The relationship of appearance, structure and photosynthetic characteristics of different leaf color phenotypes of Sasaella glabra ‘Albostriata’

doi:10.12302/j.issn.1000-2006.202302035

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2025, Vol. 49 ›› Issue (1): 145-154.doi: 10.12302/j.issn.1000-2006.202302035

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The relationship of appearance, structure and photosynthetic characteristics of different leaf color phenotypes of Sasaella glabra ‘Albostriata’

XU Xinlu¹^,²(), KONG Shuxin¹^,², LYU Zhuo¹^,², JIANG Shuaijun¹^,², ZHAO Wanqi¹^,², LIN Shuyan¹^,^*()

1. Co-Innovation Center for Sustainable Forestry in Southern China,Bamboo Research Institute,Nanjing Forestry University, Nanjing 210037,China
2. College of Eolology and the Environment,Nanjing Forestry University,Nanjing 210037,China

Received:2023-02-28 Revised:2023-05-17 Online:2025-01-30 Published:2025-01-21
Contact: LIN Shuyan E-mail:936728807@qq.com;lrx@njfu.com.cn

Abstract

Abstract:

【Objective】We have used Sasaella glabra as a model to investigate the relationship between various leaf color phenotypes of S. glabra ‘Albostriata’ and their photosynthetic capabilities.【Method】Four different leaf color phenotypes were selected for the study. Image J software was used to measure the length, breadth, and surface area of the leaves. The dry weight of the leaves was determined using an electronic balance. Leaf color parameters were assessed using the color picker feature in Adobe Photoshop CC 2018. Photosynthetic pigment levels were measured using visible light spectrophotometry. Diurnal variations in photosynthesis and light response curves were recorded with the Li-6400 portable photosynthesis measurement system. Chlorophyll fluorescence parameters were measured using the CF Imager program. The relationships between leaf color parameters and photosynthetic pigments, as well as between photosynthetic pigments and the net photosynthetic rate, were analyzed. Chlorophyll and chlorophyll fluorescence parameters were also examined. The fluorescence microstructure and ultrastructure of leaves were observed according to leaf color division. 【Result】The biological characteristics of leaves with different color phenotypes varied significantly. For instance, S. glabra exhibited significantly higher leaf length, width, area, and dry weight compared to S. glabra ‘Albostriata’, with these indices increasing as the green stripe area expanded. Similarly, the leaf color parameters of different phenotypes were significantly different. The absolute value of the a^* value, indicating red-green coloration, increased, while the trends of the L^* value (brightness), b^* value (yellow-green coloration), and C^* value (chroma) all decreased synchronously. The microstructures of various leaf types showed no significant differences in cell organization. The primary distinction was that in S. glabra and the green area of S. glabra ‘Albostriata’, mesophyll cells differentiate into arm cells and rosette cells, respectively, with chloroplasts occupying the mesophyll cells. In contrast, mesophyll cells in the yellow area of S. glabra ‘Albostriata’ showed notable differences, such as thicker cell walls, larger cell cavities and more degradation products. The mesophyll cell shape and differentiation in the green area resembled those of S. glabra, except for the presence of osmiophilic granules. The four genotypes exhibited significant differences in chlorophyll a, b, total chlorophyll, and carotenoids levels. The S. glabra had the highest concentration of photosynthetic pigments. The amount of photosynthetic pigments increased with the stripe area, showing a significant positive correlation with the a^* value and a significant negative correlation with the L^*, b^*, and C^* values. The leaf color genotypes also differed in light response curves and diurnal photosynthesis fluctuations. Chlorophyll content was positively correlated with the net photosynthetic rate, stomatal conductance, and transpiration rate, and was directly proportional to F₀. The only significant variation in chlorophyll fluorescence parameters was F₀, indicating normal PS Ⅱ photoreaction center activity. 【Conclusion】 The three distinct leaf color phenotypes of S. glabra ‘Albostriata’ differ significantly in appearance, structure, and photosynthetic properties. The leaf color directly reflects these differences, illustrating that the number and development of chloroplasts affect chlorophyll content. Chlorophyll content is positively related to photosynthetic capacity. Morphological indicators such as leaf area and dry matter content can indicate the content of photosynthetic products, though the complex regulatory mechanisms behind this relationship warrant further investigation.

Key words: Sasaella glabra ‘Albostriata’, chlorophyll, chloroplast, leaf color parameter, photosynthetic characteristic

CLC Number:

S718.3

XU Xinlu, KONG Shuxin, LYU Zhuo, JIANG Shuaijun, ZHAO Wanqi, LIN Shuyan. The relationship of appearance, structure and photosynthetic characteristics of different leaf color phenotypes of Sasaella glabra ‘Albostriata’[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2025, 49(1): 145-154.

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叶色表型 leaf color phenotype	叶长/cm leaf length	叶宽/cm leaf width	叶面积/cm² leaf area	叶干质量/g leaf dry mater content	L^*	a^*	b^*
Y1	3.67±0.76 c	0.64±0.21 b	1.77±0.87 c	0.03±0.01 c	79.10±15.55 a	-3.50±3.69 a	44.30±8.81 a
Y2	4.93±0.77 b	0.73±0.08 b	2.64±0.61 bc	0.05±0.01 bc	69.80±24.18 ab	-7.10±5.63 ab	35.90±10.86 b
Y3	5.14±0.74 b	0.80±0.10 b	3.01±0.51 b	0.08±0.03 b	52.90±27.45 bc	-11.20±6.63 bc	27.10±9.69 c
CK	6.05±0.97 a	1.14±0.21 a	5.21±1.48 a	0.12±0.06 a	40.60±8.24 d	-14.30±2.71 d	19.60±6.45 c

叶色表型 leaf color phenotype	叶绿素a Chl a	叶绿素b Chl b	叶绿素 Chl	类胡萝卜素 Car
Y1	4.05±0.18 d	0.77±0.26 d	4.82±0.33 d	1.60±0.10 cd
Y2	11.85±0.07 c	2.43±0.19 c	14.28±0.26 c	3.31±0.05 bc
Y3	15.87±1.29 b	3.09±0.37 b	18.96±1.64 b	4.97±0.49 b
CK	28.69±0.54 a	5.65±0.40 a	34.34±0.45 a	8.15±0.52 a

指标 index	Chl a	Chl b	Chl	Car
L^*	-0.656^*	-0.688^*	-0.662^*	-0.674^*
a^*	-0.685^*	-0.693^*	-0.687^*	-0.726^**
b^*	-0.839^**	-0.849^**	-0.841^**	-0.844^**
C^*	-0.887^**	-0.888^**	-0.889^**	-0.889^**

叶色表型 leaf color phenotype	F₀	原初光能转化效率 F_v/F_m	光合电子传递量子效率 ΦPSⅡ	非光化学猝灭系数 qN	光化学猝灭系数 qP
Y1	2 449.67±694.09 bc	0.81±0.01 ab	0.15±0.02 a	3.67±0.78 a	0.31±0.08 a
Y2	3 233.00±75.34 ab	0.78±0.02 b	0.17±0.01 a	2.73±0.18 bc	0.31±0.02 a
Y3	3 335.67±356.85 ab	0.79±0.01 ab	0.16±0.01 a	3.14±0.08 abc	0.30±0.02 a
CK	3 580.00±595.05 a	0.80±0.01 ab	0.16±0.01 a	3.48±0.39 ab	0.32±0.01 a

指标index	Chl	Pn	Gs	Ci	Tr	F₀	F_v/F_m	ΦPSⅡ	qN
Pn	0.611^*	1
Gs	0.907^**	0.359	1
Ci	-0.060	-0.382	-0.011	1
Tr	0.816^**	0.266	0.825^**	0.122	1
F₀	0.656^*	0.429	0.770^**	-0.267	0.434	1
F_v/F_m	0.064	-0.215	-0.026	0.687^*	0.154	-0.509	1
ΦPSⅡ	0.025	-0.151	0.050	-0.116	0.054	-0.306	0.067	1
qN	0.017	-0.277	-0.165	0.598^*	0.156	-0.538	0.741^**	0.058	1
qP	0.085	-0.175	-0.022	0.058	0.282	-0.368	0.184	0.635^*	0.552

The relationship of appearance, structure and photosynthetic characteristics of different leaf color phenotypes of Sasaella glabra ‘Albostriata’

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