Expressional profiling of circRNAs under PEG and NaCl stresses in germinated moso bamboo seeds

WANG Xiaojing, WANG Tao, YANG Kai, LI Lubin

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2023, Vol. 47 ›› Issue (6) : 17-24.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2023, Vol. 47 ›› Issue (6) : 17-24. DOI: 10.12302/j.issn.1000-2006.202204063

Expressional profiling of circRNAs under PEG and NaCl stresses in germinated moso bamboo seeds

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Abstract

【Objective】 Circular RNAs (circRNAs) are a class of endogenous non-coding RNA produced by reverse alternative splicing of transcripts, which play crucial roles in plant growth and development. This study aimed to identify circRNAs in germinated seeds of moso bamboo (Phyllostachys edulis), and investigate their expression under drought and salt stress conditions, providing a foundation for exploring the molecular regulation mechanism of bamboo seed germination under abiotic stress resistance. 【Method】 polyethylene glycol (PEG) 6000 and NaCl were used to simulate drought and salt stress, respectively. The strand specific libraries were constructed for moso bamboo seeds samples at the seed coat rupture stage under treatments of H2O, 10% (mass fraction) PEG, 15% PEG, 50 and 100 mol/L NaCl. High throughout sequencing and biological information analysis were used to identify and analysis the expressional pattern of circRNAs. 【Result】 A total of 1 446 circRNAs were identified in all samples, with 76.34% of circRNAs originating from exon region; The circRNAs with the highest expression levels in bamboo germinating seeds under different treatments were identified as plant_ circ_ 0001703, plant_ circ_ 0001728, plant_ circ_ 0000358, plant_ circ_ 0001236 and plant_ circ_ 0001728;Compared with control, 524, 505, 467 and 474 circRNAs were significantly differentially expressed in germinating seeds under the four stress treatments. The original genes of differentially expressed circRNAs were significantly enriched in various GO and KEGG pathways. 【Conclusion】 A total of 1 446 circRNAs were found to be expressed in moso bamboo germinating seeds, with 1 056 circRNAs responding to drought or salinity stresses during seed germination. Genes involved in acylhydrolase enzyme activity and sphingolipid metabolism pathways may play a crucial role in regulating bamboo seed germination under PEG or NaCl stress conditions.

Key words

moso bamboo / germination / polyethylese glycol (PEG) / NaCl / circRNAs

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WANG Xiaojing , WANG Tao , YANG Kai , et al. Expressional profiling of circRNAs under PEG and NaCl stresses in germinated moso bamboo seeds[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2023, 47(6): 17-24 https://doi.org/10.12302/j.issn.1000-2006.202204063

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