Comparative analysis of different types of callus in moso bamboo

HUANG Biyun, ZHUO Renying, QIAO Guirong

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2023, Vol. 47 ›› Issue (6) : 141-149.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2023, Vol. 47 ›› Issue (6) : 141-149. DOI: 10.12302/j.issn.1000-2006.202203062

Comparative analysis of different types of callus in moso bamboo

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Abstract

【Objective】 The proliferation ability and differentiation potential of different types of moso bamboo callus were compared with reference to three aspects: cell morphology, cell cycle phase distribution ratio and gene expression. The results provided a basis for selecting suitable callus for in vitro regeneration of moso bamboo. 【Method】 Three different types of callus were selected according to appearance and texture by using the immature embryo of moso bamboo as an explant to induce callus. The proliferation and regeneration ability of three types of moso bamboo callus were investigated by paraffin section and flow cytometry. Real-time PCR (qRT-PCR) was used to analyze the expression of genes related to embryonic maintenance and growth regulation in different callus types, and to select candidate genes that could be used to improve regeneration efficiency in the future. 【Result】 The type Ⅱ of white and dense callus had a large number of embryogenic meristem cells on the surface and inside. The proportion of cells in stage S and G2 in this callus type was significantly higher than that in the other types of callus, reaching 8.78% and 8.95% of the total number of cells, respectively. At the same time, the expression of two PeWUS was higher in type Ⅱ and type Ⅱ callus with strong embryogenesis, and the expression of seven PeGRF genes was the highest in type Ⅱ callus. 【Conclusion】 There were differences in proliferation and regeneration ability of different types of moso bamboo callus. The type Ⅱ of white dense callus with stronger proliferation and regeneration ability was more suitable as a tissue culture material. The selected differential genes could be used as candidate genes to improve regeneration efficiency of moso bamboo.

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moso bamboo / callus / histology / cell proliferation / gene expression

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HUANG Biyun , ZHUO Renying , QIAO Guirong. Comparative analysis of different types of callus in moso bamboo[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2023, 47(6): 141-149 https://doi.org/10.12302/j.issn.1000-2006.202203062

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