Identification of free-pollinated progeny and optimization of pollinated variety combination of Carya illinoinensis based on SSR markers

HUANG Dan, LUO Xiaolei, PENG Bingyang, WANG Leibin, BI Huihui, HE Deming, LYU Jiabin

Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 0

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南京林业大学学报（自然科学版）
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Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 0 DOI: 10.12302/j.issn.1000-2006.202408003

Identification of free-pollinated progeny and optimization of pollinated variety combination of Carya illinoinensis based on SSR markers

  • HUANG Dan1,2, LUO Xiaolei1,2, PENG Bingyang1,2, WANG Leibin3, BI Huihui3, HE Deming3, LYU Jiabin1,2,*
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Abstract

【Objective】SSR markers were employed to identify the free-pollinated progeny of pecan, screen the full-sib progeny, and investigate the pollination affinity and effective reproductive contribution rate of various varieties. This research aims to provide a foundation for establishing reasonable pollination allocation and breeding superior individual plants in orchards.【Method】Using 333 progeny produced through the free pollination of eight varieties of excellent single mother trees in a thin shell pecan orchard, along with 36 candidate paternal pairs from the garden, based on the 12 pairs of pre-screened SSR primers, the related parameters of parental analysis were evaluated using Cervus 3.0 software, based on the maximum likelihood method. Furthermore, we conducted a real paternity identification and analyzed the effective contribution rates of eight thin shell pecan varieties: 'Mahan', 'Jinhua', 'Pawnee', 'Annong 3', 'Shaoxing', 'Annong 4', 'Elliott', and 'Wichita'.【Result】12 SSR markers could be effectively amplified and genotyped. At 80% confidence level, 321 (96.4%) progeny were identified as clear fathers. Among the 36 candidate fathers, the average reproductive contribution rate was 2.9%, and there were 14 candidate fathers whose reproductive contribution rate was greater than 3% (producing 10 progeny), producing 199 progeny in total, accounting for 59.75% of the total progeny. The highest paternal contribution rate was paternal No. 35, which produced 23 offspring, with a reproductive contribution rate of 6.9%. Based on the parental contribution rate of the experiment, the best pollinator of 'Mahan' is' Annong 3 ', the best pollinator of 'Pawnee' is' C20 'and' Guodada ', the best pollinator of 'Jinhua' is' Fukete ', the best pollinator of 'Annong 3' is' Mahan ', the best pollinator of 'Annong 4' is' C19 ', and the best pollinator of 'Shaoxing' is' Shawney '. The best pollination variety of 'Elliott' was' C17 ', and the best pollination variety of 'Wichita' was' C20 '. In addition, 8 female hickory pecans were also identified as candidate paternal parents, and no offspring from self-cross was found, indicating that hickory pecans were mostly outcrossed.【Conclusion】In this study, SSR markers were used to analyze the female free-pollinating progenies of different varieties of thin-shelled pecan, and the pollination affinity and effective reproductive contribution rate among different varieties were studied, so as to screen out the best pollination combination of each variety, providing an important basis for the selection and allocation of pollination varieties during the construction of thin-shelled pecan orchards. At the same time, it can also provide reference for the optimal allocation of other economic tree species.

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Carya illinoinensis / SSR marker / Full-sib family / Paternity analysis

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HUANG Dan, LUO Xiaolei, PENG Bingyang, WANG Leibin, BI Huihui, HE Deming, LYU Jiabin. Identification of free-pollinated progeny and optimization of pollinated variety combination of Carya illinoinensis based on SSR markers[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 0 https://doi.org/10.12302/j.issn.1000-2006.202408003

References

[1] 张日清, 吕芳德. 优良经济树种──美国山核桃[J]. 广西林业科学, 1998, (4): 202-206.
ZHANG R Q, LYU F D.Excellent economic tree species──American pecans[J]. Guangxi Forestry Science, 1998, (4): 202-206. DOI: 10.19692/j.cnki.gfs.1998.04.009.
[2] 赵喆, 王菲, 胡甜, 等. 5个薄壳山核桃品种形态及营养成分的分析[J]. 经济林研究, 2023, 41(3): 159-168.
ZHAO Z, WANG F, HU T, et al.Analysis of morphology and nutritional components of five thin shell pecan(Carya illinoinensis) cultivars[J]. Economic Forestry Research, 2023, 41(3): 159-168. DOI: 10.14067/j.cnki.1003-8981.2023.03.015.
[3] 张计育, 王刚, 王涛, 等. 薄壳山核桃果实性状和种仁脂肪酸含量多样性分析[J]. 植物资源与环境学报, 2023, 32(2): 29-37.
ZHANG J Y, WANG G, WANG T, et al.Analysis of fruit traits and diversity of fatty acids in seed kernel of Hickory pecan(Carya illinoinensis)[J]. Journal of Plant Resources and Environment, 2023, 32(2): 29-37.DOI: 10.3969/j.issn.1674-7895.2023.02.04
[4] 彭方仁, 李永荣, 郝明灼, 等. 我国薄壳山核桃生产现状与产业化发展策略[J]. 林业科技开发, 2012, 26(4): 1-4.
PENG F R, LI Y R, HAO M Z, et al.Production status and industrialization development strategy of thin shell pecan(Carya illinoinensis) in China[J]. Forestry Science and Technology Development, 2012, 26(4): 1-4. DOI: 10.3969/j.issn.1000-8101.2012.04.001
[5] 张日清, 李江, 吕芳德, 等. 我国引种美国山核桃历程及资源现状研究[J]. 经济林研究, 2003, (4): 107-109.
ZHANG R Q, LI J, LYU F D, et al.Study on the history and resources of introduction of American pecan(Carya illinoinensis) in China[J]. Economic Forest Research, 2003, (4): 107-109. DOI:10.14067／j.cnki.1003-8981.2003.04.036
[6] 吕佳斌, 翁启杰, 李发根, 等. 基于SSR标记的大花序桉全同胞子代鉴定[J]. 中南林业科技大学学报, 2021, 41(10): 148-155.
LYU J B, WENG Q J, LI F G, et al.SSR-based identification of full-sib seedlings in Eucalyptus cloeziana[J]. Journal of Central South University of Forestry and Technology, 2021, 41(10): 148-155. DOI: 10.14067/j.cnki.1673-923x.2021.10.017 http://qks.csuft.edu.cn
[7] 王瑞, 洪文娟, 罗华, 等. 石榴品种SSR指纹图谱构建及杂种父本鉴定[J]. 园艺学报, 2023, 50(2): 265-278.
WANG R, HONG W J, LUO H, et al.Construction of SSR fingerprints of pomegranate cultivars and male parent identification of hybrids[J]. Chinese Journal of Horticulture, 2023, 50(2): 265-278. DOI: 10.16420/j.issn.0513-353x.2021-1008
[8] 邵文豪, 王兆山, 张建国. 基于SSR标记的油橄榄主要栽培品种子代父本分析[J]. 林业科学研究, 2020, 33(3): 22-30.
SHAO W H, WANG Z S, ZHANG J G.Paternity analysis of main olive cultivars progenies based on SSR markers[J]. Research in Forestry Sciences, 2020, 33(3): 22-30. DOI: 10.13275/j.cnki.lykxyj.2020.03.003
[9] WANG H B, CHEN PH, YANG Y Q, et al.Molecular insights into the origin of the brown rust resistance gene Bru1 among Saccharum species[J]. Theoretical and Applied Genetics, 2017, 130:2431-2443 DOI: 10.1007/s00122-017-2968-3
[10] SMITH D N, DEVEY M E.Occurrence and inheritance of microsatellites in Pinus radiata[J]. Genome, 1994, 37(6): 977-983. DOI: 10.1139/g94-138
[11] PIPERIDIS G, PIPERIDIS N, D’HONT A. Molecular cytogenetic investigation of chromosome composition and transmission in sugarcane[J]. Molecular Genetics and Genomics, 2010, 284(1): 65-73. DOI: 10.1007/s00438-010-0546-3
[12] 雷刚, 陈学军, 周坤华, 等. 60份辣椒骨干亲本的SSR遗传多样性分析及指纹图谱构建[J]. 植物遗传资源学报, 2024, 25(08): 1321-1335.
LEI G, CHEN X J, ZHOU K H, et al.Genetic diversity and fingerprint analysis of 60 pepper
13 founder parents based on SSR markers[J]. Journal of Plant Genetic Resources, 2024, 25(8): 1321-1335. DOI: 10.13430/j.cnki.jpgr.20230830002.
[13] 李丹丹, 翁启杰, 甘四明, 等. 基于EST-SSR标记鉴定猴耳环自由授粉的全同胞子代[J]. 南京林业大学学报(自然科学版), 2022, 46(4): 95-101.
LI D D, WENG Q J, GAN S M, et al.Identification of full-sib seedlings from an open-pollinated family of Archidendron clypearia based on EST-SSR markers[J]. Journal of Nanjing Forestry University (Natural Science Edition), 2022, 46(4): 95-101. DOI:10.12302 /j.issn.1000-2006.202107029
[14] LI J, Zeng Y, Shen D, Xia G, et al.Development of SSR markers in hickory (Carya cathayensis Sarg.) and their transferability to other species of Carya[J]. Current Genomics. 2014 ,15(5): 357-79. DOI: 10.2174/138920291505141106103734
[15] 齐建勋, 王克建, 吴春林, 等. 核桃EST-SSR标记的开发[J]. 农业生物技术学报, 2009, 17(5): 872-876.
QI J S, WANG K J, WU C L, et al.Development of EST-SSR markers in Juglans regia[J]. Journal of Agricultural Biotechnology, 2009, 17(5): 872-876.
[16] 戴晓港,房芝馨,胡雪纯,等.薄壳山核桃全基因组SSR分析及多态性引物筛选[J].分子植物育种,2021,19(24):8199-8207.
DAI X G, FANG Z X, HU X C, et al.Whole genome SSR analysis and polymorphic primer screening in Carya illinoinensis[J]. Molecular Plant Breeding, 2019, 19(24): 8199-8207. DOI: 10.13271/j.mpb.019.008199.
[17] MASOUDZADEH N, TEIMOURIAN S.Comparison of quantitative fluorescent polymerase chain reaction and karyotype analysis for prenatal screening of chromosomal aneuploidies in 270 amniotic fluid samples[J]. Journal of Perinatal Medicine, 2019, 47(6): 631-636. DOI: 10.1515/jpm-2019-0069
[18] PEAKALL R, SMOUSE P E, GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research--an update[J]. Bioinformatics, 2012, 28(19): 2537-2539. DOI: 10.1093/bioinformatics/bts460
[19] MARSHALL T C, SLATE L J, KRUUK L E B, et al. Statistical confidence for likelihood-based paternity inference in natural populations[J]. Molecular Ecology, 1998,7(5): 639-655. DOI: 10.1046/j.
1365-294x.1998.00374.x
[20] JONES A G, SMALL C M, PACZOLT K A, et al.A practical guide to methods of parentage analysis[J]. Molecular Ecology Resources, 2010, 10(1): 6-30. DOI: 10.1111/j.1755-0998.2009.02778.x
[21] 杨晓伟, 朱宁华, 韩志强, 等. 铁心杉半同胞子代优株父本鉴定[J]. 中南林业科技大学学报, 2021, 41(12): 26-35.
YANG X W, ZHU N H, HANG Z Q, et al.Identification of paternal parents of half-sib progeny superior plants of the black-heart wood Chinese fir[J]. Journal of Central South University of Forestry and Technology, 2021, 41(12): 26-35. DOI: 10.14067/j.cnki.1673-923x.2021.12.004
[22] BOTSTEIN D, WHITE RL, SKOLNICK M, et al.Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J]. American Journal of Huma Genetics, 1980, 32(3): 314.
[23] 王斯琪, 唐诗哲, 孔德仓, 等. 利用SSR标记进行枣树子代苗父本鉴定[J]. 园艺学报, 2012, 39(11): 2133-2141.
WANG S Q, TANG S Z, KONG D C, et al.Application of SSR markers for the identification of paternal parent for the seedlings of Chinese jujube[J]. Chinese Journal of Horticulture, 2012, 39(11): 2133-2141. DOI: 10.16420/j.issn.0513-353x.2012.11.009
[24] 郑崇文, 王婉尧, 白斌, 等. 利用微卫星标记鉴定核桃杂交子代的亲本[J]. 四川林业科技, 2024, 45(2): 103-108.
ZHENG C W, WANG W Y, BAI B, et al.Parentage assignment of walnut hybrid offspring by microsatellite markers[J]. Sichuan Forestry Science and Technology,2024,45(2): 103-108. DOI: 10.12172/202209210002
[25] 周文才, 侯静, 郭炜, 等. 基于SSR标记的美洲黑杨杂交子代的鉴定[J]. 南京林业大学学报(自然科学版), 2015, 39(3): 45-49.
ZHOU W C, HOU J, GUO W, et al.Identification of the true hybrids for Populus deltoides by using SSR markers[J]. Journal of Nanjing Forestry University (Natural Science Edition), 2015, 39(3): 45-49. DOI: 10.3969/j.issn.1000-2006.2015.03.009.
[26] SHAW D V, BROWN A H D. Optimum number of marker loci for estimating outcrossing in plant populations[J]. Theoretical And Applied Genetics, 1982, 61(4): 321-325.DOI: 10.1007/BF00272848.
[27] 何田华, 葛颂 . 植物种群交配系统、亲本分析以及基因流动研究[J]. 植物生态学报 ,2001, 25(2): 144-154.
HE T H, GE S.Mating system, paternity analysis and gene flow in plant populations[J]. Acta Phytoecologica Sinica, 2001, 25(2): 144-154.
[28] MASHALL T C, SLATE J, KRUUK L E, et al.Statistical confidence for likelihood -based paternity inference in natural populations[J]. Molecular Ecology, 1998, 7(5): 639. DOI:10.1046/j.1365-294x.1998.00374.x
[29] 杨军, 孔祥瑞, 郑国华, 等. 茶树品种‘金牡丹’自然杂交后代遗传鉴定[J]. 热带亚热带植物学报, 2023,31(1): 69-80.
YANG J, KONG X R, ZHENG G H, et al.Genetic Identification of the natural hybrid progenies of‘Jinmudan’ tea varieties[J]. Journal of Tropical and Subtropical Botany, 2023, 31(1): 69-80. DOI: 10.11926/jtsb.4557
[30] 王楠楠, 李金花, 王长海, 等. 基于SSR标记的父本分析研究油橄榄品种间的亲和性[J]. 林业科学研究, 2017, 30(4): 640-647.
WANG N N, LI J H, WANG C H, et al.Paternity analysis using microsatellite markers to identify the pollen donors of olive open-pollinated offsprings[J]. Research in Forestry Sciences, 2017, 30(4): 640-647. DOI: 10.13275/j.cnki.lykxyj.2017.04.015
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