Construction of molecular ID for Osmanthus fragrans cultivars based on phenotypic traits and single nucleotide polymorphisms (SNPs)

doi:10.12302/j.issn.1000-2006.202405026

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2024, Vol. 48 ›› Issue (4): 12-24.doi: 10.12302/j.issn.1000-2006.202405026

Special Issue: 专题报道Ⅰ：郑万钧先生诞辰120周年纪念专题Ⅱ

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Construction of molecular ID for Osmanthus fragrans cultivars based on phenotypic traits and single nucleotide polymorphisms (SNPs)

WANG Yihan¹(), LIU Jiaojiao¹, JIN Peiquan¹, LI Shuqing¹, WEI Jianfen², GUO Peng¹, SHANG Fude¹^,^*()

1. College of Life Sciences, Henan Agricultural University, Henan Engineering Research Center for Osmanthus Germplasm Innovation and Resource Utilization, Zhengzhou 450046, China
2. National Osmanthus Germplasm Resource Repository, Hangzhou 310020, China

Received:2024-05-15 Revised:2024-06-07 Online:2024-07-30 Published:2024-08-05
Contact: SHANG Fude E-mail:yihanwang@vip.163.com;shangfude@henau.edu.cn

Abstract

Abstract:

【Objective】This study selected core genomic single-nucleotide polymorphism (SNP) loci to establish a rapid SNP genotyping method on the KASP platform, and to construct molecular IDs for Osmanthus fragrans cultivars. This study provides a theoretical foundation for identifying, tracing and protecting the intellectual property of O. fragrans cultivars.【Method】Field surveys were conducted to investigate key phenotypic characteristics of O. fragrans cultivars. Following two rounds of rigorous screening, we identified a set of core SNP markers capable of completely distinguishing previously sequenced cultivars. Subsequently, we analyzed the polymorphic information content (PIC) and expected heterozygosity (H_e) of each SNP locus. Using the genome sequences of ‘Rixianggui’ as a reference, species-specific KASP primers were designed for PCR amplification. Based on the genotyping results, we constructed cultivar DNA fingerprints and assessed the efficiency of core SNP markers for cultivar identification. Molecular IDs for O. fragrans cultivars were established by integrating phenotypic information codes with molecular fingerprint codes.【Result】We retained a total of 14 core SNP loci from genomic SNPs that fully discriminated the sequenced cultivars. The PIC values of these loci ranged from 0.246 to 0.375, with an average of 0.335, and the H_e indices ranged from 0.288 to 0.500, averaging 0.431. The KASP primers designed for these core SNP loci produced accurate genotyping results, enabling us to construct DNA fingerprints capable of distinguishing all 90 tested cultivars, including those not previously sequenced. Each cultivar was assigned a molecular ID composed of 34 digits.【Conclusion】In conclusion, 14 core SNP loci (SNP1 to SNP14) were identified that effectively discriminate among at least 90 O. fragrans cultivars. Unique molecular ID codes were constructed using DNA fingerprint codes along with serial codes derived from cultivar group types and phenotypic characteristics. Finally, barcode and quick response (QR) codes were generated for each cultivar.

Key words: Osmanthus fragrans, cultivar identification, single nucleotide polymorphism (SNP), phenotype, molecular ID

CLC Number:

WANG Yihan, LIU Jiaojiao, JIN Peiquan, LI Shuqing, WEI Jianfen, GUO Peng, SHANG Fude. Construction of molecular ID for Osmanthus fragrans cultivars based on phenotypic traits and single nucleotide polymorphisms (SNPs)[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(4): 12-24.

Figures/Tables 10

Supplemental Table 1

Germplasm resources information of 90 Osmanthus fragrans cultivars used in this study"

编号 No.	采样地点 sampling sites	品种名称 cultivar name	品种群 cultivar group	英国皇家园艺学会比色卡比值 RHSCC	花色 flower color	花香 floral fragrance	新梢顶芽和腋芽总数 number of terminal and axillary buds per shoot	每花序小花数量 number of flowers per inflorescence	结实性 fertility
1	河南省信阳市潢川金桂园	橙香丹桂	丹桂	23A	橙	微香	18.00	6.44	否
2	河南省信阳市潢川金桂园	潢川金桂	金桂	10B	黄	中香	21.14	6.28	是
3	河南省开封市河南大学金明校区	鄂橙	丹桂	N25B	橙	中香	22.11	5.89	否
4	河南省开封市河南大学金明校区	娇容	丹桂	23A	橙	中香	15.33	4.89	否
5	河南省开封市河南大学金明校区	象山丹桂	丹桂	N25B	橙	浓香	21.56	5.00	是
6	河南省开封市河南大学金明校区	金狮桂	金桂	6C	黄	中香	23.00	7.00	否
7	浙江省杭州市余杭区国家桂花种质资源库	齿丹桂	丹桂	25B	橙	微香	11.44	5.11	否
8	浙江省杭州市余杭区国家桂花种质资源库	橙红丹桂	丹桂	28A	橙	中香	13.56	6.11	是
9	浙江省杭州市余杭区国家桂花种质资源库	红桂	丹桂	25A	橙	中香	22.33	6.78	是
10	浙江省杭州市余杭区国家桂花种质资源库	火炼金丹	丹桂	29A	橙	微香	14.22	6.89	否
11	浙江省杭州市余杭区国家桂花种质资源库	莲籽丹桂	丹桂	25B	橙	微香	21.56	6.11	否
12	浙江省杭州市余杭区国家桂花种质资源库	满条红	丹桂	N25B	橙	中香	11.00	6.89	否
13	浙江省杭州市余杭区国家桂花种质资源库	平脉丹桂	丹桂	N25B	橙	中香	12.22	6.56	否
14	浙江省杭州市余杭区国家桂花种质资源库	软叶丹桂	丹桂	24A	橙	中香	9.44	4.89	否
15	浙江省杭州市余杭区国家桂花种质资源库	上海丹桂	丹桂	23A	橙	中香	20.33	5.89	否
16	浙江省杭州市余杭区国家桂花种质资源库	桃叶丹桂	丹桂	23A	橙	中香	8.22	9.44	否
17	浙江省杭州市余杭区国家桂花种质资源库	晚霞	丹桂	25A	橙	中香	14.00	6.22	否
18	浙江省杭州市余杭区国家桂花种质资源库	武夷丹桂	丹桂	25A	橙	微香	20.11	6.11	否
19	浙江省杭州市余杭区国家桂花种质资源库	雄黄桂	丹桂	26A	橙	微香	11.67	6.56	否
20	浙江省杭州市余杭区国家桂花种质资源库	小叶丹桂	丹桂	24A	橙	中香	26.00	8.33	否
21	浙江省杭州市余杭区国家桂花种质资源库	堰虹桂	丹桂	25A	橙	中香	13.33	6.00	否
22	浙江省杭州市余杭区国家桂花种质资源库	硬叶丹桂	丹桂	23B	橙	中香	23.33	5.89	否
23	浙江省杭州市余杭区国家桂花种质资源库	醉肌红	丹桂	25B	橙	中香	22.11	5.33	否
24	浙江省杭州市余杭区国家桂花种质资源库	朱砂丹桂	丹桂	25A	橙	微香	31.11	5.89	否
25	浙江省杭州市余杭区国家桂花种质资源库	状元红	丹桂	28B	橙	中香	11.33	5.11	否
26	浙江省杭州市余杭区国家桂花种质资源库	编金桂	金桂	10A	黄	浓香	10.00	6.22	否
27	浙江省杭州市余杭区国家桂花种质资源库	长瓣金桂	金桂	14C	黄	中香	11.00	6.33	否
28	浙江省杭州市余杭区国家桂花种质资源库	长柄金桂	金桂	12D	白	中香	20.44	6.56	否
29	浙江省杭州市余杭区国家桂花种质资源库	丛中笑	金桂	13C	黄	中香	22.33	8.00	是
30	浙江省杭州市余杭区国家桂花种质资源库	大花金桂	金桂	12C	黄	浓香	20.22	5.11	否
31	浙江省杭州市余杭区国家桂花种质资源库	大叶黄	金桂	11B	黄	浓香	7.89	5.00	否
32	浙江省杭州市余杭区国家桂花种质资源库	大叶金桂	金桂	11C	黄	浓香	24.67	8.56	是
33	浙江省杭州市余杭区国家桂花种质资源库	芙蓉桂	金桂	3C	黄	中香	31.44	6.00	否
34	浙江省杭州市余杭区国家桂花种质资源库	杭州黄	金桂	2D	黄	中香	9.22	4.89	否
35	浙江省杭州市余杭区国家桂花种质资源库	黑珍珠	金桂	2D	黄	微香	22.00	6.44	否
36	浙江省杭州市余杭区国家桂花种质资源库	金桂河边	金桂	7A	黄	中香	20.11	5.89	否
37	浙江省杭州市余杭区国家桂花种质资源库	金球桂	金桂	4C	黄	中香	37.89	6.00	否
38	浙江省杭州市余杭区国家桂花种质资源库	金秋早	金桂	1C	白	中香	32.56	6.33	否
39	浙江省杭州市余杭区国家桂花种质资源库	镰叶金桂	金桂	1D	黄	微香	39.33	6.22	否
40	浙江省杭州市余杭区国家桂花种质资源库	柳叶黄	金桂	4C	黄	中香	13.11	6.00	否
41	浙江省杭州市余杭区国家桂花种质资源库	柳叶金桂	金桂	10A	黄	中香	25.00	4.89	否
42	浙江省杭州市余杭区国家桂花种质资源库	柳叶苏桂	金桂	5C	黄	浓香	33.00	8.56	否
43	浙江省杭州市余杭区国家桂花种质资源库	漓锗金桂	金桂	11A	黄	中香	10.33	8.11	否
44	浙江省杭州市余杭区国家桂花种质资源库	球桂	金桂	7D	黄	中香	12.11	8.89	否
45	浙江省杭州市余杭区国家桂花种质资源库	软叶金桂	金桂	5C	黄	中香	22.89	4.33	是
46	浙江省杭州市余杭区国家桂花种质资源库	山茶金桂	金桂	4D	白	中香	8.78	5.44	否
47	浙江省杭州市余杭区国家桂花种质资源库	速生金桂	金桂	3C	黄	浓香	36.22	7.00	否
48	浙江省杭州市余杭区国家桂花种质资源库	小花金桂	金桂	4D	白	浓香	22.33	4.44	否
49	浙江省杭州市余杭区国家桂花种质资源库	湘金	金桂	15B	黄	中香	12.33	5.00	否
50	浙江省杭州市余杭区国家桂花种质资源库	小叶金桂	金桂	5C	黄	中香	6.56	8.33	是
51	浙江省杭州市余杭区国家桂花种质资源库	圆瓣金桂	金桂	10B	黄	中香	36.11	5.33	否
52	浙江省杭州市余杭区国家桂花种质资源库	柱冠金桂	金桂	9A	黄	浓香	8.44	6.11	是
53	浙江省杭州市余杭区国家桂花种质资源库	早秋红	金桂	163C	黄	中香	32.11	6.90	是
54	浙江省杭州市余杭区国家桂花种质资源库	橙黄四季桂	四季桂	13C	黄	浓香	19.00	8.00	否
55	浙江省杭州市余杭区国家桂花种质资源库	淡妆	四季桂	4C	黄	微香	22.89	5.44	是
56	浙江省杭州市余杭区国家桂花种质资源库	墨宝香	四季桂	7C	黄	中香	10.78	6.78	否
57	浙江省杭州市余杭区国家桂花种质资源库	日香桂黄	四季桂	4A	黄	中香	22.33	6.00	否
58	浙江省杭州市余杭区国家桂花种质资源库	日香桂	四季桂	4C	黄	浓香	26.89	8.00	否
59	浙江省杭州市余杭区国家桂花种质资源库	四季桂	四季桂	4A	黄	中香	11.11	6.00	否
60	浙江省杭州市余杭区国家桂花种质资源库	天女散花	四季桂	7B	黄	中香	20.11	5.67	否
61	浙江省杭州市余杭区国家桂花种质资源库	天香台阁	四季桂	11A	黄	中香	11.11	6.67	否
62	浙江省杭州市余杭区国家桂花种质资源库	圆叶四季桂	四季桂	3C	黄	微香	9.11	5.11	否
63	浙江省杭州市余杭区国家桂花种质资源库	白洁	银桂	1D	白	微香	8.67	5.00	是
64	浙江省杭州市余杭区国家桂花种质资源库	波叶银桂	银桂	2D	黄	中香	10.11	4.89	否
65	浙江省杭州市余杭区国家桂花种质资源库	柴桂	银桂	2A	黄	中香	10.56	4.78	否
66	浙江省杭州市余杭区国家桂花种质资源库	长叶碧珠	银桂	5A	黄	中香	21.89	4.67	否
67	浙江省杭州市余杭区国家桂花种质资源库	串银球	银桂	4D	白	浓香	22.33	8.67	否
68	浙江省杭州市余杭区国家桂花种质资源库	长叶银桂	银桂	5D	黄	微香	13.11	5.33	否
69	浙江省杭州市余杭区国家桂花种质资源库	大叶银桂	银桂	5D	黄	中香	10.33	2.89	是
70	浙江省杭州市余杭区国家桂花种质资源库	宽叶籽银	银桂	6D	黄	中香	23.00	5.33	是
71	浙江省杭州市余杭区国家桂花种质资源库	绿梗籽银	银桂	4C	黄	中香	33.44	6.00	是
72	浙江省杭州市余杭区国家桂花种质资源库	柳叶桂	银桂	6C	黄	中香	24.11	6.56	否
73	浙江省杭州市余杭区国家桂花种质资源库	密结银桂	银桂	8B	黄	浓香	21.22	5.11	否
74	浙江省杭州市余杭区国家桂花种质资源库	青尖	银桂	11A	黄	浓香	22.78	6.67	否
75	浙江省杭州市余杭区国家桂花种质资源库	速生银桂	银桂	3C	黄	浓香	11.22	6.11	否
76	浙江省杭州市余杭区国家桂花种质资源库	晚银桂	银桂	8D	黄	中香	19.00	6.11	否
77	浙江省杭州市余杭区国家桂花种质资源库	尾叶银桂	银桂	12B	黄	浓香	25.89	8.11	否
78	浙江省杭州市余杭区国家桂花种质资源库	秀丽银桂	银桂	7B	黄	浓香	23.33	7.11	否
79	浙江省杭州市余杭区国家桂花种质资源库	香云	银桂	4C	黄	微香	32.56	4.56	否
80	浙江省杭州市余杭区国家桂花种质资源库	小叶苏桂	银桂	4B	黄	浓香	23.00	8.78	否
81	浙江省杭州市余杭区国家桂花种质资源库	玉帘碧珠	银桂	1D	黄	中香	7.33	6.11	否
82	浙江省杭州市余杭区国家桂花种质资源库	玉玲珑	银桂	10A	黄	浓香	30.22	8.78	否
83	浙江省杭州市余杭区国家桂花种质资源库	玉帘银丝	银桂	2C	黄	中香	9.89	6.78	否
84	浙江省杭州市余杭区国家桂花种质资源库	杨梅叶银桂	银桂	13B	黄	浓香	10.56	5.00	否
85	浙江省杭州市余杭区国家桂花种质资源库	银星	银桂	9B	黄	中香	22.22	5.00	否
86	浙江省杭州市余杭区国家桂花种质资源库	银盏碧珠	银桂	5B	黄	中香	24.33	6.56	否
87	浙江省杭州市余杭区国家桂花种质资源库	玉柱	银桂	4A	黄	中香	19.44	6.33	否
88	浙江省杭州市余杭区国家桂花种质资源库	中华龙桂	银桂	157C	白	微香	10.78	7.67	否
89	浙江省杭州市余杭区国家桂花种质资源库	籽银桂	银桂	5B	黄	中香	27.33	8.11	是
90	浙江省杭州市余杭区国家桂花种质资源库	早银桂	银桂	10B	黄	微香	25.44	8.33	否

Supplemental Table 1

Fig. 1

Fig. 2

Table 1

Table 2

The KASP primer information for 14 core SNPs"

SNP名称 SNP name	引物编号 primer No.	引物序列 primer sequence
	SEQ ID NO.1	GAAGGTGACCAAGTTCATGCTGATCCCAGCGGAAAGCCATTTC
SNP1	SEQ ID NO.2	GAAGGTCGGAGTCAACGGATTGATCCCAGCGGAAAGCCATTTT
	SEQ ID NO.3	GATCGCCGGCGTAGCAGAAGAC
	SEQ ID NO.4	GAAGGTGACCAAGTTCATGCTTCAACTGCTGCTTTCTGATCCC
SNP2	SEQ ID NO.5	GAAGGTCGGAGTCAACGGATTCTTCAACTGCTGCTTTCTGATCCT
	SEQ ID NO.6	CATTCTTGAAATGGATTGTTCCGAGGC
	SEQ ID NO.7	GAAGGTGACCAAGTTCATGCTGAAAACCTATACGAGTCTAAGCCC
SNP3	SEQ ID NO.8	GAAGGTCGGAGTCAACGGATTTGAAAACCTATACGAGTCTAAGCCT
	SEQ ID NO.9	AATGTTACAGGAAGATGTGGCTTTATTAAAG
	SEQ ID NO.10	GAAGGTGACCAAGTTCATGCTGGTTTTTGGGGTTCTGCTTTCG
SNP4	SEQ ID NO.11	GAAGGTCGGAGTCAACGGATTCATGGTTTTTGGGGTTCTGCTTTCA
	SEQ ID NO.12	GGCAAGTCCAATGAAGAATGTTTCCAG
	SEQ ID NO.13	GAAGGTGACCAAGTTCATGCTGATGAACAAGGTTGGGTACATACC
SNP5	SEQ ID NO.14	GAAGGTCGGAGTCAACGGATTGATGAACAAGGTTGGGTACATACG
	SEQ ID NO.15	CCCTCGTCATCAAAATAGCCAAGATC
	SEQ ID NO.16	GAAGGTGACCAAGTTCATGCTAGCAAGTCCATGCAGTATCCTC
SNP6	SEQ ID NO.17	GAAGGTCGGAGTCAACGGATTCTAGCAAGTCCATGCAGTATCCTT
	SEQ ID NO.18	CTTCAGAGTAATTCACAAGCAAAATTTACCC
	SEQ ID NO.19	GAAGGTGACCAAGTTCATGCTCCACATTCGCCTCGATCTCC
SNP7	SEQ ID NO.20	GAAGGTCGGAGTCAACGGATTTACCACATTCGCCTCGATCTCT
	SEQ ID NO.21	GATTTCCCGTGGACTCCCTTACTG
	SEQ ID NO.22	GAAGGTGACCAAGTTCATGCTAGCAGATACCTTATTTACAGGTCCA
SNP8	SEQ ID NO.23	GAAGGTCGGAGTCAACGGATTGCAGATACCTTATTTACAGGTCCC
	SEQ ID NO.24	TACATTATGAGGATAAAAACACTCATTATGTATG
	SEQ ID NO.25	GAAGGTGACCAAGTTCATGCTGCACCAACTTCTGGAAGCTTAC
SNP9	SEQ ID NO.26	GAAGGTCGGAGTCAACGGATTCTGCACCAACTTCTGGAAGCTTAT
	SEQ ID NO.27	CGTCACCTCAGATGACGTCAAACTC
	SEQ ID NO.28	GAAGGTGACCAAGTTCATGCTGTCTCCGGCATTGTTTTTCCTTCA
SNP10	SEQ ID NO.29	GAAGGTCGGAGTCAACGGATTGTCTCCGGCATTGTTTTTCCTTCT
	SEQ ID NO.30	TTCAGAGCTATCTGATAAAATGGCTGAG
	SEQ ID NO.31	GAAGGTGACCAAGTTCATGCTCAAAATGTGAACTTTGTTTACATGACG
SNP11	SEQ ID NO.32	GAAGGTCGGAGTCAACGGATTATACAAAATGTGAACTTTGTTTACATGACT
	SEQ ID NO.33	GTTCCATTGTATCATCGAATATTGCAGTG
	SEQ ID NO.34	GAAGGTGACCAAGTTCATGCTGCAACATAATTATGGAGAAGATGCAAA
SNP12	SEQ ID NO.35	GAAGGTCGGAGTCAACGGATTGCAACATAATTATGGAGAAGATGCAAC
	SEQ ID NO.36	CTCCAACATGCGAGTCGAGGGC
	SEQ ID NO.37	GAAGGTGACCAAGTTCATGCTTGGTGAAGAAGTAATGGAGTTTGTC
SNP13	SEQ ID NO.38	GAAGGTCGGAGTCAACGGATTAATGGTGAAGAAGTAATGGAGTTTGTT
	SEQ ID NO.39	AAGGTGGGACCAAACCGAGCAC
	SEQ ID NO.40	GAAGGTGACCAAGTTCATGCTCCAATATACAGGACGCTGAATTGAT
SNP14	SEQ ID NO.41	GAAGGTCGGAGTCAACGGATTCCAATATACAGGACGCTGAATTGAG
	SEQ ID NO.42	GCACCAATCACTTCTGGATCATTGAATG

Table 2

Fig. 3

Fig. 4

Fig. 5

Table 3

Conversion of information and fingerprint code for 90 O. fragrans cultivars"

编号 code	品种 cultivar	品种群编码 cultivar group code	品种特征编码 information code	指纹码 fingerprint code
1	‘橙香丹桂’‘Chengxiang Dangui’	3	321120	2233211133214413334124133442
2	‘潢川金桂’‘Huangchuan Jingui’	1	112231	1133211133223411344144133442
3	‘鄂橙’‘Echeng’	3	322210	2133211133224411344144133322
4	‘娇容’‘Jiaorong’	3	322110	2134211133213411334124134422
5	‘象山丹桂’‘Xiangshan Dangui’	3	323221	1133221132223411444144133322
6	‘金狮桂’‘Jinshigui’	1	112220	2144211133113311334444333422
7	‘齿丹桂’‘Chidangui’	3	321110	2134211132214413341124133322
8	‘橙红丹桂’‘Chenghong Dangui’	3	322121	1133222122113311331122333442
9	‘红桂’‘Honggui’	3	322221	2133211133214413334124133442
10	‘火炼金丹’‘Huolian Jindan’	3	321120	2134212133213411344424113422
11	‘莲籽丹桂’‘Lianzi Dangui’	3	321220	1134211133213411334424333442
12	‘满条红’‘Mantiaohong’	3	322120	2133211133214413334422133342
13	‘平脉丹桂’‘Pingmai Dangui’	3	322120	2134222132223311341144333342
14	‘软叶丹桂’‘Ruanye Dangui’	3	322110	2133211133224411344124113322
15	‘上海丹桂’‘Shanghai Dangui’	3	322210	2134222233114413344122134442
16	‘桃叶丹桂’‘Taoye Dangui’	3	322130	2234211133214413341122133322
17	‘晚霞’‘Wanxia’	3	322120	2134212133213411334124113422
18	‘武夷丹桂’‘Wuyi Dangui’	3	321220	2134211133213411334124134442
19	‘雄黄桂’‘Xionghuanggui’	3	321120	1133221132213411331124333442
20	‘小叶丹桂’‘Xiaoye Dangui’	3	322230	1133221132223411444444333322
21	‘堰虹桂’‘Yanhonggui’	3	322120	1133222133113311331122333442
22	‘硬叶丹桂’‘Yingye Dangui’	3	322210	2234222132223411334124133442
23	‘醉肌红’‘Zuijihong’	3	322210	2133211133224411344122133322
24	‘朱砂丹桂’‘Zhusha Dangui’	3	321310	2134211133223411331144333422
25	‘状元红’‘Zhuangyuanhong’	3	322110	2134211133223411334144333422
26	‘编金桂’‘Bianjingui’	1	113120	2234222133213411334424333442
27	‘长瓣金桂’‘Changban Jingui’	1	112120	2144221132223413331144333344
28	‘长柄金桂’‘Changbing Jingui’	1	102220	2233222133213411331124333444
29	‘丛中笑’‘Congzhongxiao’	1	112221	2133222132223411341144333342
30	‘大花金桂’‘Dahua Jingui’	1	113210	2234211133223411334124134442
31	‘大叶黄’‘Dayehuang’	1	113120	2233222133223411331124333444
32	‘大叶金桂’‘Daye Jingui’	1	113231	2133222233213413334122113342
33	‘芙蓉桂’‘Furonggui’	1	112320	2133222133223313334124134442
34	‘杭州黄’‘Hangzhouhuang’	1	112110	1144212133224413341124133422
35	‘黑珍珠’‘Heizhenzhu’	1	111220	2233222133213413334144133442
36	‘金桂河边’‘Jingui Hebian’	1	112210	1133212133113311344422134442
37	‘金球桂’‘Jinqiugui’	1	112320	2134212233213411344124113422
38	‘金秋早’‘Jinqiuzao’	1	102320	1133222133113313334124334422
39	‘镰叶金桂’‘Lianye Jingui’	1	111320	2133221133223411334144114442
40	‘柳叶黄’‘Liuyehuang’	1	112120	2233221133213333334424113444
41	‘柳叶金桂’‘Liuye Jingui’	1	112210	2233221133223411334144113442
42	‘柳叶苏桂’‘Liuye Sugui’	1	113330	2233221133214411334124334422
43	‘漓锗金桂’‘Lizhe Jingui’	1	112130	1133222133113311344424134442
44	‘球桂’‘Qiugui’	1	112130	1134222132223411344144134422
45	‘软叶金桂’‘Ruanye Jingui’	1	112211	2233221132213313331144133444
46	‘山茶金桂’‘Shancha Jingui’	1	102110	2133222133214411334422334422
47	‘速生金桂’‘Susheng Jingui’	1	113320	1133112133113311344424134442
48	‘小花金桂’‘Xiaohua Jingui’	1	103210	1134212132213411444124333422
49	‘湘金’‘Xiangjin’	1	112120	2134212133213411344124113422
50	‘小叶金桂’‘Xiaoye Jingui’	1	112131	2133222233213411334444334422
51	‘圆瓣金桂’‘Yuanban Jingui’	1	112310	2133222133214411334424334422
52	‘柱冠金桂’‘Zhuguan Jingui’	1	113121	1144212232223411331124334422
53	‘早秋红’‘Zaoqiuhong’	1	112321	2234221132113413334124134442
54	‘橙黄四季桂’‘Chenghuang Sijigui’	4	413120	2133221132213411334124333322
55	‘淡妆’‘Danzhuang’	4	411211	1144221133213433331144333342
56	‘墨宝香’‘Mobaoxiang’	4	412120	1134222132223411334144333442
57	‘日香桂黄’‘Rixiang Guihuang’	4	412220	1134222222223311331144134444
58	‘日香桂’‘Rixianggui’	4	413220	2133222233213311334422113342
59	‘四季桂’‘Sijigui’	4	412120	2133211132214413344124134442
60	‘天女散花’‘Tiannv Sanhua’	4	412210	2134212133223413344444333422
61	‘天香台阁’‘Tianxiang Taige’	4	412120	2133222132223411334124333422
62	‘圆叶四季桂’‘Yuanye Sijigui’	4	411110	2234211132223413344144134442
63	‘白洁’‘Baijie’	2	201121	2244212133114413344424333444
64	‘波叶银桂’‘Boye Yingui’	2	212110	2134222233213411334122113342
65	‘柴桂’‘Chaigui’	2	212110	2234222132213313341144334444
66	‘长叶碧珠’‘Changye Bizhu’	2	212210	2144222133224411334444333444
67	‘串银球’‘Chuanyinqiu’	2	203230	2134222232213311331124333442
68	‘长叶银桂’‘Changye Yingui’	2	211110	2233222132223411334124133442
69	‘大叶银桂’‘Daye Yingui’	2	212111	2134221133214413334124133422
70	‘宽叶籽银’‘Kuanye Ziyin’	2	212211	2144221133213411334144333344
71	‘绿梗籽银’‘Lvgeng Ziyin’	2	212321	1134221133224413334124133322
72	‘柳叶桂’‘Liuyegui’	2	212220	1144221133214411441124333422
73	‘密结银桂’‘Mijie Yingui’	2	213210	1134222122223313334122133442
74	‘青尖’‘Qingjian’	2	213220	2134221132214411344144134422
75	‘速生银桂’‘Susheng Yingui’	2	213120	2134212133213411344124113442
76	‘晚银桂’‘Wanyingui’	2	212120	2234222133214413334124133342
77	‘尾叶银桂’‘Weiye Yinggui’	2	213230	2234112133213411344124113422
78	‘秀丽银桂’‘Xiuli Yingui’	2	213220	1144212133223311331124334422
79	‘香云’‘Xiangyun’	2	211310	1144222133224411334444333444
80	‘小叶苏桂’‘Xiaoye Sugui’	2	213230	1133211133224411344444113422
81	‘玉帘碧珠’‘Yulian Bizhu’	2	212120	1134222132213333334424133342
82	‘玉玲珑’‘Yulinglong’	2	213330	1134211133224411344444113422
83	‘玉帘银丝’‘Yulian Yinsi’	2	212120	2133222233213411334422113342
84	‘杨梅叶银桂’‘Yangmeiye Yingui’	2	213120	2133221133223411334144113442
85	‘银星’‘Yinxing’	2	212220	2134222233113413334122113342
86	‘银盏碧珠’‘Yinzhan Bizhu’	2	212220	2233221133213411334122133422
87	‘玉柱’‘Yuzhu’	2	212120	2233222132213413334124133444
88	‘中华龙桂’‘Zhonghua Longgui’	2	201120	1134222132223313334444333442
89	‘籽银桂’‘Ziyingui’	2	212231	2234221133224411441124133422
90	‘早银桂’‘Zaoyingui’	2	211230	2234112233223411334122334444

Table 3

Fig. S1

References 57

[1]	赵宏波, 郝日明, 胡绍庆. 中国野生桂花的地理分布和种群特征[J]. 园艺学报, 2015, 42(9):1760-1770.
	ZHAO H B, HAO R M, HU S Q. Geographic distribution and population characteristics of Osmanthus fragrans[J]. Acta Hortic Sin, 2015, 42(9):1760-1770.DOI: 10.16420/j.issn.0513-353x.2014-0939.
[2]	向其柏, 刘玉莲. 中国桂花品种图志[M]. 杭州: 浙江科学技术出版社, 2008.
	XIANG Q B, LIU Y L. An illustrated monograph of the sweet osmanthus cultivars in China[M]. Hangzhou: Zhejiang Science & Technology Press, 2008.
[3]	杨康民. 中国桂花[M]. 北京: 中国林业出版社, 2013.
	YANG K M. Chinese osmanthus[M]. Beijing: China Forestry Publishing House, 2013.
[4]	向民, 段一凡, 向其柏. 木犀属品种国际登录中心年报(1) 彩叶桂品种群的建立[J]. 南京林业大学学报(自然科学版), 2014, 38(1):2,187.
	XIANG M, DUAN Y F, XIANG Q B. International Cultivar Registration Center for Osmanthus,Nanjing Forestry University.Establishment of a new group-Osmanthus fragrans Colour Group[J]. J Nanjing For Univ (Nat Sci Ed), 2014, 38(1):2,187.DOI: 10.3969/j.issn.1000-2006.2014.01.034.
[5]	高丽, 何岳球, 孙志国, 等. 三个 “新发展” 下咸宁市中国桂花城传承创新发展研究[J]. 安徽农业科学, 2023, 51(12):256-259.
	GAO L, HE Y Q, SUN Z G, et al. Research on the inheritance,innovation and high-quality development of Chinese Osmanthus city in Xianning under three “new developments”[J]. J Anhui Agric Sci, 2023, 51(12):256-259.DOI: 10.3969/j.issn.0517-6611.2023.12.058.
[6]	刘燕培, 李书情, 王长海, 等. 桂花新品种‘潢川丹桂’[J]. 园艺学报, 2022, 49(S2):229-230.
	LIU Y P, LI S Q, WANG C H, et al. A new Osmanthus fragrans cultivar ‘Huangchuan Dangui’[J]. Hortic Plant J, 2022, 49(S2):229-230. DOI: 10.16420/j.issn.0513-353x.2022-0376. (in Chinese with English abstract)
[7]	王良桂, 潘多, 丁卉芬, 等. 彩叶桂新品种‘南林彩云’[J]. 南京林业大学学报(自然科学版), 2023, 47(2):243-244.
	WANG L G, PAN D, DING H F, et al. Osmanthus fragrans ‘Nanlin Caiyun’:a new cultivar of Osmanthus[J]. J Nanjing For Univ (Nat Sci Ed), 2023, 47(2):243-244.
[8]	国家质量监督检验检疫总局, 中国国家标准化管理委员会. 植物新品种特异性、一致性、稳定性测试指桂花:GB/T 24885—2010[S]. 北京: 中国标准出版社, 2011.
	General Administration of Quality Supervision,Inspection and Quarantine of the People’s Republic of China, Standardization Administration of the People’s Republic of China. Guidelines for the conduct of test for distinctness,uniformity and stability-Sweet osmanthus(Osmanthus fragrans L.):GB/T 24885-2010[S]. Beijing: Standards Press of China, 2011.
[9]	UPOV. TGP/15. Guidance on the use of biochemical and molecular markers in the examination of distinctness,uniformity and stability(DUS). Geneva:UPOV, 2013.
[10]	李梅. 桂花种质资源遗传多样性研究及品种鉴定[D]. 南京: 南京农业大学, 2009.
	LI M. Study on genetic diversity and cultivar identification of Osmanthus fragrans germplasm[D]. Nanjing: Nanjing Agricultural University, 2009.
[11]	邱帅, 吴光洪, 陈徐平, 等. 基于相关序列扩增多态性分子标记的桂花栽培品种演化分析[J]. 浙江大学学报(农业与生命科学版), 2017, 43(4):404-415.
	QIU S, WU G H, CHEN X P, et al. Evolution analysis of sweet Osmanthus(Osmanthus fragrans) cultivars based on sequence-related amplified polymorphism molecular marker[J]. J Zhejiang Univ (Agric Life Sci), 2017, 43(4):404-415.DOI: 10.3785/j.issn.1008-9209.2016.08.241.
[12]	乔中全, 王晓明, 李永欣, 等. 桂花优良品种‘珍珠彩桂’遗传多样性的ISSR分析及指纹图谱构建[J]. 湖南林业科技, 2016, 43(3):1-5.
	QIAO Z Q, WANG X M, LI Y X, et al. Genetic diversity and fingerprint construction of varieties of Osmanthus fragrans ‘Zhenzhu Caigui’ by ISSR markers[J]. Hunan For Sci Technol, 2016, 43(3):1-5.DOI: 10.3969/j.issn.1003-5710.2016.03.001.
[13]	罗仙英, 桂敬飞, 严治, 等. 贵州桂花种质资源遗传多样性的ISSR分析[J]. 种子, 2017, 36(6):62-66.
	LUO X Y, GUI J F, YAN Z, et al. Genetic diversity analyses of Osmanthus fragrans lour.in Guizhou with ISSR markers[J]. Seed, 2017, 36(6):62-66.DOI: 10.16590/j.cnki.1001-4705.2017.06.062.
[14]	韩远记. 桂花品种资源遗传多样性的AFLP分析[D]. 开封: 河南大学, 2008.
	HAN Y J. Study on the genetic diversity of Osmanthus fragrans cultivars by AFLP markers[D]. Kaifeng: Henan University, 2008.
[15]	YAN X Y, XIAO B L, HAN Y J, et al. AFLP analysis of genetic relationships and diversity of some Chinese Osmanthus fragrans cultivars[J]. Life Sci J, 2009, 6(2):11-16.
[16]	ZHANG Z R, FAN D M, GUO S Q, et al. Development of 29 microsatellite markers for Osmanthus fragrans (Oleaceae),a traditional fragrant flowering tree of China[J]. Am J Bot, 2011, 98(12):e356-e359.DOI: 10.3732/ajb.1100241.
[17]	DUAN Y F, WANG X R, XIANG Q B, et al. Genetic diversity of androdioecious Osmanthus fragrans (Oleaceae) cultivars using microsatellite markers[J]. Appl Plant Sci, 2013, 1(6): apps.1200092.DOI: 10.3732/apps.1200092.
[18]	李军, 董彬, 张超, 等. 桂花EST-SSR引物开发及在品种鉴定中的应用[J]. 浙江农林大学学报, 2018, 35(2):306-313.
	LI J, DONG B, ZHANG C, et al. EST-SSR primers and their application in cultivar identification of Osmanthus fragrans[J]. J Zhejiang A F Univ, 2018, 35(2):306-313.DOI: 10.11833/j.issn.2095-0756.2018.02.015.
[19]	YANG X L, YUE Y Z, LI H Y, et al. The chromosome-level quality genome provides insights into the evolution of the biosynthesis genes for aroma compounds of Osmanthus fragrans[J]. Hortic Res, 2018, 5:72.DOI: 10.1038/s41438-018-0108-0.
[20]	CHEN H G, ZENG X L, YANG J, et al. Whole-genome resequencing of Osmanthus fragrans provides insights into flower color evolution[J]. Hortic Res, 2021, 8(1):98.DOI: 10.1038/s41438-021-00531-0.
[21]	LI Y, ZHAO H, XIA H X, et al. Multi-omics analyses provide insights into the genomic basis of differentiation among four sweet osmanthus groups[J]. Plant Physiol, 2024:kiae280.DOI: 10.1093/plphys/kiae280.
[22]	魏中艳, 李慧慧, 李骏, 等. 应用SNP精准鉴定大豆种质及构建可扫描身份证[J]. 作物学报, 2018, 44(3):315-323.
	WEI Z Y, LI H H, LI J, et al. Accurate identification of varieties by nucleotide polymorphisms and establishment of scannable variety IDs for soybean germplasm[J]. Acta Agron Sin, 2018, 44(3):315-323.DOI: 10.3724/SP.J.1006.2018.000315.
[23]	DU H S, YANG J J, CHEN B, et al. Target sequencing reveals genetic diversity,population structure,core-SNP markers,and fruit shape-associated loci in pepper varieties[J]. BMC Plant Biol, 2019, 19(1):578.DOI: 10.1186/s12870-019-2122-2.
[24]	BUTTON P. The international union for the protection of new varieties of plants (upov) recommendations on variety denominations[J]. Acta Hortic, 2008(799):191-200.DOI: 10.17660/actahortic.2008.799.27.
[25]	HEO M S, HAN K, KWON J K, et al. Development of SNP markers using genotyping-by-sequencing for cultivar identification in rose (Rosa hybrida)[J]. Hortic Environ Biotechnol, 2017, 58(3):292-302.DOI: 10.1007/s13580-017-0268-0.
[26]	贾清香. 基于RAD测序的野鸢尾和射干的SSR及SNP特征分析[D]. 沈阳: 沈阳农业大学, 2018.
	JIA Q X. Characteristics of SSR and SNP in I. dichotoma and I. domestica using RAD sequencing[D]. Shenyang: Shenyang Agricultural University, 2018.
[27]	MARTINA M, ACQUADRO A, PORTIS E, et al. Diversity analyses in two ornamental and large-genome Ranunculaceae species based on a low-cost Klenow NGS-based protocol[J]. Front Plant Sci, 2023, 14:1187205.DOI: 10.3389/fpls.2023.1187205.
[28]	SEMAGN K, BABU R, HEARNE S, et al. Single nucleotide polymorphism genotyping using Kompetitive Allele Specific PCR (KASP):Overview of the technology and its application in crop improvement[J]. Mol Breed, 2014, 33(1):1-14.DOI: 10.1007/s11032-013-9917-x.
[29]	HE C L, HOLME J, ANTHONY J. SNP genotyping: the KASP assay[M]. New York: Humana Press, 2014:75-86. DOI: 10.1007/978-1-4939-0446-4_7.
[30]	GANAL M W, WIESEKE R, LUERSSEN H, et al. High-throughput SNP profiling of genetic resources in crop plants using genotyping arrays[M]// Genom Plant Genet Resour. Dordrecht: Springer, 2014:113-130. DOI: 10.1007/978-94-007-7572-5_6.
[31]	DO KIM K, KANG Y N, KIM C. Application of genomic big data in plant breeding:past,present,and future[J]. Plants, 2020, 9(11):1454.DOI: 10.3390/plants9111454.
[32]	NGUYENen, TOAN K, HA S T T, et al. Analysis of Chrysanthemum genetic diversity by genotyping-by-sequencing[J]. Hortic Environ Biotechnol, 2020, 61:903-913. DOI: 10.1007/s13580-020-00274-2.
[33]	WANG F Q, FAN X C, ZHANG Y, et al. Establishment and application of an SNP molecular identification system for grape cultivars[J]. J Integr Agric, 2022, 21(4):1044-1057.DOI: 10.1016/S2095-3119(21)63654-7.
[34]	LI Z Y, YU H L, LI X, et al. Kompetitive allele-specific PCR (KASP) genotyping and heterotic group classification of 244 inbred lines in cabbage (Brassica oleracea L.var.capitata)[J]. Euphytica, 2020, 216(7):106.DOI: 10.1007/s10681-020-02640-8.
[35]	RASHEED A, WEN W E, GAO F M, et al. Development and validation of KASP assays for genes underpinning key economic traits in bread wheat[J]. Theor Appl Genet, 2016, 129(10):1843-1860.DOI: 10.1007/s00122-016-2743-x.
[36]	GREWAL S, HUBBART-EDWARDS S, YANG C Y, et al. Rapid identification of homozygosity and site of wild relative introgressions in wheat through chromosome-specific KASP genotyping assays[J]. Plant Biotechnol J, 2020, 18(3):743-755.DOI: 10.1111/pbi.13241.
[37]	李书情. 基于全基因组SNP的桂花品种精准鉴定体系的构建及应用[D]. 郑州: 河南农业大学, 2023.
	LI S Q. Construction and application of accurate identification system of Osmanthus fragrans varieties based on whole-genome SNP[D]. Zhengzhou: Henan Agricultural University, 2023.DOI: 10.27117/d.cnki.ghenu.2023.000780.
[38]	POREBSKI S, BAILEY L G, BAUM B R. Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components[J]. Plant Mol Biol Report, 1997, 15(1):8-15.DOI: 10.1007/BF02772108.
[39]	CAI X, MAI R Z, ZOU J J, et al. Analysis of aroma-active compounds in three sweet Osmanthus (Osmanthus fragrans) cultivars by GC-olfactometry and GC-MS[J]. J Zhejiang Univ Sci B, 2014, 15(7):638-648.DOI: 10.1631/jzus.B1400058.
[40]	FU J X, HOU D, ZHANG C, et al. The emission of the floral scent of four Osmanthus fragrans cultivars in response to different temperatures[J]. Molecules, 2017, 22(3):430.DOI: 10.3390/molecules22030430.
[41]	HAN Y J, LI L X, DONG M F, et al. cDNA cloning of the phytoene synthase (PSY) and expression analysis of PSY and carotenoid cleavage dioxygenase genes in Osmanthus fragrans[J]. Biologia, 2013, 68(2):258-263.DOI: 10.2478/s11756-013-0002-z.
[42]	路飞. 桂花新品种DUS测试指南的研制[D]. 南京: 南京林业大学, 2009.
	LU F. Study on the design of DUS testing guideline for new varieties of Osmanthus fragrans[D]. Nanjing: Nanjing Forestry University, 2009.
[43]	VOSS D H, HALE W N. A comparison of the three editions of the royal horticultural society colour chart[J]. Hortscience, 1998, 33:13-17.DOI: 10.21273/HORTSCI.33.1.13.
[44]	臧德奎, 向其柏. 中国桂花品种分类研究[J]. 中国园林, 2004, 20(11):40-49.
	ZANG D K, XIANG Q B. Studies on the cultivar classification of Chinese sweet Osmanthus[J]. J Chin Landsc Archit, 2004, 20(11):40-49.DOI: 10.3969/j.issn.1000-6664.2004.11.011.
[45]	尚富德, 陈仲芳, 刘玉莲, 等. 桂花品种资源调查方法研究[J]. 河南大学学报(自然科学版), 2003, 33(1):9-13.
	SHANG F D, CHEN Z F, LIU Y L, et al. Study on the investigation of cultivar resources of Osmanthus fragrans[J]. J Henan Univ (Nat Sci), 2003, 33(1):9-13.DOI: 10.15991/j.cnki.411100.2003.01.003.
[46]	GLEISER G, VERDÚ M. Repeated evolution of dioecy from androdioecy in Acer[J]. New Phytol, 2005, 165(2):633-640.DOI: 10.1111/j.1469-8137.2004.01242.x.
[47]	DANECEK P, AUTON A, ABECASIS G, et al. The variant call format and VCFtools[J]. Bioinformatics, 2011, 27(15):2156-2158.DOI: 10.1093/bioinformatics/btr330.
[48]	李梓榕, 袁雄, 陈叶, 等. 基于全基因组SNP高效鉴定水稻种质资源并构建指纹图谱[J]. 分子植物育种, 2020, 18(18):6050-6057.
	LI Z R, YUAN X, CHEN Y, et al. Effective identification for varieties by genome-wide SNPs and establishment of fingerprint for rice germplasm[J]. Mol Plant Breed, 2020, 18(18):6050-6057.DOI: 10.13271/j.mpb.018.006050.
[49]	SHEN Y S, WANG J S, SHAW R K, et al. Development of GBTS and KASP panels for genetic diversity,population structure,and fingerprinting of a large collection of broccoli (Brassica oleracea L.var.italica) in China[J]. Front Plant Sci, 2021, 12:655254.DOI: 10.3389/fpls.2021.655254.
[50]	LIU K J, MUSE S V. PowerMarker:An integrated analysis environment for genetic marker analysis[J]. Bioinformatics, 2005, 21(9):2128-2129.DOI: 10.1093/bioinformatics/bti282.
[51]	杨康民, 朱文江. 桂花[M]. 上海: 上海科学技术出版社, 1999.
	YANG K M, ZHU W J. Osmanthus fragrans[M]. Shanghai: Shanghai Scientific & Technical Publishers, 1999. (in Chinese)
[52]	任海龙, 许东林, 张晶, 等. 菜薹KASP-SNP指纹图谱构建及品种鉴定[J]. 园艺学报, 2023, 50(2):307-318.
	REN H L, XU D L, ZHANG J, et al. Establishment of SNP fingerprinting and identification of Chinese flowering cabbage varieties based on KASP genotyping[J]. Acta Hortic Sin, 2023, 50(2):307-318.DOI: 10.16420/j.issn.0513-353x.2021-1046.
[53]	王富强, 樊秀彩, 张颖, 等. SNP分子标记在作物品种鉴定中的应用和展望[J]. 植物遗传资源学报, 2020, 21(5):1308-1320.
	WANG F Q, FAN X C, ZHANG Y, et al. Application and prospect of SNP molecular markers in crop variety identification[J]. J Plant Genet Resour, 2020, 21(5):1308-1320.DOI: 10.13430/j.cnki.jpgr.20200309002.
[54]	LI H L, XIAO W J, TONG T, et al. The specific DNA barcodes based on chloroplast genes for species identification of Orchidaceae plants[J]. Sci Rep, 2021, 11(1):1424.DOI: 10.1038/s41598-021-81087-w.
[55]	樊晓静, 于文涛, 蔡春平, 等. 利用SNP标记构建茶树品种资源分子身份证[J]. 中国农业科学, 2021, 54(8):1751-1772.
	FAN X J, YU W T, CAI C P, et al. Construction of molecular ID for tea cultivars by using of single-nucleotide polymorphism(SNP) markers[J]. Sci Agric Sin, 2021, 54(8):1751-1772.DOI: 10.3864/j.issn.0578-1752.2021.08.014.
[56]	赵仁欣, 李森业, 郭瑞星, 等. 利用SNP芯片构建我国冬油菜参试品种DNA指纹图谱[J]. 作物学报, 2018, 44(7):956-965.
	ZHAO R X, LI S Y, GUO R X, et al. Construction of DNA fingerprinting for Brassica napus varieties based on SNP chip[J]. Acta Agron Sin, 2018, 44(7):956-965.DOI: 10.3724/SP.J.1006.2018.00956.
[57]	朱国忠, 张芳, 付洁, 等. 适于陆地棉品种身份鉴定的SNP核心位点筛选与评价[J]. 作物学报, 2018, 44(11):1631-1639.
	ZHU G Z, ZHANG F, FU J, et al. Genome-wide screening and evaluation of SNP core loci for identification of upland cotton varieties[J]. Acta Agron Sin, 2018, 44(11):1631-1639.DOI: 10.3724/SP.J.1006.2018.01631.9.

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SNP名称 SNP name	染色体 chromosome	物理位置 position	所在基因 gene	基因注释 gene annotation	变异类型 variation type	次要等位基因频率 MAF	多态信息含量 PIC	H_e
SNP1	1	2 570 938	VPS35B	液泡分选相关蛋白35B vacuolar protein sorting-associated protein 35B	TS	0.483	0.375	0.499
SNP2	2	27 947 738	E5.1.3.15	葡萄糖-6-磷酸-1-差向异构酶 glucose-6-phosphate 1-epimerase	TS	0.343	0.349	0.451
SNP3	3	24 737 420	GLU	谷氨酸合成酶 glutamate synthase	TS	0.208	0.275	0.329
SNP4	6	3 953 530	MAP65-3	微管相关蛋白(MAP65/ASE1)家族蛋白 microtubule associated protein (MAP65/ASE1) family protein	TS	0.348	0.351	0.454
SNP5	7	4 581 291	4CLL7	4-香豆酸辅酶A连接酶 4-coumarate CoA ligase	TV	0.174	0.246	0.288
SNP6	9	11 920 715	RECQL5	ATP依赖的DNA解旋酶 ATP-dependent DNA helicase	TS	0.360	0.354	0.461
SNP7	11	7 705 120	LDL1	组蛋白赖氨酸特异性去甲基化酶1 lysine-specific histone demethylase 1	TS	0.466	0.374	0.498
SNP8	13	2 4914 140	CASBPX2	环阿屯醇合成酶 cycloartenol synthase 2	TV	0.180	0.251	0.295
SNP9	14	1 455 309	CLASP	微管结合蛋白 CLIP-associated protein	TS	0.202	0.271	0.323
SNP10	15	27 882 455	ABCB19	ATP结合盒亚家族B19 ATP binding cassette subfamily B19	TV	0.489	0.375	0.500
SNP11	18	26 280 736	SCPL7	丝氨酸羧肽酶7 serine carboxypeptidase-like 7	TV	0.427	0.370	0.489
SNP12	23	10 404 919	MCM4	DNA复制许可因子MCM4 DNA replication licensing factor MCM4	TV	0.399	0.365	0.480
SNP13	23	17 820 664	SPPL4	信号肽肽酶4 signal peptide peptidase-like 4	TS	0.489	0.375	0.500
SNP14	23	20 338 374	At4g22670	热休克蛋白70 1 HSP70-interacting protein 1	TV	0.365	0.356	0.464

Construction of molecular ID for Osmanthus fragrans cultivars based on phenotypic traits and single nucleotide polymorphisms (SNPs)

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