Effects of soil different preservation time on the soil animals separation results of Oribatida and Collembola by low temperature storage

doi:10.12302/j.issn.1000-2006.202112042

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2023, Vol. 47 ›› Issue (2): 213-218.doi: 10.12302/j.issn.1000-2006.202112042

Special Issue: 南京林业大学学报（自然科学版）2021-2023 森林保护学科论文引文专辑

Effects of soil different preservation time on the soil animals separation results of Oribatida and Collembola by low temperature storage

DU Zhiqi¹(), MENG Qingfan¹^,^*(), FENG Lichao²^,^*(), GE Jiarong¹, HUANG Jinbin¹, SHI Jingjing¹, LI Hongrui¹, CEN Zucai¹

1. College of Forestry, Beihua University, Jilin 132013, China
2. College of Agricultural, Jilin Agricultural Science and Technology University, Jilin 132101, China

Received:2021-12-30 Revised:2022-06-06 Online:2023-03-30 Published:2023-03-28

Abstract

Abstract:

【Objective】 This research aims to explore the effects of different storage time on the separation effects of soil animals under the condition of soil low temperature storage, so as to provide a reference for a large number of collection and separation of soil animals.【Method】 On October 6, 2018, the natural Mongolian oak (Quercus mongolica) forest in Songhua Lake Nature Reserve, Jilin City, the soil at different depths was drilled by the five point sampling method, brought back to the laboratory and stored in the indoor 4 ℃ refrigerator. The Berlese-Tullgren method was used to separate the soil animal samples of Oribatida and Collembola on the same day, 7, 14, 21 and 28 days later. The species and genera of Oribatida and Collembola were determined according to the morphological characteristics under the microscope.【Result】 With the increase of preservation time, the individual number and the number of species of soil animals of Oribatida and Collembola showed a downward trend; Under the same preservation time and different depths, the species of Oribatida and Collembola had significant differences with other depths (P<0.05), and showed a downward trend. The number of species of Oribatida and Collembolan collected in litter layer was significantly different from that in 5, 10 and 15 cm soil layers (P<0.05), and also showed a downward trend.【Conclusion】 Increasing the soil preservation time under cold storage will reduce the separation efficiency of medium-sized soil animals. The separation effect of Oribatida is the best on the day of soil collection, and the isolation of Collembola was not affected by soil storage time. Oribatida and Collembola animals were mainly collected in the litter layer. The soil depth increased while the soil animals decreased.

Key words: low temperature storage, forest soil, soil animal, preservation time, Oribatida, Collembola

CLC Number:

DU Zhiqi, MENG Qingfan, FENG Lichao, GE Jiarong, HUANG Jinbin, SHI Jingjing, LI Hongrui, CEN Zucai. Effects of soil different preservation time on the soil animals separation results of Oribatida and Collembola by low temperature storage[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(2): 213-218.

Figures/Tables 6

Table 1

Species and numbers of Oribatida under different storage times in Songhua Lake, Jilin Province"

学名 scientific name	不同时间物种数量/头 number under sorage time					总计/头 total	占比/% proportion
学名 scientific name	0 d	7 d	14 d	21 d	28 d	总计/头 total	占比/% proportion
Heminothrus peltifer	111	16	19	39	14	199	13.27
Nothrus palustris	94	8	2	12	2	118	7.87
镘藓甲螨Cepheus cepheiformis	33	13	26	22	3	97	6.47
Anderemaeus sp.	32	17	4	24	13	90	6.00
小矮汉甲螨Nanhermannia nana	78	1	0	2	2	83	5.53
球背甲螨Damaeidae sp.	18	23	26	11	2	80	5.33
Phthiracarus bryobius	31	11	9	10	10	71	4.73
短角翼甲螨Achipteria curta	27	21	5	10	3	66	4.40
淡红缝甲螨Hypochthonius rufulus	44	2	3	8	4	61	4.07
苞懒甲螨Nothrus borussicus	26	12	15	5	2	60	4.00
覆盖头甲螨Tectocepheus velatus	26	14	2	9	5	56	3.73
北丽甲螨Liacarus borealis	33	3	1	8	7	52	3.47
尼兰桂奥甲螨Lauroppia neerlandica	24	5	2	9	9	49	3.27
斜孔全大翼甲螨Pergalumna altera	20	16	3	3	2	44	2.93
Damaeus sp.	16	16	5	6	0	43	2.87
Belba compta	14	17	0	3	0	34	2.27
盾平懒甲螨Platynothrus peltifer	17	6	1	6	1	31	2.07
Pantelozetes sp.	14	6	2	7	1	30	2.00
圆上罗甲螨Epilohmannia ovata	5	7	9	6	1	28	1.87
日本礼服甲螨Trhypochthonius japonicus	16	0	2	6	4	28	1.87
全罗甲螨Perlohmannia sp.	14	2	4	2	0	22	1.47
懒甲螨Nothrus sp.	17	2	1	0	1	21	1.40
细珠甲螨Allodamaeus sp.	10	4	1	4	0	19	1.27
俄罗斯珠足甲螨Belba rossica	7	5	4	2	0	18	1.20
棒菌甲螨Scheloribates latipes	15	0	2	1	0	18	1.20
普通尖棱甲螨Ceratozetes mediocris	7	2	1	6	0	16	1.07
纹暗卷甲螨Atropacarus striculus	5	3	1	3	0	12	0.80
Hypochthoniella minutissima	8	1	0	1	1	11	0.73
单角菌板鳃甲螨Mycobates monocornis	7	0	1	2	1	11	0.73
Phthiracarus sp.	0	6	3	1	1	11	0.73
Euphthiracarus foveolatus	1	1	2	2	1	7	0.47
Nothrus anauniensis	2	1	0	2	2	7	0.47
粗毛巨袖螨Pergalumna intermedia	2	0	1	0	1	4	0.27
Lauritzenia major	1	0	0	0	0	1	0.07
长毛圆单翼甲螨Peloribates longisetosus	1	0	0	0	0	1	0.07
Oppiella sp.	0	1	0	0	0	1	0.07
合计sum	776	242	157	232	93	1 500	100.00

Table 1

Table 2

Table 3

Table 4

Table 5

Table 6

References 23

[1]	GILLER P S. The diversity of soil communities, the ‘Poor man’s tropical rainforest’[J]. Biodiversity & Conservation, 1996, 5(2): 135-168. DOI:10.1007/BF00055827.
[2]	BEST M L, WELSH J. The trophic role of a forest salamander: impacts on invertebrates, leaf litter retention, and the humification process[J]. Ecosphere, 2014, 5(2): 1-19. DOI:10.1890/ES13-00302.1.
[3]	VARGO D L. Soil invertebrates of American Samoa[J]. Micronesica, 2000, 33: 1-10.
[4]	贺纪正, 陆雅海, 傅伯杰. 土壤生物学前沿[M]. 北京: 科学出版社, 2015.
	HE J Z, LU Y H, FU B J. Frontier of soil biology[M]. Beijing: Science Press, 2015.
[5]	尹文英. 土壤动物学研究的回顾与展望[J]. 生物学通报, 2001, 36(8): 1-3.
	YIN W Y. Review and prospect of soil zoology research[J]. Bulletin of Biology, 2001, 36(8): 1-3. DOI:10.3969/j.issn.0006-3193.2001.08.001
[6]	黄红英, 李栋. 干漏斗实验装置的改进与创新[J]. 生物学通报, 2018, 53(1): 57-58.
	HUANG H Y, LI D. The improvement and innovation of Tullgren[J]. Bull Biol, 2018, 53(1): 57-58. DOI:10.3969/j.issn.0006-3193.2018.01.019.
[7]	IRMLER U. Changes in the fauna and its contribution to mass loss and N release during leaf litter decomposition in two deciduous forests[J]. Pedobiologia, 2000, 44(2): 105-118. DOI:10.1078/S0031-4056(04)70032-3.
[8]	殷秀琴, 马祝阳. Tullgren法对土壤动物的分离效率[J]. 东北师大学报(自然科学版), 2002, 34(2): 84-91.
	YIN X Q, MA Z Y. Segregate efficiency of Tullgren method to middle-small-sized soil animal[J]. J Northeast Norm Univ (Nat Sci Ed), 2002, 34(2): 84-91. DOI: 10.3321/j.issn:1000-1832.2002.02.017.
[9]	魏雪, 吴鹏飞. 土壤松紧度对Tullgren法分离小型节肢动物的影响[J]. 西南农业学报, 2014, 27(3): 1136-1142.
	WEI X, WU P F. Influence of soil compactness on extraction of soil microarthropods by tullgren method[J]. Southwest China J Agric Sci, 2014, 27(3):1136-1142. DOI:10.16213/j.cnki.scjas.2014.03.015.
[10]	铁烈华, 张林成, 冯茂松, 等. 烘虫温度和时间对中小型土壤动物烘虫分离效果的影响[J]. 四川农业大学学报, 2015, 33(1): 45-50.
	TIE L H, ZHANG L C, FENG M S, et al. Baking temperature and time on the effect of soil meso-and micro-fauna separation results[J]. J Sichuan Agric Univ, 2015, 33(1): 45-50. DOI:10.16036/j.issn.1000-2650.2015.01.008.
[11]	CARTER M, GREGORICH E. Soil sampling and methods of analysis[M].2nd Ed. Roca Rat: Lewis Publishing House, 2007.
[12]	ANDRIUZZI W S, ADAMS B J, BARRETT J E, et al. Observed trends of soil fauna in the Antarctic dry valleys: early signs of shifts predicted under climate change[J]. Ecology, 2018, 99(2):312-321.DOI:10.1002/ecy.2090.
[13]	尹文英. 中国亚热带土壤动物[M]. 北京: 科学出版社, 1992.
	YIN W Y. Subtropical soil animals of China[M]. Beijing: Science Press, 1992.
[14]	李春鸣. 土壤样品的采集和处理[J]. 西北民族大学学报(自然科学版), 2003, 24(3): 74-75.
	LI C M. Collection and treatment of soil samples[J]. J Northwest Minor Univ Natl (Nat Sci), 2003, 24(3): 74-75. DOI:10.14084/j.cnki.cn62-1188/n.2003.03.020.
[15]	吾玛尔·阿布力孜. 土壤螨类的采集与玻片标本的制作[J]. 生物学通报, 2012, 47(1): 57-59.
	Omar·Abliz. Collection of soil mites and preparation of slide specimens[J]. Microbiol China, 2012, 47(1): 57-59. DOI:10.3969/j.issn.0006-3193.2012.01.022.
[16]	尹文英. 中国土壤动物检索图鉴[M]. 北京: 科学出版社, 1998.
	YIN W Y. Pictorical keys to soil animals of China[M]. Beijing: Science Press, 1998.
[17]	青木淳一. 日本产土壤动物检索[M]. 东京: 东海大学出版会, 1993.
[18]	李东育, 陈晓露, 韩大勇, 等. 不同管理模式下苹果园昆虫群落物种组成及其多样性[J]. 江苏农业学报, 2021, 37(3):589-596.
	LI D Y, CHEN X L, HAN D Y, et al. Species composition and diversity of insect community in apple orchards with different management modes[J]. Jiangsu J Agr Sci, 2021, 37(3):589-596. DOI:10.3969/j.issn.1000-4440.2021.03.006.
[19]	勾影波, 苏永春. 土壤温度和含水量对螨类和弹尾类动物数量的影响[J]. 常熟理工学院学报, 2007(2): 57-62.
	GOU Y B, SU Y C. Effects of temperature and water content in soil on the quantity of Acarina and Collembola[J]. J Chang Inst Technol, 2007, 21(2): 57-62. DOI:10.16101/j.cnki.cn32-1749/z.2007.02.013.
[20]	邱军, 傅荣恕. 土壤温湿度对甲螨和跳虫数量的影响[J]. 山东师大学报(自然科学版), 2004, 19(4): 72-74.
	QIU J, FU R S. The effects of temperature and soil water content for the number of oribatida and Collembola[J]. J Shandong Norm Univ (Nat Sci), 2004, 19(4): 72-74. DOI:10.3969/j.issn.1001-4748.2004.04.021.
[21]	马辰. 长白山地森林土壤跳虫的分布格局及其对环境变化的响应[D]. 长春: 东北师范大学, 2019.
	MA C. The responses of soil Collembolan’s distribution pattern to environmental changes in the forests of Changbai Mountains[D]. Changchun: Northeast Normal University, 2019.
[22]	牙克甫·艾散江, 吾玛尔·阿布力孜, 买尔旦·艾斯卡尔, 等. 新疆7种微生境的土壤甲螨多样性特征[J]. 浙江农业学报, 2020, 32(4): 696-704.
	Yakup·Hasnjan, Omar·Abliz, Mardan·Askra, et al. Diversity characteristics of soil Oribatid mites in 7 microenvironments in Xinjiang[J]. Acta Agric Zhejiangensis, 2020, 32(4): 696-704. DOI:10.3969/j.issn.1004-1524.2020.04.17
[23]	NIELSEN U N, OSLER G H R, VAN DER WAL R, et al. Soil pore volume and the abundance of soil mites in two contrasting habitats[J]. Soil Biol Biochem, 2008, 40(6): 1538-1541. DOI:10.1016/j.soilbio.2007.12.029.

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时间/d time	个体数/头 number of individuals	物种数/种 number of species
0	77.60±85.31 a	17.50±6.02 a
7	24.40±27.77 b	9.70±7.02 b
14	31.40±46.76 b	7.40±2.63 b
21	23.20±14.74 b	9.60±4.27 b
28	10.00±5.83 b	5.90±3.38 b

学名 scientific name	不同时间物种数量/头 number under storage time					总计/头 total	占比/% proportion
学名 scientific name	0 d	7 d	14 d	21 d	28 d	总计/头 total	占比/% proportion
Paronychiurus japonica	163	44	38	94	50	389	44.56
Hymenaphorura maoerensis	25	4	4	39	31	103	11.80
Folsomia vtorovi	24	3	4	14	26	71	8.13
Protaphorura khanka	5	3	5	12	36	61	6.99
Tomocerus modificatus	8	2	2	28	5	45	5.15
Parisotoma hyonosenensis	24	3	0	12	3	42	4.81
Folsomia ancestor	10	8	1	5	8	32	3.67
Folsomia hidakana	8	8	3	3	0	22	2.52
Folsomia macrochaetosa	9	0	3	2	3	17	1.95
Folsomia palaearctica	3	2	1	4	0	10	1.15
Folsomia inoculata	5	0	4	0	1	10	1.15
铜色长跳Entomobrya aino	6	0	1	2	1	10	1.15
羽等跳Isotoma pinnata	2	0	0	6	1	9	1.03
Xenylla changchunnensis	0	0	1	4	4	9	1.03
Cyphoderus javanus	4	2	1	0	2	9	1.03
Folsomia ancestor	1	1	1	1	0	4	0.46
八眼符跳Folsomia octoculata	4	0	0	0	0	4	0.46
Protaphorura taimyrica	1	1	1	0	1	4	0.46
Koreanurina alba	1	1	1	0	1	4	0.46
Heteraphorura seolagensis	1	0	1	0	2	4	0.46
Folsomia fimetaria	4	0	0	0	0	4	0.46
Protaphorura genheensis	1	1	0	0	1	3	0.34
Ceratophysella succinea	3	0	0	0	0	3	0.34
Allonychiurus songi	2	0	0	0	0	2	0.23
Ceratophysella baichengensis	0	0	0	1	0	1	0.11
Desoria gracilliseta	1	0	0	0	0	1	0.11
合计sum	315	83	72	227	176	873	100.00

时间/d time	个体数/头 number of individuals	物种数/种 number of species
0	32.20±19.41 a	8.60±3.71 a
7	8.30±3.86 c	3.80±2.34 b
14	7.20±3.85 c	3.60±2.45 b
21	21.70±17.44 ab	6.30±3.49 ab
28	17.90±11.64 bc	6.30±3.56 ab

土层/cm soil level	甲螨类 Oribatida		跳虫类 Collembola
土层/cm soil level	个体数/头 number of individuals	物种数/种 number of species	个体数/头 number of individuals	物种数/种 number of species
枯落物层 litter layer	206.60±175.97 A	26.60±5.54 A	106.60±57.19 A	17.20±4.96 A
5	41.20±38.90 B	16.20±6.76 B	32.00±16.21 B	9.60±4.50 B
10	37.60±44.64 B	12.40±6.58 B	25.20±26.78 B	7.40±4.27 B
15	15.00±17.56 B	7.80±6.45 B	9.40±6.18 B	4.20±1.30 B

Effects of soil different preservation time on the soil animals separation results of Oribatida and Collembola by low temperature storage

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时间/d time	土层/cm soil level	跳虫类 Collembolan		甲螨类 Oribatida
时间/d time	土层/cm soil level	种数 species	数量/头 number	种数 species	数量/头 number
0	枯落物层	19	175	32	570
	5	14	31	23	82
	10	12	72	19	88
	15	4	19	15	36
7	枯落物层	12	56	26	193
	5	4	11	10	14
	10	5	12	6	18
	15	2	4	10	15
14	枯落物层	13	42	20	96
	5	6	20	16	35
	10	3	6	11	22
	15	3	4	4	6
21	枯落物层	14	147	28	184
	5	9	45	18	32
	10	5	14	10	13
	15	6	12	2	3
28	枯落物层	12	123	17	63
	5	10	31	7	15
	10	9	19	7	13
	15	4	3	2	2