The effects of boron deficiency on physiology and boron transport-related genes expression of Camellia oleifera

ZHANG Mengxue, LIU Junying, FAN Jie, DING Wenna, LUO Jie, MEI Li

Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2026, Vol. 50 ›› Issue (2) : 119-128.

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南京林业大学学报（自然科学版）
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Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2026, Vol. 50 ›› Issue (2) : 119-128. DOI: 10.12302/j.issn.1000-2006.202409045

The effects of boron deficiency on physiology and boron transport-related genes expression of Camellia oleifera

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Abstract

【Objective】This study aims to screen boron (B) deficiency tolerant Camellia oleifera cultivars, analyze the physiology of B deficiency tolerance and the molecular mechanism of B transport in C. oleifera.【Method】The physiological responses of seven C. oleifera cultivars seedlings under B deficiency stress were investigated. The affiliation function value method was used to evaluate the resistance of different cultivars to B deficiency. And the tolerance differences between B-efficient and B-inefficient C. oleifera cultivars from the perspective of four B transporter gene expression were analyzed.【Result】(1) Under the B deficiency stress environment, the symptoms of seven cultivars were yellowing and curling of leaves, browning and shedding of roots, and stunting of growth. (2) B deficiency stress reduced the net photosynthetic rate and other photosynthetic parameters, and the B content in some C. oleifera cultivars decreased significantly. The B deficiency reduced the content and activity of osmotic regulators such as soluble sugars, hydrogen peroxide and superoxide anions in leaves and roots, as well as superoxide dismutase. (3) The results of membership function analysis showed that ‘Changlin 40’ and ‘Changlin 4’ were more tolerant to B deficiency, while ‘Huajin’ was the least tolerant to B deficiency. (4) The expressions of four B transporter genes (CoBOR1, CoBOR1-like, CoBOR2, andCoNIP5;1) in ‘Changlin 4’ and ‘Huajin’ were significantly up-regulated, and the expression levels of the four genes in the roots and leaves of the B-deficiency-tolerant cultivar ‘Changlin 4’ were significantly higher than those in the B-deficiency-tolerant cultivar ‘Huajin’.【Conclusion】The tolerance of different C. oleifera cultivars to B deficiency stress varies greatly. The B-efficient cultivars could enhance their ability of boron deficiency tolerance by up-regulating the expression of B transport genes, improving photosynthetic efficiency, antioxidant capacity and osmotic regulation capacity.

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Camellia oleifera / boron deficiency stress / photosynthetic efficiency / antioxidant enzymes / boron transporter genes

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ZHANG Mengxue , LIU Junying , FAN Jie , et al . The effects of boron deficiency on physiology and boron transport-related genes expression of Camellia oleifera[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2026, 50(2): 119-128 https://doi.org/10.12302/j.issn.1000-2006.202409045

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