Optimization of the tissue culture technology system of Dicranopteris pedata

ZHANG Ganglong, ZHU Enyong, LIU Liting, YANG Jiaqi, WANG Yannan, MO Xiaoyong

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2023, Vol. 47 ›› Issue (2) : 107-114.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2023, Vol. 47 ›› Issue (2) : 107-114. DOI: 10.12302/j.issn.1000-2006.202110011

Optimization of the tissue culture technology system of Dicranopteris pedata

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Abstract

【Objective】 Optimizing tissue culture techniques of Dicranopteris pedata is needed to improve the success rate and productivity of tissue culture.【Method】 Dicranopteris pedata spores were used as explants to study the relationship between the explant collection time, disinfection time and spore germination rate. By using the different concentration of inorganic salt and sucrose, along with the type and concentration of the plant growth regulator in the medium, the optimal medium formula for the spore germination, prothallus proliferation and sporophyte formation were studied.【Result】 March was the optimal time for collecting spores; the optimal treatment for inducing spore germination was DKW + NaClO (5%) disinfection for 15 s, with which the germination rate was 97.15%. Prothallus appeared after 20 days and the optimal medium for prothallus proliferation was 1/2 MS + sucrose 5 g/L + 6-BA 0.5 mg/L + KT 0.5 mg/L. The mass proliferation multiple was 7.28 after 30 days; the optimal medium for sporophyte induction was 1/2 MS + sucrose 30 g/L + NAA 1.0 mg/L; sporophytes appeared after 8 days, and the formation rate was 53.33%.【Conclusion】 Spore activity of Dicranopteris pedata is related to the growth area and formation month, and the activity of spores is strongest in Guangzhou in March. In the case of low bottle seedling rates of young sporophytes, the seedling breeding technology with efficient proliferation of prothallus green globular body as the core has been developed, which will contribute to the realization of large-scale and rapid production of Dicranopteris pedata seedlings.

Key words

Dicranopteris pedata / spore collection time / spore activity / prothallus green globular body (PGGB) / ecological restoration

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ZHANG Ganglong , ZHU Enyong , LIU Liting , et al . Optimization of the tissue culture technology system of Dicranopteris pedata[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2023, 47(2): 107-114 https://doi.org/10.12302/j.issn.1000-2006.202110011

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