Th2CysPrx gene enhanced abiotic stress tolerances of Saccharomyces cerevisiae

WANG Yuanyuan, LIU Baichao, JIANG Bo, WANG Danni, GAO Caiqiu

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (4) : 87-94.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (4) : 87-94. DOI: 10.12302/j.issn.1000-2006.202105023

Th2CysPrx gene enhanced abiotic stress tolerances of Saccharomyces cerevisiae

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Abstract

【Objective】 Peroxidoxin is an important protein in organisms for regulation of the redox system in plants, and plays an important role in plant growth and metabolism. Previous research has found that overexpression of the Th2CysPrx gene increased the expression levels of four antioxidant genes (ThGSTZ1, ThGPX, ThSOD and ThPOD), thereby increasing the NaCl stress tolerance of transgenic Arabidopsis and Tamarix hispida. This study explored whether the Th2CysPrx gene is involved in the response to other salts or stresses in addition to NaCl stress. 【Method】 Th2CysPrx was constructed into the pYES2 yeast expression vector and transformed into the INVSC1 cells of Saccharomyces cerevisiae. The cell growth between the recombinant vector and empty vector was compared after applying various abiotic stresses. 【Result】 The survival rate of cells with the overexpressed Th2CysPrx gene was not significantly different from that of the control under cold temperature, KCl or LiCl stress. However, the overexpression of Th2CysPrx gene significantly increased the cell survival rate of yeast cells under oxidative, NaCl and NaHCO3 stress. In addition, the overexpression of Th2CysPrx gene also significantly improved the cell survival rate in recombinant yeast under stress caused by higher than 0.5 mol/L sorbitol, temperature above 44 ℃, or less than 0.007%(mass fraction) CdCl2. 【Conclusion】 Th2CysPrx gene can significantly enhance the tolerance of yeast cells not only to NaCl or NaHCO3 stress, but also to specific osmotic, high-temperature, oxidative, or heavy metal stresses, but exhibits no obvious tolerance to cold temperature, KCl or LiCl stress.

Key words

Tamarix hispida / yeast / peroxidoxin(Prxs) / abiotic stress

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WANG Yuanyuan , LIU Baichao , JIANG Bo , et al . Th2CysPrx gene enhanced abiotic stress tolerances of Saccharomyces cerevisiae[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2022, 46(4): 87-94 https://doi.org/10.12302/j.issn.1000-2006.202105023

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