Effects of Piriformospora indica on growth and drought resistance in Osmanthus fragrans under water deficit stress

doi:10.12302/j.issn.1000-2006.202203014

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2023, Vol. 47 ›› Issue (2): 101-106.doi: 10.12302/j.issn.1000-2006.202203014

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Effects of Piriformospora indica on growth and drought resistance in Osmanthus fragrans under water deficit stress

MU Hongna¹^,²(), WANG Wei², FAN Lei², WU Chu², GUO Xiaohua², SUN Taoze¹^,²^,^*()

1. Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou 434025, China
2. College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China

Received:2022-03-04 Revised:2022-09-22 Online:2023-03-30 Published:2023-03-28

Abstract

Abstract:

【Objective】 The colonization effects of Piriformospora indica on Osmanthus fragrans under drought stress were analyzed. 【Method】 Two-year seedlings cultivated in pots with soil were used for this study. The experiment design was set up with the following parameters: inoculation (40 mL mycelium of P. indica) + drought (the soil water content is 20%-25% of the maximum field water capacity), inoculation + slight drought (45%-50% of the maximum field water capacity), inoculation + normal irrigation (65%-70% of the maximum field water capacity), control (40 mL inactivator) + drought, control + slight drought, and control + drought, six treatments in all. 【Result】 (1) Results showed that the colonization rate of P. indica was up to 81%. (2) Compared to the controls, the inoculated seedlings presented a better performance in plant height, stem diameter, and dry mass under both slight drought and drought stresses, especially the inoculation + drought groups, which showed significant or even extremely significant differences. (3) The protective enzyme activity of POD and SOD and the contents of proline and soluble sugar increased by 13.8%, 37.9%, 47.6% and 40.7%, respectively. (4) In addition, the MDA content decreased more quickly in the inoculation group than in the control group (control + drought). 【Conclusion】 This study found that the symbiosis of P. indica with O. fragrans played a positive role for O. fragrans seedlings in withstanding the negative effects of drought stress summer, most likely by stimulating the increase of SOD and POD enzyme activities and the significant accumulation of osmoregulatory substances. In contrast, inoculation with P. indica had no significant effect on the growth of O. fragrans under mild drought and normal water supply conditions.

Key words: Piriformospora indica, Osmanthus fragrans, drought stress, osmotic adjustment, drought resistance

CLC Number:

S685.13

MU Hongna, WANG Wei, FAN Lei, WU Chu, GUO Xiaohua, SUN Taoze. Effects of Piriformospora indica on growth and drought resistance in Osmanthus fragrans under water deficit stress[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(2): 101-106.

Figures/Tables 5

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Table 1

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干旱胁迫 drought stress	株高/cm plant height		地径/mm ground diameter		干质量/g dry mass
干旱胁迫 drought stress	未定殖 no	定殖 yes	未定殖 no	定殖 yes	未定殖 no	定殖 yes
干旱drought	22.30±0.23 b	25.20±0.33 a	3.60±0.05 a	3.99±0.07 b	8.39±0.05 a	11.09±0.06 b
轻度干旱slight drought	24.71±0.17 a	25.91±0.28 a	4.01±0.09 a	4.17±0.16 a	11.20±0.12 a	11.70±0.17 a
正常供水normal irrigation	26.89±0.05 a	26.70±0.01 a	4.60±0.16 a	4.74±0.03 a	13.07±0.26 a	12.90±0.20 a

Effects of Piriformospora indica on growth and drought resistance in Osmanthus fragrans under water deficit stress

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