Screening and analysis of differential secondary metabolites in Castanea mollissima with different levels of resistance to Oligonychus ununguis

doi:10.12302/j.issn.1000-2006.202209053

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2024, Vol. 48 ›› Issue (2): 234-240.doi: 10.12302/j.issn.1000-2006.202209053

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Screening and analysis of differential secondary metabolites in Castanea mollissima with different levels of resistance to Oligonychus ununguis

ZHANG Xinfang(), WANG Guangpeng^*(), ZHANG Shuhang, LI Ying, GUO Yan

Changli Institute of Pomology, Hebei Academy of Agricultural and Forestry Sciences, Changli 066600, China

Received:2022-09-25 Revised:2023-03-24 Online:2024-03-30 Published:2024-04-08

Abstract

Abstract:

【Objective】 The differential secondary metabolites in chestnut (Castanea mollissima) leaves with different mite resistance levels were screened to provide a reference for the analysis of the mite(Oligonychus ununguis) resistance mechanism and the breeding of highly mite-resistant chestnut varieties.【Method】 The chestnut varieties ‘Yanxing’ and ‘Likang’ were used to identify and determine the mite resistance levels to O. ununguis with the field investigation method. Metabolomics detection was performed using ultra-high-performance liquid chromatography and tandem mass spectrometry, and the differential metabolites were screened using a combination of fold change and the variable importance in projection value. The R software (ComplexHeatmap and MetaboAnalystR package) was used to draw the clustering heat map and orthogonal partial least squares-discriminant analysis score map. Identified metabolites were annotated using the Kyoto encyclopedia of genes and genomes (KEGG) database, and the annotation results were classified by pathway types.【Result】 The resistance levels of ‘Likang’ and ‘Yanxing’ to O. ununguis were high resistance and susceptible, respectively. A total of 704 secondary metabolites were detected, including 165 differential metabolites. The content of 73 metabolites in ‘Likang’ was significantly higher than in ‘Yanxing’, and the content of 92 metabolites was lower in ‘Likang’ than in ‘Yanxing’. The types of differential metabolites included 56 phenolic acids, 60 flavonoids, 19 lignins and coumarins, four tannins, five alkaloids, 16 terpenoids and five other types. Flavonoids and phenolic acids accounted for 36% and 34%, respectively. Metabolites present only in ‘Likang’ included caffeoylcholine-4-O-glucoside, 3-hydroxy-5-methylphenol-1-O-(6'-galloyl)glucoside, tricin-7-O-glucuronide, 6'-trans-cinnamoyl-8-epikingisidic acid, lariciresinol-4'-O-glucoside, albanol B and sanguiin H11. There were 15 metabolites present only in ‘Yanxing’, including 4-methyl-5-thiazoleethanol, 2,4-dihydroxybenzoic acid, isofraxidin, salicin and roxburic acid. Thirty-three differential metabolites in ‘Yanxing’ and ‘Likang’ were annotated to 12 metabolic pathways.【Conclusion】 The secondary metabolic profiles between the mite-susceptible variety ‘Yanxing’ and the highly resistant variety ‘Likang’ differed. Secondary metabolites that may be related to chestnut mite resistance were prunetin, epigallocatechin, caffeic acid, ferulic acid, several lignans and 4-methyl-5-thiazoleethanol. The differential secondary metabolites were mainly annotated and enriched in the flavonoid biosynthesis pathway and the flavone and flavonol biosynthesis pathway.

Key words: Castanea mollissima, Oligonychus ununguis, mite resistance, secondary metabolites

CLC Number:

S718
S763.3

ZHANG Xinfang, WANG Guangpeng, ZHANG Shuhang, LI Ying, GUO Yan. Screening and analysis of differential secondary metabolites in Castanea mollissima with different levels of resistance to Oligonychus ununguis[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(2): 234-240.

Figures/Tables 5

Table 1

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等级level	螨害情况mite condition
0	叶片不失绿或几乎不失绿leaf blade is almost green
1	叶片失绿面积占叶片总面积的25%以下leaf chlorosis area accounts for less than 25% of total leaf area
2	叶片失绿面积占叶片总面积的26%~50% leaf chlorosis area accounts for 26%-50% of total leaf area
3	叶片失绿面积占叶片总面积的51%~75% leaf chlorosis area accounts for 51%-75% of total leaf area
4	叶片失绿面积占叶片总面积的76%~100% leaf chlorosis area accounts for 76%-100% of total leaf area

Screening and analysis of differential secondary metabolites in Castanea mollissima with different levels of resistance to Oligonychus ununguis

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