Characterization of IpSAD gene family in Idesia polycarpa and functional identification

GONG Jue, BI Hao, YANG Wenlu, WANG Dongming, WANG Lehui, JIANG Yuanzhong, MA Tao

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (6) : 261-270.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (6) : 261-270. DOI: 10.12302/j.issn.1000-2006.202207034

Characterization of IpSAD gene family in Idesia polycarpa and functional identification

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Abstract

【Objective】Stearoyl-ACP Δ9 desaturase (SAD) is a key enzyme that determines the composition of fatty acids. The whole-genome identification of Idesia polycarpa SAD genes can provide targets to improve fatty acid compositions by genetic methods.【Method】HMM and Blastp were used to identify IpSAD genes in the I. polycarpa genome. The gene structures, phylogenetic relationship, conserved motifs, physicochemical characters of IpSADs, and cis-regulatory element in these gene promoters were analyzed and predicted by MEGA X, MEME and PlantCare online software, respectively. The MCSCANX software was used to reveal the common linear relationship of SAD genes in I. polycarpa and P. trichocarpa. The tissue expression pattern of IpSAD genes was constructed according to RNA-seq, and the function of these genes was identified by a virus-induced gene silencing (VIGS) system.【Result】(1)A total of seven IpSAD genes (IpSAD1-7) were identified in the I. polycarpa genome, and these family members shared high sequence similarity and conserved domains. (2)Aphylogenetic tree indicated that IpSAD members were divided into three clades, which were consistent with the classification of the homologs in Arabidopsis thaliana. In particular,IpSAD2, IpSAD3 and IpSAD4 were closer to AtSSI2, which is the key enzyme of oleic acid biosynthesis. (3)The cis-regulatory elements in the promoters of IpSAD genes included elements of light and abscisic acid responses. (4)These IpSAD genes were divided into three categories according to their tissue expression patterns. The IpSAD genes in Classes Ⅰ and Ⅱ were expressed with a low level in most tissues, whereas Class Ⅲ genes showed a high level in all tested tissues. (5) Silencing IpSAD genes using VIGS significantly reduced the oleic acid content of leaves.Remarkably, the oleic acid content in IpSAD3 silenced leaves was decreased by up to 76%.【Conclusion】The characteristics of each IpSAD gene was clarified, and their functions in oleic acid synthesis were determined. Our findings can aid in the investigation of the biosynthesis mechanisms of oleic acid and total oil in I. polycarpa.

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Idesia polycarpa / stearoyl-acyl carrier protein Δ9 desaturase (SAD) / oleic acid / virus induced gene silencing(VIGS)

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GONG Jue , BI Hao , YANG Wenlu , et al . Characterization of IpSAD gene family in Idesia polycarpa and functional identification[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2022, 46(6): 261-270 https://doi.org/10.12302/j.issn.1000-2006.202207034

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