【Objective】Functional microorganisms play crucial roles in facilitating soil restoration and ecosystem reconstruction. This study aims to establish a method for labeling the highly efficient silicate-dissolving bacterial strain Pseudomonas protegens NLX-4 with an antibiotic resistance plasmid by introducing exogenous plasmids to evaluate the reproductive capacity, survival ability, and other characteristics of different strains in natural environments. The research further investigates the effects of genetic modification on strain performance, providing a foundation for the broader application of functional strains in geotechnical improvement.【Method】The silicate-dissolving bacterial strain P. protegens NLX-4 was used as the experimental strain.A plasmid was introduced via electroporation to generate a novel mutant strain with ampicillin resistance.The ampicillin resistance was tested using the dilution coating method at three gradients(10⁴,10⁵,10⁶).Growth capabilities were compared between the mutant and original strains through plate growth assays and growth curve measurements (OD₆₀₀ values recorded at 2-hour intervals).Soil mechanical particle size changes in plant rhizospheres were analyzed via pot experiments,with dissolution capacity differences evaluated using enrichment rates and average mass diameter indices.【Result】Compared to the original strain P. protegens NLX-4,the mutant strain P. protegens NLX-41(OD₆₀₀=1) successfully acquire the ampicillin resistance gene and form colonies on ampicillin resistance plates at all concentration gradients.In terms of growth rate,both the mutant strain NLX-41 and the original strain reach the peak bacterial concentration at 36 h,with the absorbance stabilizing at approximately 2.8.In terms of dissolution ability,the contents of silt,very fine sand,and fine sand in the NLX-41 group and NLX-4 group show significant differences compared with the control group(P<0.01).The average weight diameter of the mutant strain is 96.46% of that of the original strain.Under the treatment of the original strain and the mutant strain,the enrichment rates of medium sand decrease by 19% and 24%,respectively,while the enrichment rates of fine sand increase by 20% and 25% respectively.【Conclusion】A mutant strain,NLX-41, is obtained via electroporation,retaining high-efficiency growth and dissolution capabilities while exhibiting ampicillin resistance.This method enhances the screening efficiency of the strain,facilitating rapid identification of functional microorganisms.Additionally,the established genetic transformation protocol enables future insertion of target genes under diverse scenarios,expanding its application potential.