Effects of external 24-epibrassinolide (EBR) application on the development and physiological characteristics of Ginkgo biloba leaves

doi:10.12302/j.issn.1000-2006.202109026

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2023, Vol. 47 ›› Issue (4): 81-87.doi: 10.12302/j.issn.1000-2006.202109026

Special Issue: 乡村振兴视域下经济林果培育专题（Ⅱ）

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Effects of external 24-epibrassinolide (EBR) application on the development and physiological characteristics of Ginkgo biloba leaves

WANG Mengke(), YANG Xiaoming(), WANG Guibin, ZHOU Tinging, GUO Ying, GUO Jing

Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China

Received:2021-09-12 Revised:2022-02-24 Online:2023-07-30 Published:2023-07-20

Abstract

Abstract:

【Objective】 To better understand how 24-epibrassinolide (EBR) affects the development and physiological characteristics of ginkgo, different concentrations were sprayed onto ginkgo leaves, laying a theoretical basis upon which the oriented cultivation of ginkgo can be applied. 【Method】 Ginkgo leaves were placed in a randomized block design and treated with different concentrations of EBR (0-2.0 mg/L). The growth index, photosynthetic characteristics, and hormone content of EBR-treated annual seedlings were measured. 【Result】 The growth of ginkgo seedlings differed significantly following EBR treatment (P <0.05), with 1.0 mg/L EBR leading to increases of 23.6%, 52.6%, 140.2%, 87.7% and 69.1% in the maximum leaf length, leaf area, plant height, leaf weight and total weight, respectively, as compared to the control. Treatment with 1.5 mg/L (BR3) and 2.0 mg/L (BR4) led to increases of 40.2% and 41.5%, respectively, in the net photosynthetic rate as compared to the control. The maximum content of the endogenous hormones dihydrozein, indolepropionic acid, gibberellin, and abscisic acid and the minimum auxin content was observed under 1.5 mg/L EBR. The content of endogenous brassinolide was significantly positively correlated with the contents of methyl jasmonic and indolepropionic acid and negatively correlated with zeatin (R > 0.5, P < 0.05). 【Conclusion】 The results indicate that the exogenous spraying of ginkgo with EBR is an effective agronomic measure that could improve the net photosynthetic rate of gingko, while also regulating the content of endogenous hormones in the plant, thus promoting stem elongation, leaf area expansion, and leaf dry weight accumulation.

Key words: ginkgo(Ginkgo biloba), 24-epibrassinolide(EBR), endogenous hormone

CLC Number:

S156.4

WANG Mengke, YANG Xiaoming, WANG Guibin, ZHOU Tinging, GUO Ying, GUO Jing. Effects of external 24-epibrassinolide (EBR) application on the development and physiological characteristics of Ginkgo biloba leaves[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(4): 81-87.

Figures/Tables 6

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处理 treatment	苗高/cm seedling height	地径/cm ground diameter	叶长/cm leaf length	叶宽/cm leaf width	叶面积/cm² leaf area	叶厚/mm leaf thickness	根冠比 root to shoot ratio
CK	5.64±0.29 c	3.90±0.17 a	6.35±0.37 b	5.07±0.28 a	11.73±0.94 b	0.38±0.30 a	0.21±0.03 a
EBR1	9.54±0.35 b	3.99±0.16 a	7.15±0.08 ab	5.39±0.04 a	13.68±0.36 b	0.40±0.01 a	0.17±0.02 a
EBR2	13.55±0.60 a	4.24±0.11 a	7.85±0.77 a	5.86±0.57 a	17.90±1.74 a	0.42±0.00 a	0.15±0.01 a
EBR3	8.90±0.42 b	3.82±0.11 a	7.45±0.13 ab	6.01±0.21 a	15.35±1.05 ab	0.41±0.02 a	0.17±0.01 a
EBR4	9.80±0.53 b	4.13±0.17 a	6.84±0.25 ab	5.74±0.09 a	14.16±1.01 b	0.39±0.00 a	0.19±0.02 a

处理 treatment	叶 leaf	茎 stem	根 root	合计 total
CK	2.27±0.00 e	0.57±0.06 a	0.59±0.08 a	3.43±0.1 d
EBR1	2.61±0.00 d	0.70±0.08 a	0.57±0.09 a	3.88±0.16 c
EBR2	4.26±0.00 a	0.77±0.07 a	0.78±0.08 a	5.80±0.14 a
EBR3	2.92±0.00 c	0.71±0.05 a	0.62±0.04 a	4.25±0.07 c
EBR4	3.24±0.00 b	0.80±0.11 a	0.79±0.10 a	4.84±0.20 b

处理 treatment	胞间CO₂浓度/ (μmol·mol^-1) Ci	净光合速率/ (μmol·m^-2·s^-1) Pn	气孔导度/ (mmol·m^-2·s^-1) Gs	蒸腾速率/ (mmol·m^-2·s^-1) Tr	饱和水汽压差/ kPa VPD	水分利用效率/ (μmol·mol^-1) WUE
CK	420.33±10.27 a	3.73±0.29 b	36.33±1.20 c	1.10±0.06 b	3.07±0.03 a	3.33±0.32 a
EBR1	437.17±20.70 a	4.30±0.46 ab	49.00±4.68 bc	1.43±0.12 ab	2.98±0.07 a	3.07±0.95 a
EBR2	415.83±16.58 a	3.63±0.33 b	45.33±4.10 c	1.33±0.11 b	2.98±0.06 a	2.78±0.75 a
EBR3	439.40±5.54 a	5.42±0.40 a	65.40±6.59 ab	1.84±0.13 a	2.86±0.07 a	2.98±0.24 a
EBR4	468.25±4.59 a	5.50±0.44 a	78.50±8.02 a	1.88±0.28 a	2.43±0.14 b	3.03±0.50 a

处理 treatment	含量/(mg·g^-1) content				叶绿素a/b chlorophyll a/b	类胡萝卜素/ 总叶绿素 carotenoids/ chlorophyll
处理 treatment	叶绿素a chlorophyll	叶绿素b chlorophyll b	总叶绿素 chlorophyll	类胡萝卜素 carotenoids	叶绿素a/b chlorophyll a/b	类胡萝卜素/ 总叶绿素 carotenoids/ chlorophyll
CK	1.49±0.01 a	0.58±0.02 a	2.07±0.03 a	0.27±0.00 a	2.56±0.07 b	0.11±0.00 a
EBR1	1.48±0.04 a	0.59±0.09 a	2.06±0.13 a	0.27±0.02 a	2.60±0.3 b	0.11±0.00 a
EBR2	1.25±0.03 b	0.37±0.01 b	1.62±0.04 b	0.26±0.00 a	3.40±0.03 a	0.08±0.00 b
EBR3	1.47±0.01 a	0.51±0.01 a	1.98±0.02 a	0.28±0.01 a	2.90±0.06 ab	0.10±0.00 a
EBR4	1.50±0.01 a	0.59±0.05 a	2.08±0.06 a	0.27±0.01 a	2.57±0.18 b	0.11±0.00 a

处理 treatment	赤霉素 GA₃	脱落酸 ABA	玉米素 ZR	茉莉酸 JAMe	生长素 IAA	油菜素内酯 BR	双氢玉米素 DHZR	吲哚丙酸 IPA	赤霉素 GA₄
CK	2.91±0.11 c	74.48±1.14 b	6.75±0.33 b	24.92±0.84 a	48.94±2.05 a	7.77±0.30 a	2.75±0.14 b	8.73±0.21 b	5.49±0.24 b
EBR1	3.57±0.16 b	93.83±3.05 a	9.05±0.43 a	20.39±0.80 b	50.06±1.92 a	7.05±0.16 b	2.95±0.14 b	8.56±0.48 b	6.03±0.30 b
EBR2	3.51±0.05 b	67.43±2.80 b	7.86±0.39 ab	16.83±0.45 c	50.13±1.20 a	6.78±0.19 b	2.51±0.12 b	8.14±0.13 b	5.91±0.21 b
EBR3	3.96±0.11 a	94.29±2.39 a	6.76±0.37 b	22.93±1.05 a	35.51±1.43 b	7.77±0.02 a	4.10±0.15 a	10.64±0.34 a	8.39±0.31 a
EBR4	2.96±0.09 c	72.65±3.22 b	8.28±0.29 a	16.00±0.61 c	31.69±1.45 b	7.18±0.24 ab	2.82±0.12 b	9.21±0.39 b	6.03±0.23 b

Effects of external 24-epibrassinolide (EBR) application on the development and physiological characteristics of Ginkgo biloba leaves

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激素种类 hormones	GA₃	ABA	ZR	JAMe	IAA	BR	DHZR	IPA
ABA	0.549^*
ZR	0.029	0.077
JAMe	0.135	0.429	-0.575^*
IAA	0.002	-0.124	0.123	0.230
BR	0.008	0.325	-0.749^**	0.739^**	-0.212
DHZR	0.616^*	0.693^**	-0.320	0.437	-0.478	0.478
IPA	0.387	0.474	-0.417	0.315	-0.697^**	0.533^*	0.820^**
GA₄	0.705^**	0.559^*	-0.345	0.199	-0.480	0.380	0.779^**	0.713^**