Extraction technology and antioxidant activity analyses of total polyphenols from Xanthoceras sorbifolia leaves

doi:10.12302/j.issn.1000-2006.202205009

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2023, Vol. 47 ›› Issue (5): 129-137.doi: 10.12302/j.issn.1000-2006.202205009

Previous Articles Next Articles

Extraction technology and antioxidant activity analyses of total polyphenols from Xanthoceras sorbifolia leaves

SU Jinghan¹(), WANG Gaiping¹^,^*(), LIU Yuhua², QI Ya¹, PENG Daqing³, LI Shouke⁴, CAO Fuliang¹

1. Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China
2. Jiangsu Vocational and Technical College of Agriculture and Forestry, Jurong 212400, China
3. Jiangsu Yancheng Forest Farm, Yancheng 224057, China
4. Shandong Woqi Agricultural Development Co., Ltd., Weifang 262106, China

Received:2022-05-07 Revised:2022-09-26 Online:2023-09-30 Published:2023-10-10

Abstract

Abstract:

【Objective】This research aims to explore the best extraction process of total polyphenols from Xanthoceras sorbifolia leaves, compare the content and antioxidant activity of total polyphenols from different sources, and provide a reference for the development and utilization of X. sorbifolia leaves. 【Method】The total polyphenols of X. sorbifolia leaves from 15 sources were extracted by ultrasonic assisted response surface method, and the content of total polyphenols in X. sorbifolia leaves was determined by Folin-iocaileu colorimetry. The antioxidant capacity of X. sorbifolia leaves was evaluated by measuring the scavenging power of total polyphenols on the DPPH free radical and the antioxidant capacity of FRAP method. 【Result】Through single factor experiments, the optimum extraction process of total polyphenols from X. sorbifolia leaves was obtained with ethanol concentration 70%, solid-liquid ratio 1∶30 g/mL and extraction temperature 60 ℃. The extraction rate of total polyphenols from X. sorbifolia leaves was optimized according to Box-Behnken response surface method. The optimum extraction conditions were as follows: ethanol concentration 60%, solid-liquid ratio 1∶30 g/mL, extraction time 43 min and extraction temperature 70 ℃. The average content of total polyphenols in 15 sources was 36.34 mg/g, of which the content of total polyphenols in X. sorbifolia leaves in Yinchuan, Ningxia was the highest, 42.37 mg/g. The antioxidant capacity of total polyphenols in X. sorbifolia leaves from different sources was different. The DPPH free radical scavenging rate of total polyphenols in X. sorbifolia leaves from 15 sources ranged from 64.33% to 92.65%, and the DPPH free radical scavenging rate of Yinchuan provenance in Ningxia was the highest, 92.65%. The total antioxidant capacity of total polyphenols in X. sorbifolia leaves from 15 sources ranged from 0.71 to 1.53 mol/g, and the total antioxidant capacity from Ledu provenance in Qinghai was the strongest, 1.53 mol/g. The APC index of Ledu is the highest, 99.73%; Xinjiang Changji has the lowest APC index, only 62.86%.The content of total polyphenols in X. sorbifolia leaves was positively correlated with the antioxidant capacity of its extract. 【Conclusion】The optimum extraction conditions of total polyphenols from X. sorbifolia leaves were ethanol concentration 60%, solid-liquid ratio 1∶30 g/mL, extraction time 43 min and extraction temperature 70 ℃. The total polyphenol contents of X. sorbifolia leaves from Yinchuan in Ningxia and Ledu in Qinghai are high and the antioxidant capacity are strong.

Key words: Xanthoceras sorbifolia, total polyphenols, antioxidant activity, response surface, ultrasonic

CLC Number:

SU Jinghan, WANG Gaiping, LIU Yuhua, QI Ya, PENG Daqing, LI Shouke, CAO Fuliang. Extraction technology and antioxidant activity analyses of total polyphenols from Xanthoceras sorbifolia leaves[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(5): 129-137.

Figures/Tables 7

Table 1

Table 2

Table 3

Fig. 1

Fig. 2

Table 4

Table 5

References 47

[1]	周荣汉. 中药资源学[M]. 北京: 中国医药科技出版社, 1993:331-339.
	ZHOU R H. Resource science of Chinese medicinal materials[M]. Beijing: Medical Science and Technology Press, 1993:331-339.
[2]	张育松, 郭素枝, 韦勤, 等. 文冠果叶茶制作工艺及保健功效[J]. 福建茶叶, 2016, 38(3):26,79.
	ZHANG Y S, GUO S Z, WEI Q, et al. Preparation technology and health care effect of Xanthoceras sorbifolia leaf tea[J]. Tea Fujian, 2016, 38(3):26,79.DOI: 10.3969/j.issn.1005-2291.2016.03.015.
[3]	JI X F, CHI T Y, LIU P, et al. The total triterpenoid saponins of Xanthoceras sorbifolia improve learning and memory impairments through against oxidative stress and synaptic damage[J]. Phyto Medicine, 2017, 25:15-24.DOI: 10.1016/j.phymed.2016.12.009.
[4]	ZHANG Y, MA J N, MA C L, et al. Simultaneous quantification of ten constituents of Xanthoceras sorbifolia Bunge using UHPLC-MS methods and evaluation of their radical scavenging,DNA scission protective,and α-glucosidase inhibitory activities[J]. Chin J Nat Med, 2015, 13(11):873-880.DOI: 10.1016/S1875-5364(15)30092-3.
[5]	YANG N, JIN Y M, LI D D, et al. Development of a series-parallel reaction system for rapid aqueous extraction of seed oil from yellow horn at mild condition[J]. Food Bioprod Process, 2017, 102:144-152.DOI: 10.1016/j.fbp.2016.12.010.
[6]	姜楠, 王蒙, 韦迪哲, 等. 植物多酚类物质研究进展[J]. 食品安全质量检测学报, 2016, 7(2):439-444.
	JIANG N, WANG M, WEI D Z, et al. Research progress on plant polyphenols[J]. J Food Saf & Qual, 2016, 7(2):439-444.DOI: 10.19812/j.cnki.jfsq11-5956/ts.2016.02.008.
[7]	田旭坤. 植物多酚的提取工艺研究[D]. 哈尔滨: 哈尔滨理工大学, 2019.
	TIAN X K. Study on extraction technology of plant polyphenols[D]. Harbin: Harbin University of Science and Technology, 2019.
[8]	田富林, 黄文晶, 王展, 等. 植物多酚提取研究进展[J]. 食品与机械, 2020, 36(9):211-216.
	TIAN F L, HUANG W J, WANG Z, et al. Research progress on the extraction of plant polyphenols[J]. Food & Mach, 2020, 36(9):211-216.DOI: 10.13652/j.issn.1003-5788.2020.09.038.
[9]	董科, 冷云, 何方婷, 等. 植物多酚及其提取方法的研究进展[J]. 食品工业科技, 2019, 40(2):326-330.
	DONG K, LENG Y, HE F T, et al. Research progress of polyphenol and extraction methods in plants[J]. Sci Technol Food Ind, 2019, 40(2):326-330.DOI: 10.13386/j.issn1002-0306.2019.02.057.
[10]	吕艳伟, 王光全, 孟庆杰, 等. 干旱对毛白杨不同品种幼苗生长和抗氧化系统的影响[J]. 黑龙江农业科学, 2013(6):60-64.
	LYU Y W, WANG G Q, MENG Q J, et al. Effects of drought on seedlings growth and antioxidant system of different varieties of Populus tomentosa Carr[J]. Heilongjiang Agric Sci, 2013(6):60-64.DOI: 10.3969/j.issn.1002-2767.2013.06.017.
[11]	丁建英, 王晓飞, 张丽, 等. 枇杷叶多酚超声波辅助提取工艺优化及其抗氧性分析[J]. 南方农业学报, 2018, 49(2):340-347.
	DING J Y, WANG X F, ZHANG L, et al. Ultrasonic assisted extraction of polyphenol from loquat leaves and its antioxidant activity[J]. J South Agric, 2018, 49(2):340-347.DOI: 10.3969/j.issn.2095-1191.2018.02.21.
[12]	王翔, 彭胜, 彭密军. 杜仲叶总多酚超声波-微波辅助提取及其抗氧化活性研究[J]. 林产化学与工业, 2018, 38(5):85-92.
	WANG X, PENG S, PENG M J. Extraction of total polyphenols from Eucommia ulmoides oliver leaves assisted by ultrasound-microwave and their antioxidant activity[J]. Chem Ind For Prod, 2018, 38(5):85-92.DOI: 10.3969/j.issn.0253-2417.2018.05.012.
[13]	李国倩, 秦天, 黄蓉, 等. 文冠果果壳醇提物不同部位总多酚含量及其抗氧化活性研究[J]. 中国医药导报, 2018, 15(34):27-31,36.
	LI G Q, QIN T, HUANG R, et al. Research on total polyphenol content of different ethanol fractions from Xanthoceras sorbifolia husks and the antioxidant activity[J]. China Med Her, 2018, 15(34):27-31,36.
[14]	郝倩, 段力歆, 马挺军, 等. 超声辅助提取文冠果叶总黄酮的工艺研究[J]. 食品工业科技, 2013, 34(16):295-300.
	HAO Q, DUAN L X, MA T J, et al. Study on technology of extracting total flavones from Xanthoceras sorbifolia Bunge leaves by ultrasonic wave[J]. Sci Technol Food Ind, 2013, 34(16):295-300.DOI: 10.13386/j.issn1002-0306.2013.16.034.
[15]	张严磊, 施欢贤, 雷莉妍, 等. 文冠果叶多糖超声辅助提取工艺及其药效学初步研究[J]. 中国现代中药, 2016, 18(12):1636-1640.
	ZHANG Y L, SHI H X, LEI L Y, et al. Study on ultrasonic assisted extraction technology and preliminary pharmacodynamics research of polysaccharide from Xanthoceras sorbifolia leaf[J]. Mod Chin Med, 2016, 18(12):1636-1640.DOI: 10.13313/j.issn.1673-4890.2016.12.023.
[16]	王桃云, 邱业先, 汪金莲, 等. 响应面法优化银杏叶多酚提取工艺及对脲酶抑制活性的研究[J]. 化学研究与应用, 2013, 25(7):999-1005.
	WANG T Y, QIU Y X, WANG J L, et al. Optimisation of extraction conditions for polyphenols from the leaves of Ginkgo biloba by response surface methodology and their inhibition on soil urease activity[J]. Chem Res Appl, 2013, 25(7):999-1005.DOI: 10.3969/j.issn.1004-1656.2013.07.014.
[17]	李衍方, 高彩霞, 高阳, 等. 栾树叶总多酚的超声波辅助提取工艺及其抗氧化活性[J]. 生物质化学工程, 2016, 50(3):27-34. doi: 10.3969/j.issn.1673-5854.2016.03.005
	LI Y F, GAO C X, GAO Y, et al. Ultrasonic-assisted extraction and antioxidant activity of polyphenols from Koelreuteria paniculata Laxm.leaves[J]. Biomass Chem Eng, 2016, 50(3):27-34.DOI: 10.3969/j.issn.1673-5854.2016.03.005.
[18]	傅予, 王宏, 张岩, 等. Box-Behnken设计-响应面法优化番石榴叶总黄酮提取工艺及总黄酮对糖尿病小鼠糖耐量的影响研究[J]. 中国药房, 2018, 29(1):49-53.
	FU Y, WANG H, ZHANG Y, et al. Optimization of extraction technology of total flavonoids Psidium guajava leaves by Box-Behnken design-response surface method and the effects of total flavonoids on glucose tolerance in diabetic model mice[J]. China Pharm, 2018, 29(1):49-53.DOI: 10.6039/j.issn.1001-0408.2018.01.13.
[19]	王慧芳, 赵飞燕, 刘勇军, 等. 文冠果叶总黄酮微波辅助酶提取工艺的优化及其抗氧化、抑菌活性[J]. 中成药, 2020, 42(2):290-296.
	WANG H F, ZHAO F Y, LIU Y J, et al. Microwave-assisted enzyme extraction process optimization and antioxidant,bacteriostasis activities of total flavonoids from Xanthoceras sorbifolium leaves[J]. Chin Tradit Pat Med, 2020, 42(2):290-296.DOI: 10.3969/j.issn.1001-1528.2020.02.004.
[20]	国家市场监督管理总局, 国家标准化管理委员会. 茶叶中茶多酚和儿茶素类含量的检测方法:GB/T 8313—2018[S]. 北京: 中国标准出版社, 2018.
[21]	张怡评, 杨婷, 洪专, 等. 青钱柳多酚提取工艺及抗氧化活性[J]. 食品工业, 2021, 42(6):212-216.
	ZHANG Y P, YANG T, HONG Z, et al. Extraction and antioxidant activities of polyphenols in Cyclocarya paliurus[J]. Food Ind, 2021, 42(6):212-216.
[22]	陈克克, 强毅. 响应面法优化超声波辅助北苍术多酚提取工艺及其DPPH自由基清除能力研究[J]. 西北大学学报(自然科学版), 2018, 48(1):78-84.
	CHEN K K, QIANG Y. Optimization of ultrasonic assisted extraction of polyphenols from Atractylodes chinensis(DC.) Koidz.using response surface methodology and evaluation of its antioxidant activity[J]. J Northwest Univ (Nat Sci Ed), 2018, 48(1):78-84.DOI: 10.16152/j.cnki.xdxbzr.2018-01-013.
[23]	廖友媛. 银杏叶与茶叶抗氧化活性的比较[J]. 江苏农业科学, 2009, 37(2):248-250.
	LIAO Y Y. Comparison of antioxidant activity between Ginkgo biloba leaves and tea leaves[J]. Jiangsu Agric Sci, 2009, 37(2):248-250.DOI: 10.3969/j.issn.1002-1302.2009.02.104.
[24]	陈琛, 蔺蓓蓓, 苏鹏超, 等. 不同产地红天麻多酚组成及体外抗氧化活性研究[J]. 西北农林科技大学学报(自然科学版), 2021, 49(12):144-154.
	CHEN C, LIN B B, SU P C, et al. Phenolic compositions and in vitro antioxidant activities of Gastrodia elata Bl.f.elata from different habitats in China[J]. J Northwest A & F Univ (Nat Sci Ed), 2021, 49(12):144-154.DOI: 10.13207/j.cnki.jnwafu.2021.12.017.
[25]	李鑫鑫, 徐尤美, 陈琛, 等. 天麻不同极性成分的体外抗氧化综合评价[J]. 食品科技, 2018, 43(7):66-71.
	LI X X, XU Y M, CHEN C, et al. Antioxidant activity of different solvent extracts from Gastrodia elata Bl[J]. Food Sci Technol, 2018, 43(7):66-71.DOI: 10.13684/j.cnki.spkj.2018.07.013.
[26]	陈建福, 林洵, 陈俊尧, 等. 响应面法优化超声波辅助提取柚叶多酚的工艺研究[J]. 河南工业大学学报(自然科学版), 2016, 37(3):47-54.
	CHEN J F, LIN X, CHEN J Y, et al. Optimization of ultrasonic-assisted extraction of polyphenols from pomelo leaves using response surface methodology[J]. J Henan Univ Technol (Nat Sci Ed), 2016, 37(3):47-54.DOI: 10.16433/j.cnki.issn1673-2383.2016.03.009.
[27]	陈建福, 林洵, 许商荣, 等. 超声波辅助法提取白兰叶多酚的工艺研究[J]. 中国食品添加剂, 2016(7):150-156.
	CHEN J F, LIN X, XU S R, et al. Ultrasonic-assisted extraction of polyphenols from Michelia alba leaves[J]. China Food Addit, 2016(7):150-156.DOI: 10.3969/j.issn.1006-2513.2016.07.016.
[28]	代燕丽, 沈维治, 廖森泰, 等. 响应面法优化超声波辅助提取桑叶多酚工艺[J]. 热带作物学报, 2016, 37(8):1588-1594.
	DAI Y L, SHEN W Z, LIAO S T, et al. Optimization of ultrasonic-assisted extraction process of mulberry polyphenols using response surface methodology[J]. Chin J Trop Crops, 2016, 37(8):1588-1594.DOI: 10.3969/j.issn.1000-2561.2016.08.023.
[29]	孔祥佳, 刘廉荣. 超声波乙醇浸提法提取橄榄叶总多酚工艺的研究[J]. 福建中医药, 2017, 48(2):34-37.
	KONG X J, LIU L R. Study on extraction technology of total polyphenols from olive leaves by ultrasonic ethanol extraction[J]. Fujian J Tradit Chin Med, 2017, 48(2):34-37.DOI: 10.13260/j.cnki.jfjtcm.011331.
[30]	沈伟, 张一文. 乌药叶多酚提取工艺优化及其对金黄色葡萄球菌的抑菌作用[J]. 食品安全质量检测学报, 2022, 13(4):1256-1263.
	SHEN W, ZHANG Y W. Optimization of extraction technology of polyphenols from leaves of Lindera aggregata and its antibacterial effect against Staphylococcus aureus[J]. J Food Saf & Qual, 2022, 13(4):1256-1263.DOI: 10.19812/j.cnki.jfsq11-5956/ts.2022.04.041.
[31]	楠极, 李远志. 响应面法优化番石榴叶多酚的超声提取工艺[J]. 食品工业科技, 2016, 37(22):300-304.
	NAN J, LI Y Z. Optimization of ultrasonic extraction of polyphenol from guava leaf using response surface methodology[J]. Sci Technol Food Ind, 2016, 37(22):300-304.DOI: 10.13386/j.issn1002-0306.2016.22.050.
[32]	施伟梅, 陈建福, 王妙飞, 等. 响应面法优化红花紫荆叶中多酚的超声波辅助提取工艺[J]. 粮油食品科技, 2014, 22(5):38-43.
	SHI W M, CHEN J F, WANG M F, et al. Optimization of ultrasonic-assisted extraction of polyphenols from B.blakeana Dunn leaves by response surface methodology[J]. Sci Technol Cereals Oils Foods, 2014, 22(5):38-43.DOI: 10.16210/j.cnki.1007-7561.2014.05.020.
[33]	陈亮, 李医明, 陈凯先, 等. 植物多酚类成分提取分离研究进展[J]. 中草药, 2013, 44(11):1501-1507.
	CHEN L, LI Y M, CHEN K X, et al. Research progress in extraction and isolation of plant polyphenols[J]. Chin Tradit Herb Drugs, 2013, 44(11):1501-1507.DOI: 10.7501/j.issn.0253-2670.2013.11.027.
[34]	贺银菊, 张旋俊, 杨再波, 等. 响应面优化紫果西番莲叶多酚超声辅助提取工艺及其抗氧化活性[J]. 食品工业科技, 2021, 42(1):211-216,226.
	HE Y J, ZHANG X J, YANG Z B, et al. Optimization of ultrasonic-assisted extraction of polyphenols in the purple passionfruit leaves by response surface methodology and its antioxidant activity[J]. Sci Technol Food Ind, 2021, 42(1):211-216,226.DOI: 10.13386/j.issn1002-0306.2020030146.
[35]	贺银菊, 张旋俊, 杨再波, 等. 响应面优化艾叶多酚提取工艺及抗氧化活性研究[J]. 食品科技, 2020, 45(6):278-284.
	HE Y J, ZHANG X J, YANG Z B, et al. Antioxidant activity and optimization of extraction of polyphenols in the Artemisia argyi by response surface methodology[J]. Food Sci Technol, 2020, 45(6):278-284.DOI: 10.13684/j.cnki.spkj.2020.06.049.
[36]	张健, 吕蒙蒙, 谢亚聪, 等. 响应面杉木叶片多酚提取工艺及其优化[J]. 福建农林大学学报(自然科学版), 2019, 48(6):765-770.
	ZHANG J, LÜ M M, XIE Y C, et al. Optimization of extraction process of polyphenols from Chinese fir leaves by response surface methodology[J]. J Fujian Agric For Univ (Nat Sci Ed), 2019, 48(6):765-770.DOI: 10.13323/j.cnki.j.fafu(nat.sci.).2019.06.013.
[37]	牛红军, 李军, 李建文, 等. 超声技术在植物多酚提取中的应用进展[J]. 农学学报, 2014, 4(7):92-95.
	NIU H J, LI J, LI J W, et al. The progress in applications of ultrasonic technique in extraction of polyphenols from plant[J]. J Agric, 2014, 4(7):92-95. doi: 10.11924/j.issn.2095-4050.2014-xb0089
[38]	刘杨, 赵婧, 梁莉, 等. 响应面优化蒲公英多酚超声波辅助乙醇提取工艺及其抗氧化性[J]. 食品工业科技, 2017, 38(2):287-292.
	LIU Y, ZHAO J, LIANG L, et al. Optimization of ultrasonic-assisted alcohol extraction of polyphenols from dandelion and their antioxidant activity[J]. Sci Technol Food Ind, 2017, 38(2):287-292.DOI: 10.13386/j.issn1002-0306.2017.02.047.
[39]	曹小燕, 杨海涛. 响应面法优化超声辅助提取荠菜多酚工艺及其抗氧活性研究[J]. 食品工业科技, 2019, 40(2):223-228,232.
	CAO X Y, YANG H T. Optimization of ultrasonic assisted extraction technology of polyphenol by response surface methodology from Capsella bursa-pastoris and its antioxidant activity[J]. Sci Technol Food Ind, 2019, 40(2):223-228,232.DOI: 10.13386/j.issn1002-0306.2019.02.038.
[40]	裴斐, 陶虹伶, 蔡丽娟, 等. 响应面试验优化辣木叶多酚超声辅助提取工艺及其抗氧化活性[J]. 食品科学, 2016, 37(20):24-30. doi: 10.7506/spkx1002-6630-201620005
	PEI F, TAO H L, CAI L J, et al. Optimization of polyphenols from Moringa oleifera Lam.leaves by ultrasound-assisted extraction using response surface methodology and their antioxidant activities[J]. Food Sci, 2016, 37(20):24-30.DOI: 10.7506/spkx1002-6630-201620005.
[41]	彭小进, 王天淼, 孙墨珑. 核桃楸叶多酚提取工艺的响应面法优化及其抗氧化性分析[J]. 福建农业学报, 2017, 32(7):746-750.
	PENG X J, WANG T M, SUN M L. Process optimization and antioxidant activity of polyphenols extracted from leaves of Juglans mandshurica Maxim[J]. Fujian J Agric Sci, 2017, 32(7):746-750.DOI: 10.19303/j.issn.1008-0384.2017.07.011.
[42]	徐百昌, 方紫依, 蒙锦燕, 等. 杂交构树叶多酚提取工艺优化及其抗氧化活性研究[J]. 中国畜牧兽医, 2021, 48(3):1046-1053.
	XU B C, FANG Z Y, MENG J Y, et al. Optimization of extraction process and antioxidant activity of polyphenols from leaves of Broussonetia papyrifera[J]. China Animal Husb & Vet Med, 2021, 48(3):1046-1053.DOI: 10.16431/j.cnki.1671-7236.2021.03.029.
[43]	李媛, 施欢贤. 文冠果壳总多酚的含量测定[J]. 中国民族民间医药, 2017, 26(16):13-14.
	LI Y, SHI H X. Determination the total polyphenol content of Xanthoceras sorbifolia Bunge’s husk[J]. Chin J Ethnomedicine Ethnopharmacy, 2017, 26(16):13-14.
[44]	马飞跃, 耿炬, 乔健, 等. 不同品种桑葚叶总酚含量及其抗氧化活性比较[J]. 热带作物学报, 2021, 42(3):888-896.
	MA F Y, GENG J, QIAO J, et al. Comparison of total phenol content and antioxidant activity from different mulberry leaves[J]. Chin J Trop Crops, 2021, 42(3):888-896.DOI: 10.3969/j.issn.1000-2561.2021.03.039.
[45]	薛雯, 房玉林, 袁照程. 葡萄不同营养器官多酚物质含量及抗氧化性的季节性变化[J]. 西北农业学报, 2020, 29(11):1666-1676.
	XUE W, FANG Y L, YUAN Z C. Seasonal changes of phenolic contents and antioxidant activity in different nutritive organs of grape[J]. Acta Agric Boreali Occidentalis Sin, 2020, 29(11):1666-1676.DOI: 10.7606/j.issn.1004-1389.2020.11.008.
[46]	李晓英, 薛梅, 樊汶樵. 蓝莓花、茎、叶酚类物质含量及抗氧化活性比较[J]. 食品科学, 2017, 38(3):142-147. doi: 10.7506/spkx1002-6630-201703024
	LI X Y, XUE M, FAN W Q. Comparison of antioxidant activity and phenolic contents of blueberry flowers,stems and leaves[J]. Food Sci, 2017, 38(3):142-147.DOI: 10.7506/spkx1002-6630-201703024.
[47]	林宝妹, 邱珊莲, 张少平, 等. 不同品种嘉宝果叶片提取物的抗氧化活性[J]. 西北农林科技大学学报(自然科学版), 2018, 46(9):121-130.
	LIN B M, QIU S L, ZHANG S P, et al. Antioxidant capacities of different leaf extracts of Jaboticaba cultivars[J]. J Northwest A & F Univ (Nat Sci Ed), 2018, 46(9):121-130.DOI: 10.13207/j.cnki.jnwafu.2018.09.016.

Metrics

Comments

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

方差来源 source	平方和 sum of squares	自由度 df	均方差 mean square	F	P
回归模型 model	196.360	14	14.030	39.920	<0.000 1^**
乙醇体积分数(A) ethanol volume fraction	19.530	1	19.530	55.590	<0.000 1^**
料液比(B) material liquid ratio	0.145	1	0.145	0.413	0.530 7
提取时间(C) extraction time	12.080	1	12.080	34.380	<0.000 1^**
提取温度(D) extraction temperature	123.070	1	123.070	350.250	<0.000 1^**
AB	0.055	1	0.055	0.157	0.697 8
AC	1.010	1	1.010	2.870	0.112 1
AD	4.860	1	4.860	13.840	0.002 3^**
BC	0.476	1	0.476	1.350	0.263 9
BD	0.416	1	0.416	1.180	0.294 9
CD	0.081	1	0.081	0.231	0.638 1
A²	20.470	1	20.470	58.260	<0.000 1^**
B²	4.520	1	4.520	12.860	0.003 0^**
C²	1.610	1	1.610	4.570	0.050 6
D²	2.080	1	2.080	5.910	0.029 0^*

指标 index	抗氧化能力指数/% antioxidant capacity index
指标 index	Ql	Ny	Lc	Ib	Ic	Lj	Xk	Hq	Iw	Sy	Jc	Af	Gz	Hz	Xc
自由基清除率 free radical scavenging rate	99.46	100.00	98.82	93.87	94.01	93.87	90.22	78.51	86.75	86.53	81.04	78.10	84.00	69.43	77.93
总抗氧化能力 total antioxidant capacity	100.00	90.85	86.27	88.89	73.86	66.01	62.09	70.59	60.78	58.82	64.05	63.40	46.41	59.48	47.71
APC	99.73	95.42	92.55	91.38	83.93	79.94	76.16	74.55	73.77	72.68	72.54	70.75	65.20	64.46	62.82
排序 order	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15

指标 index	来源 origin	多酚含量 polyphenol content	DPPH 清除能力 DPPH removal capacity	总抗氧化能力 total antioxidant capacity
总多酚含量 total polyphenol content	0.622^**	1
DPPH清除能力 DPPH removal capacity	0.763^**	0.825^**	1
总抗氧化能力 total antioxidant capacity	0.526^**	0.510^**	0.619^**	1

Extraction technology and antioxidant activity analyses of total polyphenols from Xanthoceras sorbifolia leaves

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 7

References 47

Related Articles 15

Recommended Articles

Metrics

Comments

Author

Reader

Reviewer

序号 No.	乙醇体积分数/% ethanol volume fraction	料液比/ (g·mL^-1) solid- liquid ratio	提取时间/min extraction time	提取温度/℃ extraction temperature	总多酚含量/ (mg·g^-1) totalpoly- phenol content
1	40	1∶20	30	60	35.81
2	50	1∶20	30	60	37.68
3	60	1∶20	30	60	39.17
4	70	1∶20	30	60	40.27
5	80	1∶20	30	60	34.85
6	90	1∶20	30	60	30.05
7	60	1∶10	30	60	35.52
8	60	1∶15	30	60	38.18
9	60	1∶20	30	60	39.41
10	60	1∶25	30	60	39.60
11	60	1∶30	30	60	42.86
12	60	1∶35	30	60	40.50
13	60	1∶20	20	60	34.51
14	60	1∶20	30	60	38.54
15	60	1∶20	40	60	41.86
16	60	1∶20	50	60	40.61
17	60	1∶20	60	60	44.16
18	60	1∶20	70	60	45.54
19	60	1∶20	30	30	22.32
20	60	1∶20	30	40	27.31
21	60	1∶20	30	50	30.69
22	60	1∶20	30	60	40.24
23	60	1∶20	30	70	38.18
24	60	1∶20	30	80	44.50

序号 No.	乙醇体积分数/% ethanol volume fraction	料液比/ (g·mL^-1) liquid ratio	提取时间/min extraction time	提取温度/℃ extraction temperature	总多酚含量/ (mg·g^-1) total polyphenols content
1	50	1∶20	40	60	39.65
2	70	1∶20	40	60	37.69
3	50	1∶30	40	60	39.82
4	70	1∶30	40	60	37.39
5	60	1∶25	30	50	34.85
6	60	1∶25	50	50	37.92
7	60	1∶25	30	70	41.66
8	60	1∶25	50	70	44.16
9	50	1∶25	40	50	34.97
10	70	1∶25	40	50	34.32
11	50	1∶25	40	70	43.66
12	70	1∶25	40	70	38.60
13	60	1∶20	30	60	39.50
14	60	1∶30	30	60	38.64
15	60	1∶20	50	60	39.54
16	60	1∶30	50	60	40.06
17	50	1∶25	30	60	36.64
18	70	1∶25	30	60	35.04
19	50	1∶25	50	60	40.15
20	70	1∶25	50	60	36.54
21	60	1∶20	40	50	37.54
22	60	1∶30	40	50	37.79
23	60	1∶20	40	70	43.10
24	60	1∶30	40	70	44.64
25	60	1∶25	40	60	38.86
26	60	1∶25	40	60	39.33
27	60	1∶25	40	60	39.33
28	60	1∶25	40	60	39.89
29	60	1∶25	40	60	39.33

[1]	SHI Huimin, YE Jianren, WANG Yan, LU Lanxiang, SHI Jiwu. Optimizing spore-producing medium and culture conditions of Bacillus velezensis strain YH-18 by response surface methodology [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(1): 209-218.
[2]	CHEN Bolin, WANG Guibin, GUO Qirong, WANG Jiahong, SU Erzheng, CAO Fuliang. Optimization of enzymatic hydrolysis and fermentation conditions of Ginkgo biloba wine using response surface methodology [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(5): 135-142.
[3]	ZHAO Yinghui, YANG Haicheng, ZHEN Zhen. Tree height estimations for different forest canopies in natural secondary forests based on ULS, TLS and ultrasonic altimeter systems [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(4): 23-32.
[4]	LIN Yuan, CHEN Pei, ZHOU Mingming, SHANG Xulan, FANG Shengzuo. Key bioactive substances and their antioxidant activities in Cyclocarya paliurus (Batal.) Iljinskaja leaves collected from natural populations [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(2): 10-16.
[5]	ZENG Jun, SUN Huizhen. Classification of ultrasonic acoustic emissions features on determining embolism-related signals [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2018, 42(01): 89-97.
[6]	ZHANG Huihui, LIU Longyun, WU Caie, LI Tingting, FAN Gongjian, YING Ruifeng. Semi-bionic extraction of flavonoids from Rosa roxburghii Tratt. [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2017, 41(06): 47-53.
[7]	XU Jingzhu, WU Caie, YING Ruifeng, LI Tingting, FAN Gongjian,FANG Shengzuo. Hypoglycemic and antioxidant activity of various polysaccharides from Cyclocarya paliurus leaves in vitro [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2017, 41(04): 6-12.
[8]	ZHOU Ying,LI Zicheng,SHEN Da,ZHAO Guomin,SHEN Weijie, ZHOU Xiaoyan,HAN Shuguang. Liquefaction conditions optimization of peanut shell by phenol using response surface analysis [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2015, 39(06): 137-142.
[9]	ZHANG Huihui, WU Caie, FAN Gongjian, YING Ruifeng, LI Tingting. Refining and antioxidant activity of flavonoids from Rosa roxburghii Tratt. [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2015, 39(03): 101-105.
[10]	HUANG Jianfeng, CHEN Nairong, LIN Qiaojia,RAO Jiuping,ZENG Qinzhi. Optimization of preparation of mid-temperature curing phenol-formaldehyde resin by response surface methodology [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2014, 38(03): 179-183.
[11]	HE Wen, YOU Jun, JIANG Shenxue, ZHANG Qisheng. Isolation and characterization analysis of cellulose nanocrystal from Moso bamboo [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2013, 37(04): 95-98.
[12]	DENG Bo， SHANG Xulan， FANG Shengzuo*， QIAN Chenyun. Optimization of ultrasonicassisted extraction of total triterpenoid compounds from Cyclocarya paliurus leaves [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2012, 36(06): 101-104.
[13]	SUN Jing，YOU Yi，JIANG Faxian，YANG Rui，CAI Junli，ZHAO Linguo*. Preparation and purification of extracellular polysaccharide from Coriolus versicolor [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2012, 36(06): 85-89.
[14]	JIANG Hua, ZHANG Yang, WANG Xuefei, MA Yuejin. Study on removal of lipophilic extractives from wheat straw by Candida cylindracea lipases [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2012, 36(02): 35-41.
[15]	YANG Hong, GE Jie, ZHANG Hui, PI Chengzhong. Study on removing silicate scale on disk disperser with the alkaline phonochemistry method [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2011, 35(04): 101-105.