The influence of arbuscular mycorrhizal fungi (AMF) inoculation on the growth status and photosynthetic characteristics of Leucojum aestivum

WANG Wei, MU Hongna, YANG Huimin, SUN Taoze

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2025, Vol. 49 ›› Issue (3) : 205-212.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2025, Vol. 49 ›› Issue (3) : 205-212. DOI: 10.12302/j.issn.1000-2006.202309012

The influence of arbuscular mycorrhizal fungi (AMF) inoculation on the growth status and photosynthetic characteristics of Leucojum aestivum

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Abstract

【Objective】This study investigated the effects of arbuscular mycorrhizal fungi (AMF) colonization on the growth and photosynthetic characteristics of Leucojum aestivum. 【Method】Two-year-old bulbils of L. aestivum were selected as the experimental material. The experimental design consisted of five groups, three of which received single AMF inoculations [inoculated with Rhizophagus intraradices (Ri), Funneliformis mosseae (Fm), and Diversispora tortuosa (Dt)]. The fourth group was inoculated with a 1∶1∶1 mixture of the three AMF species (Mi), and the control group was inoculated with a sterilized 1∶1∶1 mixture of the AMFs. After two months, plant height, fresh and dry weight, and photosynthetic characteristics of L. aestivum were measured. 【Result】The Fm group exhibited the highest infection rate at 69.38%, while the Ri group had the lowest infection rate at 36.93%. The infection rate in the Ri group was significantly lower than those of the Fm, Dt, and Mi groups. Among the treatment groups, the Fm group showed the most plant height (43.46 cm) and the dry weight (39.94 g). Photosynthetic parameters indicated that the Ri group had the most average stomatal conductance [Gs = 0.30 mol/(m2·s), intercellular CO2 concentration (Ci = 331 μmol/mol), and transpiration rate (Tr = 3.45 mmol/(m2·s), with the transpiration rate significantly higher than in the control group (CK)]. Correlation analysis revealed a generally significant positive correlation between net photosynthetic rate (Pn) and Gs across all treatments, except for leaf stomatal conductance (Ls) and Ci. Although other photosynthetic indices exhibited correlations, no consistent pattern was observed across treatments. Membership function analysis indicated that the inoculation effect of the Mi group was the most beneficial, followed by Dt. 【Conclusion】AMF colonization effectively promotes the growth of L. aestivum. The effects on photosynthesis vary across different AMF species, with Dt and Mi significantly enhancing the photosynthetic capacity and biomass of L. aestivum. In contrast, Fm positively affected plant height but did not significantly influence photosynthetic capacity.

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Leucojum aestivum. / arbuscular mycorrhizal fungi (AMF) / growth status / photosynthetic characteristics / correlation analysis / membership function method

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WANG Wei , MU Hongna , YANG Huimin , et al. The influence of arbuscular mycorrhizal fungi (AMF) inoculation on the growth status and photosynthetic characteristics of Leucojum aestivum[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2025, 49(3): 205-212 https://doi.org/10.12302/j.issn.1000-2006.202309012

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