Changes of coloration and pigment compositions during leaf development of Osmanthus fragrans colour group cultivar

CUI Qi, WU Yun, LI Dongze, WU Fan, HAN Ruilian, HUANG Junhua, HU Shaoqing

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2023, Vol. 47 ›› Issue (2) : 79-86.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2023, Vol. 47 ›› Issue (2) : 79-86. DOI: 10.12302/j.issn.1000-2006.202109057

Changes of coloration and pigment compositions during leaf development of Osmanthus fragrans colour group cultivar

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Abstract

【Objective】 This study aimed to explore the relationship between leaf color changes and pigment composition in Osmanthus fragrans color group during the leaf development. 【Method】 Different-colored leaves of the O. fragrans color group cultivar ‘Qiannan Guifei’ were selected from five developmental stages (S1-S5) for phenotype mensuration using the Royal Horticultural Society Color Chart and colorimeter. Chlorophyll and carotenoid concentrations of the leaves were determined using visible light spectrophotometry. Flavonoids were qualitatively and quantitatively characterized using ultra-performance liquid chromatography triple-time-of-flight/mass spectrometry (UPLC-Triple-TOF/MS). The correlation between leaf color and pigment components was also analyzed. 【Result】 The results indicated that the changes in leaf color could be roughly divided into two stages in relation to the leaf development: the first stage was from red to yellow (S1-S3), and the second stage was from yellow to green (S3-S5). The color parameters L* and b* initially increased and then decreased, while the values of a* and C* decreased. Eight flavonoids were detected in the leaves of ‘Qiannan Guifei’, including one anthocyanidin (Cy3R), three flavones (Ap7G, Ap7R and vicenin-2), and four flavonols (Km3R, Km7G, Km3G7R and Qu3R). The quantitative analysis showed that chlorophyll and carotenoid concentrations were relatively low and stable during stages S1-S3, whereas the concentrations and ratios of the two pigments increased significantly during stages S3-S5. Cy3R concentration significantly decreased from stages S1 to stage S3, and it occurred in undetectable trace amounts in stages S3-S5. The concentrations of Qu3R, Km3R, Km7G and Ap7G also decreased from stage S1 to stage S5, while vicenin-2 and Km3G7R showed a continuous increase. Ap7R was stable from stage S1 to stage S3, and then it initially decreased and then increased. 【Conclusion】 The decrease of anthocyanidin (Cy3R), flavone (Ap7G) and flavonols (Qu3R, Km3R and Km7G) was critical in promoting changes in leaf color from red to yellow in ‘Qiannan Guifei’, while the increase in the ratio of chlorophylls to carotenoids resulted in the leaves changing from yellow to green.

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Osmanthus fragrans colour group / leaf color / chlorophyll / flavonoid / anthocyanin / ‘Qiannan Guifei’

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CUI Qi , WU Yun , LI Dongze , et al . Changes of coloration and pigment compositions during leaf development of Osmanthus fragrans colour group cultivar[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2023, 47(2): 79-86 https://doi.org/10.12302/j.issn.1000-2006.202109057

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