Biological characteristics and inhibitor screening of the pathogen: Pestalotiopsis podocarpi causing Podocarpus macrophyllus leaf blight disease

LIU Yuqian, TAN Jiajin, ZHAO Mengting, DING Qi, DAI Huizhong, LI Chuntao

Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2025, Vol. 49 ›› Issue (6) : 255-260.

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南京林业大学学报（自然科学版）
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Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2025, Vol. 49 ›› Issue (6) : 255-260. DOI: 10.12302/j.issn.1000-2006.202403007

Biological characteristics and inhibitor screening of the pathogen: Pestalotiopsis podocarpi causing Podocarpus macrophyllus leaf blight disease

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Abstract

【Objective】In order to effectively prevent and control the disease, this study aims to systematically investigate the biological characteristics of the pathogen causing Podocarpus macrophyllus leaf blight disease and screens effective fungicides.【Method】The germination rate of spores was measured under different nutritional conditions, temperatures, pH levels, and light treatments using the glass slide germination method to study the biological characteristics of Pestalotiopsis podocarpi. The four fungicides, including 75% (mass fraction) chlorothalonil, 50% (mass fraction) carbendazol, 80% (mass fraction) mancozeb, and 70% (mass fraction) thiophanate-methyl, were evaluated in vitro for their efficacy in restraining hyphal growth and conidia germination. The toxicity regression equations were fitted to calculate the EC50, and effective fungicides were screened.【Result】The host plant decoction could promote spore germination, with the optimal concentration being 10%. The spores could germinate between 15-35 ℃, with the optimal temperature at 25 ℃. The germination rate could exceed 50% at pH 6-9, peaking at pH 7. The spores were insensitive to light, with alternating light-dark treatment resulting in slightly higher germination rates than other treatments. The most effective one against hyphal growth is carbendazol with the EC50 of 0.048 9 mg/L, followed by thiophanate-methyl with the EC50 of 0.850 5 mg/L. Mancozeb and chlorothalonil are less effective against hyphal growth. Mancozeb demonstrates the strongest inhibitory effect on conidia germination with the EC50 of 0.257 3 mg/L, followed in decreasing order of effectiveness by thiophanate-methyl, chlorothalonil, carbendazol.【Conclusion】The optimal concentration of P. macrophyllus decoction for spore germination of P. podocarpi is 10%. The optimal growth temperature and pH are 25 ℃ and 7, respectively. Carbendazol and mancozeb can be used as potential fungicides for controlling podocarpus (P. macrophyllus) leaf blight disease, while their field control efficacy requires further research.

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Podocarpus macrophyllus leaf blight disease / Pestalotiopsis podocarpi / biological characteristics / fungicides screening

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LIU Yuqian , TAN Jiajin , ZHAO Mengting , et al . Biological characteristics and inhibitor screening of the pathogen: Pestalotiopsis podocarpi causing Podocarpus macrophyllus leaf blight disease[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2025, 49(6): 255-260 https://doi.org/10.12302/j.issn.1000-2006.202403007

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