Progresses and achievements of genetic improvement on Masson pine (Pinus massoniana) in China

doi:10.12302/j.issn.1000-2006.202207020

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (6): 10-22.doi: 10.12302/j.issn.1000-2006.202207020

Special Issue: 南京林业大学120周年校庆特刊

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Progresses and achievements of genetic improvement on Masson pine (Pinus massoniana) in China

JI Kongshu(), XU Li’an, WANG Dengbao, NI Zhouxian, WANG Zhangrong

Key Laboratory of Forest Genetics & Biotechnology of the Ministry of Education, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China

Received:2022-07-11 Revised:2022-08-11 Online:2022-11-30 Published:2022-11-24

Abstract

Abstract:

The genetic improvement of Masson pine (Pinus massoniana) in China started in the 1950s, comprehensively developed in the late 1970s and early 1980s, and rapidly advanced with the integration of post-modern biotechnology in the new century. In this review, we comprehensively summarized the research work (including provenance trials, superior tree selection, establishment of seed production stand and seed orchard, clone selection, wood properties improvement, and modern biotechnology-assisted breeding), major achievements, innovation, and leading role and contribution to society of the Nanjing Forestry University Group on Masson pine over the past 60 years. Additionally, the improvement experience is summarized. Facing new opportunities and challenges, and in view of the characteristics of the species, existing research basis and social needs, and development trend in the field of biology, we suggest that the genetic improvement of Masson pine in the future should be based on the work of germplasm resources, multi-objective selection and breeding, family and clone forestry needs, and integration of conventional and modern biotechnology. More attention should be given to the breeding fields of pine wood nematode-resistant, pulp making, and high-yield turpentine varieties, and basic and advanced research and its integration into conventional breeding. The above research will be beneficial to the genetic improvement of this tree species.

Key words: Masson pine(Pinus massoniana), genetic improvement, research review and prospect, achievement, strategic response

CLC Number:

S722

JI Kongshu, XU Li’an, WANG Dengbao, NI Zhouxian, WANG Zhangrong. Progresses and achievements of genetic improvement on Masson pine (Pinus massoniana) in China[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(6): 10-22.

Figures/Tables 6

Table 1

Table 2

Fig.1

Table 3

Table 4

The genetic gain of progeny test of Pinus massoniana in Fujian Province"

测定林材料 test material	地点site	林龄/a age	性状 characteristic	家系/个 familie	对照 CK	增益 gain	参考文献 source of literature
马尾松优树自由授粉子代林	6个林场的多点试验	7	树高	598	天然林分种子	高于CK的10%家系293个,占49%家系。家系遗传力( $h f 2$ )为31%,单株遗传力( $h i 2$ )为24%,设选择强度2.67,建立无性系或实生苖种子园增益分别为12%~13%	[17]
马尾松种子园自由授粉子代测定林	漳平五一国有林场	8	材积	120	天然林分种子	55.5%家系>CK,高于CK 10% 以上的家系占 22.7%。入选率30%,建立无性系种子园遗传增益为12.33%	[18]
马尾松种子园自由授粉子代测定林	三明市官庄国有林场	10	材积	233	天然林分种子	高于CK 10%的家系占 22.7%,入选70个家系建立无性系种子园,增益28.51%	[19]
漳平五一林场种子园的36家系种子	将乐国有林场	5	材积	36	当地林分种子	子代群体>CK,增益11.37%	[20]
马尾松种子园自由授粉子代测定林	漳平五一国有林场	12	材积	162	当地母树林种子	子代均值>CK的达30.5%,其中47个家系与对照差异极显著,占27%。从优良家系中选择优株,建立第2代种子园,预估遗传增益46%	[21]
实生种子园子代	上杭县白砂国有林场	5、6	材积	70	当地人工林种子	子代测定的现实遗传增益8.54%。实生种子园进行家系15%、家系内85%的去劣疏伐,遗传增益预期再提高5.70%	[22]

Table 4

Table 5

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时期 stage	项目(课题/专题)名称 project name	主持单位 the affiliations in charge
“七五”	马尾松种子园建立技术的研究	南京林业大学,中国林业科学研究院亚热带林业研究所
“八五”	短周期工业用材林良种选育马尾松专题	南京林业大学,中国林业科学研究院亚热带林业研究所
“九五”	马尾松纸浆材树种良种选育及培育技术研究	贵州大学,南京林业大学,中国林业科学研究院亚热带林业研究所
“十五”	马尾松纸浆材二代育种和持续高产经营技术研究	南京林业大学,中国林业科学研究院亚热带林业研究所
“十一五”	高产优质多抗马尾松新品种选育	中国林业科学研究院亚热带林业研究所,南京林业大学
“十二五”	马尾松二代育种技术研究与示范	中国林业科学研究院亚热带林业研究所,南京林业大学

序号 No.	成果名称 name of achievements	年份 year	奖项 class of the award	排名 ranking
1	马尾松第1代无性系种子园建立技术	1993	国家科技进步三等奖	第一
2	马尾松材性遗传变异与制浆造纸材优良种源选择	1999	国家科技进步二等奖	第一
3	主要针叶纸浆用材树种新品种选育、规模化繁殖及培育配套技术	2002	国家科技进步二等奖	第四
4	马尾松良种选育及高产高效配套培育技术研究及应用	2009	国家科技进步二等奖	第三

地点 site	建成年份 year	建园材料 materials	面积/hm² area	世代 generation	种子园类型 type	现状 present situation
沙县官庄国有林场	1984定砧,1985嫁接	106个无性系	11.33	1	无性系	已改建为种质资源库
	1989	198个无性系	8.80	1	无性系	已改建为种质资源库
	2008	80个无性系	8.00	2		正常产种
漳平市五一国有林场	1985定砧,1986—1987嫁接;1992	218个无性系	22.07	1	无性系	1997、2001、2005年分3次去劣疏伐,最后保存无性系150~180株/ hm²,转化成去劣种子园。目前已改建为种质资源库
	2009	90个无性系	18.73	2	无性系	2020年底疏伐,现保留密度300~330株/hm²。正常产种
	2019	30个无性系	2.00	2	无性系	正常产种
上杭县白砂国有林场	1991	56个家系	7.67	1	实生苗	2000、2004年分别疏伐。已改建为种质资源库
	1992	142个优良家系	4.67	1	实生苗	为种子生产与优树子代测定为一体的种子园。目前为测定林
	2009	58个无性系	13.33	2	无性系	正常产种
华安县西陂国有林场	2010、2012	42个无性系	24.20	2	无性系	已改建为种质资源库

序号 No.	克隆的基因名称 name of cloned gene	基因主要功能 main function of the gene	参考文献 source of the literature
1	PmCOMT、PmCAD、PmCesA1、PmCesA2、PmMYB7、PmMYB4、 PmAP2/ERF、PmbHLH、PmWRKY	与木材形成相关,与纤维素、木质素、次生壁的合成相关	[63⇓⇓⇓⇓⇓-69]
2	PmDXR、PmGPPS、PmDXS1、PmDXS2、PmDXS3、PmMCT、PmCMK、 PmMDS、PmHDS、 PmHDR1、PmHDR2、 PmIPPI、PmCYP720B1	参与马尾松萜类物质的生物合成和转录调控,与产脂、抗线虫相关	[70⇓⇓⇓⇓⇓⇓-77]
3	PmPIN1、PmPIN2、PmIAA1、PmIAA2、PmIAA3、PmTIR1	与生长素运输和信号转导相关	[78⇓⇓-81]
4	PmCYP734A	与细胞分裂素合成相关	[82]
5	PmRCA1、PmRCA2、PmPGK1、PmGPIC	与光合作用相关	[83⇓-85]
6	Pm LFY、Pm NLY、Pm TFL1、PmMADS13	与成花相关	[86⇓-88]
7	Pm SHR、Pm SCR、Pm SCL1	与根发育相关	[89]
8	PmNAC8、PmAOX、PmSnRK2s、PmSnRK2.3	与抗非生物胁迫相关	[90⇓⇓⇓-94]
9	miRNAs	与球果分化与发育、木质部发育相关	[95-96]

Progresses and achievements of genetic improvement on Masson pine (Pinus massoniana) in China

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