.1093/aob/mci014. doi: 10.1093/aob/mci014
[24]	李祯. 樱属部分种倍性及基因组大小研究[D]. 南京:南京林业大学, 2015.
	LI Z. Estimation of ploidy and genome size on several species in Cerasus[D]. Nanjing:Nanjing Forestry University, 2015.
[25]	韩莎, 李四军, 许晓东, 等. 利用流式细胞仪快速鉴定桑树细胞的染色体倍数性[J]. 蚕业科学, 2013, 39(6):1042-1048.
	HAN S, LI S J, XU X D, et al. Quick identification of chromosomal ploidy of mulberry cells by flow cytometry[J]. Sci Seric, 2013, 39(6):1042-1048.DOI: 10.13441/j.cnki.cykx.2013.06.01. doi: 10.13441/j.cnki.cykx.2013.06.01
[26]	王荣邦, 张秀海, 吴忠义, 等. 菊花染色体倍性鉴定研究[J]. 安徽农业科学, 2010, 38(23):12778-12780,12789.
	WANG R B, ZHANG X H, WU Z Y, et al. Common methods of cell chromosome ploidy identification for Chrysanthemum and its application in breeding[J]. J Anhui Agric Sci, 2010, 38(23):12778-12780,12789.DOI: 10.13989/j.cnki.0517-6611.2010.23.220. doi: 10.13989/j.cnki.0517-6611.2010.23.220
[27]	周浩. 六倍体小麦形成早期基因表达变化研究[D]. 长春:东北师范大学, 2015.
	ZHOU H. Gene expression changes in early stages of allohexaploid wheat formation[D]. Changchun:Northeast Normal University, 2015.
[28]	刘幼琪, 刘信民, 陈建国, 等. 水稻籼粳杂种多倍体的研究[J]. 湖北大学学报(自然科学版), 2002, 24(1):72-76.
	LIU Y Q, LIU X M, CHEN J G, et al. Studies on somatic chimaera in polyploid of indica × japonica hybrids induced by colchicine[J]. J Hubei Univ (Nat Sci Ed), 2002, 24(1):72-76.DOI: 10.3969/j.issn.1000-2375.2002.01.018. doi: 10.3969/j.issn.1000-2375.2002.01.018
[29]	BENNETT M. Nuclear DNA amounts in angiosperms[J]. Ann Bot, 1995, 76(2):113-176.DOI: 10.1006/anbo.1995.1085. doi: 10.1006/anbo.1995.1085
[30]	吴丽萍, 唐岩, 李颖岳, 等. 枣和酸枣基因组大小测定[J]. 北京林业大学学报, 2013, 35(3):77-83.
	WU L P, TANG Y, LI Y Y, et al. Estimation of genome size of Ziziphus jujuba and Z. acdiojujuba[J]. J Beijing For Univ, 2013, 35(3):77-83.DOI: 10.13332/j.1000-1522.2013.03.012. doi: 10.13332/j.1000-1522.2013.03.012

Related Articles 15

[1]	SONG Ziqi, BIAN Guoliang, LIN Feng, HU Fengrong, SHANG Xulan. Establishment and application of a flow cytometry method for chromosome ploidy identification of Cyclocarya paliurus [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(2): 61-68.
[2]	LIU Jialei, BAI Run’e, ZHANG Kai, WEN Caiyi, YAN Fengming. Description of common whiteflies (Hemiptera: Aleyrodidae) on Osmanthus fragrans in China [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(5): 237-244.
[3]	QIU Jing, LI Jiabao, ZHU Dahai, CHEN Xin. Taxonomic implications of genome sizes and micromorphological characteristics of leaf epidermis of species in Sorbus Sect. Alnifoliae [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(3): 77-86.
[4]	WANG Lianggui, PAN Duo, DING Huifen, SHI Tingting, YUE Yuanzheng, YANG Xiulian. Osmanthus fragrans ‘Nanlin Caiyun’:a new cultivar of Osmanthus [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(2): 243-244.
[5]	CUI Qi, WU Yun, LI Dongze, WU Fan, HAN Ruilian, HUANG Junhua, HU Shaoqing. Changes of coloration and pigment compositions during leaf development of Osmanthus fragrans colour group cultivar [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(2): 79-86.
[6]	MA Qiuyue, WANG Yuxiao, LI Qianzhong, LI Shushun, WEN Jing, ZHU Lu, YAN Kunyuan, DU Yiming, XIE Zhijun, LI Shuxian, OUYANG Fangqun, LU Chengdai. Estimation of genome sizes of six Acer species by flow cytometry and K-mer analysis [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(1): 163-170.
[7]	ZHANG Cheng, WANG Xiaoyan, WANG Xianrong, DUAN Yifan, ZHANG Min, SHI Dawei, ZHU Yue, SONG Yanfeng, CHAI Zihan, LI Lan. Photosynthesis and hormone study of male and hermaphroditic Osmanthus fragrans at different flowering stages [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(5): 75-80.
[8]	KANG Hongxing, TIAN Qingyin, SHI Tingting, YUE Yuanzheng, YANG Xiulian, WANG Lianggui. Osmanthus fragrans ‘Nanlin Caijin’: a new color-leaf cultivar of Osmanthus [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(4): 247-248.
[9]	YANG Xuilian, ZAI Zhouying, SHI Tingting, YUE Yuanzheng, WANG Lianggui. Osmanthus fragrans ‘Nanlin Caiyu’: a new color-leaf cultivar of Osmanthus [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(3): 243-244.
[10]	ZANG Dekui, MA Yan, WANG Yanling, BAO Jian, BAO Wei, BAO Zhixian. Osmanthus fragrans ‘Jincan’: a new cultivar of O. fragrans [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(2): 241-242.
[11]	LI Chengcheng, WU Qichao, BAO Jian, BAO Wei, BAO Zhixian, ZANG Dekui, MA Yan. Osmanthus fragrans ‘Congyu’: a new cultivar of Osmanthus fragrans [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(1): 241-242.
[12]	WU Qichao, MA Yan, LI Chengcheng, XU Jianjun, YAO Yanjun, ZANG Dekui. Osmanthus fragrans ‘Dongrong’: a new cultivar of osmanthus [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(3): 245-246.
[13]	FENG Yuanyuan, LI Qingying, HUANG Junhua, HU Shaoqing. Numerical classification of 25 color-leafed Osmanthus fragrans clones (cultivars) [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(1): 107-115.
[14]	LI Weiqiang, DAI Xiaogang, LI Xiaoping, LI Shuxian. Assessment of ploidy levels in the Populus deltoides Marsh. germplasm [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2019, 43(5): 51-58.
[15]	ZHENG Sunyuan, ZHU Hong, JIN Siyu, WANG Mengjuan, SUN Jie, GU Chengcheng, PEI Yinwei, WANG Xianrong, DUAN Yifan. Environment-dependent phenotypic variation of Osmanthus fragrans [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2019, 43(02): 38-46.

Metrics

Comments

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

材料 materials	采集地 location	凭证标本编号 voucher specimen
‘四季’O. fragrans ‘Siji’	南京林业大学校内	SJG-01
‘小叶佛顶珠’O. fragrans ‘Xiaoye Fodingzhu’	常州市芳芝林现代农业产业园区	SJG-02
‘天香台阁’O. fragrans ‘Tianxiang Taige’	常州市芳芝林现代农业产业园区	SJG-03
‘雄黄’O. fragrans ‘Xionghuang’	南京林业大学校内	DG-01
‘蒲城丹’O. fragrans ‘Pucheng Dan’	常州市芳芝林现代农业产业园区	DG-02
‘齿丹’O. fragrans ‘Chi Dan’	常州市芳芝林现代农业产业园区	DG-03
‘金球’O. fragrans ‘Jinqiu’	南京林业大学校内	JG-01
‘波叶金’O. fragrans ‘Boye Jin’	南京林业大学校内	JG-02
‘速生金’O. fragrans ‘Susheng Jin’	常州市芳芝林现代农业产业园区	JG-03
‘早黄’O. fragrans ‘Zao Huang’	常州市芳芝林现代农业产业园区	YG-01
‘早银’O. fragrans ‘Zao Yin’	南京林业大学校内	YG-02
‘云田彩’O. fragrans ‘Yuntian Cai’	常州市芳芝林现代农业产业园区	CYG-01
‘虔南桂妃’O. fragrans ‘Qiannan Guifei’	常州市芳芝林现代农业产业园区	CYG-02
‘银碧双辉’O. fragrans ‘Yinbi Shuanghui’	常州市芳芝林现代农业产业园区	CYG-03
齿叶木犀O. × fortunei	常州市芳芝林现代农业产业园区	SPE-01
柊树O. heterophyllus	常州市芳芝林现代农业产业园区	SPE-02
华东木犀O. cooperi	常州市芳芝林现代农业产业园区	SPE-03

样品 samples	样品峰值(B) peak value of samples	水稻峰值(A) peak value of Oryza sativa	样品峰值/ 水稻峰值 ratio of B to A	样品估计基因组大小/Mb genome size	C值/pg C-value
‘四季’O. fragrans ‘Siji’	17 010	7 907	2.151	903.14 ± 3.87 m	0.92
‘小叶佛顶珠’O. fragrans ‘Xiaoye Fodingzhu’	25 136	15 097	1.665	697.55 ±6.11 c	0.71
‘天香台阁’O. fragrans ‘Tianxiang Taige’	19 882	11 438	1.738	731.04 ± 4.60 d	0.74
‘雄黄’O. fragrans ‘Xionghuang’	22 749	11 931	1.907	801.15 ± 2.12 i	0.82
‘蒲城丹’O. fragrans ‘Pucheng Dan’	16 607	10 586	1.569	657.97 ± 2.09 a	0.67
‘齿丹’O. fragrans ‘Chi Dan’	16 708	10 169	1.643	692.74 ± 2.53 bc	0.71
‘金球’O. fragrans ‘Jinqiu’	20 232	10 496	1.928	808.57 ± 4.45 j	0.83
‘波叶金’O. fragrans ‘Boye Jin’	22 276	11 891	1.873	787.10 ± 2.16 h	0.80
‘速生金’O. fragrans ‘Susheng Jin’	16 641	10 180	1.635	686.87 ± 1.10 b	0.70
‘早黄’O. fragrans ‘Zao Huang’	15 815	9 503	1.664	697.86 ± 2.92 c	0.71
‘早银’O. fragrans ‘Zao Yin’	19 820	10 655	1.860	780.25 ± 1.50 g	0.80
‘云田彩’O. fragrans ‘Yuntian Cai’	21 674	10 943	1.981	832.79 ± 2.26 l	0.85
‘虔南桂妃’O. fragrans ‘Qiannan Guifei’	18 655	10 247	1.821	765.31 ± 1.79 f	0.78
‘银碧双辉’O. fragrans ‘Yinbi Shuanghui’	16 061	9 032	1.778	747.19 ±1.30 e	0.76
齿叶木犀O. × fortunei	16 377	9 435	1.736	729.11 ± 2.73 d	0.74
柊树O. heterophyllus	20 118	10 977	1.832	769.50 ±3.12 f	0.79
华东木犀O. cooperi	19 058	9 788	1.947	817.19 ± 1.96 k	0.84

Study on ploidy and genome sizes of Osmanthus fragrans and its related species

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 4

References 30

Related Articles 15

Recommended Articles

Metrics

Comments

Author

Reader

Reviewer