Variations in the contents of leaf pigments and polyphenols and photosynthesis traits in Cyclocarya paliurus with different leaf colors

XU Zhanhong, ZHU Ying, JIN Huiying, SUN Caowen, FANG Shengzuo

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (2) : 103-110.

PDF(1969 KB)
南京林业大学学报（自然科学版）
PDF(1969 KB)
JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (2) : 103-110. DOI: 10.12302/j.issn.1000-2006.202105048

Variations in the contents of leaf pigments and polyphenols and photosynthesis traits in Cyclocarya paliurus with different leaf colors

Author information +
History +

Abstract

【Objective】Cyclocarya paliurus is a multi-functional tree species. To provide a theoretical basis for the breeding of C. paliurus, we examined differences in leaf pigments, flavonoid, and tannin concentrations and photosynthetic traits in two types of C. paliurus with different leaf colors.【Method】Based on seasonal dynamics of leaf growth, young and mature leaves of red-leafed C. paliurus and green-leafed C. paliurus were collected to determine anthocyanin, chlorophyll, carotenoid, tannin, and flavonoid concentration, and net photosynthetic rate (Pn) and PSII maximum photochemical efficiency (Fv/Fm) of the four leaf types. The collected data were analyzed and compared across different leaf types using one-way analysis of variance.【Result】 Anthocyanin concentrations were significantly higher in red fresh leaves (RF) than that in three other leaf types (P < 0.05). However, the content of chlorophyll and carotenoid was significantly lower in RF than those in red mature leaves (RM) and green mature leaves (GM) (P < 0.05); however, the differences between RF and green fresh leaves (GF) were not significant. There were no significant differences between RM and GM regarding flavonoid and tannin content at most sampling times (P > 0.05), whereas the tannin content was significantly higher in RM than in GM in autumn (P < 0.05). RF had a lower total chlorophyll content due to pronounced accumulation of anthocyanins, however, no significant difference in net photosynthetic rates (Pn) between RF and GF (P > 0.05) and between RM and GM were observed (P > 0.05). 【Conclusion】 High anthocyanin concentrations were the main reason for red coloration of RF. There were some differences regarding tannin and flavonoid concentrations between red and green leaves of C. paliurus, but generally no significant difference was observed between them. During the RF phase, the polyphenol content was higher, however, there was no significant difference between red-leaf C. paliurus and green-leaf C. paliurus during most of the mature leaf phase (P > 0.05). Therefore, the phenotypic characteristics of red leaves can only provide a reference for screening superior individuals of C. paliurus for medicinal use. However, in July and August, anthocyanin contents in C. paliurus leaves remained relatively high, and the leaves showed a red coloration, thus showing a higher and relatively stable ornamental value compared with green-leaved C. paliurus.

Key words

Cyclocarya paliurus / leaf color / anthocyanins / flavonoid / tannins / photosynthesis traits

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations
XU Zhanhong , ZHU Ying , JIN Huiying , et al . Variations in the contents of leaf pigments and polyphenols and photosynthesis traits in Cyclocarya paliurus with different leaf colors[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2022, 46(2): 103-110 https://doi.org/10.12302/j.issn.1000-2006.202105048

References

List( Publishing order | Descend order by publishing year | Descend order by cited within ) Chart analysis
[1]
TELLEZ P, ROJAS E, VAN BAEL S.Red coloration in young tropical leaves associated with reduced fungal pathogen damage[J]. Biotropica, 2016, 48(2):15053. DOI: 10.1111/btp.12303.
https://doi.org/10.1111/btp.12303
Cited in this article [1]
[2]
COONEY L J, LOGAN B A, WALSH M J L, et al. Photoprotection from anthocyanins and thermal energy dissipation in senescing red and green Sambucus canadensis peduncles[J]. Environ Exp Bot, 2018, 148:27-34. DOI: 10.1016/j.envexpbot.2017.12.019.
https://doi.org/10.1016/j.envexpbot.2017.12.019
https://linkinghub.elsevier.com/retrieve/pii/S0098847217303489
Cited in this article [1]
[3]
KURSAR T A, COLEY P D. Delayed greening in tropical leaves:an antiherbivore defense?[J]. Biotropica, 1992, 24(2):256. DOI: 10.2307/2388520.
https://doi.org/10.2307/2388520
https://www.jstor.org/stable/2388520?origin=crossref
Cited in this article [1]
[4]
HARVAUX M, KLOPPSTECH K. The protective functions of carotenoid and flavonoid pigments against excess visible radiation at chilling temperature investigated in Arabidopsis npq and tt mutants[J]. Planta, 2001, 213(6):953-966. DOI: 10.1007/s004250100572.
https://doi.org/10.1007/s004250100572
http://link.springer.com/10.1007/s004250100572
Cited in this article [1]
[5]
COLEY P D, AIDE T M. Red coloration of tropical young leaves:a possible antifungal defence?[J]. J Trop Ecol, 1989, 5(3):293-300. DOI: 10.1017/s0266467400003667.
https://doi.org/10.1017/s0266467400003667
https://www.cambridge.org/core/product/identifier/S0266467400003667/type/journal_article
Cited in this article [1]
[6]
孙明霞, 王宝增, 范海, 等. 叶片中的花色素苷及其对植物适应环境的意义[J]. 植物生理学通讯, 2003, 39(6):688-694.
SUN M X, WANG B Z, FAN H, et al. Anthocyanins of leaves and their enviromental significance in plant stress responses[J]. Plant Physiol Commun, 2003, 39(6):688-694. DOI: 10.13592/j.cnki.ppj.2003.06.051.
https://doi.org/10.13592/j.cnki.ppj.2003.06.051
Cited in this article [1]
[7]
GONG W C, LIU Y H, WANG C M, et al. Why are there so many plant species that transiently flush young leaves red in the tropics?[J]. Front Plant Sci, 2020, 11:83. DOI: 10.3389/fpls.2020.00083.
https://doi.org/10.3389/fpls.2020.00083
https://www.frontiersin.org/article/10.3389/fpls.2020.00083/full
Cited in this article [3]
[8]
HEDIN P A, JENKINS J N, PARROTT W L. Evaluation of flavonoids in Gossypium arboreum (L.) cottons as potential source of resistance to tobacco budworm[J]. J Chem Ecol, 1992, 18(2):10514. DOI: 10.1007/BF00993746.
https://doi.org/10.1007/BF00993746
Cited in this article [1]
[9]
武予清, 郭予元. 棉花单宁-黄酮类化合物对棉铃虫的抗性潜力[J]. 生态学报, 2001, 21(2):286-289.
WU Y Q, GUO Y Y. Potential resistance of tannins-flavoniods in upland cotton against Helicpverpa armigera (Hübner)[J]. Chin J Plant Ecol, 2001, 21(2):286-289. DOI: 10.3321/j.issn:1000-0933.2001.02.018.
https://doi.org/10.3321/j.issn:1000-0933.2001.02.018
Cited in this article [1]
[10]
张飞萍, 邓秀明, 陈清林, 等. 毛竹尖胸沫蝉危害对毛竹枝叶黄酮和单宁含量的影响[J]. 竹子研究汇刊, 2003, 22(1):43-46.
ZHANG F P, DENG X M, CHEN Q L, et al. Effects of Aphrophora natabitis’ damage on the contents of flavone and tannin in tips and leaves of Phyllostachys pubescens[J]. J Bamboo Res, 2003, 22(1):43-46. DOI: 10.3969/j.issn.1000-6567.2003.01.009.
https://doi.org/10.3969/j.issn.1000-6567.2003.01.009
Cited in this article [1]
[11]
王锋, 邓洁红, 谭兴和, 等. 花色苷及其共色作用研究进展[J]. 食品科学, 2008, 29(2):472-476.
WANG F, DENG J H, TAN X H, et al. Research progress on anthocyanins and copigmentation[J]. Food Sci, 2008, 29(2):472-476. DOI: 10.3321/j.issn:1002-6630.2008.02.104.
https://doi.org/10.3321/j.issn:1002-6630.2008.02.104
Cited in this article [1]
[12]
LIU Y, FANG S Z, YANG W X, et al. Light quality affects flavonoid production and related gene expression in Cyclocarya paliurus[J]. J Photochem Photobiol B, 2018, 179:66-73. DOI: 10.1016/j.jphotobiol.2018.01.002.
https://doi.org/10.1016/j.jphotobiol.2018.01.002
https://linkinghub.elsevier.com/retrieve/pii/S1011134417309569
Cited in this article [1]
[13]
ZHOU M M, LIN Y, FANG S Z, et al. Phytochemical content and antioxidant activity in aqueous extracts of Cyclocarya paliurus leaves collected from different populations[J]. PeerJ, 2019, 7:e6492. DOI: 10.7717/peerj.6492.
https://doi.org/10.7717/peerj.6492
https://peerj.com/articles/6492
Cited in this article [1]
[14]
方升佐, 洑香香. 青钱柳资源培育与开发利用的研究进展[J]. 南京林业大学学报(自然科学版), 2007, 31(1):95-100.
FANG S Z, FU X X. Progress and prospects on silviculture and utilization of Cyclocarya paliurus resources[J]. J Nanjing For Univ (Nat Sci Ed), 2007, 31(1):95-100. DOI: 10.3969/j.issn.1000-2006.2007.01.023.
https://doi.org/10.3969/j.issn.1000-2006.2007.01.023
Cited in this article [1]
[15]
林源, 陈培, 周明明, 等. 天然居群青钱柳叶主要生物活性物质及抗氧化活性研究[J]. 南京林业大学学报(自然科学版), 2020, 44(2):10-16,106.
LIN Y, CHEN P, ZHOU M M, et al. Key bioactive substances and their antioxidant activities in Cyclocarya paliurus (Batal.)Iljinskaja leaves collected from natural populations[J]. J Nanjing For Univ (Nat Sci Ed), 2020, 44(2):10-16,106. DOI: 10.3969/j.issn.1000-2006.201901045.
https://doi.org/10.3969/j.issn.1000-2006.201901045
Cited in this article [1]
[16]
凌岩, 秦健, 尚旭岚, 等. 施氮量对青钱柳幼苗生长和总酚积累的影响[J]. 植物资源与环境学报, 2020, 29(4):45-51.
LING Y, QIN J, SHANG X L, et al. Effect of applying amount of nitrogen on growth and total phenolic accumulation in Cyclocarya paliurus seedling[J]. J Plant Resour Environ, 2020, 29(4):45-51. DOI: 10.3969/j.issn.1674-7895.2020.04.06.
https://doi.org/10.3969/j.issn.1674-7895.2020.04.06
Cited in this article [1]
[17]
曹燕妮. 青钱柳叶次生代谢物质的指纹图谱建立及其地理变异研究[D]. 南京: 南京林业大学, 2018.
CAO Y N. Fingerprintestablishment of secondary metabolites in Cyclocarya paliurus leaves and their geographical variation patterns[D]. Nanjing: Nanjing Forestry University, 2018.
Cited in this article [1]
[18]
王全. 青钱柳种质资源次生代谢产物的差异及降血糖谱效关系初探[D]. 南京: 南京林业大学, 2019.
WANG Q. Study on the differences of secondary metabolites among germplasm resources and preliminary analysis on spectrum-effect relationship of hypoglycemic effect in Cyclocarya paliurus[D]. Nanjing: Nanjing Forestry University, 2019.
Cited in this article [1]
[19]
田力, 徐骋炜, 尚旭岚, 等. 青钱柳药用优良单株评价与选择[J]. 南京林业大学学报(自然科学版), 2021, 45(1):21-28.
TIAN L, XU C W, SHANG X L, et al. Evaluation and selection on superior individuals for medicinal use of Cyclocarya paliurus[J]. J Nanjing For Univ (Nat Sci Ed), 2021, 45(1):21-28.
Cited in this article [1]
[20]
唐前瑞, 陈德富, 陈友云, 等. 红檵木叶色变化的生理生化研究[J]. 林业科学, 2006, 42(2):111-115.
TANG Q R, CHEN D F, CHEN Y Y, et al. Changes of physiology and biochemistry during leaf color transformation in Loropetalum chinense var.rubrum[J]. Sci Silvae Sin, 2006, 42(2):111-115. DOI: 10.3321/j.issn:1001-7488.2006.02.019.
https://doi.org/10.3321/j.issn:1001-7488.2006.02.019
Cited in this article [1]
[21]
刘洋. 环境与基因型对青钱柳叶活性成分积累及其功效的影响[D]. 南京: 南京林业大学, 2019.
LIU Y. Effects of environment and genotype on accumulation and functions of bioactive constituents in Cyclocarya paliurus leaves[D]. Nanjing: Nanjing Forestry University, 2019.
Cited in this article [1]
[22]
PORRA R J, THOMPSON W A, KRIEDEMANN P E. Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents:verification of the concentration of chlorophyll standards by atomic absorption spectroscopy[J]. Biochim Biophys Acta (BBA) Bioenerg, 1989, 975(3):384-394. DOI: 10.1016/S00052728(89)80347-0.
https://doi.org/10.1016/S00052728(89)80347-0
https://linkinghub.elsevier.com/retrieve/pii/S0005272889803470
Cited in this article [1]
[23]
袁红娥, 严善春, 佟丽丽, 等. 剪叶损伤与昆虫取食对兴安落叶松(Larix gmelinii)针叶中缩合单宁诱导作用的差异[J]. 生态学报, 2009, 29(3):1415-1420.
YUAN H E, YAN S C, TONG L L, et al. Content differences of condensed tannin in needles of Larix gmelinii by cutting needles and insect feeding[J]. Chin J Plant Ecol, 2009, 29(3):1415-1420. DOI: 10.3321/j.issn:1000-0933.2009.03.039.
https://doi.org/10.3321/j.issn:1000-0933.2009.03.039
Cited in this article [1]
[24]
徐伟. 兴安落叶松诱导抗虫性研究[D]. 哈尔滨: 东北林业大学, 2006.
XU W. Study on the induced resistance of Larix gmelinii to insects[D]. Harbin: Northeast Forestry University, 2006.
Cited in this article [1]
[25]
岳喜良, 秦健, 洑香香, 等. 氮素水平对青钱柳叶片主要次生代谢物含量和抗氧化能力的影响[J]. 南京林业大学学报(自然科学版), 2020, 44(2):35-42.
YUE X L, QIN J, FU X X, et al. Effects of nitrogen fertilization on secondary metabolite accumulation andantioxidant capacity of Cycolcurya paliurus (Batal.)Iljinskaja leaves[J]. J Nanjing For Univ (Nat Sci Ed), 2020, 44(2):35-42. DOI: 10.3969/j.issn.10002006.201904048.
https://doi.org/10.3969/j.issn.10002006.201904048
Cited in this article [1]
[26]
袁明, 万兴智, 杜蕾, 等. 红花檵木叶色变化机理的初步研究[J]. 园艺学报, 2010, 37(6):949-956.
YUAN M, WAN X Z, DU L, et al. Mechanism on leaf color transformation of Loropetalum chinense olive var.rubrum[J]. Acta Hortic Sin, 2010, 37(6):949-956. DOI: 10.16420/j.issn.0513-353x.2010.06.014.
https://doi.org/10.16420/j.issn.0513-353x.2010.06.014
Cited in this article [1]
[27]
路买林, 陈梦娇, 张嘉嘉, 等. ‘红叶’杜仲叶色转变过程中叶片生理指标变化[J]. 南京林业大学学报(自然科学版), 2021, 45(1):86-92.
LU M L, CHEN M J, ZHANG J J, et al. Leaf physiological indicator changes in the transformation of leaves color of Eucommia ulmoides ‘Hongye’[J]. J Nanjing For Univ (Nat Sci Ed), 2021, 45(1):86-92. DOI: 10.12302/j.issn.10002006.202002034.
https://doi.org/10.12302/j.issn.10002006.202002034
Cited in this article [1]
[28]
DEAL D L, RAULSTON J C, HINESLEY L E. Leaf color retention,dark respiration,and growth of red-leafed Japanese maples under high night temperatures[J]. Jashs, 1990, 115(1):13540. DOI: 10.21273/jashs.115.1.135.
https://doi.org/10.21273/jashs.115.1.135
Cited in this article [1]
[29]
常仁杰. 高温胁迫下两种叶色四季秋海棠的生理生化响应研究[D]. 杭州: 浙江农林大学, 2013.
CHANG R J. Study on physio-logical and biochemical responses of two kinds of Begonia semperflorens in leaf color under high temperature stress[D]. Hangzhou: Zhejiang A & F University, 2013.
Cited in this article [1]
[30]
李颖畅, 齐凤元, 樊严. 提高花色苷稳定性的研究进展[J]. 中国调味品, 2009, 34(11):88-90,111.
LI Y C, QI F Y, FAN Y. Research advances of the stability of anthocyanins[J]. China Condiment, 2009, 34(11):88-90,111. DOI: 10.3969/j.issn.1000-9973.2009.11.019.
https://doi.org/10.3969/j.issn.1000-9973.2009.11.019
Cited in this article [1]
[31]
SHIKOV V, KAMMERER D R, MIHALEV K, et al. Colour stability of strawberry beverage fortified with polyphenolic copigments from rose (Rosa damascena Mill.) petals[J]. J Agr Food Chem, 2007, 8(3): 318-321. DOI: 10.1016/j.ifset.2007.03.004.
https://doi.org/10.1016/j.ifset.2007.03.004
https://pubs.acs.org/doi/abs/10.1021/jf60110a018
Cited in this article [1]
[32]
LUO H H, LI W J, ZHANG X, et al. In planta high levels of hydrolysable tannins inhibit peroxidase mediated anthocyanin degradation and maintain abaxially red leaves of Excoecaria Cochinchinensis[J]. BMC Plant Biol, 2019, 19(1):315. DOI: 10.1186/s12870-019903-y.
https://doi.org/10.1186/s12870-019903-y
https://doi.org/10.1186/s12870-019903-y
Cited in this article [1]
[33]
史宝胜, 卓丽环, 杨建民. 光照对紫叶李叶色发育的影响[J]. 东北林业大学学报, 2007, 35(4):168.
SHI B S, ZHUO L H, YANG J M. Effects of light on leaf color of redleaf cherry plum[J]. J Northeast For Univ, 2007, 35(4):168. DOI: 10.3969/j.issn.1000-5382.2007.04.006.
https://doi.org/10.3969/j.issn.1000-5382.2007.04.006
Cited in this article [1]
[34]
邓洁红, 位佳静, 刘永红, 等. 刺葡萄花色苷自聚合条件及水合动力学特性的研究[J]. 现代食品科技, 2015, 31(3):14450,164.
DENG J H, WEI J J, LIU Y H, et al. Self-association conditions and hydration kinetics of spine grape anthocyanins[J]. Mod Food Sci Technol, 2015, 31(3):14450,164. DOI: 10.13982/j.mfst.1673-9078.2015.3.025.
https://doi.org/10.13982/j.mfst.1673-9078.2015.3.025
Cited in this article [1]
[35]
DEMMIG-ADAMS B, ADAMS W W III. Photoprotection and other responses of plants to high light stress[J]. Annu Rev Plant Physiol Plant Mol Biol, 1992, 43:599-626. DOI: 10.1146/annurev.pp.43.060192.003123.
https://doi.org/10.1146/annurev.pp.43.060192.003123
https://www.annualreviews.org/toc/arplant.2/43/1
Cited in this article [1]
[36]
COLEY P D, KURSOR T A. Anti-herbivore defenses of young tropical leaves:physiological constraints and ecological trade-offs trop[J]. For Plant Ecophysiol, 1996:305-336. DOI: 10.1007/978-4613163-8_11.
https://doi.org/10.1007/978-4613163-8_11
Cited in this article [1]
[37]
LEE D W, BRAMMEIER S, SMITH A P. The selective advantages of anthocyanins in developing leaves of mango and cacao[J]. Biotropica, 1987, 19(1):40. DOI: 10.2307/2388458.
https://doi.org/10.2307/2388458
https://www.jstor.org/stable/2388458?origin=crossref
Cited in this article [1]
[38]
DOMINY N J, LUCAS P W, RAMSDEN L W, et al. Why are young leaves red?[J]. Oikos, 2002, 98(1):16376. DOI: 10.1034/j.1600-0706.2002.980117.x.
https://doi.org/10.1034/j.1600-0706.2002.980117.x.
Cited in this article [1]
[39]
李小康. 中红杨叶色变化的生理生化研究[D]. 郑州:河南农业大学, 2008.
LI X K. Study on changes of physiology and biochemistry during its leafcolor transformation of Populus×euramericana ‘Zhonghong’[D]. Zhengzhou: Henan Agricultural University, 2008.
Cited in this article [1]
[40]
庄猛. 不同季节红叶桃与紫叶李光合作用及相关生理特性的研究[D]. 南京: 南京农业大学, 2005.
ZHUANG M. Study on the photosynthesis and related physiological characters of red-leaf peach and purple-leaf plum in different seasons[D]. Nanjing: Nanjing Agricultural University, 2005.
Cited in this article [1]
[41]
刘弘, 李保印, 马杰, 等. 紫叶桃/绿叶桃光合特性及其影响因素研究[J]. 安徽农业科学, 2004, 32(2):318-320. DOI: 10.13989/j.cnki.0517-6611.2004.02.067.
https://doi.org/10.13989/j.cnki.0517-6611.2004.02.067
Cited in this article [1]
[42]
黄欣, 王华新, 孙开道, 等. 高温季节红花檵木叶片色素含量及净光合速率的变化[J]. 湖南农业科学, 2017(4):43-45.
HUANG X, WANG H X, SUN K D, et al. Changes of leaf pigment concentration and net photosynthetic rate of Loropetalum chinense var.rubrum in high temperature season[J]. Hunan Agric Sci, 2017(4):43-45. DOI: 10.16498/j.cnki.hnnykx.2017.004.012.
https://doi.org/10.16498/j.cnki.hnnykx.2017.004.012
Cited in this article [1]
[43]
陈晓林, 陈建华, 侯伯鑫, 等. 不同品种红花檵木光合特性的比较分析[J]. 中南林业科技大学学报, 2010, 30(5):117-122.
CHEN X L, CHEN J H, HOU B X, et al. Comparative analysis on photosynthetic characteristics of different varieties Loropetalum chinense var. rubrum[J]. J Central South Univ For Technol, 2010, 30(5):117-122. DOI: 10.14067/j.cnki.1673-923x.2010.05.033.
https://doi.org/10.14067/j.cnki.1673-923x.2010.05.033
Cited in this article [1]
PDF(1969 KB)

Accesses

Citation

Detail
Sections
Recommended
The full text is translated into English by AI, aiming to facilitate reading and comprehension. The core content is subject to the explanation in Chinese.

Author

Reader

Reviewer

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

/

〈 〉