Effects of Piriformospora indica on growth and drought resistance in Osmanthus fragrans under water deficit stress

MU Hongna, WANG Wei, FAN Lei, WU Chu, GUO Xiaohua, SUN Taoze

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2023, Vol. 47 ›› Issue (2) : 101-106.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2023, Vol. 47 ›› Issue (2) : 101-106. DOI: 10.12302/j.issn.1000-2006.202203014

Effects of Piriformospora indica on growth and drought resistance in Osmanthus fragrans under water deficit stress

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Abstract

【Objective】 The colonization effects of Piriformospora indica on Osmanthus fragrans under drought stress were analyzed. 【Method】 Two-year seedlings cultivated in pots with soil were used for this study. The experiment design was set up with the following parameters: inoculation (40 mL mycelium of P. indica) + drought (the soil water content is 20%-25% of the maximum field water capacity), inoculation + slight drought (45%-50% of the maximum field water capacity), inoculation + normal irrigation (65%-70% of the maximum field water capacity), control (40 mL inactivator) + drought, control + slight drought, and control + drought, six treatments in all. 【Result】 (1) Results showed that the colonization rate of P. indica was up to 81%. (2) Compared to the controls, the inoculated seedlings presented a better performance in plant height, stem diameter, and dry mass under both slight drought and drought stresses, especially the inoculation + drought groups, which showed significant or even extremely significant differences. (3) The protective enzyme activity of POD and SOD and the contents of proline and soluble sugar increased by 13.8%, 37.9%, 47.6% and 40.7%, respectively. (4) In addition, the MDA content decreased more quickly in the inoculation group than in the control group (control + drought). 【Conclusion】 This study found that the symbiosis of P. indica with O. fragrans played a positive role for O. fragrans seedlings in withstanding the negative effects of drought stress summer, most likely by stimulating the increase of SOD and POD enzyme activities and the significant accumulation of osmoregulatory substances. In contrast, inoculation with P. indica had no significant effect on the growth of O. fragrans under mild drought and normal water supply conditions.

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Piriformospora indica / Osmanthus fragrans / drought stress / osmotic adjustment / drought resistance

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MU Hongna , WANG Wei , FAN Lei , et al . Effects of Piriformospora indica on growth and drought resistance in Osmanthus fragrans under water deficit stress[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2023, 47(2): 101-106 https://doi.org/10.12302/j.issn.1000-2006.202203014

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