Genetic diversity analysis of Lagerstroemia indica based on fluorescent SSR markers

WANG Zhiyi, LI Zhenfang, PENG Chan, CHEN Ying, ZHANG Xinye

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2023, Vol. 47 ›› Issue (2) : 61-69.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2023, Vol. 47 ›› Issue (2) : 61-69. DOI: 10.12302/j.issn.1000-2006.202203038

Genetic diversity analysis of Lagerstroemia indica based on fluorescent SSR markers

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Abstract

【Objective】 In this study, 239 germplasms of Lagerstroemia and one germplasm of Heimia were used to analyze the interspecific characteristics and genetic diversity of germplasm resources within L. indica, providing a scientific basis for collection, conservation and rational utilization of germplasm resources.【Method】 Genotypes of samples were identified based on 16 pairs of fluorescent primers; genotypes were read by the GeneMarker software. The characteristic loci of 239 germplasms of L. indica and one germplasm of H. myrtifolia were analyzed. The POPGENE, Cervus, NTSYS and GenAlEx software were used to estimate genetic diversity parameters, the cluster analysis and principal corrdinate analysis of 227 L. indica germplasms. 【Result】 Characteristic loci information of L. indica was the most abundant. Among the characteristic loci information reflected by 15 primers, L. indica all had unique loci that were different from the other six species, and some specific characteristic loci were not found in L. limii, L. excelsa and L. caudata. A total of 143 alleles were detected by 16 pairs of SSR(simple sequence repeat) fluorescent primers; there was an average of 8.938 alleles per locus and the average number of alleles per locus (Ne) was 3.600; Shannon’s Information Index (I) was 1.459, the average observed heterozygosity (Ho) was 0.577, the average expected heterozygosity (He) was 0.687, the average value of Nei’s genetic diversity index (H) was 0.685, and the average polymorphism information (PIC) in primers was 0.648. From the genetic diversity of 227 L. indica. based on the genetic distance between samples, a genetic cluster map was drawn and the samples were divided into 10 groups. The L. indica germplasm introduced from United States and wild L. indica germplasm from Hubei were relatively independent and clustered into different groups. The cluster analysis showed that L. indica germplasms were divided into 10 groups, among which the imported L. indica germplasm from the United States and the wild L. indica germplasm from Hubei were clustered into different groups. The principal corrdinate analysis showed that the 10 groups could be roughly divided into three regions, among which the wild L. indica germplasm from Hubei Province and the germplasm introduced from the United States were relatively clustered together.【Conclusion】 SSR markers can effectively reflect genetic diversity among L. indica germplasm resources, and can further optimize the collection and preservation of L. indica germplasm resources, build the core germplasm, create new varieties and provide a scientific basis for the rational development and utilization.

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Lagerstroemia indica / genetic diversity / simple sequence repeat (SSR) / clustering analysis

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WANG Zhiyi , LI Zhenfang , PENG Chan , et al . Genetic diversity analysis of Lagerstroemia indica based on fluorescent SSR markers[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2023, 47(2): 61-69 https://doi.org/10.12302/j.issn.1000-2006.202203038

References

List( Publishing order | Descend order by publishing year | Descend order by cited within ) Chart analysis
[1]
陈俊愉. 《中国花卉品种分类学》序言[J]. 中国园林, 1998, 14(5):21-22.
CHEN J Y. Preface to taxonomy of Chinese flower varieties[J]. J Chin Landsc Archit, 1998, 14(5):21-22.
Cited in this article [1]
[2]
乔东亚, 王鹏, 王淑安, 等. 基于SNP标记的紫薇遗传多样性分析[J]. 南京林业大学学报(自然科学版), 2020, 44(4):21-28.
QIAO D Y, WANG P, WANG S A, et al. Genetic diversity analysis of Lagerstroemia germplasm resources based on SNP markers[J]. J Nanjing For Univ (Nat Sci Ed), 2020, 44(4):21-28.DOI:10.3969/j.issn.1000-2006.202003075.
Cited in this article [1]
[3]
方文培, 张泽荣. 中国植物志[M]. 北京: 科学出版社, 1983.
FANG W P, ZHANG Z R. Flora reipublicae popularis sinicae[M]. Beijing: Science Press, 1983.
Cited in this article [3]
[4]
王金凤, 柳新红, 陈卓梅. 紫薇属植物育种研究进展[J]. 园艺学报, 2013, 40(9):1795-1804.
WANG J F, LIU X H, CHEN Z M. Research progress in breeding of Lagerstroemia plant[J]. Acta Hortic Sin, 2013, 40(9):1795-1804.DOI:10.16420/j.issn.0513-353x.2013.09.016.
Cited in this article [2]
[5]
罗雪梦, 王晓明, 曾慧杰, 等. 紫叶紫薇花芽分化及雌雄配子体发育的形态学研究[J]. 中南林业科技大学学报, 2022, 42(2):129-138.
LUO X M, WANG X M, ZENG H J, et al. Morphological study on floral bud differentiation and the development of female and male gametophytes in purple-leaf Lagerstroemia indica[J]. J Central South Univ For & Technol, 2022, 42(2):129-138.DOI:10.14067/j.cnki.1673-923x.2022.02.014.
Cited in this article [1]
[6]
陈燕, 李玉娟, 王莹, 等. 紫薇属植物研究进展[J]. 安徽农业科学, 2021, 49(4):7-9.
CHEN Y, LI Y J, WANG Y, et al. Research progress of Lagerstroemia plants[J]. J Anhui Agric Sci, 2021, 49(4):7-9.DOI:10.3969/j.issn.0517-6611.2021.04.003.
Cited in this article [2]
[7]
梁燕, 韩传明, 孙超, 等. 基于AFLP标记的薄壳山核桃种质资源遗传多样性分析[J]. 江苏林业科技, 2022, 49(1):1-7.
LIANG Y, HAN C M, SUN C, et al. Genetic diversity analysis of pecan germplasm resources based on AFLP markers[J]. J Jiangsu For Sci & Technol, 2022, 49(1):1-7.DOI:10.3969/j.issn.1001-7380.2022.01.001.
Cited in this article [1]
[8]
袁金玲, 马婧瑕, 钟远标, 等. 基于SSR标记的丛生竹杂种鉴定、遗传分析和指纹图谱构建[J]. 南京林业大学学报(自然科学版), 2021, 45(5):10-18.
YUAN J L, MA J X, ZHONG Y B, et al. SSR-based hybrid identification,genetic analyses and fingerprint development of hybridization progenies from sympodial bamboo (Bambusoideae,Poaceae)[J]. J Nanjing For Univ (Nat Sci Ed), 2021, 45(5):10-18.DOI:10.12302/j.issn.1000-2006.202012046.
Cited in this article [1]
[9]
杨卓, 杨晓杰, 付学鹏. 扎龙湿地10个不同居群芦苇的ITS序列分析[J]. 种子, 2021, 40(11):122-125.
YANG Z, YANG X J, FU X P. ITS sequence analysis of 10 different populations of Phragmites australis in Zhalong wetland[J]. Seed, 2021, 40(11):122-125.DOI:10.16590/j.cnki.1001-4705.2021.11.122.
Cited in this article [1]
[10]
DUARTE A B, GOMES W S, NIETSCHE S, et al. Genetic diversity between and within full sib families of Jatropha using ISSR markers[J]. Ind Crops Prod, 2018, 124:899-905.DOI:10.1016/j.indcrop.2018.08.066.
Cited in this article [1]
[11]
臧明月, 李璇, 方炎明. 基于SSR标记的白栎天然居群遗传多样性分析[J]. 南京林业大学学报(自然科学版), 2021, 45(1):63-69.
ZANG M Y, LI X, FANG Y M. Genetic diversity analysis among natural populations of Quercus fabri based on SSR markers[J]. J Nanjing For Univ (Nat Sci Ed), 2021, 45(1):63-69.DOI:10.12302/j.issn.1000-2006.202004049.
Cited in this article [1]
[12]
王亮, 韦继光, 葛春峰, 等. 基于SSR标记分析蓝莓品种‘蓝美1号’自由授粉子代遗传多样性及群体遗传结构[J]. 植物资源与环境学报, 2022, 31(3):35-43.
WANG L, WEI J G, GE C F, et al. Analysis on genetic diversity and population genetic structure of open-pollinated progenies of Vaccinium corymbosum ‘Lanmei 1’ based on SSR marker[J]. J Plant Resour Environ, 2022, 31(3):35-43.DOI: 10.3969/j.issn.1674-7895.2022.03.05.
Cited in this article [2]
[13]
王玲玲, 陈东亮, 黄丛林, 等. SSR分子标记技术在植物研究中的应用[J]. 安徽农业科学, 2017, 45(36):123-126,130.
WANG L L, CHEN D L, HUANG C L, et al. Application of SSR molecular marker technique in plant research[J]. J Anhui Agric Sci, 2017, 45(36):123-126,130.DOI:10.13989/j.cnki.0517-6611.2017.36.039.
Cited in this article [1]
[14]
WANG L H, ZHANG Y X, ZHU X D, et al. Development of an SSR-based genetic map in sesame and identification of quantitative trait loci associated with charcoal rot resistance[J]. Sci Rep, 2017, 7:8349.DOI:10.1038/s41598-017-08858-2.
Cited in this article [1]
[15]
CHIOU C Y, SHIH H C, TSAI C C, et al. The genetic relationships of Indian jujube (Ziziphus mauritiana Lam.) cultivars using SSR markers[J]. Heliyon, 2020, 6(10):e05078.DOI:10.1016/j.heliyon.2020.e05078.
Cited in this article [1]
[16]
罗芊芊, 赵明水, 肖德卿, 等. 基于SSR标记羊角槭子一代和子二代的遗传多样性分析[J]. 植物资源与环境学报, 2022, 31(5):66-73.
LUO Q Q, ZHAO M S, XIAO D Q, et al. Analysis on genetic diversity of first filial generation and second filial generation of Acer yangjuechi based on SSR markers[J]. J Plant Resour Environ, 2022, 31(5):66-73.DOI: 10.3969/j.issn.1674-7895.2022.05.08.
Cited in this article [1]
[17]
石纹豪, 黄振霖, 杨水英, 等. 重庆马铃薯致病疫霉(Phytophthora infestans)的SSR基因型分析[J]. 西南大学学报(自然科学版), 2014, 36(12):36-41.
SHI W H, HUANG Z L, YANG S Y, et al. SSR genotypic analysis of Phytophthora infestans on potato plants in Chongqing[J]. J Southwest Univ (Nat Sci Ed), 2014, 36(12):36-41.DOI:10.13718/j.cnki.xdzk.2014.12.007.
Cited in this article [1]
[18]
POOLER M R. Molecular genetic diversity among 12 clones of Lagerstroemia fauriei revealed by AFLP and RAPD markers[J]. HortScience, 2003, 38(2):256-259.DOI:10.21273/hortsci.38.2.256.
Cited in this article [1]
[19]
RINEHART T A, POUNDERS C T. Estimating diversity among lagerstroemia species and hybrids using SSR markers[J]. Acta Hortic, 2010(885):285-290.DOI:10.17660/actahortic.2010.885.39.
Cited in this article [2]
[20]
WANG X W, WADL P A, POUNDERS C, et al. Evaluation of genetic diversity and pedigree within crapemyrtle cultivars using simple sequence repeat markers[J]. J Amer Soc Hort Sci, 2011, 136(2):116-128.DOI:10.21273/jashs.136.2.116.
Cited in this article [2]
[21]
CAI M, PAN H T, WANG X F, et al. Development of novel microsatellites in Lagerstroemia indica and DNA fingerprinting in Chinese Lagerstroemia cultivars[J]. Sci Hortic, 2011, 131:88-94.DOI:10.1016/j.scienta.2011.09.031.
Cited in this article [2]
[22]
HE D, LIU Y, CAI M, et al. Genetic diversity of Lagerstroemia (Lythraceae) species assessed by simple sequence repeat markers[J]. Genet Mol Res, 2012, 11(3):3522-3533.DOI:10.4238/2012.September.26.9.
Cited in this article [2]
[23]
彭婵, 李振芳, 马林江, 等. 紫薇属种间杂种SSR分子鉴定与遗传分析[J]. 北方园艺, 2020(13):83-90.
PENG C, LI Z F, MA L J, et al. Identification and genetic analysis in Lagerstroemia interspecific hybrids using SSR markers[J]. North Hortic, 2020(13):83-90.DOI:10.11937/bfyy.20193171.
Cited in this article [6]
[24]
张海燕, 樊军锋, 郑涛. 18份杨树资源的遗传多样性分析[J]. 西北农林科技大学学报(自然科学版), 2020, 48(12):47-54,63.
ZHANG H Y, FAN J F, ZHENG T. Genetic diversity analysis of 18 poplar resources[J]. J Northwest A & F Univ (Nat Sci Ed), 2020, 48(12):47-54,63.DOI:10.13207/j.cnki.jnwafu.2020.12.006.
Cited in this article [1]
[25]
苏晓娜, 王惠梅, 黄雪雯, 等. 利用SSR标记分析东北野生菰遗传多样性和遗传结构[J]. 江西农业大学学报, 2018, 40(3):579-589.
SU X N, WANG H M, HUANG X W, et al. Genetic diversity and genetic structure of Zizania latifolia Griseb(Turcz) from northeast China based on SSR analysis[J]. Acta Agric Univ Jiangxiensis, 2018, 40(3):579-589.DOI:10.13836/j.jjau.2018075.
Cited in this article [1]
[26]
李博, 潘文婷, 李康琴, 等. 三种常用亲本分析软件效率的比较:以鹅掌楸属树种控制授粉子代亲本分析为例[J]. 分子植物育种, 2013, 11(2):241-248.
LI B, PAN W T, LI K Q, et al. A comparison among three softwares of parentage analysis (CERVUS,COLONY and PAPA):a case study on parentage analysis of control pollination progenies in Liriodendron[J]. Mol Plant Breed, 2013, 11(2):241-248.DOI:10.3969/mpb.011.000241.
Cited in this article [1]
[27]
李欣. 居群遗传分析软件:GENALEX 6简介[J]. 中国野生植物资源, 2008, 27(4):59-62.
LI X. Introduction to a population genetic software: GENALEX 6[J]. Chin Wild Plant Resour, 2008, 27(4):59-62.DOI:10.3969/j.issn.1006-9690.2008.04.017.
Cited in this article [1]
[28]
赵盼, 栗丹阳, 马锦林, 等. 油料树种千年桐的SSR标记开发、遗传多样性与群体结构分析[J]. 北京林业大学学报, 2021, 43(11):50-61.
ZHAO P, LI D Y, MA J L, et al. SSR marker development,genetic diversity and population structure analysis in oil tree species Vernicia montana[J]. J Beijing For Univ, 2021, 43(11):50-61.DOI:10.12171/j.1000-1522.20210054.
Cited in this article [1]
[29]
乔中全, 王晓明, 李永欣, 等. 38个紫薇品种亲缘关系的ISSR分析[J]. 浙江农业学报, 2019, 31(4):565-571.
QIAO Z Q, WANG X M, LI Y X, et al. Phylogenetic relationships among 38 cultivars of Lagerstroemia indica based on ISSR molecular markers[J]. Acta Agric Zhejiangensis, 2019, 31(4):565-571.DOI:10.3969/j.issn.1004-1524.2019.04.08.
Cited in this article [2]
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