A comparative study on leaf characters between diploid and tetraploid of Cyclocarya paliurus

LIU Xialan, SONG Ziqi, HU Fengrong, SHANG Xulan

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2024, Vol. 48 ›› Issue (4) : 76-84.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2024, Vol. 48 ›› Issue (4) : 76-84. DOI: 10.12302/j.issn.1000-2006.202305024

A comparative study on leaf characters between diploid and tetraploid of Cyclocarya paliurus

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Abstract

【Objective】To compare the differences in leaf phenotypes characters, anatomical structures and stomatal characteristics between diploid and tetraploid Cyclocarya paliurus, we obtained data on the leaf characteristics of C. paliurus with different ploidy.【Method】Diploid and tetraploid seedlings of C. paliurus were used as study materials, and 32 leaf traits were measured. Independent samples t-tests, a correlation analysis, and principal component analysis (PCA) were conducted.【Result】There were no significant differences between diploid and tetraploid seedlings for sawtooth width and the position of the maximum leaf width of the middle leaflet. For the other 30 leaf traits, there were significant or extremely significant differences between diploid and tetraploid seedlings. Among them, the number of leaflets of tetraploids was significantly less than that of diploids, while the area and specific leaf weight of compound leaves of tetraploids were significantly greater than those of diploids. The length, width and area of the terminal and middle leaflets of tetraploids were significantly greater than those of diploids, while the leaf shape index was significantly smaller than that of diploids. The sawtooth density of tetraploids was significantly smaller than that of diploids. The leaflet thickness, the upper epidermis thickness, lower epidermis thickness, palisade tissue thickness, and sponge tissue thickness of tetraploids were all significantly higher than those of diploids, while the ratio of palisade tissue to sponge tissue and cell tense ratio were significantly smaller than those of diploids. The stomatal length and width of tetraploids were significantly greater than those of diploids, while the stomatal density was significantly smaller than that of diploids. The correlation analysis results indicated that there were broad and significant correlations between the phenotypic characteristics, anatomical structure, and stomatal characteristics of C. paliurus. The indicators reflecting the differences of leaf characteristics between diploid and tetraploid C. paliurus were extracted by a PCA, including leaflet width, leaflet area, leaf shape index, sponge tissue thickness, stomatal length, palisade tissue thickness and cell tense ratio.【Conclusion】There were significant differences in leaf traits between diploid and tetraploid C. paliurus, and leaf phenotypic characteristics, such as leaflet width, leaflet area, and leaf shape index, can be used for the preliminary screening of diploid and tetraploid C. paliurus.

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Cyclocarya paliurus / polyploid / phenotypic characters / anatomical structure / stomatal characteristics

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LIU Xialan , SONG Ziqi , HU Fengrong , et al. A comparative study on leaf characters between diploid and tetraploid of Cyclocarya paliurus[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2024, 48(4): 76-84 https://doi.org/10.12302/j.issn.1000-2006.202305024

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