Cloning, transcriptional activation, and tissue expression analysis of XsWRI1 from Xanthoceras sorbifolium

ZHANG Wei, LI Linkun, LIANG Chongjun, WANG Libing

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2025, Vol. 49 ›› Issue (2) : 23-30.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2025, Vol. 49 ›› Issue (2) : 23-30. DOI: 10.12302/j.issn.1000-2006.202309001

Cloning, transcriptional activation, and tissue expression analysis of XsWRI1 from Xanthoceras sorbifolium

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Abstract

【Objective】This study aimed to characterize the sequence identity, transcriptional activation potential, and functional domains of the WRINKLED1 (WRI1) transcription factor in Xanthoceras sorbifolium (yellowhorn), providing insights into its regulatory role in seed oil biosynthesis. 【Method】The full-length cDNA of XsWRI1 was cloned from endosperm tissues of mature yellowhorn using rapid amplification of cDNA ends (RACE). Bioinformatics tools were employed to analyze protein sequence properties. A recombinant pGBKT7-XsWRI1 vector was constructed and transformed into Y2HGold yeast cells to assess transcriptional activation activity. Tissue-specific expression patterns were quantified via quantitative real-time PCR (qRT-PCR) across the root, stem, leaf, petal, stamen and developing embryo tissues. 【Result】The XsWRI1 gene (GenBank accession: OR500287) spans 1 688 bp, encoding a hydrophilic and unstable protein of 414 amino acids. Yeast activity testing confirmed strong transcriptional activation activity of XsWRI1. Tissue-specific expression analysis revealed predominant XsWRI1 expression in developing embryos, with negligible levels in vegetative organs (root, stem, leaf) and reproductive structures (petal, stamen). Structural prediction identified two conserved AP2/EREBP DNA-binding domains (residues 76-148 and 177-238) and a nuclear localization signal. 【Conclusion】This study elucidates the molecular characteristics and tissue-specific regulatory role of XsWRI1 in yellowhorn, highlighting its potential function in lipid biosynthesis pathways. These findings establish a foundation for targeted genetic manipulation to enhance seed oil accumulation in woody oil crops.

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Xanthoceras sorbifolium / XsWRI1 / gene cloning / transcriptional activity / tissue-specific expression / seed oil biosynthesis

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ZHANG Wei , LI Linkun , LIANG Chongjun , et al. Cloning, transcriptional activation, and tissue expression analysis of XsWRI1 from Xanthoceras sorbifolium[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2025, 49(2): 23-30 https://doi.org/10.12302/j.issn.1000-2006.202309001

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