Study on the physiological and biochemical changes of Phoebe sheareri seed during its dormancy breaking

LIU Miao, GAO Handong, GAO Yan, XUE Xiaoming

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2023, Vol. 47 ›› Issue (2) : 9-17.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2023, Vol. 47 ›› Issue (2) : 9-17. DOI: 10.12302/j.issn.1000-2006.202110038

Study on the physiological and biochemical changes of Phoebe sheareri seed during its dormancy breaking

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Abstract

【Objective】 In this study, experiments were carried out to explore the dynamic changes of nutrient contents, enzyme activities and endogenous hormones during the seed stratification of Phoebe sheareri. Additionally, this study was conducted to reveal the internal physiological mechanisms involved in seed dormancy and provide a theoretical basis for its dormancy release and germination. 【Method】 Fresh and full seeds of P. sheareri were treated with gibberellin and stratification was conducted; the physiological indicators during stratification were also determined. 【Result】 The seed dormancy of P. sheareri was relieved by stratification for 63 days. The germination rate of low temperature and variable temperature stratification was 85.83% and 72.75%, respectively. The initial starch content was 467.88 mg/g and decreased gradually during stratification. Soluble sugar, soluble protein and crude fat were decomposed to varying degrees. Under the two kinds of stratification treatments, the soluble sugar content in the seeds of P. sheareri changed greatly, from decreasing to increasing, and then decreasing again. The soluble protein content in P. sheareri seeds increased initially and then decreased under two stratification treatments. On the whole, the crude fat content in the seeds of P. sheareri showed a gradually decreasing trend under each stratification treatment. The activities of amylase, APA and POD were increased. This indicated that the two stratification treatments could promote the continuous activation of APA and POD activities. What’s more, endogenous hormones such as IAA, IPA, GA3 and ABA played an important role in the regulation of seed dormancy and germination. During the whole stratification process, the contents of IAA, IPA and GA3 increased, while the contents of ABA decreased, and the mass ratios of IAA/ABA, IPA/ABA and GA3/ABA increased. 【Conclusion】 There was medium dormancy in seeds of P. sheareri. Gibberellin and stratification could release dormancy, and cold stratification was better to accelerate seed germination. Starch was the main storage material in the seeds of P. sheareri. The transformation of nutrients and the coordination of endogenous hormones played an important role in the seeds dormancy release.

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Phoebe sheareris / seeds / germination / dormancy / physiological and biochemical indicators

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LIU Miao , GAO Handong , GAO Yan , et al. Study on the physiological and biochemical changes of Phoebe sheareri seed during its dormancy breaking[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2023, 47(2): 9-17 https://doi.org/10.12302/j.issn.1000-2006.202110038

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