Photosynthesis and hormone study of male and hermaphroditic Osmanthus fragrans at different flowering stages

doi:10.12302/j.issn.1000-2006.202107032

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (5): 75-80.doi: 10.12302/j.issn.1000-2006.202107032

Photosynthesis and hormone study of male and hermaphroditic Osmanthus fragrans at different flowering stages

ZHANG Cheng¹^,²(), WANG Xiaoyan¹^,²(), WANG Xianrong¹^,²^,^*(), DUAN Yifan¹^,²^,^*(), ZHANG Min¹^,², SHI Dawei¹, ZHU Yue¹^,², SONG Yanfeng¹^,², CHAI Zihan¹^,², LI Lan¹^,²

1. College of Biology and the Environment, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China
2. International Cultivar Registration Center for Osmanthus, Nanjing 210037, China

Received:2021-07-20 Revised:2021-11-28 Online:2022-09-30 Published:2022-10-19
Contact: WANG Xianrong,DUAN Yifan E-mail:chengz@njfu.edu.cn;wxyjessi@163.com;wangxianrong66@njfu.edu.cn;67181278@qq.com

Abstract

Abstract:

【Objective】Osmanthus fragrans with both male and hermaphroditic plants, possesses an extremely rare breeding system of androdioecy. The photosynthetic characteristics and endogenous hormone content of O. fragrans at different flowering stages were measured, and the changes of photosynthesis and endogenous hormone content in male and hermaphroditic O. fragrans at different flowering stages were compared, providing references for the evaluation of growth adaptability and subsequent physiological and ecological research of male and hermaphroditic O. fragrans.【Method】The photosynthetic characteristics of male and hermaphroditic flowers at different flowering stages were measured by Li-6400XT photosynthetic measurement system, and five plant hormones including auxin (IAA), jasmonic acid (JA), abscisic acid (ABA), gibberellin (GA₃) and zeatin nucleosides (ZRs) were measured by the enzyme-linked immunosorbent assay.【Result】The stomatal conductance and net photosynthetic rate of males were lower than that of hermaphroditic at different flowering stages. ABA had the highest hormone content among the five measured hormones. The content of JA continued to increase during the flowering period of male flowers and the content of ZRs continued to increase during the flowering period of hermaphroditic flowers.【Conclusion】Stomatal conductance was a key environmental factor affecting the net photosynthetic rate of O. fragrans, and was positively correlated with the net photosynthetic rate. The continuous accumulation of abscisic acid and jasmonic acid may lead to the mass abscission of male osmanthus petals. The accumulation of ZRs in the flowering stage of hermaphroditic flowers promoted the fruit development. The results provided basic physiological data for the different flowering stages of androdioecious O. fragrans, and provided references for the subsequent exploration of the mechanism of male flower petal abscission and fruit development.

Key words: Osmanthus fragrans, photosynthesis characteristics, plant hormone, androdioecy, flowering stage

CLC Number:

S718

ZHANG Cheng, WANG Xiaoyan, WANG Xianrong, DUAN Yifan, ZHANG Min, SHI Dawei, ZHU Yue, SONG Yanfeng, CHAI Zihan, LI Lan. Photosynthesis and hormone study of male and hermaphroditic Osmanthus fragrans at different flowering stages[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(5): 75-80.

Figures/Tables 4

Fig.1

Table 1

Table 2

Fig.2

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指标 index	花期 flowering stage	G_s	C_i	V_pdl
	初期	0.652^**	-0.369^**	-0.687^**
P_n (雄性male)	盛期	0.660^**	-0.089	0.140
	末期	0.591^**	-0.608^**	-0.322^**
	初期	0.963^**	0.579^**	-0.068
T_r (雄性male)	盛期	0.929^**	0.529^**	-0.014
	末期	0.689^**	0.387^**	0.548^**
	初期	0.485^**	0.185^*	-0.418^**
P_n (两性hermaphroditic)	盛期	0.511^**	-0.289^*	-0.624^**
	末期	0.782^**	0.007	-0.401^**
	初期	0.823^**	0.702^**	0.381^**
T_r (两性hermaphroditic)	盛期	0.955^**	0.749^**	-0.001
	末期	0.978^**	0.558^**	-0.192^*

性别 gender	花期 flowering stage	E_WUE/ (μmol · mmol^-1)	L_S /%	C_E/ (mmol· m^-2·s^-1)
雄性 male	初期	5.156	47.66	0.021
	盛期	4.800	47.28	0.019
	末期	4.327	42.22	0.020
两性 hermaphroditic	初期	7.446	52.90	0.036
	盛期	5.763	50.23	0.028
	末期	3.747	35.94	0.021

Photosynthesis and hormone study of male and hermaphroditic Osmanthus fragrans at different flowering stages

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