【Objective】This study aims to develop a complete somatic embryogenesis system for Tilia cordata by systematically optimizing the culture medium composition to identify optimal conditions for somatic embryo induction and subsequent plant regeneration.【Method】Immature zygotic embryos of T. cordata were used as explants. The effects of different concentrations and combinations of plant growth regulators 2,4-dichlorophenoxyacetic acid(2,4-D and 6-benzylaminopurine(6-BA) on callus induction, proliferation, and somatic embryo induction efficiency were systematically investigated. Additionally, the impacts of different basal media, indole-3-butyric acid(IBA) concentrations, and activated charcoal supplementation on somatic embryo germination and plant regeneration were evaluated.【Result】Optimal callus induction was achieved at a concentration of 3.0 mg/L 2,4-D combined with 0.2 mg/L 6-BA, yielding an induction efficiency of 64.25%. Subsequent proliferation of embryogenic callus demonstrated maximal growth potential under identical hormonal conditions (3.0 mg/L 2,4-D+0.2 mg/L 6-BA), as evidenced by an exceptional proliferation coefficient of 1 063.72%. For somatic embryo induction, the same hormonal formulation (3.0 mg/L 2,4-D+0.2 mg/L 6-BA) proved most effective, generating somatic embryos at a frequency of 46.41%. Complete plant regeneration was successfully accomplished through transfering of developed somatic embryos to MS basal medium supplemented with 0.5 mg/L IBA in the absence of activated charcoal, with all regenerated plants exhibiting normal morphological characteristics.【Conclusion】In this study, a complete somatic embryogenesis system of T. cordata was initially established, providing a basis for subsequent establishment of an efficient propagation and genetic transformation system for Tilia.