【Objective】Land-use change has an important impact on ecosystem service values. Affected by natural climate change and human activities, land-use in Jiangsu Province has undergone dramatic changes, and changes in the ecosystem structure have led to changes in ecosystem service values. By exploring the relationship between the long-term sequence of ecosystem service values and land-use changes in Jiangsu Province, this study provides a scientific basis for sustainable ecological development and land-use planning and management in Jiangsu Province.【Method】 Taking Jiangsu as the research areas, based on the land-use data of each city from 1980 to 2020, the land use transfer matrix was established to analyze the characteristics of land-use changes in Jiangsu Province. The unit area value equivalent method was used to calculate the ecosystem service values of the prefecture-level cities and regions in Jiangsu Province in different periods. Spatial interpolation analysis of ecosystem service values was conducted using GIS technology to explore the spatiotemporal dynamics of ecosystem service values in Jiangsu Province. The elastic coefficient was used to calculate the ratio of the rate of change in land use to the rate of change in ecosystem service values to explore the response of ecosystem service values to land-use changes. 【Result】The amount of cultivated land transferred out was the largest from 1980 to 2020, and the amount of construction land transferred in was the largest. The conversion of cultivated land to construction land is the most important way of land-use transfer in Jiangsu Province, and wetlands are mainly converted to cultivated land. The calculation results based on the unit area value method show that the total ecosystem service value of Jiangsu Province keeps increasing. During the study period, the hydrological regulation value was the largest and the water supply value was the smallest for different ecosystem service types. From the perspective of different cities, the total ecosystem service values of Suzhou, Yancheng and Huai'an were relatively high. The results of the spatial interpolation analysis showed that the total ecosystem service values of Jiangsu Province had obvious continuity and differences over a long time series, which was generally high in the east and low in the west, and high in the south and low in the north. The elastic coefficient results showed that regions with high elastic values were mainly located in the eastern coastal area, regions with medium elastic values were relatively widely distributed as flakes, and regions with low elastic values were relatively dispersed. 【Conclusion】The land-use change in Jiangsu Province was significant. The distribution of high value ecosystem services has an important relationship with the coverage of land-use types and ecosystem service value coefficients. Land-use changes affect the value of ecosystem services and simultaneously determine the elastic changes in ecosystem service values. The higher the ecosystem service value coefficient, the more sensitive the ecosystem service value is to land-use changes.