【Objective】Liriodendron hybrids (L. ×sinoamericanum) have excellent characteristics beyond their parents. Currently, little is known about their sexual reproduction and seed abortion mechanisms, and there are few reports on the meiosis process and chromosome behavior of their pollen mother cells. This study provides a detailed observation of the chromosomal behavior of meiosis in pollen mother cells of L. × sinoamericanum hybrids. The results can provide important cytological basis for aneuploid breeding, hybrid breeding, and germplasm resource protection of Liriodendron.【Method】The collected anthers were squashed and stained with Carbol fuchsin before microscope study. Photographs of freshly prepared slides were taken with the automatic camera of Nikon Eclipse 50i microscope. 【Result】(1) Pollen mother cells of L. × sinoamericanum can undergo normal meiosis, 80% of which can complete the meiotic process, and produce normal pollen; (2) meiosis of pollen mother cells is asynchronous. The pollen mother cells in different parts of anthers above and below the same receptacles are in different stages, and meiotic asynchrony also exists within the same pollen mother cells. (3) 20% of pollen mother cells have obvious chromosomal abnormalities in the process of meiosis, including laggard chromosomes, prematurely separated chromosomes, and micronuclei; (4) there is a diversity of spindle behavior during meiosis, with a variety of spindles, such as parallel spindles, vertical spindles and 60° or 120° spindles. 【Conclusion】Despite the abnormal chromosome behavior during meiosis of L. hybrids pollen mother cells, most of the pollen mother cells are able to complete normal meiosis and produce pollen with normal morphology, which implies that L. hybrids is an excellent material that can be used for hybrid breeding and aneuploid breeding.