Comparison of transcriptomic activity of Ilex integra and I. purpurea roots with NaCl treatments

ZHANG Qiang; ZHOU Peng; LIU Changlai; YU Yongfan; ZHANG Min; YANG Jiading

doi:10.12302/j.issn.1000-2006.202109054

Comparison of transcriptomic activity of Ilex integra and I. purpurea roots with NaCl treatments

ZHANG Qiang, ZHOU Peng, LIU Changlai, YU Yongfan, ZHANG Min, YANG Jiading

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (3) : 99-108.

PDF(3582 KB)

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (3) : 99-108. DOI: 10.12302/j.issn.1000-2006.202109054

Comparison of transcriptomic activity of Ilex integra and I. purpurea roots with NaCl treatments

ZHANG Qiang ¹ ,
ZHOU Peng ,
LIU Changlai ¹ ,
YU Yongfan ¹ ,
ZHANG Min ²^,^* ,
YANG Jiading ¹^,^*

Author information +

History +

Abstract

【Objective】 This research aims to investigate the difference in transcriptomic activity of Ilex integra and I. purpurea roots using NaCl salt treatments and provide insights for future study on the physiological mechanisms responsible for differential salt tolerance between these Ilex species. 【Method】 Two-year-old cutting seedlings of I. integra and I. purpurea were irrigated with half-strength Hoagland solution containing 250 mmol/L NaCl for salt treatments. Root samples were collected at 0(before salt treatment), 6 and 72 h after salt treatments. The total RNAs were extracted for transcriptomic RNA-Seq. Transcripts were assembled and differentially expressed genes (DEGs) were selected. The enriched KEGG functional pathways for the two species were compared, and the expression of genes involved in certain metabolic pathways was analyzed. 【Result】 There were 2 616 and 1 802 DEGs in 6 h-salt-treated and 72 h-salt-treated I. integra roots respectively, while there were 1 831 and 3 490 DEGs in 6 h-salt-treated and 72 h-salt-treated I. purpurea roots, respectively. The common enriched KEGG functional pathways between I. integra roots with 6 and 72 h salt treatments were plant hormone signal transduction, linoleic acid metabolism, carotenoid biosynthesis and betalain biosynthesis. The common enriched pathways between I. integra roots with 6 and 72 h salt treatments were plant hormone signal transduction, phenylpropanoid biosynthesis and carotenoid biosynthesis. Betalain biosynthesis and linoleic acid/arachidonic acid metabolism are unique pathways in I. integra roots during salt treatments. There were more up-regulated putative catalase genes with much higher expression levels in salt-treated I. integra roots than that in the I. purpurea roots. 【Conclusion】 The higher salt tolerance of I. integra roots may be associated with physiological processes such as plant hormone signal transduction, carotenoid biosynthesis, betalain biosynthesis and fatty acid metabolism. The higher expression of catalase genes during salt treatments may also contribute to the higher salt tolerance of I. integra roots.

Key words

Ilex integra / I. purpurea / roots / NaCl salt treatment / transcriptomic analysis / metabolic pathway

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

ZHANG Qiang , ZHOU Peng , LIU Changlai , et al . Comparison of transcriptomic activity of Ilex integra and I. purpurea roots with NaCl treatments[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2022, 46(3): 99-108 https://doi.org/10.12302/j.issn.1000-2006.202109054

References

List( Publishing order | Descend order by publishing year | Descend order by cited within ) Chart analysis

[1]	ZHAO C Z, ZHANG H, SONG C P, et al. Mechanisms of plant responses and adaptation to soil salinity[J]. Innov, 2020, 1(1):100017.DOI: 10.1016/j.xinn.2020.100017. https://doi.org/10.1016/j.xinn.2020.100017 Cited in this article [1]

[2]	赵宣, 韩霁昌, 王欢元, 等. 盐渍土改良技术研究进展[J]. 中国农学通报, 2016, 32(8):113-116. ZHAO X, HAN J C, WANG H Y, et al. Research progress of saline soil improvement technology[J]. Chin Agric Sci Bull, 2016, 32(8):113-116. Cited in this article [1]

[3]

阿吉艾克拜尔, 邵孝侯, 常婷婷, 等. 我国盐碱地改良技术和方法综述[J]. 安徽农业科学, 2013, 41(16):7269-7271.

HAJIAKBAR, SHAO

X H

, CHANG

T T

, et al. A review on improvement technology and methods of saline-alkali soil in China[J]. J Anhui Agric Sci, 2013, 41(16):7269-7271. DOI: 10.13989/j.cnki.0517-6611.2013.16.077.

https://doi.org/10.13989/j.cnki.0517-6611.2013.16.077

Cited in this article [1]

[4]

杨真, 王宝山. 中国盐渍土资源现状及改良利用对策[J]. 山东农业科学, 2015, 47(4):125-130.

YANG

, WANG

B S

. Present status of saline soil resources and countermeasures for improvement and utilization in China[J]. Shandong Agric Sci, 2015, 47(4):125-130.DOI: 10.14083/j.issn.1001-4942.2015.04.032.

https://doi.org/10.14083/j.issn.1001-4942.2015.04.032

Cited in this article [1]

[5]	万欣, 江浩, 王磊, 等. 江苏沿海滩涂土壤改良技术研究进展[J]. 江苏林业科技, 2017, 44(5):43-47. WAN X, JIANG H, WANG L, et al. Progress of soil amelioration technology in coastal beach in Jiangsu Province[J]. Jiangsu For Sci Technol, 2017, 44(5):43-47. Cited in this article [1]

[6]	王健, 李傲瑞. 我国盐碱地改良技术综述[J]. 现代农业科技, 2019(21):182-183,185. WANG J, LI A R. A summary of improvement techniques of saline-alkali land[J]. Mod Agric Sci Technol, 2019,(21):182-183,185. Cited in this article [1]

[7]

沈徐悦, 金荷仙, 陈蓉蓉, 等. NaCl胁迫对3种木兰科植物幼苗叶片部分生理指标的影响[J]. 植物资源与环境学报, 2020, 29(4):75-77.

SHEN

X Y

, JIN

H X

, CHEN

R R

, et al. Effect of NaCl stress on some physiological indexes of leaves of seedlings of three species in Magnoliaceae[M]. J Plant Resour Environ, 2020, 29(4):75-77. DOI: 10.3969 /j.issn.1674-7895.2020.04.11.

Cited in this article [1]

[8]

史成伟, 郭江艳, 翁行良, 等. 红果冬青种子繁殖与培育技术[J]. 绿色科技, 2019(21):116-118.

SHI

C W

, GUO

J Y

, WENG

X L

, et al. Seed propagation and cultivation techniques of Ilex Chinensis[J]. J Green Sci Technol, 2019(21):116-118.DOI: 10.16663/j.cnki.lskj.2019.21.046.

https://doi.org/10.16663/j.cnki.lskj.2019.21.046

Cited in this article [1]

[9]

徐斌芬, 王国明, 王美琴, 等. 全缘冬青和钝齿冬青的分布与繁殖技术[J]. 中国野生植物资源, 2007, 26(4):63-65.

B F

, WANG

G M

, WANG

M Q

, et al. Distribution and propagation of Ilex integra Thunb.and Ilex crenata Thunb.[J]. Chin Wild Plant Resour, 2007, 26(4):63-65.DOI: 10.3969/j.issn.1006-9690.2007.04.018.

https://doi.org/10.3969/j.issn.1006-9690.2007.04.018

Cited in this article [1]

[10]	YU Y F, ZHANG M, FENG J Y, et al. Physiological analysis reveals relatively higher salt tolerance in roots of Ilex integra than in those of Ilex purpurea[J]. J For Res, 2021, 9: 1-10.DOI: 10.1007/s11676-021-01386-w. https://doi.org/10.1007/s11676-021-01386-w Cited in this article [3]

[11]	GRABHERR M G, HAAS B J, YASSOUR M, et al. Full-length transcriptome assembly from RNA-seq data without a reference genome[J]. Nat Biotechnol, 2011, 29(7): 644-652. DOI: 10.1038/nbt.1883. https://doi.org/10.1038/nbt.1883 https://doi.org/10.1038/nbt.1883 Cited in this article [1]

[12]	WANG Z R, CUI Y Y, VAINSTEIN A, et al. Regulation of fig (Ficus carica L.) fruit color:metabolomic and transcriptomic analyses of the flavonoid biosynthetic pathway[J]. Front Plant Sci, 2017, 8:1990.DOI: 10.3389/fpls.2017.01990. https://doi.org/10.3389/fpls.2017.01990 Cited in this article [1]

[13]	ROY S J, NEGRÃO S, TESTER M. Salt resistant crop plants[J]. Curr Opin Biotechnol, 2014, 26:115-124.DOI: 10.1016/j.copbio.2013.12.004. https://doi.org/10.1016/j.copbio.2013.12.004 https://linkinghub.elsevier.com/retrieve/pii/S0958166913007192 Cited in this article [1]

[14]

ANAMIKA

, VERMA

, JERE

, et al. Transcriptomic profiling using next generation sequencing-advances,advantages,and challenges[M]//Next Generation Sequencing-Advances,Applications and Challenges. Rijeka, Croatia:InTech, 2016. DOI: 10.5772/61789.

https://doi.org/10.5772/61789

Cited in this article [1]

[15]	YU Z P, DUAN X B, LUO L, et al. How plant hormones mediate salt stress responses[J]. Trends Plant Sci, 2020, 25(11):1117-1130.DOI: 10.1016/j.tplants.2020.06.008. https://doi.org/10.1016/j.tplants.2020.06.008 https://linkinghub.elsevier.com/retrieve/pii/S1360138520302041 Cited in this article [1]

[16]	FRANK H A, COGDELL R J. Carotenoids in photosynthesis[J]. Photochem Photobiol, 1996, 63(3):257-264.DOI: 10.1111/j.1751-1097.1996.tb03022.x. https://doi.org/10.1111/j.1751-1097.1996.tb03022.x. http://www.blackwell-synergy.com/toc/php/63/3 Cited in this article [1]

[17]	HAN R M, ZHANG J P, SKIBSTED L H. Reaction dynamics of flavonoids and carotenoids as antioxidants[J]. Molecules, 2012, 17(2): 2140-2160.DOI: 10.3390/molecules17022140. https://doi.org/10.3390/molecules17022140 http://www.mdpi.com/1420-3049/17/2/2140 Cited in this article [1]

[18]	CAZZONELLI C I. Carotenoids in nature:Insights from plants and beyond[J]. Funct Plant Biol, 2011, 38(11):833-847.DOI: 10.1071/FP11192. https://doi.org/10.1071/FP11192 http://www.publish.csiro.au/?paper=FP11192 Cited in this article [1]

[19]

CHEN

X Y

, HAN

H P

, JIANG

, et al. Transformation of β-lycopene cyclase genes from Salicornia europaea and Arabidopsis conferred salt tolerance in Arabidopsis and tobacco[J]. Plant Cell Physiol, 2011, 52(5):909-921.DOI: 10.1093/pcp/pcr043.

https://doi.org/10.1093/pcp/pcr043

https://academic.oup.com/pcp/article-lookup/doi/10.1093/pcp/pcr043

Cited in this article [1]

[20]

TIAN

, DELLAPENNA

, ZEEVAART

J A D

. Effect of hydroxylated carotenoid deficiency on ABA accumulation in Arabidopsis[J]. Physiol Plant, 2004, 122(3):314-320.DOI: 10.1111/j.1399-3054.2004.00409.x.

https://doi.org/10.1111/j.1399-3054.2004.00409.x.

http://www.blackwell-synergy.com/toc/ppl/122/3

Cited in this article [1]

[21]	ZHAO S S, ZHANG Q K, LIU MY, et al. Regulation of plant responses to salt stress[J]. Int J Mol Sci, 2021, 22(9): 4609.DOI: 10.3390/ijms22094609. https://doi.org/10.3390/ijms22094609 https://www.mdpi.com/1422-0067/22/9/4609 Cited in this article [1]

[22]

, WU

, CHANG

, et al. Carotenoid deficiency impairs ABA and IAA biosynthesis and differentially affects drought and cold tolerance in rice[J]. Plant Mol Biol, 2013, 83(4/5):475-488.DOI: 10.1007/s11103-013-0103-7.

https://doi.org/10.1007/s11103-013-0103-7

http://link.springer.com/10.1007/s11103-013-0103-7

Cited in this article [1]

[23]	于思礼, 刘雪, 张昭宇, 等. 甜菜素的生物合成及其代谢调控进展[J]. 中国生物工程杂志, 2018, 38(8):84-91. YU S L, LIU X, ZHANG Z Y, et al. Advances of betalains biosynthesis and metabolic regulation[J]. China Biotechnol, 2018, 38(8):84-91.DOI: 10.13523/j.cb.20180811. https://doi.org/10.13523/j.cb.20180811 Cited in this article [1]

[24]

王长泉, 赵吉强, 陈敏, 等. 过氧化氢参与了黑暗诱导的盐地碱蓬叶片甜菜红素积累[J]. 植物生态学报, 2007, 31(4):748-752.

https://doi.org/10.17521/cjpe.2007.0095

Abstract

该文比较研究了黑暗和光照条件下C3盐生植物盐地碱蓬(Suaeda salsa)叶片甜菜红素积累和H2O2含量及其抗氧化酶活性的关系,实验分析了甜菜红素体外抗氧化性能,以期揭示诱导盐地碱蓬甜菜红素积累的可能机制以及甜菜红素积累的生理生态意义。结果表明：暗期处理和营养液中加入一定浓度的H2O2都明显促进盐地碱蓬叶片H2O2含量、甜菜红素的含量、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性,而且叶片中H2O2含量与甜菜红含量、SOD和CAT活性具有正相关性；盐地碱蓬甜菜红素体外清除羟自由基的能力明显强于维生素C,而清除超氧阴离子能力低于维生素C。这些结果表明：黑暗作为一种环境胁迫因子诱导盐地碱蓬叶片甜菜红素的积累可能是由自由基介导的,甜菜红素的积累可能与提高植物的抗氧化能力有关。

WANG

C Q

, ZHAO

J Q

, CHEN

, et al. Involvement of hydrogen peroxide in betacyanin accumulation induced by dark in leaves of Suaeda salsa[J]. Chin J Plant Ecol, 2007, 31(4):748-752.

https://doi.org/10.17521/cjpe.2007.0095

http://www.plant-ecology.com/EN/10.17521/cjpe.2007.0095

Cited in this article [1]

[25]

王佺珍, 刘倩, 高娅妮, 等. 植物对盐碱胁迫的响应机制研究进展[J]. 生态学报, 2017, 37(16):5565-5577.

WANG

Q Z

, LIU

, GAO

Y N

, et al. Review on the mechanisms of the response to salinity-alkalinity stress in plants[J]. Acta Ecol Sin, 2017, 37(16):5565-5577.DOI: 10.5846/stxb201605160941.

https://doi.org/10.5846/stxb201605160941

Cited in this article [1]

[26]	OKAZAKI Y, SAITO K. Roles of lipids as signaling molecules and mitigators during stress response in plants[J]. Plant J, 2014, 79(4):584-596.DOI: 10.1111/tpj.12556. https://doi.org/10.1111/tpj.12556 http://doi.wiley.com/10.1111/tpj.2014.79.issue-4 Cited in this article [1]

[27]	GUO Q, LIU L, BARKLA B J. Membrane lipid remodeling in response to salinity[J]. Int J Mol Sci, 2019, 20(17):4264.DOI: 10.3390/ijms20174264. https://doi.org/10.3390/ijms20174264 https://www.mdpi.com/1422-0067/20/17/4264 Cited in this article [1]

[28]

TSYDENDAMBAEV

V D

, IVANOVA

T V

, KHALILOVA

L A

, et al. Fatty acid composition of lipids in vegetative organs of the halophyte Suaeda altissima under different levels of salinity[J]. Russ J Plant Physiol, 2013, 60(5):661-671.DOI: 10.1134/s1021443713050142.

https://doi.org/10.1134/s1021443713050142

http://link.springer.com/10.1134/S1021443713050142

Cited in this article [1]

[29]

LIU

S S

, WANG

W Q

, LI

, et al. Antioxidants and unsaturated fatty acids are involved in salt tolerance in peanut[J]. Acta Physiol Plant, 2017, 39(9):1-10.DOI: 10.1007/s11738-017-2501-y.

https://doi.org/10.1007/s11738-017-2501-y

http://link.springer.com/10.1007/s11738-016-2300-x

Cited in this article [1]

[30]

SHANAB

S M M

, HAFEZ

R M

, FOUAD

A S

. A review on algae and plants as potential source of arachidonic acid[J]. J Adv Res, 2018, 11:3-13.DOI: 10.1016/j.jare.2018.03.004.

https://doi.org/10.1016/j.jare.2018.03.004

https://linkinghub.elsevier.com/retrieve/pii/S2090123218300389

Cited in this article [1]

[31]

SAVCHENKO

, WALLEY

J W

, CHEHAB

E W

, et al. Arachidonic acid: an evolutionarily conserved signaling molecule modulates plant stress signaling networks[J]. Plant Cell, 2010, 22(10):3193-3205.DOI: 10.1105/tpc.110.073858.

https://doi.org/10.1105/tpc.110.073858

https://academic.oup.com/plcell/article/22/10/3193/6094857

Cited in this article [1]

[32]

GONDIM

F A

, GOMES-FILHO

, COSTA

J H

, et al. Catalase plays a key role in salt stress acclimation induced by hydrogen peroxide pretreatment in maize[J]. Plant Physiol Biochem, 2012, 56:62-71.DOI: 10.1016/j.plaphy.2012.04.012.

https://doi.org/10.1016/j.plaphy.2012.04.012

https://linkinghub.elsevier.com/retrieve/pii/S0981942812000940

Cited in this article [1]

PDF(3582 KB)

Accesses

Citation

Detail

Sections

Recommended

The full text is translated into English by AI, aiming to facilitate reading and comprehension. The core content is subject to the explanation in Chinese.

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

〈

〉

Received	Accepted	Published
2021-09-27	2021-11-08	2022-05-30
Issue Date
2022-06-10

Please choose a citation manager

Content to export

Abstract

Key words

Cite this article

{{custom_sec.title}}

{{custom_sec.title}}

References

Author

Reader

Reviewer

模态框（Modal）标题

Please choose a citation manager

Content to export

Abstract

Key words

Cite this article

{{custom_sec.title}}

{{custom_sec.title}}

References

Author

Reader

Reviewer