JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2024, Vol. 48 ›› Issue (2): 61-68.doi: 10.12302/j.issn.1000-2006.202302014
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SONG Ziqi1,2(), BIAN Guoliang1, LIN Feng3, HU Fengrong4, SHANG Xulan1,*(
)
Received:
2023-02-14
Revised:
2023-03-23
Online:
2024-03-30
Published:
2024-04-08
CLC Number:
SONG Ziqi, BIAN Guoliang, LIN Feng, HU Fengrong, SHANG Xulan. Establishment and application of a flow cytometry method for chromosome ploidy identification of Cyclocarya paliurus[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(2): 61-68.
Fig. 1
Testing effects of ploidy analysis of Cyclocarya paliurus with different nuclei isolation buffers 2x-CK indicates the diploidy standard sample; S indicates the tetriploidy test sample; CV indicates the coefficient of variation in S; The different letters indicate that the significant differences among treatments (P<0.05). The same below."
Fig. 2
Testing effect of ploidy analysis of C. paliurus with different centrifugal treatments L1 and L2 indicate that nuclei suspensions centrifuge at 1 000 r/min for 5 and 10 min, respectively; L3 and L4 indicate that nuclei suspensions centrifuge at 2 000 r/min for 5 and 10 min, respectively; NL indicates no centrifugal treatment."
Table 2
Test results of ploidy of 1 395 C. paliurus germplasm resources by FCM"
来源 resource | 数量 number of samples | 倍性 ploidy | 占比/% proportion | 变异系数/% CV |
---|---|---|---|---|
种质资源库 germplasm bank | 1 087 | 2 | 4.05 | 2.45~4.22 |
4 | 95.95 | 2.05~4.86 | ||
安徽省 Anhui Province | 75 | 2 | 38.67 | 3.02~5.54 |
4 | 61.33 | 2.76~5.21 | ||
湖北省 Hubei Province | 73 | 2 | 42.46 | 4.32~6.08 |
4 | 57.54 | 2.98~5.41 | ||
四川省 Sichuan Province | 32 | 4 | 100.00 | 3.42~5.03 |
江西省 Jiangxi Province | 80 | 4 | 100.00 | 3.10~5.41 |
广西壮族自治区 Guangxi Zhuang Autonomous Region | 48 | 4 | 100.00 | 2.68~4.97 |
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