An overview of high-throughput sequencing techniques applied on Bursaphelenchus xylophilus

DING Xiaolei, ZHANG Yue, LIN Sixi, YE Jianren

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (4) : 1-7.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (4) : 1-7. DOI: 10.12302/j.issn.1000-2006.202204039

An overview of high-throughput sequencing techniques applied on Bursaphelenchus xylophilus

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Abstract

As of 2022, four decades have elapsed since the pine wilt disease entered China. The disease caused by the pine wood nematode (Bursaphelenchus xylophilus) poses a major threat to all pine forests in Asia and Europe. Since the announcement of the first B. xylophilus genome assembly in 2011, high-throughput sequencing techniques have been widely used in all aspects of relevant studies. They have facilitated researchers greatly in further exploring the molecular pathogenesis of B. xylophilus from gene functions, non-coding RNA, transcriptomics and genomics. With rapid developments in sequencing techniques, we suggest researchers to use spatial transcriptomics and single cell sequencing methods for identifying long non-coding RNAs, fusion genes, circular RNAs, as well as gene methylations, RNA editings and structural variations with potential regulatory roles. As for genomics, future studies could focus on gene family expansion, genome wide association and quantitative trait locus studies to screen the gene and loci highly associated with the pathogenicity and reproductivity of B. xylophilus and reveal the adaptation mechanisms among different nematode populations during the evolution.

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Bursaphelenchus xylophilus / high-throughput sequencing / transcriptomics / genomics

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DING Xiaolei , ZHANG Yue , LIN Sixi , et al. An overview of high-throughput sequencing techniques applied on Bursaphelenchus xylophilus[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2022, 46(4): 1-7 https://doi.org/10.12302/j.issn.1000-2006.202204039

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