
Assay for transposase-accessible chromatin with high-throughput sequencing and its application prospect in woody plants
WANG Ziyue, ZHEN Yan, LIU Guangxin, XI Mengli
JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (5) : 1-10.
Assay for transposase-accessible chromatin with high-throughput sequencing and its application prospect in woody plants
Assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) is an important technique established in 2013 in human immune cells to study epigenetic regulation. This technique has been used in the identification of genomic regulatory elements, binding sites of transcription factors and analysis of transcriptional regulation mechanism in human and mouse model animals. However, the application of ATAC-seq technique in plants is still in its infancy, and related studies mainly focus on model plants such as Arabidopsis and rice. In this review, we discuss the application of ATAC-seq in plants, including chromatin accessibility sites mapping, stress resistance mechanisms revealing, epigenetic modification and regulatory elements identification. Furthermore, we look at the prospects of applying ATAC-seq in woody plant epigenomics so as to promote its application in woody plant epigenomics research.
assay for transposase-accessible chromatin with high-throughput sequencing(ATAC-seq) / woody plants / chromatin accessibility / epigenomic
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