【Objective】This research aims to evaluate the fruit characteristics of 46 blueberry cultivars for selection and breeding purposes. 【Method】The fruit appearance, inner quality and sensory properties of 46 blueberry cultivars were investigated, then classified and evaluated by correlation, principal component and cluster analyses. 【Result】The northern highbush blueberry exhibited the largest fruit size, highest and acidity levels, and achieved the highest sensory score. The fruit of southern highbush blueberry was the smallest, with the lowest hardness and sensory score, the highest sweetness and total polyphenol content. The fruit of rabbit eye blueberry was larger than southern highbush blueberry, with the highest pericarp ratio and total anthocyanin content. Among appearance indices, the chromaticity a^* of fruit with epicuticular powder removed exhibited the highest coefficient of variation (CV) at 2 926.91%, followed closely by the original fruit chromaticity a^* at 370.96%. The CV of solid acid ratio (42.77%) was the highest among quality indices, followed by total anthocyanin content (39.78%), and the CV of sensory score was the lowest (<10%). Correlation analysis revealed that larger fruit size was associated with lower pericarp percentage and total polyphenol content. The acidity had the greatest effect on the solid acid ratio of blueberry fruits. Sensory evaluation indices, including appearance and comprehensive scores, showed significant positive correlations with fruit weight, transverse diameter, and longitudinal diameter, indicating that larger fruit size is a preferred characteristic in sensory evaluation. Cluster analysis showed that the 46 blueberry cultivars were divided into three types, among which the first type had the largest fruit with high hardness and acidity, the second type had the second-largest fruit with the highest sweetness, and the third type had the smallest fruit with high pericarp ratio, belonging to the processing type with high total anthocyanin and polyphenol content. Principal component analysis reduced the 11 appearance and quality indices to four principal components, explaining 75.217% of the total variance. Sensory evaluation revealed that, in addition to differences in size, sweet-sour balance and nutrient content, the 46 blueberry cultivars also varied in wax thickness, texture and fruity aroma, and seven large-fruit cultivars of ‘Primadonna’, ‘Millennia’, ‘Jewel’, ‘Chandler’, ‘Brigitta’, ‘Aurora’ and ‘Prolific’, 3 high sweetness cultivars of ‘Anna’ ‘Sweetheart’ and ‘Brightwell’, and four excellent taste cultivars of ‘Sunshine’, ‘Magnolia’, ‘Brigitta’ and ‘Beckyblue’ were selected. Comprehensive evaluation showed that sensory assessment results were largely consistent with principal component and cluster analyses. 【Conclusion】The 10 cultivars with large fruit size, high sweetness, crisp texture and rich aroma were ‘Prolific’, ‘Chandler’, ‘Sweetheart’, ‘Brigitta’, ‘Millennia’, ‘Beckyblue’, ‘Primadonna’, ‘Brigitta’, ‘Magnolia’ and ‘Pink Lemonade’, which were recommended for fresh consumption, and the 5 cultivars with high total anthocyanins and polyphenols content and high pericarp percent were ‘Gardenblue’, ‘Zhongzhi 4’, ‘Zhaixuan 4’, ‘Baldwin’ and ‘Centurion’, which can be used for processing blueberry products. These cultivars can also serve as parental materials for corresponding breeding objectives.