
A low-field nuclear magnetic resonance detection of moisture changes in different water phases during the imbibition and stratification process of Cercis chinensis seeds
GONG Nan, ZU Xin, XIE Zhijun, ZHU Changhong, LI Shuxian
JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2023, Vol. 47 ›› Issue (6) : 42-50.
A low-field nuclear magnetic resonance detection of moisture changes in different water phases during the imbibition and stratification process of Cercis chinensis seeds
【Objective】Using low-field nuclear magnetic resonance (NMR) technology, changes in the water phase during the imbibition and stratification of Cercis chinensis seeds were detected nondestructively. Combined with the nutrient content changes during stratification, the role of each water phase was analyzed to reveal the material base and physiological conditions during the germination of C. chinensis seeds. 【Method】After being treated with hot water at 80 ℃, the hardness of C. chinensis seeds was removed and its water absorption curve was calculated by the gravimetric method. Cold stratification was applied to break the physiological dormancy of C. chinensis seeds by random selection. The germination percentage and nutrient content of seeds were determined during different stratification periods. Low-field NMR was employed to investigate the dynamic transitions of the water phase and water content during imbibition and stratification. 【Result】(1)Hot water immersion at 80 ℃ for 5 min followed by 60 days of cold stratification effectively improved the germination rate of C. chinensis seeds. (2)The water absorption curve of C. chinensis seeds exhibited an “S” type variation and from 0 h to 9 h was the rapid water absorption stage. At from 9 h to 24 h, seed water absorption gradually slowed. Ultimately, the seeds entered a balanced water absorption stage after 24 h. (3)NMR spectra revealed that the water mass (x) of C. chinensis seeds and the peak areas of the NMR relaxation spectra (y) exhibited a significant linear relationship, according to the linear regression equation y = 164 604.7 x + 4 962.3, with a correlation coefficient R2 = 0.999 6. (4)NMR T2 relaxation spectra enabled division of the water phases during the imbibition process of C. chinensis seeds into three types: bound water T2a, free water T2b, and newly absorbed water T2c. (5)During the water absorption process from 0 to 3 h, the content of T2a decreased continuously, whereas T2b content increased dramatically. After 3 h, T2c appeared and T2a disappeared. Only T2b and T2c existed in the seeds; both showed an increasing trend, with the peak maximum moving to the right and water mobility being enhanced. Peaks T2b and T2c shifted to the right during stratification, with fluctuating changes in peak area and peak ratio, and in general showed a trend towards increased water mobility. (6)During the stratification process of C. chinensis seeds, the starch content continued to decrease, soluble sugar content increased, and soluble protein content showed an increasing trend, followed by a decreasing trend. 【Conclusion】Water mobility is enhanced during the absorption and the stratification of C. chinensis seeds. There are three types of water phases in the imbibition process of C. chinensis seeds: T2a, T2b and T2c. However, only T2b and T2c appear during stratification. The changes in the nutrient contents of starch, soluble sugars, and soluble proteins roughly follow the same trend as those in T2b content. The fluctuations in water in each phase provides a suitable water environment for C. chinensis seed germination, and the changes in T2b during the stratification process might be closely related to germination.
Cercis chinensis / imbibition / stratification / low-field nuclear magnetic resonance / water phase / nutritive materials
[1] |
胡晋. 种子生物学[M]. 北京: 高等教育出版社, 2006.
|
[2] |
宋平. 基于低场核磁共振技术的水稻浸种过程种子水分检测研究[D]. 沈阳: 沈阳农业大学, 2016.
|
[3] |
阮榕生. 核磁共振技术在食品和生物体系中的应用[M]. 北京: 中国轻工业出版社, 2009.
|
[4] |
|
[5] |
牟红梅, 何建强, 邢建军, 等. 小麦灌浆过程籽粒水分变化的核磁共振检测[J]. 农业工程学报, 2016, 32 (8): 98-104.
|
[6] |
付晓记, 唐爱清, 闵华, 等. 花生浸种过程中水分相态和水分迁移动态研究[J]. 中国油料作物学报, 2018, 40(4): 552-557.
|
[7] |
|
[8] |
|
[9] |
李进宇, 赵爽, 王茂良, 等. 紫荆属(Cercis L.)种质资源情况及研究进展[J]. 西北林学院学报, 2021, 36(6): 145-152.
|
[10] |
康四和, 邓海英. 湖北紫荆皮植物学及生药鉴定研究[J]. 时珍国医国药, 2010, 21(1): 185-187.
|
[11] |
王彦玲, 陆帅, 牛若琳. 紫荆的栽培技术及应用[J]. 陕西农业科学, 2010, 56(4): 233-234.
|
[12] |
周健. 紫荆种子休眠特性及其吸水机制研究[D]. 南京: 南京林业大学, 2016.
|
[13] |
宋平, 杨涛, 王成, 等. 利用低场核磁共振分析水稻种子浸泡过程中的水分变化[J]. 农业工程学报, 2015, 31(15): 279-284.
|
[14] |
李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000.
|
[15] |
刘潇, 沈飞, 黄怡, 等. 基于 LF-NMR 的糙米发芽过程水分状态变化[J]. 中国粮油学报, 2018, 33(4): 7-12.
|
[16] |
杜恬恬, 代松, 钱滕, 等. 基于核磁共振技术的合欢种子吸水特性[J]. 林业科学, 2022, 58(4): 22-31.
|
[17] |
袁鸣, 朱铭玮, 侯静, 等. 利用低场核磁共振技术检测刺槐种子吸水过程水分的变化[J]. 南京林业大学学报(自然科学版), 2022, 46(2): 135-142.
|
[18] |
|
[19] |
彭宇飞. 基于核磁共振技术玉米种子萌发过程水分分布与变化研究[D]. 沈阳: 沈阳农业大学, 2018.
|
[20] |
杨鹏, 陆兰芳, 王展, 等. 基于低场核磁共振技术监测谷子萌发过程中内部水分变化[J]. 食品工业科技, 2020, 41(14): 65-68, 74.
|
[21] |
宋平, 彭宇飞, 王桂红, 等. 玉米种子萌发过程内部水分流动规律的低场核磁共振检测[J]. 农业工程学报, 2018, 34 (10): 274-281.
|
[22] |
潘瑞炽. 植物生理学[M]. 7版. 北京: 高等教育出版社, 2012.
|
/
〈 |
|
〉 |