Genetic diversity analyses of Phoebe bournei representative populations in Fujian Province based on SSR markers

FENG Yining, LI Yingang, QI Ming, ZHOU Pengyan, ZHOU Qi, DONG Le, XU Li’an

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (4) : 102-108.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (4) : 102-108. DOI: 10.12302/j.issn.1000-2006.202111016

Genetic diversity analyses of Phoebe bournei representative populations in Fujian Province based on SSR markers

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Abstract

【Objective】 Phoebe bournei is a precious timber tree, and listed as a national Class Ⅱ key-protected wild plant. This study aimed to understand the genetic variation levels and structural characteristics of the three representative and well-preserved natural populations (Luoboyan, Shunchang and Zhenghe) of P. bournei in Fujian Province, China, and explore the causes of genetic variation to provide a basis for the protection and utilization of natural populations. 【Method】 We used 18 self-developed polymorphic SSR markers to select 88 samples from the three populations. The effective number of alleles (Ne), observed heterozygosity (Ho), expected heterozygosity (He), and gene differentiation coefficient (Fst) were analyzed using Popgene 32 software. Besides, Structure software was used to study the genetic structure of the populations. 【Result】 The average expected heterozygosity of the three populations was 0.629, indicating the relatively rich genetic diversity of the three populations. In addition, the average observed heterozygosity of the three populations was significantly lower than expected, with a high inbreeding degree (F = 0.280). In particular, the Ho/He ratio of the Luobuyan population was large (0.399/0.608), with a high degree of inbreeding (0.378). The molecular variation analysis revealed that the variations in P. bournei populations were mainly from within, and a moderate degree of differentiation existed between populations (Fst = 0.197). The cluster analysis results indicated that the samples from the three populations could be divided into two groups, with an obvious genetic differentiation between them. Luoboyan and Shunchang populations comprised the first group because of their close geographical locations, and Zhenghe population formed the second because of being located distantly. 【Conclusion】 The three representative populations of P. bournei in Fujian Province had the high genetic diversity but small population characteristics, with a relatively high degree of inbreeding within the populations. However, geographical isolation and human activities had caused the P. bournei populations to attain a certain degree of genetic differentiations. Measures should be adopted to enable the full outcrossing of the populations to maintain the high genetic diversity of P. bournei.

Key words

Phoebe bournei / SSR markers / genetic diversity / genetic differentiation / genetic structure

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FENG Yining , LI Yingang , QI Ming , et al . Genetic diversity analyses of Phoebe bournei representative populations in Fujian Province based on SSR markers[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2022, 46(4): 102-108 https://doi.org/10.12302/j.issn.1000-2006.202111016

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