Morphological and ultrastructural characteristics of root nodules and rhizobium of Dalbergia odorifera

doi:10.12302/j.issn.1000-2006.202205001

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2023, Vol. 47 ›› Issue (5): 259-267.doi: 10.12302/j.issn.1000-2006.202205001

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Morphological and ultrastructural characteristics of root nodules and rhizobium of Dalbergia odorifera

DENG Jiazhen(), YE Shaoming^*(), LIN Mingye, LAN Yahui, YAN Yu, FAN Rongyuan, PAN Cailing

College of Forestry, Guangxi Key Laboratory of Forest Ecology and Conservation, Guangxi University, Nanning 530004, China

Received:2022-04-30 Revised:2023-06-02 Online:2023-09-30 Published:2023-10-10

Abstract

Abstract:

【Objective】This study aims to elucidate the morphological characteristics, histological characteristics, and ultrastructure of Dalbergia odorifera root nodules and clarify the ultrastructure of rhizobium. The results from this study can provide an intuitive and precise primary data set for the classification of leguminous plant nodules.【Method】The paraffin sections, ultrathin sections and field emission scanning electron microscopy (FESEM) samples were all prepared from D. odorifera root nodules. Optical microscopy (OM) was used to observe the morphology and microscopic structure of nodules, while transmission electron microscopy (TEM) and FESEM were used to observe the ultrastructure of root nodule cells and rhizobium. Additionally, the leghemoglobin content of nodules in different periods was determined, and the relationship between root nodules structure and nitrogen fixation function was analyzed.【Result】(1) Morphological characteristics: from day one to day 60, the shape of D. odorifera root nodules gradually differentiated from round into oval, barbell and irregular oval, and the color changed from yellowish white to yellowish brown. The growing part of nodules gradually spread from the lateral root and base to the principal root. During the development of root nodules, the leghemoglobin content initially rose, then declined, with a peak at age of 3-5 d. (2) Histological traits: the root nodules of D. odorifera generally contained two to three infected areas. The vascular bundles were distributed around the infected areas, and the ringed vessels inside were connected by the end wall. (3) Ultrastructures: under TEM observation, the infected cells were significantly different from the uninfected cells. The volume of infected cells was enlarged without vacuoles and contained multiple vesicles with membranous structure. The vesicles were covered by bacteroids, which were differentiated from rhizobium, and organelles such as mitochondria and endoplasmic reticulum were squeezed to the edge of the cell wall. The uninfected cells were almost filled with vacuoles, and the cell wall was thick and uneven. Starch grains and some plastids existed around the nucleus. The morphologically diverse bacteroids have cell membranes and walls and contain polyphosphate (PP) particles and poly-β-hydroxybutyrate (PHB) particles. There were two membrane synthesis states in the infected cells of D. odorifera root nodules. In one, the membrane vesicles participated in the synthesis of peribacteroid membranes; in the other, a flocculent membrane appeared near the peribacteroid membranes and allowed the peribacteroid membrane to dissociate. FESEM showed a large number of long rod-like rhizobium intertwined and multiple peribacteroid membranes bonded together in a continuous shape to form a tight membrane system.【Conclusion】The D. odorifera root nodules are “determinate nodules”. The nodule age can significantly affect the leghemoglobin content in D. odorifera root nodules. The D. odorifera root nodules contain well-developed vascular tissues, and there are a large number of bacteroids that contain PP and PHB particles with an energy storage function as well as a strong peribacteroid membrane system. These structures provide supports to the nitrogen fixation function of D. odorifera root nodules.

Key words: Dalbergia odorifera, root nodule, rhizobium, ultrastructure, bacteroid, symbiotic nitrogen fixation

CLC Number:

S718

DENG Jiazhen, YE Shaoming, LIN Mingye, LAN Yahui, YAN Yu, FAN Rongyuan, PAN Cailing. Morphological and ultrastructural characteristics of root nodules and rhizobium of Dalbergia odorifera[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(5): 259-267.

Figures/Tables 6

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瘤龄/d growing day	根瘤形状 nodules shape	根瘤颜色 nodules color	着生部位 growing part	根瘤长径/mm nodules size	豆血红蛋白含量/(mg·g^-1) leghemoglobin content
1	圆形	黄白色	侧根、侧根基部	< 1	527.06±1.11 b
3~5	圆形	淡黄色	侧根、侧根基部	[1,2)	716.08±15.70 a
7~10	圆形	黄色	主根、侧根	[2,3)	522.16±17.79 b
11~20	球形	黄褐色	主根、侧根	[3,4)	419.61±36.19 c
21~60	杠铃形、长椭圆形	黄褐色	主根、侧根	[4,5)	189.80±28.37 d

Morphological and ultrastructural characteristics of root nodules and rhizobium of Dalbergia odorifera

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